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. 1995 Jan;8(1):1–21. doi: 10.1128/cmr.8.1.1

Laboratory diagnosis and interpretation of tests for syphilis.

S A Larsen 1, B M Steiner 1, A H Rudolph 1
PMCID: PMC172846  PMID: 7704889

Abstract

The lack of a method for demonstrating the presence of Treponema pallidum by growth necessitates the use of alternative methods. Traditionally, these methods are divided into direct detection methods (animal inoculation, dark-field microscopy, etc.) and serologic tests for the presence of patient antibody against T. pallidum. Serologic methods are further divided into two classes. One class, the nontreponemal tests, detects antibodies to lipoidal antigens present in either the host or T. pallidum; examples are the Venereal Disease Research Laboratory and rapid plasma reagin and tests. Reactivity in these tests generally indicates host tissue damage that may not be specific for syphilis. Because these tests are easy and inexpensive to perform, they are commonly used for screening, and with proper clinical signs they are suggestive of syphilis. The other class of test, the treponemal tests, uses specific treponemal antigens. Confirmation of infection requires a reactive treponemal test. Examples of the treponemal tests are the microhemagglutination assay for antibodies to T. pallidum and the fluorescent treponemal antibody absorption test. These tests are more expensive and complicated to perform than the nontreponemal tests. On the horizon are a number of direct antigen, enzyme-linked immunosorbent assay, and PCR techniques. Several of these techniques have shown promise in clinical trials for the diagnosis of congenital syphilis and neurosyphilis that are presently difficult to diagnose.

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Selected References

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  1. ANDUJAR J. J., MAZUREK E. E. The plasmacrit (PCT) test on capillary blood; unheated plasma screen test for syphilis. Am J Clin Pathol. 1959 Mar;31(3):197–204. doi: 10.1093/ajcp/31.3.197. [DOI] [PubMed] [Google Scholar]
  2. Alford C. A., Jr, Polt S. S., Cassady G. E., Straumfjord J. V., Remington J. S. Gamma-M-fluorescent treponemal antibody in the diagnosis of congenital syphilis. N Engl J Med. 1969 May 15;280(20):1086–1091. doi: 10.1056/NEJM196905152802002. [DOI] [PubMed] [Google Scholar]
  3. Anderson B., Stillman M. T. False-positive FTA-ABS in hydralazine-induced lupus. JAMA. 1978 Apr 3;239(14):1392–1393. doi: 10.1001/jama.1978.03280410034004. [DOI] [PubMed] [Google Scholar]
  4. Antal G. M., Causse G. The control of endemic treponematoses. Rev Infect Dis. 1985 May-Jun;7 (Suppl 2):S220–S226. doi: 10.1093/clinids/7-supplement_2.s220. [DOI] [PubMed] [Google Scholar]
  5. Berry C. D., Hooton T. M., Collier A. C., Lukehart S. A. Neurologic relapse after benzathine penicillin therapy for secondary syphilis in a patient with HIV infection. N Engl J Med. 1987 Jun 18;316(25):1587–1589. doi: 10.1056/NEJM198706183162507. [DOI] [PubMed] [Google Scholar]
  6. Bromberg K., Rawstron S., Tannis G. Diagnosis of congenital syphilis by combining Treponema pallidum-specific IgM detection with immunofluorescent antigen detection for T. pallidum. J Infect Dis. 1993 Jul;168(1):238–242. doi: 10.1093/infdis/168.1.238. [DOI] [PubMed] [Google Scholar]
  7. Brown S. T., Zaidi A., Larsen S. A., Reynolds G. H. Serological response to syphilis treatment. A new analysis of old data. JAMA. 1985 Mar 1;253(9):1296–1299. [PubMed] [Google Scholar]
  8. Buchanan C. S., Haserick J. R. FTA-ABS test in pregnancy. A probable false-positive reaction. Arch Dermatol. 1970 Sep;102(3):322–325. [PubMed] [Google Scholar]
  9. Buist D. G., Pertile R., Morris G. J. Evaluation of the Treponema pallidum haemagglutination test. Pathology. 1973 Jul;5(3):249–252. doi: 10.3109/00313027309060842. [DOI] [PubMed] [Google Scholar]
  10. Burdash N. M., Hinds K. K., Finnerty J. A., Manos J. P. Evaluation of the syphilis Bio-EnzaBead assay for detection of treponemal antibody. J Clin Microbiol. 1987 May;25(5):808–811. doi: 10.1128/jcm.25.5.808-811.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Burstain J. M., Grimprel E., Lukehart S. A., Norgard M. V., Radolf J. D. Sensitive detection of Treponema pallidum by using the polymerase chain reaction. J Clin Microbiol. 1991 Jan;29(1):62–69. doi: 10.1128/jcm.29.1.62-69.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Byrne R. E., Laska S., Bell M., Larson D., Phillips J., Todd J. Evaluation of a Treponema pallidum western immunoblot assay as a confirmatory test for syphilis. J Clin Microbiol. 1992 Jan;30(1):115–122. doi: 10.1128/jcm.30.1.115-122.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. CUTLER J. C., BAUER T. J., PRICE E. V., SCHWIMMER B. H. Comparison of spinal fluid findings among syphilitic and nonsyphilitic individuals. Am J Syph Gonorrhea Vener Dis. 1954 Sep;38(5):447–458. [PubMed] [Google Scholar]
  14. Castro K. G., Lieb S., Jaffe H. W., Narkunas J. P., Calisher C. H., Bush T. J., Witte J. J. Transmission of HIV in Belle Glade, Florida: lessons for other communities in the United States. Science. 1988 Jan 8;239(4836):193–197. doi: 10.1126/science.3336781. [DOI] [PubMed] [Google Scholar]
