Abstract
The effectiveness of nanoparticle-bound ampicillin was tested in the treatment of experimental Listeria monocytogenes infection in congenitally athymic nude mice. Nanoparticles of polyisohexylcyanoacrylate (PIHCA) 187 +/- 13 nm in diameter were bound to ampicillin at an ampicillin/PIHCA ratio of 0.2:1. The proportion of ampicillin bound was 90% +/- 3%. After adsorption onto nanoparticles, the therapeutic activity of ampicillin increased dramatically over that in the free state. Thus, 2.4 mg of nanoparticle-bound ampicillin (three doses of 0.8 mg each) had a greater therapeutic effect than 48 mg of free ampicillin (three doses of 16 mg each). These results might provide an incentive for further development of intracellular targeting of antibiotics on biodegradable polymeric carriers.
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Selected References
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- Bakker-Woudenberg I. A., Lokerse A. F., Roerdink F. H., Regts D., Michel M. F. Free versus liposome-entrapped ampicillin in treatment of infection due to Listeria monocytogenes in normal and athymic (nude) mice. J Infect Dis. 1985 May;151(5):917–924. doi: 10.1093/infdis/151.5.917. [DOI] [PubMed] [Google Scholar]
- Bakker-Woudenberg I. A., Lokerse A. F., Vink-van den Berg J. C., Roerdink F. H., Michel M. F. Effect of liposome-entrapped ampicillin on survival of Listeria monocytogenes in murine peritoneal macrophages. Antimicrob Agents Chemother. 1986 Aug;30(2):295–300. doi: 10.1128/aac.30.2.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Couvreur P., Kante B., Roland M., Speiser P. Adsorption of antineoplastic drugs to polyalkylcyanoacrylate nanoparticles and their release in calf serum. J Pharm Sci. 1979 Dec;68(12):1521–1524. doi: 10.1002/jps.2600681215. [DOI] [PubMed] [Google Scholar]
- Gipps E. M., Arshady R., Kreuter J., Groscurth P., Speiser P. P. Distribution of polyhexyl cyanoacrylate nanoparticles in nude mice bearing human osteosarcoma. J Pharm Sci. 1986 Mar;75(3):256–258. doi: 10.1002/jps.2600750310. [DOI] [PubMed] [Google Scholar]
- Horwitz M. A. Phagocytosis of microorganisms. Rev Infect Dis. 1982 Jan-Feb;4(1):104–123. doi: 10.1093/clinids/4.1.104. [DOI] [PubMed] [Google Scholar]
- Kante B., Couvreur P., Dubois-Krack G., De Meester C., Guiot P., Roland M., Mercier M., Speiser P. Toxicity of polyalkylcyanoacrylate nanoparticles I: Free nanoparticles. J Pharm Sci. 1982 Jul;71(7):786–790. doi: 10.1002/jps.2600710716. [DOI] [PubMed] [Google Scholar]
- Kimelberg H. K., Mayhew E., Papahadjopoulos D. Distribution of liposome-entrapped cations in tumor-bearing mice. Life Sci. 1975 Sep 1;17(5):715–723. doi: 10.1016/0024-3205(75)90526-3. [DOI] [PubMed] [Google Scholar]
- Lenaerts V., Nagelkerke J. F., Van Berkel T. J., Couvreur P., Grislain L., Roland M., Speiser P. In vivo uptake of polyisobutyl cyanoacrylate nanoparticles by rat liver Kupffer, endothelial, and parenchymal cells. J Pharm Sci. 1984 Jul;73(7):980–982. doi: 10.1002/jps.2600730730. [DOI] [PubMed] [Google Scholar]
- Nieman R. E., Lorber B. Listeriosis in adults: a changing pattern. Report of eight cases and review of the literature, 1968-1978. Rev Infect Dis. 1980 Mar-Apr;2(2):207–227. doi: 10.1093/clinids/2.2.207. [DOI] [PubMed] [Google Scholar]
- Stevenson M., Baillie A. J., Richards R. M. Enhanced activity of streptomycin and chloramphenicol against intracellular Escherichia coli in the J774 macrophage cell line mediated by liposome delivery. Antimicrob Agents Chemother. 1983 Nov;24(5):742–749. doi: 10.1128/aac.24.5.742. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vladimirsky M. A., Ladigina G. A. Antibacterial activity of liposome-entrapped streptomycin in mice infected with mycobacterium tuberculosis. Biomed Pharmacother. 1982;36(8-9):375–377. [PubMed] [Google Scholar]