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. 1997 Apr;10(2):242–256. doi: 10.1128/cmr.10.2.242

Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory.

M Ieven 1, H Goossens 1
PMCID: PMC172918  PMID: 9105753

Abstract

Clinical laboratories are increasingly receiving requests to perform nucleic acid amplification tests for the detection of a wide variety of infectious agents. In this paper, the efficiency of nucleic acid amplification techniques for the diagnosis of respiratory tract infections is reviewed. In general, these techniques should be applied only for the detection of microorganisms for which available diagnostic techniques are markedly insensitive or nonexistent or when turnaround times for existing tests (e.g., viral culture) are much longer than those expected with amplification. This is the case for rhinoviruses, coronaviruses, and hantaviruses causing a pulmonary syndrome, Bordetella pertussis, Chlamydia pneumoniae, Mycoplasma pneumoniae, and Coxiella burnetii. For Legionella spp. and fungi, contamination originating from the environment is a limiting factor in interpretation of results, as is the difficulty in differentiating colonization and infection. Detection of these agents in urine or blood by amplification techniques remains to be evaluated. In the clinical setting, there is no need for molecular diagnostic tests for the diagnosis of Pneumocystis carinii. At present, amplification methods for Mycobacterium tuberculosis cannot replace the classical diagnostic techniques, due to their lack of sensitivity and the absence of specific internal controls for the detection of inhibitors of the reaction. Also, the results of interlaboratory comparisons are unsatisfactory. Furthermore, isolates are needed for susceptibility studies. Additional work remains to be done on sample preparation methods, comparison between different amplification methods, and analysis of results. The techniques can be useful for the rapid identification of M. tuberculosis in particular circumstances, as well as the rapid detection of most rifampin-resistant isolates. The introduction of diagnostic amplification techniques into a clinical laboratory implies a level of proficiency for excluding false-positive and false-negative results.

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Selected References

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  1. Abe C., Hirano K., Wada M., Kazumi Y., Takahashi M., Fukasawa Y., Yoshimura T., Miyagi C., Goto S. Detection of Mycobacterium tuberculosis in clinical specimens by polymerase chain reaction and Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test. J Clin Microbiol. 1993 Dec;31(12):3270–3274. doi: 10.1128/jcm.31.12.3270-3274.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alexander E. R., Harrison H. R. Role of Chlamydia trachomatis in perinatal infection. Rev Infect Dis. 1983 Jul-Aug;5(4):713–719. doi: 10.1093/clinids/5.4.713. [DOI] [PubMed] [Google Scholar]
  3. Amicosante M., Richeldi L., Trenti G., Paone G., Campa M., Bisetti A., Saltini C. Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin. J Clin Microbiol. 1995 Mar;33(3):629–630. doi: 10.1128/jcm.33.3.629-630.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. An Q., Buxton D., Hendricks A., Robinson L., Shah J., Lu L., Vera-Garcia M., King W., Olive D. M. Comparison of amplified Q beta replicase and PCR assays for detection of Mycobacterium tuberculosis. J Clin Microbiol. 1995 Apr;33(4):860–867. doi: 10.1128/jcm.33.4.860-867.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Andersen A. B., Thybo S., Godfrey-Faussett P., Stoker N. G. Polymerase chain reaction for detection of Mycobacterium tuberculosis in sputum. Eur J Clin Microbiol Infect Dis. 1993 Dec;12(12):922–927. doi: 10.1007/BF01992166. [DOI] [PubMed] [Google Scholar]
  6. Arruda E., Hayden F. G. Detection of human rhinovirus RNA in nasal washings by PCR. Mol Cell Probes. 1993 Oct;7(5):373–379. doi: 10.1006/mcpr.1993.1055. [DOI] [PubMed] [Google Scholar]
  7. Beavis K. G., Lichty M. B., Jungkind D. L., Giger O. Evaluation of Amplicor PCR for direct detection of Mycobacterium tuberculosis from sputum specimens. J Clin Microbiol. 1995 Oct;33(10):2582–2586. doi: 10.1128/jcm.33.10.2582-2586.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Beige J., Lokies J., Schaberg T., Finckh U., Fischer M., Mauch H., Lode H., Köhler B., Rolfs A. Clinical evaluation of a Mycobacterium tuberculosis PCR assay. J Clin Microbiol. 1995 Jan;33(1):90–95. doi: 10.1128/jcm.33.1.90-95.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Black C. M., Fields P. I., Messmer T. O., Berdal B. P. Detection of Chlamydia pneumoniae in clinical specimens by polymerase chain reaction using nested primers. Eur J Clin Microbiol Infect Dis. 1994 Sep;13(9):752–756. doi: 10.1007/BF02276060. [DOI] [PubMed] [Google Scholar]
  10. Black C. M., Tharpe J. A., Russell H. Distinguishing Chlamydia species by restriction analysis of the major outer membrane protein gene. Mol Cell Probes. 1992 Oct;6(5):395–400. doi: 10.1016/0890-8508(92)90033-t. [DOI] [PubMed] [Google Scholar]
  11. Bodmer T., Gurtner A., Schopfer K., Matter L. Screening of respiratory tract specimens for the presence of Mycobacterium tuberculosis by using the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test. J Clin Microbiol. 1994 Jun;32(6):1483–1487. doi: 10.1128/jcm.32.6.1483-1487.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Bodmer T., Möckl E., Mühlemann K., Matter L. Improved performance of Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test when 500 instead of 50 microliters of decontaminated sediment is used. J Clin Microbiol. 1996 Jan;34(1):222–223. doi: 10.1128/jcm.34.1.222-223.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Boom R., Sol C. J., Salimans M. M., Jansen C. L., Wertheim-van Dillen P. M., van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol. 1990 Mar;28(3):495–503. doi: 10.1128/jcm.28.3.495-503.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Bretagne S., Costa J. M., Marmorat-Khuong A., Poron F., Cordonnier C., Vidaud M., Fleury-Feith J. Detection of Aspergillus species DNA in bronchoalveolar lavage samples by competitive PCR. J Clin Microbiol. 1995 May;33(5):1164–1168. doi: 10.1128/jcm.33.5.1164-1168.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Bruce C. B., al-Nakib W., Almond J. W., Tyrrell D. A. Use of synthetic oligonucleotide probes to detect rhinovirus RNA. Arch Virol. 1989;105(3-4):179–187. doi: 10.1007/BF01311355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Buck G. E., O'Hara L. C., Summersgill J. T. Rapid, simple method for treating clinical specimens containing Mycobacterium tuberculosis to remove DNA for polymerase chain reaction. J Clin Microbiol. 1992 May;30(5):1331–1334. doi: 10.1128/jcm.30.5.1331-1334.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Burgener-Kairuz P., Zuber J. P., Jaunin P., Buchman T. G., Bille J., Rossier M. Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species-specific nested PCR amplification of a cytochrome P-450 lanosterol-alpha-demethylase (L1A1) gene fragment. J Clin Microbiol. 1994 Aug;32(8):1902–1907. doi: 10.1128/jcm.32.8.1902-1907.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Bäckman A., Johansson B., Olcén P. Nested PCR optimized for detection of Bordetella pertussis in clinical nasopharyngeal samples. J Clin Microbiol. 1994 Oct;32(10):2544–2548. doi: 10.1128/jcm.32.10.2544-2548.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Campbell L. A., Perez Melgosa M., Hamilton D. J., Kuo C. C., Grayston J. T. Detection of Chlamydia pneumoniae by polymerase chain reaction. J Clin Microbiol. 1992 Feb;30(2):434–439. doi: 10.1128/jcm.30.2.434-439.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Carpentier E., Drouillard B., Dailloux M., Moinard D., Vallee E., Dutilh B., Maugein J., Bergogne-Berezin E., Carbonnelle B. Diagnosis of tuberculosis by Amplicor Mycobacterium tuberculosis test: a multicenter study. J Clin Microbiol. 1995 Dec;33(12):3106–3110. doi: 10.1128/jcm.33.12.3106-3110.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Cartwright C. P., Nelson N. A., Gill V. J. Development and evaluation of a rapid and simple procedure for detection of Pneumocystis carinii by PCR. J Clin Microbiol. 1994 Jul;32(7):1634–1638. doi: 10.1128/jcm.32.7.1634-1638.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Chryssanthou E., Andersson B., Petrini B., Löfdahl S., Tollemar J. Detection of Candida albicans DNA in serum by polymerase chain reaction. Scand J Infect Dis. 1994;26(4):479–485. doi: 10.3109/00365549409008623. [DOI] [PubMed] [Google Scholar]