  15. Catterall R. D. Presidential Address to the M.S.S.V.D. Systemic disease and the biological false positive reaction. Br J Vener Dis. 1972 Feb;48(1):1–12. doi: 10.1136/sti.48.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Chhabra R. S., Brion L. P., Castro M., Freundlich L., Glaser J. H. Comparison of maternal sera, cord blood, and neonatal sera for detecting presumptive congenital syphilis: relationship with maternal treatment. Pediatrics. 1993 Jan;91(1):88–91. [PubMed] [Google Scholar]
  17. Cox P. M., Logan L. C., Norins L. C. Automated, quantitative microhemagglutination assay for Treponema pallidum antibodies. Appl Microbiol. 1969 Sep;18(3):485–489. doi: 10.1128/am.18.3.485-489.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. D'ALESSANDRO G., DARDANONI L. Isolation and purification of the protein antigen of the Reiter treponeme; a study of its serologic reactions. Am J Syph Gonorrhea Vener Dis. 1953 Mar;37(2):137–150. [PubMed] [Google Scholar]
  19. DATTNER B., THOMAS E. W., DE MELLO L. Criteria for the management of neurosyphilis. Am J Med. 1951 Apr;10(4):463–467. doi: 10.1016/0002-9343(51)90292-6. [DOI] [PubMed] [Google Scholar]
  20. DEACON W. E., FALCONE V. H., HARRIS A. A fluorescent test for treponemal antibodies. Proc Soc Exp Biol Med. 1957 Nov;96(2):477–480. doi: 10.3181/00379727-96-23512. [DOI] [PubMed] [Google Scholar]
  21. DEACON W. E., FREEMAN E. M., HARRIS A. Fluorescent treponemal antibody test. Modification based on quantitation (FTA-200). Proc Soc Exp Biol Med. 1960 Apr;103:827–829. doi: 10.3181/00379727-103-25685. [DOI] [PubMed] [Google Scholar]
  22. DEACON W. E., HUNTER E. F. Treponemal antigens as related to identification and syphilis serology. Proc Soc Exp Biol Med. 1962 Jun;110:352–356. doi: 10.3181/00379727-110-27515. [DOI] [PubMed] [Google Scholar]
  23. Daniels K. C., Ferneyhough H. S. Specific direct fluorescent antibody detection of Treponema pallidum. Health Lab Sci. 1977 Jul;14(3):164–171. [PubMed] [Google Scholar]
  24. Dawson S., Evans B. A., Lawrence A. G. Benign tertiary syphilis and HIV infection. AIDS. 1988 Aug;2(4):315–316. doi: 10.1097/00002030-198808000-00012. [DOI] [PubMed] [Google Scholar]
  25. Dobson S. R., Taber L. H., Baughn R. E. Recognition of Treponema pallidum antigens by IgM and IgG antibodies in congenitally infected newborns and their mothers. J Infect Dis. 1988 May;157(5):903–910. doi: 10.1093/infdis/157.5.903. [DOI] [PubMed] [Google Scholar]
  26. Duncan W. P., Jenkins T. W., Parham C. E. Fluorescent treponemal antibody-cerebrospinal fluid (FTA-CSF) test. A provisional technique. Br J Vener Dis. 1972 Apr;48(2):97–101. doi: 10.1136/sti.48.2.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Engelkens H. J., Judanarso J., Oranje A. P., Vuzevski V. D., Niemel P. L., van der Sluis J. J., Stolz E. Endemic treponematoses. Part I. Yaws. Int J Dermatol. 1991 Feb;30(2):77–83. doi: 10.1111/j.1365-4362.1991.tb04215.x. [DOI] [PubMed] [Google Scholar]
  28. Engelkens H. J., Niemel P. L., van der Sluis J. J., Meheus A., Stolz E. Endemic treponematoses. Part II. Pinta and endemic syphilis. Int J Dermatol. 1991 Apr;30(4):231–238. doi: 10.1111/j.1365-4362.1991.tb04626.x. [DOI] [PubMed] [Google Scholar]
  29. Escobar M. R., Dalton H. P., Allison M. J. Fluorescent antibody tests for syphilis using cerebrospinal fluid: clinical correlation in 150 cases. Am J Clin Pathol. 1970 Jun;53(6):886–890. doi: 10.1093/ajcp/53.6.886. [DOI] [PubMed] [Google Scholar]
  30. FALCONE V. H., STOUT G. W., MOORE M. B., Jr EVALUATION OF RAPID PLASMA REAGIN (CIRCLE) CARD TEST. Public Health Rep. 1964 Jun;79:491–495. [PMC free article] [PubMed] [Google Scholar]
  31. Farshy C. E., Hunter E. F., Helsel L. O., Larsen S. A. Four-step enzyme-linked immunosorbent assay for detection of Treponema pallidum antibody. J Clin Microbiol. 1985 Mar;21(3):387–389. doi: 10.1128/jcm.21.3.387-389.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Fernández-Guerrero M. L., Miranda C., Cenjor C., Sanabria F. The treatment of neurosyphilis in patients with HIV infection. JAMA. 1988 Mar 11;259(10):1495–1496. doi: 10.1001/jama.1988.03720100017015. [DOI] [PubMed] [Google Scholar]
  33. Fieldsteel A. H., Cox D. L., Moeckli R. A. Cultivation of virulent Treponema pallidum in tissue culture. Infect Immun. 1981 May;32(2):908–915. doi: 10.1128/iai.32.2.908-915.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Fitzgerald T. J. Pathogenesis and immunology of Treponema pallidum. Annu Rev Microbiol. 1981;35:29–54. doi: 10.1146/annurev.mi.35.100181.000333. [DOI] [PubMed] [Google Scholar]
  35. Fiumara N. J. Reinfection primary, secondary, and latent syphilis: the serologic response after treatment. Sex Transm Dis. 1980 Jul-Sep;7(3):111–115. doi: 10.1097/00007435-198007000-00003. [DOI] [PubMed] [Google Scholar]
  36. Fiumara N. J. Serologic responses to treatment of 128 patients with late latent syphilis. Sex Transm Dis. 1979 Oct-Dec;6(4):243–246. doi: 10.1097/00007435-197910000-00003. [DOI] [PubMed] [Google Scholar]
  37. Fiumara N. J. Treatment of early latent syphilis of less than a year's duration: an evaluation of 275 cases. Sex Transm Dis. 1978 Jul-Sep;5(3):85–88. [PubMed] [Google Scholar]
