Abstract
A highly cephem-resistant Escherichia coli strain, FP1546, isolated from the fecal flora of laboratory dogs previously administered beta-lactam antibiotics was found to produce a beta-lactamase, FEC-1, of 48-kilodalton size and pI 8.2. FEC-1 hydrolyzed cefuroxime, cefotaxime, cefmenoxime, and ceftriaxone, as well as the enzymatically less-stable antibiotics cephaloridine, cefotiam, and cefpiramide. Of the oxyimino-cephalosporins, ceftizoxime was fairly stable to FEC-1. FEC-1 differed notably from chromosomal E. coli cephalosporinase, especially in its broad-spectrum substrate profile and its high inhibition by clavulanic acid, sulbactam, and imipenem. A conjugation study revealed that FEC-1 was encoded by a 74-megadalton plasmid, pFCX1. This may be the first instance of a plasmid-mediated oxyimino-cephalosporinase from E. coli.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bauernfeind A., Hörl G. Novel R-factor borne beta-lactamase of Escherichia coli confering resistance to cephalosporins. Infection. 1987 Jul-Aug;15(4):257–259. doi: 10.1007/BF01644127. [DOI] [PubMed] [Google Scholar]
- Bobrowski M. M., Matthew M., Barth P. T., Datta N., Grinter N. J., Jacob A. E., Kontomichalou P., Dale J. W., Smith J. T. Plasmid-determined beta-lactamase indistinguishable from the chromosomal beta-lactamase of Escherichia coli. J Bacteriol. 1976 Jan;125(1):149–157. doi: 10.1128/jb.125.1.149-157.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brun-Buisson C., Legrand P., Philippon A., Montravers F., Ansquer M., Duval J. Transferable enzymatic resistance to third-generation cephalosporins during nosocomial outbreak of multiresistant Klebsiella pneumoniae. Lancet. 1987 Aug 8;2(8554):302–306. doi: 10.1016/s0140-6736(87)90891-9. [DOI] [PubMed] [Google Scholar]
- Cuchural G. J., Jr, Tally F. P., Storey J. R., Malamy M. H. Transfer of beta-lactamase-associated cefoxitin resistance in Bacteroides fragilis. Antimicrob Agents Chemother. 1986 May;29(5):918–920. doi: 10.1128/aac.29.5.918. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eley A., Greenwood D. Beta-lactamases of type culture strains of the Bacteroides fragilis group and of strains that hydrolyse cefoxitin, latamoxef and imipenem. J Med Microbiol. 1986 Feb;21(1):49–57. doi: 10.1099/00222615-21-1-49. [DOI] [PubMed] [Google Scholar]
- Eley A., Greenwood D. Characterization of beta-lactamases in clinical isolates of Bacteroides. J Antimicrob Chemother. 1986 Sep;18(3):325–333. doi: 10.1093/jac/18.3.325. [DOI] [PubMed] [Google Scholar]
- Fujii T., Sato K., Miyata K., Inoue M., Mitsuhashi S. Biochemical properties of beta-lactamase produced by Legionella gormanii. Antimicrob Agents Chemother. 1986 May;29(5):925–926. doi: 10.1128/aac.29.5.925. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grace M. E., Gregory F. J., Hung P. P., Fu K. P. Purification and properties of a beta-lactamase from Proteus penneri. J Antibiot (Tokyo) 1986 Jul;39(7):938–942. doi: 10.7164/antibiotics.39.938. [DOI] [PubMed] [Google Scholar]
