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Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1989 Sep;33(9):1624–1626. doi: 10.1128/aac.33.9.1624

Restriction maps of the regions coding for methicillin and tobramycin resistances on chromosomal DNA in methicillin-resistant staphylococci.

K Ubukata 1, R Nonoguchi 1, M Matsuhashi 1, M D Song 1, M Konno 1
PMCID: PMC172717  PMID: 2817861

Abstract

Chromosomal BamHI DNA fragments containing both the mecA gene encoding the penicillin-binding protein responsible for methicillin resistance and the aadD gene encoding 4',4"-adenylyltransferase responsible for tobramycin resistance were cloned from three methicillin- and tobramycin-resistant strains of Staphylococcus aureus and one strain of Staphylococcus epidermidis. Physical maps of the fragments were similar, suggesting their unique origin.

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Selected References

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  1. Hartman B. J., Tomasz A. Expression of methicillin resistance in heterogeneous strains of Staphylococcus aureus. Antimicrob Agents Chemother. 1986 Jan;29(1):85–92. doi: 10.1128/aac.29.1.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hartman B. J., Tomasz A. Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus. J Bacteriol. 1984 May;158(2):513–516. doi: 10.1128/jb.158.2.513-516.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Inglis B., Matthews P. R., Stewart P. R. The expression in Staphylococcus aureus of cloned DNA encoding methicillin resistance. J Gen Microbiol. 1988 Jun;134(6):1465–1469. doi: 10.1099/00221287-134-6-1465. [DOI] [PubMed] [Google Scholar]
  4. Konno M., Ubukata K., Yamashita N., Matsushita M., Kawakami S., Masuda M., Nonoguchi R. [Relationship between resistant pattern to antibiotics and phage type of methicillin-resistant Staphylococcus aureus]. Kansenshogaku Zasshi. 1985 Nov;59(11):1029–1040. doi: 10.11150/kansenshogakuzasshi1970.59.1029. [DOI] [PubMed] [Google Scholar]
  5. Matsuhashi M., Song M. D., Ishino F., Wachi M., Doi M., Inoue M., Ubukata K., Yamashita N., Konno M. Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to beta-lactam antibiotics in Staphylococcus aureus. J Bacteriol. 1986 Sep;167(3):975–980. doi: 10.1128/jb.167.3.975-980.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Matsumura M., Katakura Y., Imanaka T., Aiba S. Enzymatic and nucleotide sequence studies of a kanamycin-inactivating enzyme encoded by a plasmid from thermophilic bacilli in comparison with that encoded by plasmid pUB110. J Bacteriol. 1984 Oct;160(1):413–420. doi: 10.1128/jb.160.1.413-420.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rossi L., Tonin E., Cheng Y. R., Fontana R. Regulation of penicillin-binding protein activity: description of a methicillin-inducible penicillin-binding protein in Staphylococcus aureus. Antimicrob Agents Chemother. 1985 May;27(5):828–831. doi: 10.1128/aac.27.5.828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Song M. D., Wachi M., Doi M., Ishino F., Matsuhashi M. Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion. FEBS Lett. 1987 Aug 31;221(1):167–171. doi: 10.1016/0014-5793(87)80373-3. [DOI] [PubMed] [Google Scholar]
  9. Stewart G. C., Rosenblum E. D. Genetic behavior of the methicillin resistance determinant in Staphylococcus aureus. J Bacteriol. 1980 Dec;144(3):1200–1202. doi: 10.1128/jb.144.3.1200-1202.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tesch W., Strässle A., Berger-Bächi B., O'Hara D., Reynolds P., Kayser F. H. Cloning and expression of methicillin resistance from Staphylococcus epidermidis in Staphylococcus carnosus. Antimicrob Agents Chemother. 1988 Oct;32(10):1494–1499. doi: 10.1128/aac.32.10.1494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Trees D. L., Iandolo J. J. Identification of a Staphylococcus aureus transposon (Tn4291) that carries the methicillin resistance gene(s). J Bacteriol. 1988 Jan;170(1):149–154. doi: 10.1128/jb.170.1.149-154.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ubukata K., Nonoguchi R., Matsuhashi M., Konno M. Expression and inducibility in Staphylococcus aureus of the mecA gene, which encodes a methicillin-resistant S. aureus-specific penicillin-binding protein. J Bacteriol. 1989 May;171(5):2882–2885. doi: 10.1128/jb.171.5.2882-2885.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ubukata K., Yamashita N., Gotoh A., Konno M. Purification and characterization of aminoglycoside-modifying enzymes from Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob Agents Chemother. 1984 Jun;25(6):754–759. doi: 10.1128/aac.25.6.754. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ubukata K., Yamashita N., Konno M. Occurrence of a beta-lactam-inducible penicillin-binding protein in methicillin-resistant staphylococci. Antimicrob Agents Chemother. 1985 May;27(5):851–857. doi: 10.1128/aac.27.5.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Utsui Y., Yokota T. Role of an altered penicillin-binding protein in methicillin- and cephem-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 1985 Sep;28(3):397–403. doi: 10.1128/aac.28.3.397. [DOI] [PMC free article] [PubMed] [Google Scholar]

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