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. 2002 Oct;36(5):325–329. doi: 10.1136/bjsm.36.5.325

Urine nandrolone metabolites: false positive doping test?

R Kohler 1, M Lambert 1
PMCID: PMC1724543  PMID: 12351328

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Selected References

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  1. Belkien L., Schürmeyer T., Hano R., Gunnarsson P. O., Nieschlag E. Pharmacokinetics of 19-nortestosterone esters in normal men. J Steroid Biochem. 1985 May;22(5):623–629. doi: 10.1016/0022-4731(85)90215-8. [DOI] [PubMed] [Google Scholar]
  2. Berkovitz G. D., Guerami A., Brown T. R., MacDonald P. C., Migeon C. J. Familial gynecomastia with increased extraglandular aromatization of plasma carbon19-steroids. J Clin Invest. 1985 Jun;75(6):1763–1769. doi: 10.1172/JCI111888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Björkhem I., Ek H. Detection and quantitation of 19-norandrosterone in urine by isotope dilution-mass spectrometry. J Steroid Biochem. 1982 Oct;17(4):447–451. doi: 10.1016/0022-4731(82)90640-9. [DOI] [PubMed] [Google Scholar]
  4. Castro-Magana M., Collipp P. J., Chen S. Y., Cheruvanky T., Maddaiah V. T. Zinc nutritional status, androgens, and growth retardation. Am J Dis Child. 1981 Apr;135(4):322–325. doi: 10.1001/archpedi.1981.02130280012005. [DOI] [PubMed] [Google Scholar]
  5. Catlin D. H., Leder B. Z., Ahrens B., Starcevic B., Hatton C. K., Green G. A., Finkelstein J. S. Trace contamination of over-the-counter androstenedione and positive urine test results for a nandrolone metabolite. JAMA. 2000 Nov 22;284(20):2618–2621. doi: 10.1001/jama.284.20.2618. [DOI] [PubMed] [Google Scholar]
  6. Clarkson P. M. Minerals: exercise performance and supplementation in athletes. J Sports Sci. 1991 Summer;9(Spec No):91–116. doi: 10.1080/02640419108729869. [DOI] [PubMed] [Google Scholar]
  7. Cowan D. A., Kicman A. T., Walker C. J., Wheeler M. J. Effect of administration of human chorionic gonadotrophin on criteria used to assess testosterone administration in athletes. J Endocrinol. 1991 Oct;131(1):147–154. doi: 10.1677/joe.0.1310147. [DOI] [PubMed] [Google Scholar]
  8. Cumming D. C., Brunsting L. A., 3rd, Strich G., Ries A. L., Rebar R. W. Reproductive hormone increases in response to acute exercise in men. Med Sci Sports Exerc. 1986 Aug;18(4):369–373. [PubMed] [Google Scholar]
  9. Córdova A., Navas F. J. Effect of training on zinc metabolism: changes in serum and sweat zinc concentrations in sportsmen. Ann Nutr Metab. 1998;42(5):274–282. doi: 10.1159/000012744. [DOI] [PubMed] [Google Scholar]
  10. Debruyckere G., de Sagher R., Van Peteghem C. Clostebol-positive urine after consumption of contaminated meat. Clin Chem. 1992 Sep;38(9):1869–1873. [PubMed] [Google Scholar]
  11. Dehennin L., Bonnaire Y., Plou P. Urinary excretion of 19-norandrosterone of endogenous origin in man: quantitative analysis by gas chromatography-mass spectrometry. J Chromatogr B Biomed Sci Appl. 1999 Jan 22;721(2):301–307. doi: 10.1016/s0378-4347(98)00480-0. [DOI] [PubMed] [Google Scholar]
  12. Dehennin L., Jondet M., Scholler R. Androgen and 19-norsteroid profiles in human preovulatory follicles from stimulated cycles: an isotope dilution-mass spectrometric study. J Steroid Biochem. 1987 Mar;26(3):399–405. doi: 10.1016/0022-4731(87)90107-5. [DOI] [PubMed] [Google Scholar]
