Abstract
Background—Cap polyposis is a rare disease characterised by mucoid and bloody diarrhoea, with polyps covered by a cap of mucoid and fibrinopurulent exudate. The pathogenesis is not known. Aims—To pour some light on cap polyposis pathogenesis, by examining the mucus of patients and analysing the expression of five mucin genes, MUC2, MUC3, MUC4, MUC5AC, and MUC5B. Patient and methods—The study was performed on biopsy specimens taken from a patient with recurrent cap polyposis. Histochemical examination, electron microscopy, and mRNA in situ hybridisation were used. Results—The mucus of cap polyposis differed in three respects from that of normal adult colon: abnormal ultrastructure of the mucus in the goblet cells, predominance of non-sulphated mucins, abnormal expression of the MUC4, MUC3, and MUC5AC genes. Conclusions—Most of these abnormalities have been reported for other pathological situations, suggesting that the abnormalities observed in the mucus of this patient with cap polyposis are probably secondary phenomena rather than primary. However, the mucin abnormalities detected, which reflect deregulation of the expression of three apomucin genes, abnormal glycosylation, and abnormalities of the secretion process, are also probably involved in the clinical manifestations of cap polyposis.
Keywords: cap polyposis; mucins; histochemistry; ultrastructure; in situ hybridisation
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Figure 1 .
Recurrence of cap polyposis: endoscopic appearance of the polyps.
Figure 2 .
Histochemical examination using high iron diamine/alcian blue at pH 2.5 of (A) cap polyposis with exclusive expression of non-sulphated mucins and (B) normal colon with predominance of sulphomucins.
Figure 3 .
Transmission electron microscopy of colonic epithelium. (A) Cap polyposis: the thick superficial layer of mucus is in continuity with the mucus of goblet cells, but does not adhere to the cytoplasmic membrane of epithelial cells; mucus droplets coalesce. (B) Normal colon: the mucus is only found within the goblet cells; well formed mucus droplets are found from the bottom to the apex of the goblets.
Figure 4 .
In situ hybridisation with the MUC4 probe using methyl green pyronin counterstain. (A) Cap polyposis: strong labelling of all epithelial cells; (B) normal colon: moderate labelling of all epithelial cells.