  38. Fiumara N. J. Treatment of primary and secondary syphilis. Serological response. JAMA. 1980 Jun 27;243(24):2500–2502. [PubMed] [Google Scholar]
  39. Fohn M. J., Wignall S., Baker-Zander S. A., Lukehart S. A. Specificity of antibodies from patients with pinta for antigens of Treponema pallidum subspecies pallidum. J Infect Dis. 1988 Jan;157(1):32–37. doi: 10.1093/infdis/157.1.32. [DOI] [PubMed] [Google Scholar]
  40. Garner M. F., Backhouse J. L. Fluorescent treponemal antibody tests on cerebrospinal fluid. Br J Vener Dis. 1971 Oct;47(5):356–358. doi: 10.1136/sti.47.5.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Gershman K. A., Rolfs R. T., Larsen S. A., Zaidi A., Palafox N. A. Seroepidemiological characterization of a syphilis epidemic in the Republic of the Marshall Islands, formerly a yaws endemic area. Int J Epidemiol. 1992 Jun;21(3):599–606. doi: 10.1093/ije/21.3.599. [DOI] [PubMed] [Google Scholar]
  42. Goldman J. N., Lantz M. A. FTA-ABS and VDRL slide test reactivity in a population of nuns. JAMA. 1971 Jul 5;217(1):53–55. [PubMed] [Google Scholar]
  43. Gourevitch M. N., Selwyn P. A., Davenny K., Buono D., Schoenbaum E. E., Klein R. S., Friedland G. H. Effects of HIV infection on the serologic manifestations and response to treatment of syphilis in intravenous drug users. Ann Intern Med. 1993 Mar 1;118(5):350–355. doi: 10.7326/0003-4819-118-5-199303010-00005. [DOI] [PubMed] [Google Scholar]
  44. Gregory N., Sanchez M., Buchness M. R. The spectrum of syphilis in patients with human immunodeficiency virus infection. J Am Acad Dermatol. 1990 Jun;22(6 Pt 1):1061–1067. doi: 10.1016/0190-9622(90)70153-9. [DOI] [PubMed] [Google Scholar]
  45. Grimprel E., Sanchez P. J., Wendel G. D., Burstain J. M., McCracken G. H., Jr, Radolf J. D., Norgard M. V. Use of polymerase chain reaction and rabbit infectivity testing to detect Treponema pallidum in amniotic fluid, fetal and neonatal sera, and cerebrospinal fluid. J Clin Microbiol. 1991 Aug;29(8):1711–1718. doi: 10.1128/jcm.29.8.1711-1718.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Grossman L. J., Peery T. M. Biologically false-positive serologic tests for syphilis due to smallpox vaccination. Am J Clin Pathol. 1969 Mar;51(3):375–378. doi: 10.1093/ajcp/51.3.375. [DOI] [PubMed] [Google Scholar]
  47. HARRIS A., BOSSAK H. M., DEACON W. E., BUNCH W. L., Jr Comparison of the fluorescent treponemal antibody test with other tests for syphilis on cerebrospinal fluids. Br J Vener Dis. 1960 Sep;36:178–180. doi: 10.1136/sti.36.3.178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. HUNTER E. F., DEACON W. E., MEYER P. E. AN IMPROVED FTA TEST FOR SYPHILIS, THE ABSORPTION PROCEDURE (FTA-ABS). Public Health Rep. 1964 May;79:410–412. [PMC free article] [PubMed] [Google Scholar]
  49. Haas J. S., Bolan G., Larsen S. A., Clement M. J., Bacchetti P., Moss A. R. Sensitivity of treponemal tests for detecting prior treated syphilis during human immunodeficiency virus infection. J Infect Dis. 1990 Oct;162(4):862–866. doi: 10.1093/infdis/162.4.862. [DOI] [PubMed] [Google Scholar]
  50. Hay P. E., Clarke J. R., Strugnell R. A., Taylor-Robinson D., Goldmeier D. Use of the polymerase chain reaction to detect DNA sequences specific to pathogenic treponemes in cerebrospinal fluid. FEMS Microbiol Lett. 1990 Mar 15;56(3):233–238. doi: 10.1111/j.1574-6968.1990.tb13943.x. [DOI] [PubMed] [Google Scholar]
  51. Hederstedt B. Studies on the Treponema pallidum immobilizing activity in normal human serum. 2. Serum factors participating in the normal immobilization reaction. Acta Pathol Microbiol Scand C. 1976 Apr;84(2):135–141. doi: 10.1111/j.1699-0463.1976.tb00011.x. [DOI] [PubMed] [Google Scholar]
  52. Hicks C. B., Benson P. M., Lupton G. P., Tramont E. C. Seronegative secondary syphilis in a patient infected with the human immunodeficiency virus (HIV) with Kaposi sarcoma. A diagnostic dilemma. Ann Intern Med. 1987 Oct;107(4):492–495. doi: 10.7326/0003-4819-107-4-492. [DOI] [PubMed] [Google Scholar]
  53. Holodniy M., Kim S., Katzenstein D., Konrad M., Groves E., Merigan T. C. Inhibition of human immunodeficiency virus gene amplification by heparin. J Clin Microbiol. 1991 Apr;29(4):676–679. doi: 10.1128/jcm.29.4.676-679.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  54. Hook E. W., 3rd, Roddy R. E., Lukehart S. A., Hom J., Holmes K. K., Tam M. R. Detection of Treponema pallidum in lesion exudate with a pathogen-specific monoclonal antibody. J Clin Microbiol. 1985 Aug;22(2):241–244. doi: 10.1128/jcm.22.2.241-244.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  55. Hunter E. F., Greer P. W., Swisher B. L., Simons A. R., Farshy C. E., Crawford J. A., Sulzer K. R. Immunofluorescent staining of Treponema in tissues fixed with formalin. Arch Pathol Lab Med. 1984 Nov;108(11):878–880. [PubMed] [Google Scholar]
  56. Hunter E. F., McKinney R. M., Maddison S. E., Cruce D. D. Double-staining procedure for the fluorescent treponemal antibody absorption (FTA-ABS) test. Br J Vener Dis. 1979 Apr;55(2):105–108. doi: 10.1136/sti.55.2.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  57. Hunter E. F., Russell H., Farshy C. E., Sampson J. S., Larsen S. A. Evaluation of sera from patients with Lyme disease in the fluorescent treponemal antibody-absorption test for syphilis. Sex Transm Dis. 1986 Oct-Dec;13(4):232–236. doi: 10.1097/00007435-198610000-00005. [DOI] [PubMed] [Google Scholar]