  13. Galán Martín A. M., Mariño J. I., García de Tiedra M. P., Marabé J. J., Caballero Loscos M. J., Mariño M. M. Determination of nandrolone and metabolites in urine samples from sedentary persons and sportsmen. J Chromatogr B Biomed Sci Appl. 2001 Sep 25;761(2):229–236. doi: 10.1016/s0378-4347(01)00338-3. [DOI] [PubMed] [Google Scholar]
  14. Gillies H., Derman W. E., Noakes T. D., Smith P., Evans A., Gabriels G. Pseudoephedrine is without ergogenic effects during prolonged exercise. J Appl Physiol (1985) 1996 Dec;81(6):2611–2617. doi: 10.1152/jappl.1996.81.6.2611. [DOI] [PubMed] [Google Scholar]
  15. Haupt H. A., Rovere G. D. Anabolic steroids: a review of the literature. Am J Sports Med. 1984 Nov-Dec;12(6):469–484. doi: 10.1177/036354658401200613. [DOI] [PubMed] [Google Scholar]
  16. Hawley J. A., Dennis S. C., Lindsay F. H., Noakes T. D. Nutritional practices of athletes: are they sub-optimal? J Sports Sci. 1995 Summer;13(Spec No):S75–S81. doi: 10.1080/02640419508732280. [DOI] [PubMed] [Google Scholar]
  17. Hickson R. C., Ball K. L., Falduto M. T. Adverse effects of anabolic steroids. Med Toxicol Adverse Drug Exp. 1989 Jul-Aug;4(4):254–271. doi: 10.1007/BF03259912. [DOI] [PubMed] [Google Scholar]
  18. Horton R., Tait J. F. Androstenedione production and interconversion rates measured in peripheral blood and studies on the possible site of its conversion to testosterone. J Clin Invest. 1966 Mar;45(3):301–313. doi: 10.1172/JCI105344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Khaled S., Brun J. F., Micallel J. P., Bardet L., Cassanas G., Monnier J. F., Orsetti A. Serum zinc and blood rheology in sportsmen (football players). Clin Hemorheol Microcirc. 1997 Jan-Feb;17(1):47–58. [PubMed] [Google Scholar]
  20. Kicman A. T., Brooks R. V. A radioimmunoassay for the metabolites of the anabolic steroid nandrolone. J Pharm Biomed Anal. 1988;6(5):473–483. doi: 10.1016/0731-7085(88)80014-1. [DOI] [PubMed] [Google Scholar]
  21. Kicman A. T., Brooks R. V., Collyer S. C., Cowan D. A., Nanjee M. N., Southan G. J., Wheeler M. J. Criteria to indicate testosterone administration. Br J Sports Med. 1990 Dec;24(4):253–264. doi: 10.1136/bjsm.24.4.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kintz P., Cirimele V., Dumestre-Toulet V., Ludes B. Doping control for nandrolone using hair analysis. J Pharm Biomed Anal. 2001 Mar;24(5-6):1125–1130. doi: 10.1016/s0731-7085(00)00570-7. [DOI] [PubMed] [Google Scholar]
  23. Kintz P., Cirimele V., Ludes B. Norandrosterone et noretiocholanolone: les métabolites révélateurs. Acta Clin Belg. 1999;53 (Suppl 1):68–73. [PubMed] [Google Scholar]
  24. Kintz P. Hair testing and doping control in sport. Toxicol Lett. 1998 Dec 28;102-103:109–113. doi: 10.1016/s0378-4274(98)00294-x. [DOI] [PubMed] [Google Scholar]
  25. Kraemer R. R., Kilgore J. L., Kraemer G. R., Castracane V. D. Growth hormone, IGF-I, and testosterone responses to resistive exercise. Med Sci Sports Exerc. 1992 Dec;24(12):1346–1352. [PubMed] [Google Scholar]
  26. Le Bizec B., Monteau F., Gaudin I., André F. Evidence for the presence of endogenous 19-norandrosterone in human urine. J Chromatogr B Biomed Sci Appl. 1999 Feb 19;723(1-2):157–172. doi: 10.1016/s0378-4347(98)00541-6. [DOI] [PubMed] [Google Scholar]
  27. Le Bizec Bruno, Bryand Fabrice, Gaudin Isabelle, Monteau Fabrice, Poulain Frédéric, Andre François. Endogenous nandrolone metabolites in human urine: preliminary results to discriminate between endogenous and exogenous origin. Steroids. 2002 Feb;67(2):105–110. doi: 10.1016/s0039-128x(01)00139-8. [DOI] [PubMed] [Google Scholar]
  28. Leder B. Z., Longcope C., Catlin D. H., Ahrens B., Schoenfeld D. A., Finkelstein J. S. Oral androstenedione administration and serum testosterone concentrations in young men. JAMA. 2000 Feb 9;283(6):779–782. doi: 10.1001/jama.283.6.779. [DOI] [PubMed] [Google Scholar]
  29. Lewis J. G., Ghanadian R., Chisholm G. D. Serum 5alpha-dihydrotestosterone and testosterone changes with age in man. Acta Endocrinol (Copenh) 1976 Jun;82(2):444–448. doi: 10.1530/acta.0.0820444. [DOI] [PubMed] [Google Scholar]
  30. Longcope C., Kato T., Horton R. Conversion of blood androgens to estrogens in normal adult men and women. J Clin Invest. 1969 Dec;48(12):2191–2201. doi: 10.1172/JCI106185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. MacConnie S. E., Barkan A., Lampman R. M., Schork M. A., Beitins I. Z. Decreased hypothalamic gonadotropin-releasing hormone secretion in male marathon runners. N Engl J Med. 1986 Aug 14;315(7):411–417. doi: 10.1056/NEJM198608143150702. [DOI] [PubMed] [Google Scholar]
  32. Massé R., Laliberté C., Tremblay L., Dugal R. Gas chromatographic/mass spectrometric analysis of 19-nortestosterone urinary metabolites in man. Biomed Mass Spectrom. 1985 Mar;12(3):115–121. doi: 10.1002/bms.1200120305. [DOI] [PubMed] [Google Scholar]
  33. Mathurin J. C., Herrou V., Bourgogne E., Pascaud L., de Ceaurriz J. Gas chromatography-combustion-isotope ratio mass spectrometry analysis of 19-norsteroids: application to the detection of a nandrolone metabolite in urine. J Chromatogr B Biomed Sci Appl. 2001 Aug 15;759(2):267–275. doi: 10.1016/s0378-4347(01)00241-9. [DOI] [PubMed] [Google Scholar]
  34. Morgan W. K. On evidence, embellishment and efficacy. J Eval Clin Pract. 1997 Apr;3(2):117–122. doi: 10.1046/j.1365-2753.1997.00077.x. [DOI] [PubMed] [Google Scholar]
  35. Ozer D., Temìzer A. The determination of nandrolone and its metabolites in urine by gas chromatography-mass spectrometry. Eur J Drug Metab Pharmacokinet. 1997 Oct-Dec;22(4):421–425. doi: 10.1007/BF03190980. [DOI] [PubMed] [Google Scholar]
  36. Pomerantz D. K. Human chorionic gonadotropin enhances the ability of gonadotropic hormones to stimulate aromatization in the testis of the rat. Endocrinology. 1981 Dec;109(6):2004–2008. doi: 10.1210/endo-109-6-2004. [DOI] [PubMed] [Google Scholar]
  37. Prasad A. S., Mantzoros C. S., Beck F. W., Hess J. W., Brewer G. J. Zinc status and serum testosterone levels of healthy adults. Nutrition. 1996 May;12(5):344–348. doi: 10.1016/s0899-9007(96)80058-x. [DOI] [PubMed] [Google Scholar]
  38. Reznik Y., Dehennin L., Coffin C., Mahoudeau J., Leymarie P. Urinary nandrolone metabolites of endogenous origin in man: a confirmation by output regulation under human chorionic gonadotropin stimulation. J Clin Endocrinol Metab. 2001 Jan;86(1):146–150. doi: 10.1210/jcem.86.1.7100. [DOI] [PubMed] [Google Scholar]