  23. Claas E. C., van Milaan A. J., Sprenger M. J., Ruiten-Stuiver M., Arron G. I., Rothbarth P. H., Masurel N. Prospective application of reverse transcriptase polymerase chain reaction for diagnosing influenza infections in respiratory samples from a children's hospital. J Clin Microbiol. 1993 Aug;31(8):2218–2221. doi: 10.1128/jcm.31.8.2218-2221.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Clarridge J. E., 3rd, Shawar R. M., Shinnick T. M., Plikaytis B. B. Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine mycobacteriology laboratory. J Clin Microbiol. 1993 Aug;31(8):2049–2056. doi: 10.1128/jcm.31.8.2049-2056.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Compton J. Nucleic acid sequence-based amplification. Nature. 1991 Mar 7;350(6313):91–92. doi: 10.1038/350091a0. [DOI] [PubMed] [Google Scholar]
  26. Crampin A. C., Matthews R. C. Application of the polymerase chain reaction to the diagnosis of candidosis by amplification of an HSP 90 gene fragment. J Med Microbiol. 1993 Sep;39(3):233–238. doi: 10.1099/00222615-39-3-233. [DOI] [PubMed] [Google Scholar]
  27. D'Amato R. F., Wallman A. A., Hochstein L. H., Colaninno P. M., Scardamaglia M., Ardila E., Ghouri M., Kim K., Patel R. C., Miller A. Rapid diagnosis of pulmonary tuberculosis by using Roche AMPLICOR Mycobacterium tuberculosis PCR test. J Clin Microbiol. 1995 Jul;33(7):1832–1834. doi: 10.1128/jcm.33.7.1832-1834.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Daniel T. M. The rapid diagnosis of tuberculosis: a selective review. J Lab Clin Med. 1990 Sep;116(3):277–282. [PubMed] [Google Scholar]
  29. Das S., Chan S. L., Allen B. W., Mitchison D. A., Lowrie D. B. Application of DNA fingerprinting with IS986 to sequential mycobacterial isolates obtained from pulmonary tuberculosis patients in Hong Kong before, during and after short-course chemotherapy. Tuber Lung Dis. 1993 Feb;74(1):47–51. doi: 10.1016/0962-8479(93)90068-9. [DOI] [PubMed] [Google Scholar]
  30. De Beenhouwer H., Lhiang Z., Jannes G., Mijs W., Machtelinckx L., Rossau R., Traore H., Portaels F. Rapid detection of rifampicin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assay. Tuber Lung Dis. 1995 Oct;76(5):425–430. doi: 10.1016/0962-8479(95)90009-8. [DOI] [PubMed] [Google Scholar]
  31. Douglas E., Coote J. G., Parton R., McPheat W. Identification of Bordetella pertussis in nasopharyngeal swabs by PCR amplification of a region of the adenylate cyclase gene. J Med Microbiol. 1993 Feb;38(2):140–144. doi: 10.1099/00222615-38-2-140. [DOI] [PubMed] [Google Scholar]
  32. Dumornay W., Roblin P. M., Gelling M., Hammerschlag M. R., Worku M. Comparison of a chemiluminometric immunoassay with culture for diagnosis of chlamydial infections in infants. J Clin Microbiol. 1992 Jul;30(7):1867–1869. doi: 10.1128/jcm.30.7.1867-1869.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Eisen D., Ross B. C., Fairbairn J., Warren R. J., Baird R. W., Dwyer B. Comparison of Pneumocystis carinii detection by toluidine blue O staining, direct immunofluorescence and DNA amplification in sputum specimens from HIV positive patients. Pathology. 1994 Apr;26(2):198–200. doi: 10.1080/00313029400169471. [DOI] [PubMed] [Google Scholar]
  34. Eisenach K. D., Cave M. D., Bates J. H., Crawford J. T. Polymerase chain reaction amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis. J Infect Dis. 1990 May;161(5):977–981. doi: 10.1093/infdis/161.5.977. [DOI] [PubMed] [Google Scholar]
  35. Eisenach K. D., Sifford M. D., Cave M. D., Bates J. H., Crawford J. T. Detection of Mycobacterium tuberculosis in sputum samples using a polymerase chain reaction. Am Rev Respir Dis. 1991 Nov;144(5):1160–1163. doi: 10.1164/ajrccm/144.5.1160. [DOI] [PubMed] [Google Scholar]
  36. Ellner P. D., Kiehn T. E., Cammarata R., Hosmer M. Rapid detection and identification of pathogenic mycobacteria by combining radiometric and nucleic acid probe methods. J Clin Microbiol. 1988 Jul;26(7):1349–1352. doi: 10.1128/jcm.26.7.1349-1352.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Ewanowich C. A., Chui L. W., Paranchych M. G., Peppler M. S., Marusyk R. G., Albritton W. L. Major outbreak of pertussis in northern Alberta, Canada: analysis of discrepant direct fluorescent-antibody and culture results by using polymerase chain reaction methodology. J Clin Microbiol. 1993 Jul;31(7):1715–1725. doi: 10.1128/jcm.31.7.1715-1725.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Felmlee T. A., Liu Q., Whelen A. C., Williams D., Sommer S. S., Persing D. H. Genotypic detection of Mycobacterium tuberculosis rifampin resistance: comparison of single-strand conformation polymorphism and dideoxy fingerprinting. J Clin Microbiol. 1995 Jun;33(6):1617–1623. doi: 10.1128/jcm.33.6.1617-1623.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Fliermans C. B., Cherry W. B., Orrison L. H., Smith S. J., Tison D. L., Pope D. H. Ecological distribution of Legionella pneumophila. Appl Environ Microbiol. 1981 Jan;41(1):9–16. doi: 10.1128/aem.41.1.9-16.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Folgueira L., Delgado R., Palenque E., Aguado J. M., Noriega A. R. Rapid diagnosis of Mycobacterium tuberculosis bacteremia by PCR. J Clin Microbiol. 1996 Mar;34(3):512–515. doi: 10.1128/jcm.34.3.512-515.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Forbes B. A., Hicks K. E. Ability of PCR assay to identify Mycobacterium tuberculosis in BACTEC 12B vials. J Clin Microbiol. 1994 Jul;32(7):1725–1728. doi: 10.1128/jcm.32.7.1725-1728.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Forbes B. A., Hicks K. E. Direct detection of Mycobacterium tuberculosis in respiratory specimens in a clinical laboratory by polymerase chain reaction. J Clin Microbiol. 1993 Jul;31(7):1688–1694. doi: 10.1128/jcm.31.7.1688-1694.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Gama R. E., Horsnell P. R., Hughes P. J., North C., Bruce C. B., al-Nakib W., Stanway G. Amplification of rhinovirus specific nucleic acids from clinical samples using the polymerase chain reaction. J Med Virol. 1989 Jun;28(2):73–77. doi: 10.1002/jmv.1890280204. [DOI] [PubMed] [Google Scholar]
  44. Gaydos C. A., Eiden J. J., Oldach D., Mundy L. M., Auwaerter P., Warner M. L., Vance E., Burton A. A., Quinn T. C. Diagnosis of Chlamydia pneumoniae infection in patients with community-acquired pneumonia by polymerase chain reaction enzyme immunoassay. Clin Infect Dis. 1994 Jul;19(1):157–160. doi: 10.1093/clinids/19.1.157. [DOI] [PubMed] [Google Scholar]
  45. Gaydos C. A., Fowler C. L., Gill V. J., Eiden J. J., Quinn T. C. Detection of Chlamydia pneumoniae by polymerase chain reaction-enzyme immunoassay in an immunocompromised population. Clin Infect Dis. 1993 Oct;17(4):718–723. doi: 10.1093/clinids/17.4.718. [DOI] [PubMed] [Google Scholar]
  46. Gaydos C. A., Quinn T. C., Eiden J. J. Identification of Chlamydia pneumoniae by DNA amplification of the 16S rRNA gene. J Clin Microbiol. 1992 Apr;30(4):796–800. doi: 10.1128/jcm.30.4.796-800.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Gaydos C. A., Roblin P. M., Hammerschlag M. R., Hyman C. L., Eiden J. J., Schachter J., Quinn T. C. Diagnostic utility of PCR-enzyme immunoassay, culture, and serology for detection of Chlamydia pneumoniae in symptomatic and asymptomatic patients. J Clin Microbiol. 1994 Apr;32(4):903–905. doi: 10.1128/jcm.32.4.903-905.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. Gilbert L. L., Dakhama A., Bone B. M., Thomas E. E., Hegele R. G. Diagnosis of viral respiratory tract infections in children by using a reverse transcription-PCR panel. J Clin Microbiol. 1996 Jan;34(1):140–143. doi: 10.1128/jcm.34.1.140-143.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Gingeras T. R., Prodanovich P., Latimer T., Guatelli J. C., Richman D. D., Barringer K. J. Use of self-sustained sequence replication amplification reaction to analyze and detect mutations in zidovudine-resistant human immunodeficiency virus. J Infect Dis. 1991 Dec;164(6):1066–1074. doi: 10.1093/infdis/164.6.1066. [DOI] [PubMed] [Google Scholar]
  50. Gnarpe J., Eriksson K. Sample preparation for Chlamydia pneumoniae PCR. APMIS. 1995 Apr;103(4):307–308. [PubMed] [Google Scholar]
  51. Goessens W. H., Kluytmans J. A., den Toom N., van Rijsoort-Vos T. H., Niesters B. G., Stolz E., Verbrugh H. A., Quint W. G. Influence of volume of sample processed on detection of Chlamydia trachomatis in urogenital samples by PCR. J Clin Microbiol. 1995 Jan;33(1):251–253. doi: 10.1128/jcm.33.1.251-253.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  52. Grayston J. T., Aldous M. B., Easton A., Wang S. P., Kuo C. C., Campbell L. A., Altman J. Evidence that Chlamydia pneumoniae causes pneumonia and bronchitis. J Infect Dis. 1993 Nov;168(5):1231–1235. doi: 10.1093/infdis/168.5.1231. [DOI] [PubMed] [Google Scholar]
  53. Grayston J. T., Wang S. P., Kuo C. C., Campbell L. A. Current knowledge on Chlamydia pneumoniae, strain TWAR, an important cause of pneumonia and other acute respiratory diseases. Eur J Clin Microbiol Infect Dis. 1989 Mar;8(3):191–202. doi: 10.1007/BF01965260. [DOI] [PubMed] [Google Scholar]
  54. Grimprel E., Bégué P., Anjak I., Betsou F., Guiso N. Comparison of polymerase chain reaction, culture, and western immunoblot serology for diagnosis of Bordetella pertussis infection. J Clin Microbiol. 1993 Oct;31(10):2745–2750. doi: 10.1128/jcm.31.10.2745-2750.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  55. Halonen P., Rocha E., Hierholzer J., Holloway B., Hyypiä T., Hurskainen P., Pallansch M. Detection of enteroviruses and rhinoviruses in clinical specimens by PCR and liquid-phase hybridization. J Clin Microbiol. 1995 Mar;33(3):648–653. doi: 10.1128/jcm.33.3.648-653.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  56. Halperin S. A., Bortolussi R., Wort A. J. Evaluation of culture, immunofluorescence, and serology for the diagnosis of pertussis. J Clin Microbiol. 1989 Apr;27(4):752–757. doi: 10.1128/jcm.27.4.752-757.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  57. Hata D., Kuze F., Mochizuki Y., Ohkubo H., Kanazashi S., Maeda S., Miwa N., Mikawa H. Evaluation of DNA probe test for rapid diagnosis of Mycoplasma pneumoniae infections. J Pediatr. 1990 Feb;116(2):273–276. doi: 10.1016/s0022-3476(05)82889-6. [DOI] [PubMed] [Google Scholar]
  58. He Q., Marjamäki M., Soini H., Mertsola J., Viljanen M. K. Primers are decisive for sensitivity of PCR. Biotechniques. 1994 Jul;17(1):82, 84, 86-7. [PubMed] [Google Scholar]
  59. He Q., Mertsola J., Soini H., Skurnik M., Ruuskanen O., Viljanen M. K. Comparison of polymerase chain reaction with culture and enzyme immunoassay for diagnosis of pertussis. J Clin Microbiol. 1993 Mar;31(3):642–645. doi: 10.1128/jcm.31.3.642-645.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  60. He Q., Mertsola J., Soini H., Viljanen M. K. Sensitive and specific polymerase chain reaction assays for detection of Bordetella pertussis in nasopharyngeal specimens. J Pediatr. 1994 Mar;124(3):421–426. doi: 10.1016/s0022-3476(94)70365-5. [DOI] [PubMed] [Google Scholar]
  61. Hellyer T. J., Fletcher T. W., Bates J. H., Stead W. W., Templeton G. L., Cave M. D., Eisenach K. D. Strand displacement amplification and the polymerase chain reaction for monitoring response to treatment in patients with pulmonary tuberculosis. J Infect Dis. 1996 Apr;173(4):934–941. doi: 10.1093/infdis/173.4.934. [DOI] [PubMed] [Google Scholar]
  62. Hermans P. W., Schuitema A. R., Van Soolingen D., Verstynen C. P., Bik E. M., Thole J. E., Kolk A. H., van Embden J. D. Specific detection of Mycobacterium tuberculosis complex strains by polymerase chain reaction. J Clin Microbiol. 1990 Jun;28(6):1204–1213. doi: 10.1128/jcm.28.6.1204-1213.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  63. Hermans P. W., van Soolingen D., Dale J. W., Schuitema A. R., McAdam R. A., Catty D., van Embden J. D. Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis. J Clin Microbiol. 1990 Sep;28(9):2051–2058. doi: 10.1128/jcm.28.9.2051-2058.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  64. Herrera E. A., Segovia M. Evaluation of mtp40 genomic fragment amplification for specific detection of Mycobacterium tuberculosis in clinical specimens. J Clin Microbiol. 1996 May;34(5):1108–1113. doi: 10.1128/jcm.34.5.1108-1113.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  65. Holland S. M., Gaydos C. A., Quinn T. C. Detection and differentiation of Chlamydia trachomatis, Chlamydia psittaci, and Chlamydia pneumoniae by DNA amplification. J Infect Dis. 1990 Oct;162(4):984–987. doi: 10.1093/infdis/162.4.984. [DOI] [PubMed] [Google Scholar]
  66. Holmes A. R., Cannon R. D., Shepherd M. G., Jenkinson H. F. Detection of Candida albicans and other yeasts in blood by PCR. J Clin Microbiol. 1994 Jan;32(1):228–231. doi: 10.1128/jcm.32.1.228-231.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  67. Hopfer R. L., Walden P., Setterquist S., Highsmith W. E. Detection and differentiation of fungi in clinical specimens using polymerase chain reaction (PCR) amplification and restriction enzyme analysis. J Med Vet Mycol. 1993;31(1):65–75. doi: 10.1080/02681219380000071. [DOI] [PubMed] [Google Scholar]
  68. Hopfer R. L., Walden P., Setterquist S., Highsmith W. E. Detection and differentiation of fungi in clinical specimens using polymerase chain reaction (PCR) amplification and restriction enzyme analysis. J Med Vet Mycol. 1993;31(1):65–75. doi: 10.1080/02681219380000071. [DOI] [PubMed] [Google Scholar]
  69. Hunt J. M., Roberts G. D., Stockman L., Felmlee T. A., Persing D. H. Detection of a genetic locus encoding resistance to rifampin in mycobacterial cultures and in clinical specimens. Diagn Microbiol Infect Dis. 1994 Apr;18(4):219–227. doi: 10.1016/0732-8893(94)90024-8. [DOI] [PubMed] [Google Scholar]
  70. Hyman C. L., Roblin P. M., Gaydos C. A., Quinn T. C., Schachter J., Hammerschlag M. R. Prevalence of asymptomatic nasopharyngeal carriage of Chlamydia pneumoniae in subjectively healthy adults: assessment by polymerase chain reaction-enzyme immunoassay and culture. Clin Infect Dis. 1995 May;20(5):1174–1178. doi: 10.1093/clinids/20.5.1174. [DOI] [PubMed] [Google Scholar]
  71. Ichiyama S., Iinuma Y., Tawada Y., Yamori S., Hasegawa Y., Shimokata K., Nakashima N. Evaluation of Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test and Roche PCR-microwell plate hybridization method (AMPLICOR MYCOBACTERIUM) for direct detection of mycobacteria. J Clin Microbiol. 1996 Jan;34(1):130–133. doi: 10.1128/jcm.34.1.130-133.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  72. Ieven M., Ursi D., Van Bever H., Quint W., Niesters H. G., Goossens H. Detection of Mycoplasma pneumoniae by two polymerase chain reactions and role of M. pneumoniae in acute respiratory tract infections in pediatric patients. J Infect Dis. 1996 Jun;173(6):1445–1452. doi: 10.1093/infdis/173.6.1445. [DOI] [PubMed] [Google Scholar]