  58. Ijsselmuiden O. E., van der Sluis J. J., Mulder A., Stolz E., Bolton K. P., van Eijk R. V. An IgM capture enzyme linked immunosorbent assay to detect IgM antibodies to treponemes in patients with syphilis. Genitourin Med. 1989 Apr;65(2):79–83. doi: 10.1136/sti.65.2.79. [DOI] [PMC free article] [PubMed] [Google Scholar]
  59. Ito F., Hunter E. F., George R. W., Pope V., Larsen S. A. Specific immunofluorescent staining of pathogenic treponemes with a monoclonal antibody. J Clin Microbiol. 1992 Apr;30(4):831–838. doi: 10.1128/jcm.30.4.831-838.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  60. Ito F., Hunter E. F., George R. W., Swisher B. L., Larsen S. A. Specific immunofluorescence staining of Treponema pallidum in smears and tissues. J Clin Microbiol. 1991 Mar;29(3):444–448. doi: 10.1128/jcm.29.3.444-448.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  61. Jaffe H. W., Larsen S. A., Jones O. G., Dans P. E. Hemagglutination tests for syphilis antibody. Am J Clin Pathol. 1978 Aug;70(2):230–233. doi: 10.1093/ajcp/70.2.230. [DOI] [PubMed] [Google Scholar]
  62. Jaffe H. W., Larsen S. A., Peters M., Jove D. F., Lopez B., Schroeter A. L. Tests for treponemal antibody in CSF. Arch Intern Med. 1978 Feb;138(2):252–255. [PubMed] [Google Scholar]
  63. Jensen J. R., From E. Alterations in T lymphocytes and T-lymphocyte subpopulations in patients with syphilis. Br J Vener Dis. 1982 Feb;58(1):18–22. doi: 10.1136/sti.58.1.18. [DOI] [PMC free article] [PubMed] [Google Scholar]
  64. Johns D. R., Tierney M., Felsenstein D. Alteration in the natural history of neurosyphilis by concurrent infection with the human immunodeficiency virus. N Engl J Med. 1987 Jun 18;316(25):1569–1572. doi: 10.1056/NEJM198706183162503. [DOI] [PubMed] [Google Scholar]
  65. Johnston N. A. Neonatal congenital syphilis. Diagnosis by the absorbed fluorescent treponemal antibody (IgM) test. Br J Vener Dis. 1972 Dec;48(6):464–469. doi: 10.1136/sti.48.6.464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  66. Johnston N. A. Treponema pallidum haemagglutination test for syphilis. Evaluation of a modified micro-method. Br J Vener Dis. 1972 Dec;48(6):474–478. doi: 10.1136/sti.48.6.474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  67. Jones S. A., Marchitto K. S., Miller J. N., Norgard M. V. Monoclonal antibody with hemagglutination, immobilization, and neutralization activities defines an immunodominant, 47,000 mol wt, surface-exposed immunogen of Treponema pallidum (Nichols). J Exp Med. 1984 Nov 1;160(5):1404–1420. doi: 10.1084/jem.160.5.1404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  68. Jurado R. L., Campbell J., Martin P. D. Prozone phenomenon in secondary syphilis. Has its time arrived? Arch Intern Med. 1993 Nov 8;153(21):2496–2498. [PubMed] [Google Scholar]
  69. Kampmeier R. H. The evolution of the flocculation screening test for syphilis. Sex Transm Dis. 1983 Jul-Sep;10(3):156–159. doi: 10.1097/00007435-198307000-00014. [DOI] [PubMed] [Google Scholar]
  70. Kase C. S., Levitz S. M., Wolinsky J. S., Sulis C. A. Pontine pure motor hemiparesis due to meningovascular syphilis in human immunodeficiency virus-positive patients. Arch Neurol. 1988 Aug;45(8):832–832. doi: 10.1001/archneur.1988.00520320018007. [DOI] [PubMed] [Google Scholar]
  71. Kaufman R. E., Jones O. G., Blount J. H., Wiesner P. J. Questionnaire survey of reported early congenital syphilis: problems in diagnosis, prevention, and treatment. Sex Transm Dis. 1977 Oct-Dec;4(4):135–139. doi: 10.1097/00007435-197710000-00003. [DOI] [PubMed] [Google Scholar]
  72. Kellogg D. S., Jr, Mothershed S. M. Immunofluorescent detection of Treponema pallidum: a review. JAMA. 1969 Feb 3;207(5):938–941. [PubMed] [Google Scholar]
  73. Kellogg D. S., Jr The detection of Treponema Pallidum by a rapid, direct fluorescent antibody darkfield (DFATP) procedure. Health Lab Sci. 1970 Jan;7(1):34–41. [PubMed] [Google Scholar]
  74. Kraus S. J., Haserick J. R., Lantz M. A. Fluorescent treponemal antibody-absorption test reactions in lupus erythematosus. N Engl J Med. 1970 Jun 4;282(23):1287–1290. doi: 10.1056/NEJM197006042822303. [DOI] [PubMed] [Google Scholar]
  75. Kraus S. J., Haserick J. R., Logan L. C., Bullard J. C. Atypical fluorescence in the fluorescent treponemal-antibody-absorption (FTA-ABS) test related to deoxyribonucleic acid (DNA) antibodies. J Immunol. 1971 Jun;106(6):1665–1669. [PubMed] [Google Scholar]
  76. Kwok S., Higuchi R. Avoiding false positives with PCR. Nature. 1989 May 18;339(6221):237–238. doi: 10.1038/339237a0. [DOI] [PubMed] [Google Scholar]
  77. Lane H. C., Masur H., Edgar L. C., Whalen G., Rook A. H., Fauci A. S. Abnormalities of B-cell activation and immunoregulation in patients with the acquired immunodeficiency syndrome. N Engl J Med. 1983 Aug 25;309(8):453–458. doi: 10.1056/NEJM198308253090803. [DOI] [PubMed] [Google Scholar]
