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. 1988 Jul;32(7):1103–1105. doi: 10.1128/aac.32.7.1103

Rapid determination of amphotericin B levels in serum by high-performance liquid chromatography without interference by bilirubin.

H Hosotsubo 1, J Takezawa 1, N Taenaka 1, K Hosotsubo 1, I Yoshiya 1
PMCID: PMC172355  PMID: 3190197

Abstract

A high-performance liquid chromatographic method for the determination of amphotericin B concentrations in human serum without bilirubin interference was developed and compared with a microbiological assay. The high-performance liquid chromatographic assay utilized a reversed-phase trimethyl silica column, simple sample preparation, and visible detection. Reproducibility studies yielded coefficient-of-variation ranges from 1.02 to 2.11% for within-day precision and 2.88 to 4.32% for between-day precision. The correlation coefficient with the microbiological assay was 0.984 for amphotericin B.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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