  73. Inamine J. M., Denny T. P., Loechel S., Schaper U., Huang C. H., Bott K. F., Hu P. C. Nucleotide sequence of the P1 attachment-protein gene of Mycoplasma pneumoniae. Gene. 1988 Apr 29;64(2):217–229. doi: 10.1016/0378-1119(88)90337-x. [DOI] [PubMed] [Google Scholar]
  74. Iovannisci D. M., Winn-Deen E. S. Ligation amplification and fluorescence detection of Mycobacterium tuberculosis DNA. Mol Cell Probes. 1993 Feb;7(1):35–43. doi: 10.1006/mcpr.1993.1005. [DOI] [PubMed] [Google Scholar]
  75. Ireland D. C., Kent J., Nicholson K. G. Improved detection of rhinoviruses in nasal and throat swabs by seminested RT-PCR. J Med Virol. 1993 Jun;40(2):96–101. doi: 10.1002/jmv.1890400204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  76. Jaulhac B., Nowicki M., Bornstein N., Meunier O., Prevost G., Piemont Y., Fleurette J., Monteil H. Detection of Legionella spp. in bronchoalveolar lavage fluids by DNA amplification. J Clin Microbiol. 1992 Apr;30(4):920–924. doi: 10.1128/jcm.30.4.920-924.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  77. Johnston S. L., Sanderson G., Pattemore P. K., Smith S., Bardin P. G., Bruce C. B., Lambden P. R., Tyrrell D. A., Holgate S. T. Use of polymerase chain reaction for diagnosis of picornavirus infection in subjects with and without respiratory symptoms. J Clin Microbiol. 1993 Jan;31(1):111–117. doi: 10.1128/jcm.31.1.111-117.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  78. Jonas V., Alden M. J., Curry J. I., Kamisango K., Knott C. A., Lankford R., Wolfe J. M., Moore D. F. Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by amplification of rRNA. J Clin Microbiol. 1993 Sep;31(9):2410–2416. doi: 10.1128/jcm.31.9.2410-2416.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  79. Jordan JA, Durso MB. Rapid Speciation of the Five Most Medically Relevant Candida Species Using PCR Amplification and a Microtiter Plate-Based Detection System. Mol Diagn. 1996 Jun;1(1):51–58. doi: 10.1054/MODI00100051. [DOI] [PubMed] [Google Scholar]
  80. KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. Harvey Lect. 1957;53:83–112. [PubMed] [Google Scholar]
  81. Kai M., Kamiya S., Yabe H., Takakura I., Shiozawa K., Ozawa A. Rapid detection of Mycoplasma pneumoniae in clinical samples by the polymerase chain reaction. J Med Microbiol. 1993 Mar;38(3):166–170. doi: 10.1099/00222615-38-3-166. [DOI] [PubMed] [Google Scholar]
  82. Kan V. L. Polymerase chain reaction for the diagnosis of candidemia. J Infect Dis. 1993 Sep;168(3):779–783. doi: 10.1093/infdis/168.3.779. [DOI] [PubMed] [Google Scholar]
  83. Kaneko S., Murakami S., Unoura M., Kobayashi K. Quantitation of hepatitis C virus RNA by competitive polymerase chain reaction. J Med Virol. 1992 Aug;37(4):278–282. doi: 10.1002/jmv.1890370408. [DOI] [PubMed] [Google Scholar]
  84. Kapur V., Li L. L., Iordanescu S., Hamrick M. R., Wanger A., Kreiswirth B. N., Musser J. M. Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase beta subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas. J Clin Microbiol. 1994 Apr;32(4):1095–1098. doi: 10.1128/jcm.32.4.1095-1098.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  85. Kessler H. H., Reinthaler F. F., Pschaid A., Pierer K., Kleinhappl B., Eber E., Marth E. Rapid detection of Legionella species in bronchoalveolar lavage fluids with the EnviroAmp Legionella PCR amplification and detection kit. J Clin Microbiol. 1993 Dec;31(12):3325–3328. doi: 10.1128/jcm.31.12.3325-3328.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  86. Kirschner P., Springer B., Vogel U., Meier A., Wrede A., Kiekenbeck M., Bange F. C., Böttger E. C. Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J Clin Microbiol. 1993 Nov;31(11):2882–2889. doi: 10.1128/jcm.31.11.2882-2889.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  87. Kitada K., Oka S., Kohjin T., Kimura S., Nakamura Y., Shimada K. Pneumocystis carinii pneumonia monitored by P. carinii shedding in sputum by the polymerase chain reaction. Intern Med. 1993 May;32(5):370–373. doi: 10.2169/internalmedicine.32.370. [DOI] [PubMed] [Google Scholar]
  88. Kleemola M., Heiskanen-Kosma T., Nohynek H., Jokinen S., Korppi M., Eskola J. Diagnostic efficacy of a Mycoplasma pneumoniae hybridization test in nasopharyngeal aspirates of children. Pediatr Infect Dis J. 1993 Apr;12(4):344–345. doi: 10.1097/00006454-199304000-00015. [DOI] [PubMed] [Google Scholar]
  89. Kleemola S. R., Karjalainen J. E., Räty R. K. Rapid diagnosis of Mycoplasma pneumoniae infection: clinical evaluation of a commercial probe test. J Infect Dis. 1990 Jul;162(1):70–75. doi: 10.1093/infdis/162.1.70. [DOI] [PubMed] [Google Scholar]
  90. Kocagöz T., Yilmaz E., Ozkara S., Kocagöz S., Hayran M., Sachedeva M., Chambers H. F. Detection of Mycobacterium tuberculosis in sputum samples by polymerase chain reaction using a simplified procedure. J Clin Microbiol. 1993 Jun;31(6):1435–1438. doi: 10.1128/jcm.31.6.1435-1438.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  91. Kolk A. H., Noordhoek G. T., de Leeuw O., Kuijper S., van Embden J. D. Mycobacterium smegmatis strain for detection of Mycobacterium tuberculosis by PCR used as internal control for inhibition of amplification and for quantification of bacteria. J Clin Microbiol. 1994 May;32(5):1354–1356. doi: 10.1128/jcm.32.5.1354-1356.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  92. Ksiazek T. G., Peters C. J., Rollin P. E., Zaki S., Nichol S., Spiropoulou C., Morzunov S., Feldmann H., Sanchez A., Khan A. S. Identification of a new North American hantavirus that causes acute pulmonary insufficiency. Am J Trop Med Hyg. 1995 Feb;52(2):117–123. doi: 10.4269/ajtmh.1995.52.117. [DOI] [PubMed] [Google Scholar]
  93. Lamblin G., Rahmoune H., Wieruszeski J. M., Lhermitte M., Strecker G., Roussel P. Structure of two sulphated oligosaccharides from respiratory mucins of a patient suffering from cystic fibrosis. A fast-atom-bombardment m.s. and 1H-n.m.r. spectroscopic study. Biochem J. 1991 Apr 1;275(Pt 1):199–206. doi: 10.1042/bj2750199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  94. Landegren U., Kaiser R., Sanders J., Hood L. A ligase-mediated gene detection technique. Science. 1988 Aug 26;241(4869):1077–1080. doi: 10.1126/science.3413476. [DOI] [PubMed] [Google Scholar]
  95. Leigh T. R., Gazzard B. G., Rowbottom A., Collins J. V. Quantitative and qualitative comparison of DNA amplification by PCR with immunofluorescence staining for diagnosis of Pneumocystis carinii pneumonia. J Clin Pathol. 1993 Feb;46(2):140–144. doi: 10.1136/jcp.46.2.140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  96. Leng Z., Kenny G. E., Roberts M. C. Evaluation of the detection limits of PCR for identification of Mycoplasma pneumoniae in clinical samples. Mol Cell Probes. 1994 Apr;8(2):125–130. doi: 10.1006/mcpr.1994.1017. [DOI] [PubMed] [Google Scholar]
  97. Lipschik G. Y., Gill V. J., Lundgren J. D., Andrawis V. A., Nelson N. A., Nielsen J. O., Ognibene F. P., Kovacs J. A. Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood. Lancet. 1992 Jul 25;340(8813):203–206. doi: 10.1016/0140-6736(92)90469-j. [DOI] [PubMed] [Google Scholar]
  98. Liu Z., Stout J. E., Tedesco L., Boldin M., Hwang C., Diven W. F., Yu V. L. Controlled evaluation of copper-silver ionization in eradicating Legionella pneumophila from a hospital water distribution system. J Infect Dis. 1994 Apr;169(4):919–922. doi: 10.1093/infdis/169.4.919. [DOI] [PubMed] [Google Scholar]
  99. Lomeli H., Tyagi S., Pritchard C. G., Lizardi P. M., Kramer F. R. Quantitative assays based on the use of replicatable hybridization probes. Clin Chem. 1989 Sep;35(9):1826–1831. [PubMed] [Google Scholar]