  39. Reznik Y., Herrou M., Dehennin L., Lemaire M., Leymarie P. Rising plasma levels of 19-nortestosterone throughout pregnancy: determination by radioimmunoassay and validation by gas chromatography-mass spectrometry. J Clin Endocrinol Metab. 1987 May;64(5):1086–1088. doi: 10.1210/jcem-64-5-1086. [DOI] [PubMed] [Google Scholar]
  40. Robinson N., Taroni F., Saugy M., Ayotte C., Mangin P., Dvorak J. Detection of nandrolone metabolites in urine after a football game in professional and amateur players: a Bayesian comparison. Forensic Sci Int. 2001 Nov 1;122(2-3):130–135. doi: 10.1016/s0379-0738(01)00482-0. [DOI] [PubMed] [Google Scholar]
  41. Shackleton C. H., Phillips A., Chang T., Li Y. Confirming testosterone administration by isotope ratio mass spectrometric analysis of urinary androstanediols. Steroids. 1997 Apr;62(4):379–387. doi: 10.1016/s0039-128x(96)00253-x. [DOI] [PubMed] [Google Scholar]
  42. Shackleton C. H., Roitman E., Phillips A., Chang T. Androstanediol and 5-androstenediol profiling for detecting exogenously administered dihydrotestosterone, epitestosterone, and dehydroepiandrosterone: potential use in gas chromatography isotope ratio mass spectrometry. Steroids. 1997 Oct;62(10):665–673. doi: 10.1016/s0039-128x(97)00065-2. [DOI] [PubMed] [Google Scholar]
  43. Sulcová J., Rafter J., Stárka L. 19-Nortestosterone in mouse kidney. Endocrinol Exp. 1979;13(4):225–235. [PubMed] [Google Scholar]
  44. Sutton J. R., Coleman M. J., Casey J., Lazarus L. Androgen responses during physical exercise. Br Med J. 1973 Mar 3;1(5852):520–522. doi: 10.1136/bmj.1.5852.520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Uralets V. P., Gillette P. A. Over-the-counter anabolic steroids 4-androsten-3,17-dione; 4-androsten-3beta,17beta-diol; and 19-nor-4-androsten-3,17-dione: excretion studies in men. J Anal Toxicol. 1999 Sep;23(5):357–366. doi: 10.1093/jat/23.5.357. [DOI] [PubMed] [Google Scholar]
  46. Uralets V. P., Gillette P. A. Over-the-counter delta5 anabolic steroids 5-androsen-3,17-dione; 5-androsten-3beta, 17beta-diol; dehydroepiandrosterone; and 19-nor-5-androsten-3,17-dione: excretion studies in men. J Anal Toxicol. 2000 Apr;24(3):188–193. doi: 10.1093/jat/24.3.188. [DOI] [PubMed] [Google Scholar]
  47. Van Eenoo P., Delbeke F. T., de Jong F. H., De Backer P. Endogenous origin of norandrosterone in female urine: indirect evidence for the production of 19-norsteroids as by-products in the conversion from androgen to estrogen. J Steroid Biochem Mol Biol. 2001 Oct;78(4):351–357. doi: 10.1016/s0960-0760(01)00112-1. [DOI] [PubMed] [Google Scholar]
  48. Vogel R. B., Books C. A., Ketchum C., Zauner C. W., Murray F. T. Increase of free and total testosterone during submaximal exercise in normal males. Med Sci Sports Exerc. 1985 Feb;17(1):119–123. [PubMed] [Google Scholar]
  49. Wilkerson J. E., Horvath S. M., Gutin B. Plasma testosterone during treadmill exercise. J Appl Physiol Respir Environ Exerc Physiol. 1980 Aug;49(2):249–253. doi: 10.1152/jappl.1980.49.2.249. [DOI] [PubMed] [Google Scholar]

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