  78. Lanska M. J., Lanska D. J., Schmidley J. W. Syphilitic polyradiculopathy in an HIV-positive man. Neurology. 1988 Aug;38(8):1297–1301. doi: 10.1212/wnl.38.8.1297. [DOI] [PubMed] [Google Scholar]
  79. Larsen S. A., Hambie E. A., Pettit D. E., Perryman M. W., Kraus S. J. Specificity, sensitivity, and reproducibility among the fluorescent treponemal antibody-absorption test, the microhemagglutination assay for Treponema pallidum antibodies, and the hemagglutination treponemal test for syphilis. J Clin Microbiol. 1981 Oct;14(4):441–445. doi: 10.1128/jcm.14.4.441-445.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  80. Larsen S. A., Oberle M. W., Sanchez-Braverman J. M., Rosero-Bixby L., Vetter K. M. A population-based serosurveillance of syphilis in Costa Rica. Sex Transm Dis. 1991 Apr-Jun;18(2):124–128. doi: 10.1097/00007435-199118020-00013. [DOI] [PubMed] [Google Scholar]
  81. Lefevre J. C., Bertrand M. A., Bauriaud R. Evaluation of the Captia enzyme immunoassays for detection of immunoglobulins G and M to Treponema pallidum in syphilis. J Clin Microbiol. 1990 Aug;28(8):1704–1707. doi: 10.1128/jcm.28.8.1704-1707.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  82. Lewis L. L., Taber L. H., Baughn R. E. Evaluation of immunoglobulin M western blot analysis in the diagnosis of congenital syphilis. J Clin Microbiol. 1990 Feb;28(2):296–302. doi: 10.1128/jcm.28.2.296-302.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  83. Lukehart S. A., Hook E. W., 3rd, Baker-Zander S. A., Collier A. C., Critchlow C. W., Handsfield H. H. Invasion of the central nervous system by Treponema pallidum: implications for diagnosis and treatment. Ann Intern Med. 1988 Dec 1;109(11):855–862. doi: 10.7326/0003-4819-109-11-855. [DOI] [PubMed] [Google Scholar]
  84. MAGNUSON H. J., THOMAS E. W., OLANSKY S., KAPLAN B. I., DE MELLO L., CUTLER J. C. Inoculation syphilis in human volunteers. Medicine (Baltimore) 1956 Feb;35(1):33–82. doi: 10.1097/00005792-195602000-00002. [DOI] [PubMed] [Google Scholar]
  85. MAHONEY J. F., ARNOLD R. C., HARRIS A. Penicillin treatment of early syphilis; first four patients after 6 years. J Vener Dis Inf. 1949 Dec;30(12):350–355. [PubMed] [Google Scholar]
  86. Magnarelli L. A., Miller J. N., Anderson J. F., Riviere G. R. Cross-reactivity of nonspecific treponemal antibody in serologic tests for Lyme disease. J Clin Microbiol. 1990 Jun;28(6):1276–1279. doi: 10.1128/jcm.28.6.1276-1279.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  87. Mamunes P., Cave V. G., Budell J. W., Andersen J. A., Steward R. E. Early diagnosis of neonatal syphilis. Evaluation of a gamma M-fluorescent treponemal antibody test. Am J Dis Child. 1970 Jul;120(1):17–21. doi: 10.1001/archpedi.1970.02100060051005. [DOI] [PubMed] [Google Scholar]
  88. March R. W., Stiles G. E. The reagin screen test: a new reagin card test for syphilis. Sex Transm Dis. 1980 Apr-Jun;7(2):66–70. doi: 10.1097/00007435-198004000-00006. [DOI] [PubMed] [Google Scholar]
  89. Matthews H. M., Yang T. K., Jenkin H. M. Unique lipid composition of Treponema pallidum (Nichols virulent strain). Infect Immun. 1979 Jun;24(3):713–719. doi: 10.1128/iai.24.3.713-719.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  90. McCabe E., Jaffe L. R., Diaz A. Human immunodeficiency virus seropositivity in adolescents with syphilis. Pediatrics. 1993 Nov;92(5):695–698. [PubMed] [Google Scholar]
  91. McGrew B. E., DuCros M. J., Stout G. W., Falcone V. H. Automation of a flocculation test for syphilis. Am J Clin Pathol. 1968 Jul;50(1):52–59. doi: 10.1093/ajcp/50.1.52. [DOI] [PubMed] [Google Scholar]
  92. McKenna C. H., Schroeter A. L., Kierland R. R., Stilwell G. G., Pien F. D. The fluorescent treponemal antibody absorbed (FTA-ABS) test beading phenomenon in connective tissue diseases. Mayo Clin Proc. 1973 Aug;48(8):545–548. [PubMed] [Google Scholar]
  93. Meheus A., Antal G. M. The endemic treponematoses: not yet eradicated. World Health Stat Q. 1992;45(2-3):228–237. [PubMed] [Google Scholar]
  94. Meyer M. P., Eddy T., Baughn R. E. Analysis of western blotting (immunoblotting) technique in diagnosis of congenital syphilis. J Clin Microbiol. 1994 Mar;32(3):629–633. doi: 10.1128/jcm.32.3.629-633.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  95. Monson R. A. Biologic false-positive FTA-ABS test in drug-induced lupus erythematosus. JAMA. 1973 May 14;224(7):1028–1030. [PubMed] [Google Scholar]
  96. Moyer N. P., Hudson J. D., Hausler W. J., Jr Evaluation of the Bio-EnzaBead test for syphilis. J Clin Microbiol. 1987 Apr;25(4):619–623. doi: 10.1128/jcm.25.4.619-623.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  97. Musher D. M. Syphilis, neurosyphilis, penicillin, and AIDS. J Infect Dis. 1991 Jun;163(6):1201–1206. doi: 10.1093/infdis/163.6.1201. [DOI] [PubMed] [Google Scholar]
  98. Müller F. Specific immunoglobulin M and G antibodies in the rapid diagnosis of human treponemal infections. Diagn Immunol. 1986;4(1):1–9. [PubMed] [Google Scholar]
  99. Nayar R., Campos J. M. Evaluation of the DCL Syphilis-G enzyme immunoassay test kit for the serologic diagnosis of syphilis. Am J Clin Pathol. 1993 Mar;99(3):282–285. doi: 10.1093/ajcp/99.3.282. [DOI] [PubMed] [Google Scholar]