  100. Lomeli H., Tyagi S., Pritchard C. G., Lizardi P. M., Kramer F. R. Quantitative assays based on the use of replicatable hybridization probes. Clin Chem. 1989 Sep;35(9):1826–1831. [PubMed] [Google Scholar]
  101. Lüneberg E., Jensen J. S., Frosch M. Detection of Mycoplasma pneumoniae by polymerase chain reaction and nonradioactive hybridization in microtiter plates. J Clin Microbiol. 1993 May;31(5):1088–1094. doi: 10.1128/jcm.31.5.1088-1094.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  102. Maass M., Dalhoff K. Comparison of sample preparation methods for detection of Chlamydia pneumoniae in bronchoalveolar lavage fluid by PCR. J Clin Microbiol. 1994 Oct;32(10):2616–2619. doi: 10.1128/jcm.32.10.2616-2619.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  103. Maiwald M., Kissel K., Srimuang S., von Knebel Doeberitz M., Sonntag H. G. Comparison of polymerase chain reaction and conventional culture for the detection of legionellas in hospital water samples. J Appl Bacteriol. 1994 Mar;76(3):216–225. doi: 10.1111/j.1365-2672.1994.tb01619.x. [DOI] [PubMed] [Google Scholar]
  104. Maiwald M., Schill M., Stockinger C., Helbig J. H., Lück P. C., Witzleb W., Sonntag H. G. Detection of Legionella DNA in human and guinea pig urine samples by the polymerase chain reaction. Eur J Clin Microbiol Infect Dis. 1995 Jan;14(1):25–33. doi: 10.1007/BF02112614. [DOI] [PubMed] [Google Scholar]
  105. Makimura K., Murayama S. Y., Yamaguchi H. Detection of a wide range of medically important fungi by the polymerase chain reaction. J Med Microbiol. 1994 May;40(5):358–364. doi: 10.1099/00222615-40-5-358. [DOI] [PubMed] [Google Scholar]
  106. Mangiapan G., Vokurka M., Schouls L., Cadranel J., Lecossier D., van Embden J., Hance A. J. Sequence capture-PCR improves detection of mycobacterial DNA in clinical specimens. J Clin Microbiol. 1996 May;34(5):1209–1215. doi: 10.1128/jcm.34.5.1209-1215.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  107. Marmion B. P., Williamson J., Worswick D. A., Kok T. W., Harris R. J. Experience with newer techniques for the laboratory detection of Mycoplasma pneumoniae infection: Adelaide, 1978-1992. Clin Infect Dis. 1993 Aug;17 (Suppl 1):S90–S99. doi: 10.1093/clinids/17.supplement_1.s90. [DOI] [PubMed] [Google Scholar]
  108. Meade B. D., Bollen A. Recommendations for use of the polymerase chain reaction in the diagnosis of Bordetella pertussis infections. J Med Microbiol. 1994 Jul;41(1):51–55. doi: 10.1099/00222615-41-1-51. [DOI] [PubMed] [Google Scholar]
  109. Melchers W. J., Verweij P. E., van den Hurk P., van Belkum A., De Pauw B. E., Hoogkamp-Korstanje J. A., Meis J. F. General primer-mediated PCR for detection of Aspergillus species. J Clin Microbiol. 1994 Jul;32(7):1710–1717. doi: 10.1128/jcm.32.7.1710-1717.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  110. Miller L. A., Beebe J. L., Butler J. C., Martin W., Benson R., Hoffman R. E., Fields B. S. Use of polymerase chain reaction in an epidemiologic investigation of Pontiac fever. J Infect Dis. 1993 Sep;168(3):769–772. doi: 10.1093/infdis/168.3.769. [DOI] [PubMed] [Google Scholar]
  111. Miller N., Hernandez S. G., Cleary T. J. Evaluation of Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test and PCR for direct detection of Mycobacterium tuberculosis in clinical specimens. J Clin Microbiol. 1994 Feb;32(2):393–397. doi: 10.1128/jcm.32.2.393-397.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  112. Miyakawa Y., Mabuchi T., Fukazawa Y. New method for detection of Candida albicans in human blood by polymerase chain reaction. J Clin Microbiol. 1993 Dec;31(12):3344–3347. doi: 10.1128/jcm.31.12.3344-3347.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  113. Miyakawa Y., Mabuchi T., Kagaya K., Fukazawa Y. Isolation and characterization of a species-specific DNA fragment for detection of Candida albicans by polymerase chain reaction. J Clin Microbiol. 1992 Apr;30(4):894–900. doi: 10.1128/jcm.30.4.894-900.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  114. Miyazaki Y., Koga H., Kohno S., Kaku M. Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples. J Clin Microbiol. 1993 Aug;31(8):2228–2232. doi: 10.1128/jcm.31.8.2228-2232.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  115. Moore D. F., Curry J. I. Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by Amplicor PCR. J Clin Microbiol. 1995 Oct;33(10):2686–2691. doi: 10.1128/jcm.33.10.2686-2691.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  116. Mori J., Clewley J. P. Polymerase chain reaction and sequencing for typing rhinovirus RNA. J Med Virol. 1994 Dec;44(4):323–329. doi: 10.1002/jmv.1890440403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  117. Mullis K., Faloona F., Scharf S., Saiki R., Horn G., Erlich H. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb Symp Quant Biol. 1986;51(Pt 1):263–273. doi: 10.1101/sqb.1986.051.01.032. [DOI] [PubMed] [Google Scholar]
  118. Myint S., Johnston S., Sanderson G., Simpson H. Evaluation of nested polymerase chain methods for the detection of human coronaviruses 229E and OC43. Mol Cell Probes. 1994 Oct;8(5):357–364. doi: 10.1006/mcpr.1994.1052. [DOI] [PMC free article] [PubMed] [Google Scholar]
  119. Nakamura H., Shibata Y., Kudo Y., Saito S., Kimura H., Tomoike H. [Detection of Aspergillus fumigatus DNA by polymerase chain reaction in the clinical samples from individuals with pulmonary aspergillosis]. Rinsho Byori. 1994 Jul;42(7):676–681. [PubMed] [Google Scholar]
  120. Nichol S. T., Spiropoulou C. F., Morzunov S., Rollin P. E., Ksiazek T. G., Feldmann H., Sanchez A., Childs J., Zaki S., Peters C. J. Genetic identification of a hantavirus associated with an outbreak of acute respiratory illness. Science. 1993 Nov 5;262(5135):914–917. doi: 10.1126/science.8235615. [DOI] [PubMed] [Google Scholar]
  121. Nicholson K. G., Kent J., Ireland D. C. Respiratory viruses and exacerbations of asthma in adults. BMJ. 1993 Oct 16;307(6910):982–986. doi: 10.1136/bmj.307.6910.982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  122. Nolte F. S., Metchock B., McGowan J. E., Jr, Edwards A., Okwumabua O., Thurmond C., Mitchell P. S., Plikaytis B., Shinnick T. Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction and DNA hybridization. J Clin Microbiol. 1993 Jul;31(7):1777–1782. doi: 10.1128/jcm.31.7.1777-1782.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  123. Noordhoek G. T., Kaan J. A., Mulder S., Wilke H., Kolk A. H. Routine application of the polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples. J Clin Pathol. 1995 Sep;48(9):810–814. doi: 10.1136/jcp.48.9.810. [DOI] [PMC free article] [PubMed] [Google Scholar]
  124. Noordhoek G. T., Kolk A. H., Bjune G., Catty D., Dale J. W., Fine P. E., Godfrey-Faussett P., Cho S. N., Shinnick T., Svenson S. B. Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis: a blind comparison study among seven laboratories. J Clin Microbiol. 1994 Feb;32(2):277–284. doi: 10.1128/jcm.32.2.277-284.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  125. Noordhoek G. T., van Embden J. D., Kolk A. H. Reliability of nucleic acid amplification for detection of Mycobacterium tuberculosis: an international collaborative quality control study among 30 laboratories. J Clin Microbiol. 1996 Oct;34(10):2522–2525. doi: 10.1128/jcm.34.10.2522-2525.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  126. Olive D. M., Al-Mufti S., Al-Mulla W., Khan M. A., Pasca A., Stanway G., Al-Nakib W. Detection and differentiation of picornaviruses in clinical samples following genomic amplification. J Gen Virol. 1990 Sep;71(Pt 9):2141–2147. doi: 10.1099/0022-1317-71-9-2141. [DOI] [PubMed] [Google Scholar]
