Figure 1  .

(A) Nested enteroviral reverse transcription polymerase chain reaction (PCR), performed on left ventricular and septal tissue obtained from explanted hearts and biopsies taken from patients with familial early dilated cardiomyopathy. Representative data are shown in lanes 9 to 14. Other lanes are: culture grown coxsackie virus B3 (CVB3; lane 2), CVB3 infected mouse heart (lane 3), 5 and 0.5 copies of pBlue script plasmid carrying CVB3 cDNA (lanes 4 and 5, respectively), and CVB3 infected human hearts (lanes 15 and 16); molecular weight markers are shown in the first and last lanes; reagent contamination controls from first and second rounds of PCR amplification are shown in lanes 7 and 8, respectively; an uninfected mouse heart used as negative control is shown in lane 6. (B) Amplification of the myoglobin housekeeping gene following reverse transcription. The order of samples as for panel (A).