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. 1988 Aug;32(8):1164–1169. doi: 10.1128/aac.32.8.1164

Tn4201, a beta-lactamase transposon in Staphylococcus aureus.

D A Weber 1, R V Goering 1
PMCID: PMC172370  PMID: 2847645

Abstract

We report the preliminary characterization of a 6.7-kilobase transposon, Tn4201, encoding resistance to penicillin associated with the conjugative plasmid pCRG1600 in Staphylococcus aureus. Tn4201 is capable of inverting in orientation (frequently observed in derivatives of pCRG1600) and is expressed equally well in either orientation. The element is also capable of movement to alternative plasmid and chromosomal sites. Tn4201 exhibits rec-independent transposition in an apparent site-specific manner and may play an important role in the resistance of staphylococci to beta-lactam compounds.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asch D. K., Goering R. V., Ruff E. A. Isolation and preliminary characterization of a plasmid mutant derepressed for conjugal transfer in Staphylococcus aureus. Plasmid. 1984 Nov;12(3):197–202. doi: 10.1016/0147-619x(84)90044-1. [DOI] [PubMed] [Google Scholar]
  2. Brown D. T., Brown N. C., Burlingham B. T. Morphology and physical properties of Staphylococcus bacteriophage P11-M15. J Virol. 1972 Apr;9(4):664–671. doi: 10.1128/jvi.9.4.664-671.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chan P. T. Nucleotide sequence of the Staphylococcus aureus PC1 beta-lactamase gene. Nucleic Acids Res. 1986 Jul 25;14(14):5940–5940. doi: 10.1093/nar/14.14.5940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goering R. V. Mutants of Staphylococcus aureus deficient in recombinational repair. Improved isolation by selecting for mutants exhibiting concurrent sensitivity to ultraviolet radiation and N-methyl-N'-nitro-N-nitrosoguanidine. Mutat Res. 1979 May;60(3):279–289. doi: 10.1016/0027-5107(79)90018-6. [DOI] [PubMed] [Google Scholar]
  5. Goering R. V., Ruff E. A. Comparative analysis of conjugative plasmids mediating gentamicin resistance in Staphylococcus aureus. Antimicrob Agents Chemother. 1983 Sep;24(3):450–452. doi: 10.1128/aac.24.3.450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Holmes D. S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. doi: 10.1016/0003-2697(81)90473-5. [DOI] [PubMed] [Google Scholar]
  7. KLOSS W. E., PATTEE P. A. TRANSDUCTION ANALYSIS OF THE HISTIDINE REGION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1965 May;39:195–207. doi: 10.1099/00221287-39-2-195. [DOI] [PubMed] [Google Scholar]
  8. Kleckner N. Transposable elements in prokaryotes. Annu Rev Genet. 1981;15:341–404. doi: 10.1146/annurev.ge.15.120181.002013. [DOI] [PubMed] [Google Scholar]
  9. Leary J. J., Brigati D. J., Ward D. C. Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. Proc Natl Acad Sci U S A. 1983 Jul;80(13):4045–4049. doi: 10.1073/pnas.80.13.4045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lyon B. R., May J. W., Skurray R. A. Tn4001: a gentamicin and kanamycin resistance transposon in Staphylococcus aureus. Mol Gen Genet. 1984;193(3):554–556. doi: 10.1007/BF00382099. [DOI] [PubMed] [Google Scholar]
  11. Lyon B. R., Skurray R. Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev. 1987 Mar;51(1):88–134. doi: 10.1128/mr.51.1.88-134.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Murphy E., Novick R. P. Physical mapping of Staphylococcus aureus penicillinase plasmid pI524: characterization of an invertible region. Mol Gen Genet. 1979 Aug;175(1):19–30. doi: 10.1007/BF00267851. [DOI] [PubMed] [Google Scholar]
  14. Novick R. P., Edelman I., Schwesinger M. D., Gruss A. D., Swanson E. C., Pattee P. A. Genetic translocation in Staphylococcus aureus. Proc Natl Acad Sci U S A. 1979 Jan;76(1):400–404. doi: 10.1073/pnas.76.1.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. PATTEE P. A., BALDWIN J. N. Transduction of resistance to chlortetracycline and novobiocin in Staphylococcus aureus. J Bacteriol. 1961 Dec;82:875–881. doi: 10.1128/jb.82.6.875-881.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pattee P. A., Neveln D. S. Transformation analysis of three linkage groups in Staphylococcus aureus. J Bacteriol. 1975 Oct;124(1):201–211. doi: 10.1128/jb.124.1.201-211.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Phillips S., Novick R. P. Tn554--a site-specific repressor-controlled transposon in Staphylococcus aureus. Nature. 1979 Mar 29;278(5703):476–478. doi: 10.1038/278476a0. [DOI] [PubMed] [Google Scholar]
  18. Wang P. Z., Novick R. P. Nucleotide sequence and expression of the beta-lactamase gene from Staphylococcus aureus plasmid pI258 in Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. J Bacteriol. 1987 Apr;169(4):1763–1766. doi: 10.1128/jb.169.4.1763-1766.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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