  127. Olsson M., Elvin K., Löfdahl S., Linder E. Detection of Pneumocystis carinii DNA in sputum and bronchoalveolar lavage samples by polymerase chain reaction. J Clin Microbiol. 1993 Feb;31(2):221–226. doi: 10.1128/jcm.31.2.221-226.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  128. Ouchi K., Nakazawa T., Karita M., Kanehara Y. Prevalence of Chlamydia pneumoniae in acute lower respiratory infection in the pediatric population in Japan. Acta Paediatr Jpn. 1994 Jun;36(3):256–260. doi: 10.1111/j.1442-200x.1994.tb03174.x. [DOI] [PubMed] [Google Scholar]
  129. Pao C. C., Yen T. S., You J. B., Maa J. S., Fiss E. H., Chang C. H. Detection and identification of Mycobacterium tuberculosis by DNA amplification. J Clin Microbiol. 1990 Sep;28(9):1877–1880. doi: 10.1128/jcm.28.9.1877-1880.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  130. Paton A. W., Paton J. C., Lawrence A. J., Goldwater P. N., Harris R. J. Rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by reverse transcription and polymerase chain reaction amplification. J Clin Microbiol. 1992 Apr;30(4):901–904. doi: 10.1128/jcm.30.4.901-904.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  131. Pattyn S. R., Provinciael D., Lambrechts R., Ceuppens P. Rapid diagnosis of viral respiratory infections. Comparison between immunofluorescence on clinical samples and immunofluorescence on centrifuged cell cultures. Acta Clin Belg. 1991;46(1):7–12. doi: 10.1080/17843286.1991.11718134. [DOI] [PubMed] [Google Scholar]
  132. Pattyn S. R., Ursi D., Ieven M., Raes V., Jamet P. Polymerase chain reaction amplifying DNA coding for species-specific rRNA of Mycobacterium leprae. Int J Lepr Other Mycobact Dis. 1992 Jun;60(2):234–243. [PubMed] [Google Scholar]
  133. Pfaller M. A. Application of new technology to the detection, identification, and antimicrobial susceptibility testing of mycobacteria. Am J Clin Pathol. 1994 Mar;101(3):329–337. doi: 10.1093/ajcp/101.3.329. [DOI] [PubMed] [Google Scholar]
  134. Pfyffer G. E., Kissling P., Wirth R., Weber R. Direct detection of Mycobacterium tuberculosis complex in respiratory specimens by a target-amplified test system. J Clin Microbiol. 1994 Apr;32(4):918–923. doi: 10.1128/jcm.32.4.918-923.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  135. Piatak M., Jr, Luk K. C., Williams B., Lifson J. D. Quantitative competitive polymerase chain reaction for accurate quantitation of HIV DNA and RNA species. Biotechniques. 1993 Jan;14(1):70–81. [PubMed] [Google Scholar]
  136. Pierre C., Lecossier D., Boussougant Y., Bocart D., Joly V., Yeni P., Hance A. J. Use of a reamplification protocol improves sensitivity of detection of Mycobacterium tuberculosis in clinical samples by amplification of DNA. J Clin Microbiol. 1991 Apr;29(4):712–717. doi: 10.1128/jcm.29.4.712-717.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  137. Pierre C., Olivier C., Lecossier D., Boussougant Y., Yeni P., Hance A. J. Diagnosis of primary tuberculosis in children by amplification and detection of mycobacterial DNA. Am Rev Respir Dis. 1993 Feb;147(2):420–424. doi: 10.1164/ajrccm/147.2.420. [DOI] [PubMed] [Google Scholar]
  138. Polanco A. M., Rodríguez-Tudela J. L., Martínez-Suárez J. V. Detection of pathogenic fungi in human blood by the polymerase chain reaction. Eur J Clin Microbiol Infect Dis. 1995 Jul;14(7):618–621. doi: 10.1007/BF01690738. [DOI] [PubMed] [Google Scholar]
  139. Polanco A. M., Rodríguez-Tudela J. L., Martínez-Suárez J. V. Detection of pathogenic fungi in human blood by the polymerase chain reaction. Eur J Clin Microbiol Infect Dis. 1995 Jul;14(7):618–621. doi: 10.1007/BF01690738. [DOI] [PubMed] [Google Scholar]
  140. Portaels F., Serruys E., De Beenhouwer H., Degraux J., De Ridder K., Fissette K., Gomez-Marin J., Goossens H., Mühlberger F., Pattyn S. R. Evaluation of the Gen-Probe amplified Mycobacterium tuberculosis direct test for the routine diagnosis of pulmonary tuberculosis. Acta Clin Belg. 1996;51(3):144–149. doi: 10.1080/17843286.1996.11718502. [DOI] [PubMed] [Google Scholar]
  141. Prückl P. M., Aspöck C., Makristathis A., Rotter M. L., Wank H., Willinger B., Hirschl A. M. Polymerase chain reaction for detection of Chlamydia pneumoniae in gargled-water specimens of children. Eur J Clin Microbiol Infect Dis. 1995 Feb;14(2):141–144. doi: 10.1007/BF02111875. [DOI] [PubMed] [Google Scholar]
  142. Rand K. H., Houck H., Wolff M. Detection of candidemia by polymerase chain reaction. Mol Cell Probes. 1994 Jun;8(3):215–221. doi: 10.1006/mcpr.1994.1030. [DOI] [PubMed] [Google Scholar]
  143. Raoult D., Vestris G., Enea M. Isolation of 16 strains of Coxiella burnetii from patients by using a sensitive centrifugation cell culture system and establishment of the strains in HEL cells. J Clin Microbiol. 1990 Nov;28(11):2482–2484. doi: 10.1128/jcm.28.11.2482-2484.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  144. Rasmussen S. J., Douglas F. P., Timms P. PCR detection and differentiation of Chlamydia pneumoniae, Chlamydia psittaci and Chlamydia trachomatis. Mol Cell Probes. 1992 Oct;6(5):389–394. doi: 10.1016/0890-8508(92)90032-s. [DOI] [PubMed] [Google Scholar]
  145. Reddy L. V., Kumar A., Kurup V. P. Specific amplification of Aspergillus fumigatus DNA by polymerase chain reaction. Mol Cell Probes. 1993 Apr;7(2):121–126. doi: 10.1006/mcpr.1993.1016. [DOI] [PubMed] [Google Scholar]
  146. Reiss E., Morrison C. J. Nonculture methods for diagnosis of disseminated candidiasis. Clin Microbiol Rev. 1993 Oct;6(4):311–323. doi: 10.1128/cmr.6.4.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  147. Reznikov M., Blackmore T. K., Finlay-Jones J. J., Gordon D. L. Comparison of nasopharyngeal aspirates and throat swab specimens in a polymerase chain reaction-based test for Mycoplasma pneumoniae. Eur J Clin Microbiol Infect Dis. 1995 Jan;14(1):58–61. doi: 10.1007/BF02112622. [DOI] [PubMed] [Google Scholar]
  148. Rolfs A., Beige J., Finckh U., Köhler B., Schaberg T., Lokies J., Lode H. Amplification of Mycobacterium tuberculosis from peripheral blood. J Clin Microbiol. 1995 Dec;33(12):3312–3314. doi: 10.1128/jcm.33.12.3312-3314.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  149. Roure C. Le programme régional de vaccination en Europe (1991-1993). Sante. 1994 May-Jun;4(3):145–150. [PubMed] [Google Scholar]
  150. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  151. Scadden D. T., Wang Z., Groopman J. E. Quantitation of plasma human immunodeficiency virus type 1 RNA by competitive polymerase chain reaction. J Infect Dis. 1992 Jun;165(6):1119–1123. doi: 10.1093/infdis/165.6.1119. [DOI] [PubMed] [Google Scholar]
  152. Schirm J., Oostendorp L. A., Mulder J. G. Comparison of Amplicor, in-house PCR, and conventional culture for detection of Mycobacterium tuberculosis in clinical samples. J Clin Microbiol. 1995 Dec;33(12):3221–3224. doi: 10.1128/jcm.33.12.3221-3224.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  153. Schluger N. W., Condos R., Lewis S., Rom W. N. Amplification of DNA of Mycobacterium tuberculosis from peripheral blood of patients with pulmonary tuberculosis. Lancet. 1994 Jul 23;344(8917):232–233. doi: 10.1016/s0140-6736(94)92999-8. [DOI] [PubMed] [Google Scholar]
  154. Schluger N. W., Kinney D., Harkin T. J., Rom W. N. Clinical utility of the polymerase chain reaction in the diagnosis of infections due to Mycobacterium tuberculosis. Chest. 1994 Apr;105(4):1116–1121. doi: 10.1378/chest.105.4.1116. [DOI] [PubMed] [Google Scholar]
  155. Schläpfer G., Cherry J. D., Heininger U., Uberall M., Schmitt-Grohé S., Laussucq S., Just M., Stehr K. Polymerase chain reaction identification of Bordetella pertussis infections in vaccinees and family members in a pertussis vaccine efficacy trial in Germany. Pediatr Infect Dis J. 1995 Mar;14(3):209–214. doi: 10.1097/00006454-199503000-00008. [DOI] [PubMed] [Google Scholar]
