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. 1989 Dec;33(12):2092–2095. doi: 10.1128/aac.33.12.2092

Antibodies to nystatin demonstrate polyene sterol specificity and allow immunolabeling of sterols in Saccharomyces cerevisiae.

H M Walker-Caprioglio 1, J M MacKenzie 1, L W Parks 1
PMCID: PMC172827  PMID: 2694950

Abstract

Polyclonal antibodies elicited by injection into rabbits of a nystatin-bovine serum albumin conjugate were reactive with both nystatin and amphotericin B. Upon labeling of polyene-treated Saccharomyces cerevisiae sterol auxotrophs grown on various sterols, nystatin reacted specifically with ergosterol, while amphotericin B did not react preferentially with ergosterol, cholesterol, or cholestanol. Time course labeling experiments demonstrated the rate of ergosterol transport into cholesterol-grown cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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