Skip to main content
NCBI home page
The British Journal of Ophthalmology logoLink to The British Journal of Ophthalmology
. 2001 Dec;85(12):1426–1428. doi: 10.1136/bjo.85.12.1426

Plasma malondialdehyde and nitric oxide levels in age related macular degeneration

Y Totan 1, O Cekic 1, M Borazan 1, E Uz 1, S Sogut 1, O Akyol 1
PMCID: PMC1723807  PMID: 11734513

Abstract

AIMS—To evaluate alteration of plasma malondialdehyde (MDA) and nitric oxide (NO) levels in patients with exudative age related macular degeneration (ARMD).
METHODS—Plasma nitrite plus nitrate concentrations as an index of plasma NO levels and plasma MDA level as a marker of lipid peroxidation were measured in patients with exudative ARMD and age and sex matched healthy subjects.
RESULTS—Significantly higher MDA and lower NO levels were detected in plasma of patients with ARMD compared with their controls (p=0.01, p=0.001, respectively).
CONCLUSION—The results may support involvement of oxidative damage and vascular theory in the pathogenesis of ARMD as part of the ageing process.



Full Text

The Full Text of this article is available as a PDF (110.3 KB).

Figure 1  .

Figure 1  

Open in a new tab

Plot of plasma malondialdehyde (MDA) levels in patients with age related macular degeneration (ARMD) and control subjects.

Figure 2  .