  156. Schläpfer G., Senn H. P., Berger R., Just M. Use of the polymerase chain reaction to detect Bordetella pertussis in patients with mild or atypical symptoms of infection. Eur J Clin Microbiol Infect Dis. 1993 Jun;12(6):459–463. doi: 10.1007/BF01967443. [DOI] [PubMed] [Google Scholar]
  157. Shah J. S., Liu J., Buxton D., Hendricks A., Robinson L., Radcliffe G., King W., Lane D., Olive D. M., Klinger J. D. Q-beta replicase-amplified assay for detection of Mycobacterium tuberculosis directly from clinical specimens. J Clin Microbiol. 1995 Jun;33(6):1435–1441. doi: 10.1128/jcm.33.6.1435-1441.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  158. Shawar R. M., el-Zaatari F. A., Nataraj A., Clarridge J. E. Detection of Mycobacterium tuberculosis in clinical samples by two-step polymerase chain reaction and nonisotopic hybridization methods. J Clin Microbiol. 1993 Jan;31(1):61–65. doi: 10.1128/jcm.31.1.61-65.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  159. Skakni L., Sardet A., Just J., Landman-Parker J., Costil J., Moniot-Ville N., Bricout F., Garbarg-Chenon A. Detection of Mycoplasma pneumoniae in clinical samples from pediatric patients by polymerase chain reaction. J Clin Microbiol. 1992 Oct;30(10):2638–2643. doi: 10.1128/jcm.30.10.2638-2643.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  160. Soini H., Skurnik M., Liippo K., Tala E., Viljanen M. K. Detection and identification of mycobacteria by amplification of a segment of the gene coding for the 32-kilodalton protein. J Clin Microbiol. 1992 Aug;30(8):2025–2028. doi: 10.1128/jcm.30.8.2025-2028.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  161. Spreadbury C., Holden D., Aufauvre-Brown A., Bainbridge B., Cohen J. Detection of Aspergillus fumigatus by polymerase chain reaction. J Clin Microbiol. 1993 Mar;31(3):615–621. doi: 10.1128/jcm.31.3.615-621.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  162. Stein A., Raoult D. Detection of Coxiella burnetti by DNA amplification using polymerase chain reaction. J Clin Microbiol. 1992 Sep;30(9):2462–2466. doi: 10.1128/jcm.30.9.2462-2466.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  163. Strebel P. M., Cochi S. L., Farizo K. M., Payne B. J., Hanauer S. D., Baughman A. L. Pertussis in Missouri: evaluation of nasopharyngeal culture, direct fluorescent antibody testing, and clinical case definitions in the diagnosis of pertussis. Clin Infect Dis. 1993 Feb;16(2):276–285. doi: 10.1093/clind/16.2.276. [DOI] [PubMed] [Google Scholar]
  164. Takewaki S., Okuzumi K., Ishiko H., Nakahara K., Ohkubo A., Nagai R. Genus-specific polymerase chain reaction for the mycobacterial dnaJ gene and species-specific oligonucleotide probes. J Clin Microbiol. 1993 Feb;31(2):446–450. doi: 10.1128/jcm.31.2.446-450.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  165. Takewaki S., Okuzumi K., Manabe I., Tanimura M., Miyamura K., Nakahara K., Yazaki Y., Ohkubo A., Nagai R. Nucleotide sequence comparison of the mycobacterial dnaJ gene and PCR-restriction fragment length polymorphism analysis for identification of mycobacterial species. Int J Syst Bacteriol. 1994 Jan;44(1):159–166. doi: 10.1099/00207713-44-1-159. [DOI] [PubMed] [Google Scholar]
  166. Tamburrini E., Mencarini P., De Luca A., Maiuro G., Ventura G., Antinori A., Ammassari A., Visconti E., Ortona L., Siracusano A. Diagnosis of Pneumocystis carinii pneumonia: specificity and sensitivity of polymerase chain reaction in comparison with immunofluorescence in bronchoalveolar lavage specimens. J Med Microbiol. 1993 Jun;38(6):449–453. doi: 10.1099/00222615-38-6-449. [DOI] [PubMed] [Google Scholar]
  167. Tang C. M., Holden D. W., Aufauvre-Brown A., Cohen J. The detection of Aspergillus spp. by the polymerase chain reaction and its evaluation in bronchoalveolar lavage fluid. Am Rev Respir Dis. 1993 Nov;148(5):1313–1317. doi: 10.1164/ajrccm/148.5.1313. [DOI] [PubMed] [Google Scholar]
  168. Taranger J., Trollfors B., Lind L., Zackrisson G., Beling-Holmquist K. Environmental contamination leading to false-positive polymerase chain reaction for pertussis. Pediatr Infect Dis J. 1994 Oct;13(10):936–937. doi: 10.1097/00006454-199410000-00020. [DOI] [PubMed] [Google Scholar]
  169. Telenti A., Imboden P., Germann D. Competitive polymerase chain reaction using an internal standard: application to the quantitation of viral DNA. J Virol Methods. 1992 Sep;39(3):259–268. doi: 10.1016/0166-0934(92)90099-y. [DOI] [PubMed] [Google Scholar]
  170. Telenti A., Imboden P., Marchesi F., Lowrie D., Cole S., Colston M. J., Matter L., Schopfer K., Bodmer T. Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis. Lancet. 1993 Mar 13;341(8846):647–650. doi: 10.1016/0140-6736(93)90417-f. [DOI] [PubMed] [Google Scholar]
  171. Telenti A., Imboden P., Marchesi F., Schmidheini T., Bodmer T. Direct, automated detection of rifampin-resistant Mycobacterium tuberculosis by polymerase chain reaction and single-strand conformation polymorphism analysis. Antimicrob Agents Chemother. 1993 Oct;37(10):2054–2058. doi: 10.1128/aac.37.10.2054. [DOI] [PMC free article] [PubMed] [Google Scholar]
  172. Thom D. H., Grayston J. T., Campbell L. A., Kuo C. C., Diwan V. K., Wang S. P. Respiratory infection with Chlamydia pneumoniae in middle-aged and older adult outpatients. Eur J Clin Microbiol Infect Dis. 1994 Oct;13(10):785–792. doi: 10.1007/BF02111337. [DOI] [PubMed] [Google Scholar]
  173. Tjhie J. H., van Kuppeveld F. J., Roosendaal R., Melchers W. J., Gordijn R., MacLaren D. M., Walboomers J. M., Meijer C. J., van den Brule A. J. Direct PCR enables detection of Mycoplasma pneumoniae in patients with respiratory tract infections. J Clin Microbiol. 1994 Jan;32(1):11–16. doi: 10.1128/jcm.32.1.11-16.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  174. To H., Kako N., Zhang G. Q., Otsuka H., Ogawa M., Ochiai O., Nguyen S. V., Yamaguchi T., Fukushi H., Nagaoka N. Q fever pneumonia in children in Japan. J Clin Microbiol. 1996 Mar;34(3):647–651. doi: 10.1128/jcm.34.3.647-651.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  175. Tong C. Y., Sillis M. Detection of Chlamydia pneumoniae and Chlamydia psittaci in sputum samples by PCR. J Clin Pathol. 1993 Apr;46(4):313–317. doi: 10.1136/jcp.46.4.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  176. Torgersen H., Skern T., Blaas D. Typing of human rhinoviruses based on sequence variations in the 5' non-coding region. J Gen Virol. 1989 Nov;70(Pt 11):3111–3116. doi: 10.1099/0022-1317-70-11-3111. [DOI] [PubMed] [Google Scholar]
  177. Ulrich P. P., Romeo J. M., Daniel L. J., Vyas G. N. An improved method for the detection of hepatitis C virus RNA in plasma utilizing heminested primers and internal control RNA. PCR Methods Appl. 1993 Feb;2(3):241–249. doi: 10.1101/gr.2.3.241. [DOI] [PubMed] [Google Scholar]
  178. Ursi J. P., Ursi D., Ieven M., Pattyn S. R. Utility of an internal control for the polymerase chain reaction. Application to detection of Mycoplasma pneumoniae in clinical specimens. APMIS. 1992 Jul;100(7):635–639. doi: 10.1111/j.1699-0463.1992.tb03978.x. [DOI] [PubMed] [Google Scholar]