- Jacoby G. A., Sutton L. beta-Lactamases and beta-lactam resistance in Escherichia coli. Antimicrob Agents Chemother. 1985 Nov;28(5):703–705. doi: 10.1128/aac.28.5.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kliebe C., Nies B. A., Meyer J. F., Tolxdorff-Neutzling R. M., Wiedemann B. Evolution of plasmid-coded resistance to broad-spectrum cephalosporins. Antimicrob Agents Chemother. 1985 Aug;28(2):302–307. doi: 10.1128/aac.28.2.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kontomichalou P. M., Papachristou E. G., Levis G. M. R-mediated beta-lactamases and episomal resistance to the beta-lactam drugs in different bacterial hosts. Antimicrob Agents Chemother. 1974 Jul;6(1):60–72. doi: 10.1128/aac.6.1.60. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Labia R., Morand A., Guionie M., Heitz M., Pitton J. S. Bêtalactamases de Klebsiella oxytoca: étude de leur action sur les céphalosporines de troisième génération. Pathol Biol (Paris) 1986 Jun;34(5 Pt 2):611–615. [PubMed] [Google Scholar]
- Laemmli U. K., Favre M. Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol. 1973 Nov 15;80(4):575–599. doi: 10.1016/0022-2836(73)90198-8. [DOI] [PubMed] [Google Scholar]
- Levesque R., Roy P. H., Letarte R., Pechère J. C. A plasmid-mediated cephalosporinase from Achromobacter species. J Infect Dis. 1982 May;145(5):753–761. doi: 10.1093/infdis/145.2.753. [DOI] [PubMed] [Google Scholar]
- Matsubara N., Yotsuji A., Kumano K., Inoue M., Mitsuhashi S. Purification and some properties of a cephalosporinase from Proteus vulgaris. Antimicrob Agents Chemother. 1981 Jan;19(1):185–187. doi: 10.1128/aac.19.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matthew M. Plasmid-mediated beta-lactamases of Gram-negative bacteria: properties and distribution. J Antimicrob Chemother. 1979 Jul;5(4):349–358. doi: 10.1093/jac/5.4.349. [DOI] [PubMed] [Google Scholar]
- Medeiros A. A., Cohenford M., Jacoby G. A. Five novel plasmid-determined beta-lactamases. Antimicrob Agents Chemother. 1985 May;27(5):715–719. doi: 10.1128/aac.27.5.715. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Medeiros A. A., Hedges R. W., Jacoby G. A. Spread of a "Pseudomonas-specific" beta-lactamase to plasmids of enterobacteria. J Bacteriol. 1982 Feb;149(2):700–707. doi: 10.1128/jb.149.2.700-707.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Minami S., Inoue M., Mitsuhashi S. Purification and properties of cephalosporinase in Escherichia coli. Antimicrob Agents Chemother. 1980 Jul;18(1):77–80. doi: 10.1128/aac.18.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sawai T., Kanno M., Tsukamoto K. Characterization of eight beta-lactamases of Gram-negative bacteria. J Bacteriol. 1982 Nov;152(2):567–571. doi: 10.1128/jb.152.2.567-571.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shigi Y., Matsumoto Y., Kaizu M., Fujishita Y., Kojo H. Mechanism of action of the new orally active cephalosporin FK027. J Antibiot (Tokyo) 1984 Jul;37(7):790–796. doi: 10.7164/antibiotics.37.790. [DOI] [PubMed] [Google Scholar]
- Sirot D., Sirot J., Labia R., Morand A., Courvalin P., Darfeuille-Michaud A., Perroux R., Cluzel R. Transferable resistance to third-generation cephalosporins in clinical isolates of Klebsiella pneumoniae: identification of CTX-1, a novel beta-lactamase. J Antimicrob Chemother. 1987 Sep;20(3):323–334. doi: 10.1093/jac/20.3.323. [DOI] [PubMed] [Google Scholar]
- Yotsuji A., Minami S., Inoue M., Mitsuhashi S. Properties of novel beta-lactamase produced by Bacteroides fragilis. Antimicrob Agents Chemother. 1983 Dec;24(6):925–929. doi: 10.1128/aac.24.6.925. [DOI] [PMC free article] [PubMed] [Google Scholar]
- el Solh N., Allignet J., Bismuth R., Buret B., Fouace J. M. Conjugative transfer of staphylococcal antibiotic resistance markers in the absence of detectable plasmid DNA. Antimicrob Agents Chemother. 1986 Jul;30(1):161–169. doi: 10.1128/aac.30.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]