  100. Noordhoek G. T., Engelkens H. J., Judanarso J., van der Stek J., Aelbers G. N., van der Sluis J. J., van Embden J. D., Stolz E. Yaws in West Sumatra, Indonesia: clinical manifestations, serological findings and characterisation of new Treponema isolates by DNA probes. Eur J Clin Microbiol Infect Dis. 1991 Jan;10(1):12–19. doi: 10.1007/BF01967091. [DOI] [PubMed] [Google Scholar]
  101. Noordhoek G. T., Hermans P. W., Paul A. N., Schouls L. M., van der Sluis J. J., van Embden J. D. Treponema pallidum subspecies pallidum (Nichols) and Treponema pallidum subspecies pertenue (CDC 2575) differ in at least one nucleotide: comparison of two homologous antigens. Microb Pathog. 1989 Jan;6(1):29–42. doi: 10.1016/0882-4010(89)90005-3. [DOI] [PubMed] [Google Scholar]
  102. Noordhoek G. T., Wolters E. C., de Jonge M. E., van Embden J. D. Detection by polymerase chain reaction of Treponema pallidum DNA in cerebrospinal fluid from neurosyphilis patients before and after antibiotic treatment. J Clin Microbiol. 1991 Sep;29(9):1976–1984. doi: 10.1128/jcm.29.9.1976-1984.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  103. Norris S. J. In vitro cultivation of Treponema pallidum: independent confirmation. Infect Immun. 1982 Apr;36(1):437–439. doi: 10.1128/iai.36.1.437-439.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  104. Norris S. J. Polypeptides of Treponema pallidum: progress toward understanding their structural, functional, and immunologic roles. Treponema Pallidum Polypeptide Research Group. Microbiol Rev. 1993 Sep;57(3):750–779. doi: 10.1128/mr.57.3.750-779.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  105. PORTINOY J., GARSON W., SMITH C. A. Rapid plasma reagin test for syphilis. Public Health Rep. 1957 Sep;72(9):761–766. [PMC free article] [PubMed] [Google Scholar]
  106. PORTNOY J., BOSSAK H. N., FALCONE V. H., HARRIS A. Rapid reagin test with unheated serum and new improved antigen suspension. Public Health Rep. 1961 Oct;76:933–935. [PMC free article] [PubMed] [Google Scholar]
  107. PORTNOY J., BREWER J. H., HARRIS A. Rapid plasma reagin card test for syphilis and other treponematoses. Public Health Rep. 1962 Aug;77:645–652. [PMC free article] [PubMed] [Google Scholar]
  108. Pavia C. S., Folds J. D., Baseman J. B. Depression of lymphocyte response to concanavalin A in rabbits infected with Treponema pallidum (Nichols strain). Infect Immun. 1976 Jul;14(1):320–322. doi: 10.1128/iai.14.1.320-322.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  109. Pedersen N. S., Orum O., Mouritsen S. Enzyme-linked immunosorbent assay for detection of antibodies to the venereal disease research laboratory (VDRL) antigen in syphilis. J Clin Microbiol. 1987 Sep;25(9):1711–1716. doi: 10.1128/jcm.25.9.1711-1716.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  110. Pedersen N. S., Petersen C. S., Vejtorp M., Axelsen N. H. Serodiagnosis of syphilis by an enzyme-linked immunosorbent assay for IgG antibodies against the Reiter treponeme flagellum. Scand J Immunol. 1982 Apr;15(4):341–348. doi: 10.1111/j.1365-3083.1982.tb00657.x. [DOI] [PubMed] [Google Scholar]
  111. Perine P. L., Nelson J. W., Lewis J. O., Liska S., Hunter E. F., Larsen S. A., Agadzi V. K., Kofi F., Ofori J. A., Tam M. R. New technologies for use in the surveillance and control of yaws. Rev Infect Dis. 1985 May-Jun;7 (Suppl 2):S295–S299. doi: 10.1093/clinids/7-supplement_2.s295. [DOI] [PubMed] [Google Scholar]
  112. Pettit D. E., Larsen S. A., Harbec P. S., Feeley J. C., Parham C. E., Cruce D. D., Hambie E. A., Perryman M. W. Toluidine red unheated serum test, a nontreponemal test for syphilis. J Clin Microbiol. 1983 Nov;18(5):1141–1145. doi: 10.1128/jcm.18.5.1141-1145.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  113. Pettit D. E., Larsen S. A., Pope V., Perryman M. W., Adams M. R. Unheated serum reagin test as a quantitative test for syphilis. J Clin Microbiol. 1982 Feb;15(2):238–242. doi: 10.1128/jcm.15.2.238-242.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  114. Pope V., Hunter E. F., Feeley J. C. Evaluation of the microenzyme-linked immunosorbent assay with Treponema pallidum antigen. J Clin Microbiol. 1982 Apr;15(4):630–634. doi: 10.1128/jcm.15.4.630-634.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  115. Pope V., Larsen S. A., Rice R. J., Goforth S. N., Parham C. E., Fears M. B. Flow cytometric analysis of peripheral blood lymphocyte immunophenotypes in persons infected with Treponema pallidum. Clin Diagn Lab Immunol. 1994 Jan;1(1):121–124. doi: 10.1128/cdli.1.1.121-124.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  116. Radolf J. D., Kaplan R. P. Unusual manifestations of secondary syphilis and abnormal humoral immune response to Treponema pallidum antigens in a homosexual man with asymptomatic human immunodeficiency virus infection. J Am Acad Dermatol. 1988 Feb;18(2 Pt 2):423–428. doi: 10.1016/s0190-9622(88)70062-6. [DOI] [PubMed] [Google Scholar]
  117. Rathlev T. Haemagglutination test utilizing pathogenic Treponema pallidum for the sero-diagnosis of syphilis. Br J Vener Dis. 1967 Sep;43(3):181–185. doi: 10.1136/sti.43.3.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  118. Rawstron S. A., Bromberg K. Comparison of maternal and newborn serologic tests for syphilis. Am J Dis Child. 1991 Dec;145(12):1383–1388. doi: 10.1001/archpedi.1991.02160120051018. [DOI] [PubMed] [Google Scholar]