  179. Vlaspolder F., Singer P., Roggeveen C. Diagnostic value of an amplification method (Gen-Probe) compared with that of culture for diagnosis of tuberculosis. J Clin Microbiol. 1995 Oct;33(10):2699–2703. doi: 10.1128/jcm.33.10.2699-2703.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  180. Vuorinen P., Miettinen A., Vuento R., Hällström O. Direct detection of Mycobacterium tuberculosis complex in respiratory specimens by Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test and Roche Amplicor Mycobacterium Tuberculosis Test. J Clin Microbiol. 1995 Jul;33(7):1856–1859. doi: 10.1128/jcm.33.7.1856-1859.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  181. Wadowsky R. M., Laus S., Libert T., States S. J., Ehrlich G. D. Inhibition of PCR-based assay for Bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab. J Clin Microbiol. 1994 Apr;32(4):1054–1057. doi: 10.1128/jcm.32.4.1054-1057.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  182. Wadowsky R. M., Yee R. B., Mezmar L., Wing E. J., Dowling J. N. Hot water systems as sources of Legionella pneumophila in hospital and nonhospital plumbing fixtures. Appl Environ Microbiol. 1982 May;43(5):1104–1110. doi: 10.1128/aem.43.5.1104-1110.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  183. Walker D. A., Taylor I. K., Mitchell D. M., Shaw R. J. Comparison of polymerase chain reaction amplification of two mycobacterial DNA sequences, IS6110 and the 65kDa antigen gene, in the diagnosis of tuberculosis. Thorax. 1992 Sep;47(9):690–694. doi: 10.1136/thx.47.9.690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  184. Walker G. T., Nadeau J. G., Spears P. A., Schram J. L., Nycz C. M., Shank D. D. Multiplex strand displacement amplification (SDA) and detection of DNA sequences from Mycobacterium tuberculosis and other mycobacteria. Nucleic Acids Res. 1994 Jul 11;22(13):2670–2677. doi: 10.1093/nar/22.13.2670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  185. Watson M. W., Lambden P. R., Clarke I. N. Genetic diversity and identification of human infection by amplification of the chlamydial 60-kilodalton cysteine-rich outer membrane protein gene. J Clin Microbiol. 1991 Jun;29(6):1188–1193. doi: 10.1128/jcm.29.6.1188-1193.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  186. Whelen A. C., Felmlee T. A., Hunt J. M., Williams D. L., Roberts G. D., Stockman L., Persing D. H. Direct genotypic detection of Mycobacterium tuberculosis rifampin resistance in clinical specimens by using single-tube heminested PCR. J Clin Microbiol. 1995 Mar;33(3):556–561. doi: 10.1128/jcm.33.3.556-561.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  187. Williams D. L., Waguespack C., Eisenach K., Crawford J. T., Portaels F., Salfinger M., Nolan C. M., Abe C., Sticht-Groh V., Gillis T. P. Characterization of rifampin-resistance in pathogenic mycobacteria. Antimicrob Agents Chemother. 1994 Oct;38(10):2380–2386. doi: 10.1128/aac.38.10.2380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  188. Wilson S. M., McNerney R., Nye P. M., Godfrey-Faussett P. D., Stoker N. G., Voller A. Progress toward a simplified polymerase chain reaction and its application to diagnosis of tuberculosis. J Clin Microbiol. 1993 Apr;31(4):776–782. doi: 10.1128/jcm.31.4.776-782.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  189. Wobeser W. L., Krajden M., Conly J., Simpson H., Yim B., D'costa M., Fuksa M., Hian-Cheong C., Patterson M., Phillips A. Evaluation of Roche Amplicor PCR assay for Mycobacterium tuberculosis. J Clin Microbiol. 1996 Jan;34(1):134–139. doi: 10.1128/jcm.34.1.134-139.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  190. Wolcott M. J. Advances in nucleic acid-based detection methods. Clin Microbiol Rev. 1992 Oct;5(4):370–386. doi: 10.1128/cmr.5.4.370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  191. Wu D. Y., Wallace R. B. The ligation amplification reaction (LAR)--amplification of specific DNA sequences using sequential rounds of template-dependent ligation. Genomics. 1989 May;4(4):560–569. doi: 10.1016/0888-7543(89)90280-2. [DOI] [PubMed] [Google Scholar]
  192. Yajko D. M., Wagner C., Tevere V. J., Kocagöz T., Hadley W. K., Chambers H. F. Quantitative culture of Mycobacterium tuberculosis from clinical sputum specimens and dilution endpoint of its detection by the Amplicor PCR assay. J Clin Microbiol. 1995 Jul;33(7):1944–1947. doi: 10.1128/jcm.33.7.1944-1947.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  193. Yuen K. Y., Chan K. S., Chan C. M., Ho B. S., Dai L. K., Chau P. Y., Ng M. H. Use of PCR in routine diagnosis of treated and untreated pulmonary tuberculosis. J Clin Pathol. 1993 Apr;46(4):318–322. doi: 10.1136/jcp.46.4.318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  194. Yuen L. K., Ross B. C., Jackson K. M., Dwyer B. Characterization of Mycobacterium tuberculosis strains from Vietnamese patients by Southern blot hybridization. J Clin Microbiol. 1993 Jun;31(6):1615–1618. doi: 10.1128/jcm.31.6.1615-1618.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  195. Zambardi G., Roure C., Boujaafar N., Fouqué B., Freney J., Fleurette J. Comparison of three primer sets for the detection of Mycobacterium tuberculosis in clinical samples by polymerase chain reaction. Ann Biol Clin (Paris) 1993;51(10-11):893–897. [PubMed] [Google Scholar]
  196. al-Nakib W., Higgins P. G., Barrow G. I., Tyrrell D. A., Andries K., Vanden Bussche G., Taylor N., Janssen P. A. Suppression of colds in human volunteers challenged with rhinovirus by a new synthetic drug (R61837). Antimicrob Agents Chemother. 1989 Apr;33(4):522–525. doi: 10.1128/aac.33.4.522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  197. de Lamballerie X., Zandotti C., Vignoli C., Bollet C., de Micco P. A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification. Res Microbiol. 1992 Oct;143(8):785–790. doi: 10.1016/0923-2508(92)90107-y. [DOI] [PubMed] [Google Scholar]
  198. de Lassence A., Lecossier D., Pierre C., Cadranel J., Stern M., Hance A. J. Detection of mycobacterial DNA in pleural fluid from patients with tuberculous pleurisy by means of the polymerase chain reaction: comparison of two protocols. Thorax. 1992 Apr;47(4):265–269. doi: 10.1136/thx.47.4.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  199. de Wit D., Wootton M., Dhillon J., Mitchison D. A. The bacterial DNA content of mouse organs in the Cornell model of dormant tuberculosis. Tuber Lung Dis. 1995 Dec;76(6):555–562. doi: 10.1016/0962-8479(95)90534-0. [DOI] [PubMed] [Google Scholar]
  200. deWit D., Wootton M., Allan B., Steyn L. Simple method for production of internal control DNA for Mycobacterium tuberculosis polymerase chain reaction assays. J Clin Microbiol. 1993 Aug;31(8):2204–2207. doi: 10.1128/jcm.31.8.2204-2207.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  201. van Deventer A. J., Goessens W. H., van Belkum A., van Vliet H. J., van Etten E. W., Verbrugh H. A. Improved detection of Candida albicans by PCR in blood of neutropenic mice with systemic candidiasis. J Clin Microbiol. 1995 Mar;33(3):625–628. doi: 10.1128/jcm.33.3.625-628.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  202. van Kuppeveld F. J., Johansson K. E., Galama J. M., Kissing J., Bölske G., Hjelm E., van der Logt J. T., Melchers W. J. 16S rRNA based polymerase chain reaction compared with culture and serological methods for diagnosis of Mycoplasma pneumoniae infection. Eur J Clin Microbiol Infect Dis. 1994 May;13(5):401–405. doi: 10.1007/BF01971997. [DOI] [PubMed] [Google Scholar]
  203. van Soolingen D., de Haas P. E., Hermans P. W., Groenen P. M., van Embden J. D. Comparison of various repetitive DNA elements as genetic markers for strain differentiation and epidemiology of Mycobacterium tuberculosis. J Clin Microbiol. 1993 Aug;31(8):1987–1995. doi: 10.1128/jcm.31.8.1987-1995.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  204. van der Zee A., Agterberg C., Peeters M., Schellekens J., Mooi F. R. Polymerase chain reaction assay for pertussis: simultaneous detection and discrimination of Bordetella pertussis and Bordetella parapertussis. J Clin Microbiol. 1993 Aug;31(8):2134–2140. doi: 10.1128/jcm.31.8.2134-2140.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  205. von Eiff M., Roos N., Fegeler W., von Eiff C., Zühlsdorf M., Glaser J., van de Loo J. Pulmonary fungal infections in immunocompromised patients: incidence and risk factors. Mycoses. 1994 Sep-Oct;37(9-10):329–335. doi: 10.1111/myc.1994.37.9-10.329. [DOI] [PubMed] [Google Scholar]

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