Figure 2  

Open in a new tab

Plot of plasma nitrite plus nitrate levels in patients with age related macular degeneration (ARMD) and control subjects.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames B. N., Shigenaga M. K., Hagen T. M. Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):7915–7922. doi: 10.1073/pnas.90.17.7915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown K. M., Morrice P. C., Duthie G. G. Vitamin E supplementation suppresses indexes of lipid peroxidation and platelet counts in blood of smokers and nonsmokers but plasma lipoprotein concentrations remain unchanged. Am J Clin Nutr. 1994 Sep;60(3):383–387. doi: 10.1093/ajcn/60.3.383. [DOI] [PubMed] [Google Scholar]
  3. Cai J., Nelson K. C., Wu M., Sternberg P., Jr, Jones D. P. Oxidative damage and protection of the RPE. Prog Retin Eye Res. 2000 Mar;19(2):205–221. doi: 10.1016/s1350-9462(99)00009-9. [DOI] [PubMed] [Google Scholar]
  4. Ciulla T. A., Harris A., Chung H. S., Danis R. P., Kagemann L., McNulty L., Pratt L. M., Martin B. J. Color Doppler imaging discloses reduced ocular blood flow velocities in nonexudative age-related macular degeneration. Am J Ophthalmol. 1999 Jul;128(1):75–80. doi: 10.1016/s0002-9394(99)00061-6. [DOI] [PubMed] [Google Scholar]
  5. Cohen S. M., Olin K. L., Feuer W. J., Hjelmeland L., Keen C. L., Morse L. S. Low glutathione reductase and peroxidase activity in age-related macular degeneration. Br J Ophthalmol. 1994 Oct;78(10):791–794. doi: 10.1136/bjo.78.10.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cortas N. K., Wakid N. W. Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. Clin Chem. 1990 Aug;36(8 Pt 1):1440–1443. [PubMed] [Google Scholar]
  7. Coudray C., Roussel A. M., Arnaud J., Favier A. Selenium and antioxidant vitamin and lipidoperoxidation levels in preaging French population. EVA Study Group. Edude de vieillissement artériel. Biol Trace Elem Res. 1997 May;57(2):183–190. doi: 10.1007/BF02778201. [DOI] [PubMed] [Google Scholar]
  8. De La Paz M., Anderson R. E. Region and age-dependent variation in susceptibility of the human retina to lipid peroxidation. Invest Ophthalmol Vis Sci. 1992 Dec;33(13):3497–3499. [PubMed] [Google Scholar]
  9. Delcourt C., Cristol J. P., Léger C. L., Descomps B., Papoz L. Associations of antioxidant enzymes with cataract and age-related macular degeneration. The POLA Study. Pathologies Oculaires Liées à l'Age. Ophthalmology. 1999 Feb;106(2):215–222. doi: 10.1016/s0161-6420(99)90059-3. [DOI] [PubMed] [Google Scholar]
  10. Deng L. Y., Thibault G., Schiffrin E. L. Effect of hypertension induced by nitric oxide synthase inhibition on structure and function of resistance arteries in the rat. Clin Exp Hypertens. 1993 May;15(3):527–537. doi: 10.3109/10641969309041627. [DOI] [PubMed] [Google Scholar]
  11. Fliesler S. J., Anderson R. E. Chemistry and metabolism of lipids in the vertebrate retina. Prog Lipid Res. 1983;22(2):79–131. doi: 10.1016/0163-7827(83)90004-8. [DOI] [PubMed] [Google Scholar]
  12. Friedman E. A hemodynamic model of the pathogenesis of age-related macular degeneration. Am J Ophthalmol. 1997 Nov;124(5):677–682. doi: 10.1016/s0002-9394(14)70906-7. [DOI] [PubMed] [Google Scholar]
  13. Friedman E., Krupsky S., Lane A. M., Oak S. S., Friedman E. S., Egan K., Gragoudas E. S. Ocular blood flow velocity in age-related macular degeneration. Ophthalmology. 1995 Apr;102(4):640–646. doi: 10.1016/s0161-6420(95)30974-8. [DOI] [PubMed] [Google Scholar]
  14. Gallou G., Ruelland A., Legras B., Maugendre D., Allannic H., Cloarec L. Plasma malondialdehyde in type 1 and type 2 diabetic patients. Clin Chim Acta. 1993 Feb 28;214(2):227–234. doi: 10.1016/0009-8981(93)90114-j. [DOI] [PubMed] [Google Scholar]
  15. Goldstein I. M., Ostwald P., Roth S. Nitric oxide: a review of its role in retinal function and disease. Vision Res. 1996 Sep;36(18):2979–2994. doi: 10.1016/0042-6989(96)00017-x. [DOI] [PubMed] [Google Scholar]
  16. Harman D. Aging: phenomena and theories. Ann N Y Acad Sci. 1998 Nov 20;854:1–7. doi: 10.1111/j.1749-6632.1998.tb09886.x. [DOI] [PubMed] [Google Scholar]
  17. Harris A., Chung H. S., Ciulla T. A., Kagemann L. Progress in measurement of ocular blood flow and relevance to our understanding of glaucoma and age-related macular degeneration. Prog Retin Eye Res. 1999 Sep;18(5):669–687. doi: 10.1016/s1350-9462(98)00037-8. [DOI] [PubMed] [Google Scholar]
  18. Honing M. L., Morrison P. J., Banga J. D., Stroes E. S., Rabelink T. J. Nitric oxide availability in diabetes mellitus. Diabetes Metab Rev. 1998 Sep;14(3):241–249. doi: 10.1002/(sici)1099-0895(1998090)14:3<241::aid-dmr216>3.0.co;2-r. [DOI] [PubMed] [Google Scholar]
  19. Ito T., Nakano M., Yamamoto Y., Hiramitsu T., Mizuno Y. Hemoglobin-induced lipid peroxidation in the retina: a possible mechanism for macular degeneration. Arch Biochem Biophys. 1995 Feb 1;316(2):864–872. doi: 10.1006/abbi.1995.1116. [DOI] [PubMed] [Google Scholar]
  20. Koss M. C. Functional role of nitric oxide in regulation of ocular blood flow. Eur J Pharmacol. 1999 Jun 18;374(2):161–174. doi: 10.1016/s0014-2999(99)00242-3. [DOI] [PubMed] [Google Scholar]
  21. Liu S. X., Xuan B. O., Chen Z., Varma R. S., Chiou G. C. Nitric oxide donors: effects of S-nitrosoglutathione and 4-phenyl-3-furoxancarbonitrile on ocular blood flow and retinal function recovery. J Ocul Pharmacol Ther. 1997 Apr;13(2):105–114. doi: 10.1089/jop.1997.13.105. [DOI] [PubMed] [Google Scholar]
  22. Luksch A., Polak K., Beier C., Polska E., Wolzt M., Dorner G. T., Eichler H. G., Schmetterer L. Effects of systemic NO synthase inhibition on choroidal and optic nerve head blood flow in healthy subjects. Invest Ophthalmol Vis Sci. 2000 Sep;41(10):3080–3084. [PubMed] [Google Scholar]
  23. Nielsen F., Mikkelsen B. B., Nielsen J. B., Andersen H. R., Grandjean P. Plasma malondialdehyde as biomarker for oxidative stress: reference interval and effects of life-style factors. Clin Chem. 1997 Jul;43(7):1209–1214. [PubMed] [Google Scholar]
  24. Prashar S., Pandav S. S., Gupta A., Nath R. Antioxidant enzymes in RBCs as a biological index of age related macular degeneration. Acta Ophthalmol (Copenh) 1993 Apr;71(2):214–218. doi: 10.1111/j.1755-3768.1993.tb04993.x. [DOI] [PubMed] [Google Scholar]
  25. Schmetterer L., Krejcy K., Kastner J., Wolzt M., Gouya G., Findl O., Lexer F., Breiteneder H., Fercher A. F., Eichler H. G. The effect of systemic nitric oxide-synthase inhibition on ocular fundus pulsations in man. Exp Eye Res. 1997 Mar;64(3):305–312. doi: 10.1006/exer.1996.0213. [DOI] [PubMed] [Google Scholar]
  26. Stamler J. S., Loh E., Roddy M. A., Currie K. E., Creager M. A. Nitric oxide regulates basal systemic and pulmonary vascular resistance in healthy humans. Circulation. 1994 May;89(5):2035–2040. doi: 10.1161/01.cir.89.5.2035. [DOI] [PubMed] [Google Scholar]
  27. Violi F., Marino R., Milite M. T., Loffredo L. Nitric oxide and its role in lipid peroxidation. Diabetes Metab Res Rev. 1999 Jul-Aug;15(4):283–288. doi: 10.1002/(sici)1520-7560(199907/08)15:4<283::aid-dmrr42>3.0.co;2-u. [DOI] [PubMed] [Google Scholar]
  28. Wasowicz W., Nève J., Peretz A. Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clin Chem. 1993 Dec;39(12):2522–2526. [PubMed] [Google Scholar]
  29. Xuan B., Zhou Y. H., Varma R., Chiou G. C. Effects of some N-nitropyrazole derivatives on ocular blood flow and retinal function recovery after ischemic insult. J Ocul Pharmacol Ther. 1999 Apr;15(2):135–142. doi: 10.1089/jop.1999.15.135. [DOI] [PubMed] [Google Scholar]

Articles from The British Journal of Ophthalmology are provided here courtesy of BMJ Publishing Group

RESOURCES