  119. Reimer C. B., Black C. M., Phillips D. J., Logan L. C., Hunter E. F., Pender B. J., McGrew B. E. The specificity of fetal IgM: antibody or anti-antibody? Ann N Y Acad Sci. 1975 Jun 30;254:77–93. doi: 10.1111/j.1749-6632.1975.tb29159.x. [DOI] [PubMed] [Google Scholar]
  120. Rein M. F., Banks G. W., Logan L. C., Larsen S. A., Feeley J. C., Kellogg D. S., Wiesner P. J. Failure of the Treponema pallidum immobilization test to provide additional diagnostic information about contemporary problem sera. Sex Transm Dis. 1980 Jul-Sep;7(3):101–105. doi: 10.1097/00007435-198007000-00001. [DOI] [PubMed] [Google Scholar]
  121. Rogers M. F., Morens D. M., Stewart J. A., Kaminski R. M., Spira T. J., Feorino P. M., Larsen S. A., Francis D. P., Wilson M., Kaufman L. National case-control study of Kaposi's sarcoma and Pneumocystis carinii pneumonia in homosexual men: Part 2. Laboratory results. Ann Intern Med. 1983 Aug;99(2):151–158. doi: 10.7326/0003-4819-99-2-151. [DOI] [PubMed] [Google Scholar]
  122. Romanowski B., Forsey E., Prasad E., Lukehart S., Tam M., Hook E. W., 3rd Detection of Treponema pallidum by a fluorescent monoclonal antibody test. Sex Transm Dis. 1987 Jul-Sep;14(3):156–159. doi: 10.1097/00007435-198707000-00007. [DOI] [PubMed] [Google Scholar]
  123. Romanowski B., Sutherland R., Fick G. H., Mooney D., Love E. J. Serologic response to treatment of infectious syphilis. Ann Intern Med. 1991 Jun 15;114(12):1005–1009. doi: 10.7326/0003-4819-114-12-1005. [DOI] [PubMed] [Google Scholar]
  124. SPANGLER A. S., JACKSON J. H., FIUMARA N. J., WARTHIN T. A. SYPHILIS WITH A NEGATIVE BLOOD TEST REACTION. JAMA. 1964 Jul 13;189:87–90. doi: 10.1001/jama.1964.03070020015003. [DOI] [PubMed] [Google Scholar]
  125. Salo O. P., Vuorio M., Mustakallio K. K., Aho K. Tests for syphilis in young males. An analysis of a seven-year series from a Central Military Hospital. Mil Med. 1967 Apr;132(4):258–261. [PubMed] [Google Scholar]
  126. Schell R. F., Le Frock J. L., Babu J. P., Chan J. K. Use of CB hamsters in the study of Treponema pertenue. Br J Vener Dis. 1979 Oct;55(5):316–319. doi: 10.1136/sti.55.5.316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  127. Schell R. F., LeFrock J. L., Chan J. K., Bagasra O. LSH hamster model of syphilitic infection and transfer of resistance with immune T cells. Adv Exp Med Biol. 1981;134:291–300. doi: 10.1007/978-1-4757-0495-2_26. [DOI] [PubMed] [Google Scholar]
  128. Schmitz J. L., Gertis K. S., Mauney C., Stamm L. V., Folds J. D. Laboratory diagnosis of congenital syphilis by immunoglobulin M (IgM) and IgA immunoblotting. Clin Diagn Lab Immunol. 1994 Jan;1(1):32–37. doi: 10.1128/cdli.1.1.32-37.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  129. Schouls L. M., Ijsselmuiden O. E., Weel J., van Embden J. D. Overproduction and purification of Treponema pallidum recombinant-DNA-derived proteins TmpA and TmpB and their potential use in serodiagnosis of syphilis. Infect Immun. 1989 Sep;57(9):2612–2623. doi: 10.1128/iai.57.9.2612-2623.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  130. Schouls L. M. Molecular biology of Treponema pallidum. Mol Cell Biol Hum Dis Ser. 1992;1:85–129. doi: 10.1007/978-94-011-2384-6_4. [DOI] [PubMed] [Google Scholar]
  131. Schroeter A. L., Lucas J. B., Price E. V., Falcone V. H. Treatment for early syphilis and reactivity of serologic tests. JAMA. 1972 Jul 31;221(5):471–476. [PubMed] [Google Scholar]
  132. Scotti A. T., Logan L. A specific IgM antibody test in neonatal congenital syphilis. J Pediatr. 1968 Aug;73(2):242–243. doi: 10.1016/s0022-3476(68)80075-7. [DOI] [PubMed] [Google Scholar]
  133. Scotti A., Logan L., Caldwell J. G. Fluorescent antibody test for neonatal congenital syphilis: a progress report. J Pediatr. 1969 Dec;75(6):1129–1134. doi: 10.1016/s0022-3476(69)80368-9. [DOI] [PubMed] [Google Scholar]
  134. Shore R. N., Faricelli J. A. Borderline and reactive FTA-ABS. Results in lupus erythematosus. Arch Dermatol. 1977 Jan;113(1):37–41. [PubMed] [Google Scholar]
  135. Smith J. L., Israel C. W. Spirochetes in the aqueous humor in seronegative ocular syphilis. Persistence after penicillin therapy. Arch Ophthalmol. 1967 Apr;77(4):474–477. doi: 10.1001/archopht.1967.00980020476009. [DOI] [PubMed] [Google Scholar]
  136. Sparling P. F. Diagnosis and treatment of syphilis. N Engl J Med. 1971 Mar 25;284(12):642–653. doi: 10.1056/NEJM197103252841205. [DOI] [PubMed] [Google Scholar]
  137. Stamm L. V., Parrish E. A., Gherardini F. C. Cloning of the recA gene from a free-living leptospire and distribution of RecA-like protein among spirochetes. Appl Environ Microbiol. 1991 Jan;57(1):183–189. doi: 10.1128/aem.57.1.183-189.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  138. Steiner B. M., Wong G. H., Sutrave P., Graves S. Oxygen toxicity in Treponema pallidum: deoxyribonucleic acid single-stranded breakage induced by low doses of hydrogen peroxide. Can J Microbiol. 1984 Dec;30(12):1467–1476. doi: 10.1139/m84-234. [DOI] [PubMed] [Google Scholar]
  139. Stoll B. J., Lee F. K., Larsen S., Hale E., Schwartz D., Rice R. J., Ashby R., Holmes R., Nahmias A. J. Clinical and serologic evaluation of neonates for congenital syphilis: a continuing diagnostic dilemma. J Infect Dis. 1993 May;167(5):1093–1099. doi: 10.1093/infdis/167.5.1093. [DOI] [PubMed] [Google Scholar]
  140. Strugnell R., Cockayne A., Penn C. W. Molecular and antigenic analysis of treponemes. Crit Rev Microbiol. 1990;17(4):231–250. doi: 10.3109/10408419009105727. [DOI] [PubMed] [Google Scholar]
  141. Sánchez P. J., McCracken G. H., Jr, Wendel G. D., Olsen K., Threlkeld N., Norgard M. V. Molecular analysis of the fetal IgM response to Treponema pallidum antigens: implications for improved serodiagnosis of congenital syphilis. J Infect Dis. 1989 Mar;159(3):508–517. doi: 10.1093/infdis/159.3.508. [DOI] [PubMed] [Google Scholar]
  142. Sánchez P. J., Wendel G. D., Jr, Grimprel E., Goldberg M., Hall M., Arencibia-Mireles O., Radolf J. D., Norgard M. V. Evaluation of molecular methodologies and rabbit infectivity testing for the diagnosis of congenital syphilis and neonatal central nervous system invasion by Treponema pallidum. J Infect Dis. 1993 Jan;167(1):148–157. doi: 10.1093/infdis/167.1.148. [DOI] [PubMed] [Google Scholar]
  143. Terpstra W. J., Schoone G. J., ter Schegget J. Detection of leptospiral DNA by nucleic acid hybridisation with 32P- and biotin-labelled probes. J Med Microbiol. 1986 Aug;22(1):23–28. doi: 10.1099/00222615-22-1-23. [DOI] [PubMed] [Google Scholar]
  144. Tomizawa T. Hemagglutination tests for diagnosis of syphilis. A preliminary report. Jpn J Med Sci Biol. 1966 Dec;19(6):305–308. doi: 10.7883/yoken1952.19.305. [DOI] [PubMed] [Google Scholar]
  145. Tomizawa T., Kasamatsu S., Yamaya S. I. Usefulness of the hemagglutination test using Treponema pallidum antigen (TPHA) for the serodiagnosis of syphilis. Jpn J Med Sci Biol. 1969 Dec;22(6):341–350. doi: 10.7883/yoken1952.22.341. [DOI] [PubMed] [Google Scholar]
  146. Tuffanelli D. L. Ageing and false positive reactions for syphilis. Br J Vener Dis. 1966 Mar;42(1):40–41. doi: 10.1136/sti.42.1.40. [DOI] [PMC free article] [PubMed] [Google Scholar]
  147. Van Dyck E., Van de Velden L., Ndoye I., Piot P., Meheus A. Evaluation of the rapid plasma reagin "teardrop" card test for screening of syphilis in field conditions. Sex Transm Dis. 1993 Jul-Aug;20(4):194–197. doi: 10.1097/00007435-199307000-00003. [DOI] [PubMed] [Google Scholar]
  148. Veldkamp J., Visser A. M. Application of the enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of syphilis. Br J Vener Dis. 1975 Aug;51(4):227–231. doi: 10.1136/sti.51.4.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  149. Viscidi R. P., Yolken R. G. Molecular diagnosis of infectious diseases by nucleic acid hybridization. Mol Cell Probes. 1987 Mar;1(1):3–14. doi: 10.1016/0890-8508(87)90003-x. [DOI] [PubMed] [Google Scholar]
  150. Wentworth B. B., Thompson M. A., Peter C. R., Bawdon R. E., Wilson D. L. Comparison of a hemagglutination treponemal test for syphilis (HATTS) with other serologic methods for the diagnosis of syphilis. Sex Transm Dis. 1978 Jul-Sep;5(3):103–114. doi: 10.1097/00007435-197807000-00005. [DOI] [PubMed] [Google Scholar]
  151. White T. J., Fuller S. A. Visuwell Reagin, a non-treponemal enzyme-linked immunosorbent assay for the serodiagnosis of syphilis. J Clin Microbiol. 1989 Oct;27(10):2300–2304. doi: 10.1128/jcm.27.10.2300-2304.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  152. Wicher K., Noordhoek G. T., Abbruscato F., Wicher V. Detection of Treponema pallidum in early syphilis by DNA amplification. J Clin Microbiol. 1992 Feb;30(2):497–500. doi: 10.1128/jcm.30.2.497-500.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  153. Wilkinson A. E., Cowell L. P. Immunofluorescent staining for the detection of Treponema pallidum in early syphilitic lesions. Br J Vener Dis. 1971 Aug;47(4):252–254. doi: 10.1136/sti.47.4.252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  154. Wilkinson A. E. Fluorescent treponemal antibody tests on cerebrospinal fluid. Br J Vener Dis. 1973 Aug;49(4):346–349. doi: 10.1136/sti.49.4.346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  155. Wilkinson H. W., Sampson J. S., Plikaytis B. B. Evaluation of a commercial gene probe for identification of Legionella cultures. J Clin Microbiol. 1986 Feb;23(2):217–220. doi: 10.1128/jcm.23.2.217-220.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  156. YOBS A. R., BROWN L., HUNTER E. F. FLUORESCENT ANTIBODY TECHNIQUE IN EARLY SYPHILIS; AS APPLIED TO THE DEMONSTRATION OF T PALLIDUM IN LESIONS IN THE RABBIT AND IN THE HUMAN. Arch Pathol. 1964 Feb;77:220–225. [PubMed] [Google Scholar]
  157. Young H., Moyes A., McMillan A., Patterson J. Enzyme immunoassay for anti-treponemal IgG: screening or confirmatory test? J Clin Pathol. 1992 Jan;45(1):37–41. doi: 10.1136/jcp.45.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  158. Zenker P. N., Berman S. M. Congenital syphilis: reporting and reality. Am J Public Health. 1990 Mar;80(3):271–272. doi: 10.2105/ajph.80.3.271. [DOI] [PMC free article] [PubMed] [Google Scholar]

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