Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1988 Aug;32(8):1131–1136. doi: 10.1128/aac.32.8.1131

MIC as a quantitative measurement of the susceptibility of Mycobacterium avium strains to seven antituberculosis drugs.

L Heifets 1
PMCID: PMC172364  PMID: 2461162

Abstract

MICs of isoniazid, rifampin, ethionamide, streptomycin, amikacin, kanamycin, and capreomycin were determined for Mycobacterium avium complex strains by two methods: broth dilution in 7H12 medium radiometrically and agar dilution on 7H10 agar plates. The broth-determined MICs of all drugs with the exception of isoniazid were two to eight times lower than the agar-determined MICs for most of the tested M. avium strains, which is probably due to the higher absorption and degradation of the drugs in solid media. The MICs, especially those determined in broth, are suggested as quantitative measurements of the degree of susceptibility of M. avium complex strains. For a certain percentage of the M. avium strains the broth-determined MICs were within the limits of MICs found for wild susceptible Mycobacterium tuberculosis strains. These M. avium strains were classified as presumably susceptible. In contrast to M. tuberculosis, the MICs for M. avium strains had a wide range. When the MICs for M. avium strains were only one dilution higher than those for M. tuberculosis, they were tentatively classified as moderately susceptible. The designation moderately resistant or resistant, respectively, is suggested for those M. avium strains for which the MICs were at or above the concentrations achievable in blood. The quantitation of the degree of susceptibility by the MICs and the tentative interpretation of the MICs are suggested for future use in clinical trials as a means of evaluating the patients' responses to chemotherapy compared with the degree of susceptibility of the initial strain isolated before treatment.

Full text

PDF
1131

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beggs W. H., Andrews F. A. Role of ionic strength in salt antagonism of aminoglycoside action on Escherichia coli and Pseudomonas aeruginosa. J Infect Dis. 1976 Nov;134(5):500–504. doi: 10.1093/infdis/134.5.500. [DOI] [PubMed] [Google Scholar]
  2. CANETTI G., FROMAN S., GROSSET J., HAUDUROY P., LANGEROVA M., MAHLER H. T., MEISSNER G., MITCHISON D. A., SULA L. MYCOBACTERIA: LABORATORY METHODS FOR TESTING DRUG SENSITIVITY AND RESISTANCE. Bull World Health Organ. 1963;29:565–578. [PMC free article] [PubMed] [Google Scholar]
  3. Canetti G., Fox W., Khomenko A., Mahler H. T., Menon N. K., Mitchison D. A., Rist N., Smelev N. A. Advances in techniques of testing mycobacterial drug sensitivity, and the use of sensitivity tests in tuberculosis control programmes. Bull World Health Organ. 1969;41(1):21–43. [PMC free article] [PubMed] [Google Scholar]
  4. Cynamon M. H. Comparative in vitro activities of MDL 473, rifampin, and ansamycin against Mycobacterium intracellulare. Antimicrob Agents Chemother. 1985 Sep;28(3):440–441. doi: 10.1128/aac.28.3.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davis C. E., Jr, Carpenter J. L., Trevino S., Koch J., Ognibene A. J. In vitro susceptibility of Mycobacterium avium complex to antibacterial agents. Diagn Microbiol Infect Dis. 1987 Nov;8(3):149–155. doi: 10.1016/0732-8893(87)90165-9. [DOI] [PubMed] [Google Scholar]
  6. Donovick R., Bayan A. P., Canales P., Pansy F. The Influence of Certain Substances on the Activity of Streptomycin: III. Differential Effects of Various Electrolytes on the Action of Streptomycin. J Bacteriol. 1948 Jul;56(1):125–137. [PMC free article] [PubMed] [Google Scholar]
  7. Heifets L. B., Iseman M. D., Lindholm-Levy P. J. Determination of MICs of conventional and experimental drugs in liquid medium by the radiometric method against Mycobacterium avium complex. Drugs Exp Clin Res. 1987;13(9):529–538. [PubMed] [Google Scholar]
  8. Heifets L. B., Iseman M. D., Lindholm-Levy P. J. Ethambutol MICs and MBCs for Mycobacterium avium complex and Mycobacterium tuberculosis. Antimicrob Agents Chemother. 1986 Dec;30(6):927–932. doi: 10.1128/aac.30.6.927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Heifets L. B., Iseman M. D., Lindholm-Levy P. J., Kanes W. Determination of ansamycin MICs for Mycobacterium avium complex in liquid medium by radiometric and conventional methods. Antimicrob Agents Chemother. 1985 Oct;28(4):570–575. doi: 10.1128/aac.28.4.570. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heifets L. B., Lindholm-Levy P. J. Bacteriostatic and bactericidal activity of ciprofloxacin and ofloxacin against Mycobacterium tuberculosis and Mycobacterium avium complex. Tubercle. 1987 Dec;68(4):267–276. doi: 10.1016/0041-3879(87)90067-5. [DOI] [PubMed] [Google Scholar]
  11. Heifets L. B., Lindholm-Levy P. J., Iseman M. D. Rifabutine: minimal inhibitory and bactericidal concentrations for Mycobacterium tuberculosis. Am Rev Respir Dis. 1988 Mar;137(3):719–721. doi: 10.1164/ajrccm/137.3.719. [DOI] [PubMed] [Google Scholar]
  12. Holdiness M. R. Clinical pharmacokinetics of the antituberculosis drugs. Clin Pharmacokinet. 1984 Nov-Dec;9(6):511–544. doi: 10.2165/00003088-198409060-00003. [DOI] [PubMed] [Google Scholar]
  13. Inderlied C. B., Young L. S., Yamada J. K. Determination of in vitro susceptibility of Mycobacterium avium complex isolates to antimycobacterial agents by various methods. Antimicrob Agents Chemother. 1987 Nov;31(11):1697–1702. doi: 10.1128/aac.31.11.1697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lee C. N., Heifets L. B. Determination of minimal inhibitory concentrations of antituberculosis drugs by radiometric and conventional methods. Am Rev Respir Dis. 1987 Aug;136(2):349–352. doi: 10.1164/ajrccm/136.2.349. [DOI] [PubMed] [Google Scholar]
  15. Snider D. E., Jr, Good R. C., Kilburn J. O., Laskowski L. F., Jr, Lusk R. H., Marr J. J., Reggiardo Z., Middlebrook G. Rapid drug-susceptibility testing of Mycobacterium tuberculosis. Am Rev Respir Dis. 1981 Apr;123(4 Pt 1):402–406. doi: 10.1164/arrd.1981.123.4.402. [DOI] [PubMed] [Google Scholar]
  16. Tarrand J. J., Gröschel D. H. Evaluation of the BACTEC radiometric method for detection of 1% resistant populations of Mycobacterium tuberculosis. J Clin Microbiol. 1985 Jun;21(6):941–946. doi: 10.1128/jcm.21.6.941-946.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tsukamura M. Evidence that antituberculosis drugs are really effective in the treatment of pulmonary infection caused by Mycobacterium avium complex. Am Rev Respir Dis. 1988 Jan;137(1):144–148. doi: 10.1164/ajrccm/137.1.144. [DOI] [PubMed] [Google Scholar]
  18. WEINBERG E. D. The mutual effects of antimicrobial compounds and metallic cations. Bacteriol Rev. 1957 Mar;21(1):46–68. doi: 10.1128/br.21.1.46-68.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wolinsky E. Nontuberculous mycobacteria and associated diseases. Am Rev Respir Dis. 1979 Jan;119(1):107–159. doi: 10.1164/arrd.1979.119.1.107. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES