Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1995 Jan;63(1):363–365. doi: 10.1128/iai.63.1.363-365.1995

Immunoreactivity and bioactivity of lipopolysaccharide-binding protein in normal and heat-inactivated sera.

K Mészáros 1, S Aberle 1, M White 1, J B Parent 1
PMCID: PMC173003  PMID: 7806380

Abstract

The lipopolysaccharide (LPS)-potentiating effect of serum is due to LPS-binding protein (LBP), which facilitates the binding of LPS to CD14 receptors. We observed a remarkable heat sensitivity of recombinant LBP and various sera with respect to both immunoreactivity (measured by enzyme-linked immunosorbent assay) and bioactivity (potentiation of LPS induction of tumor necrosis factor in monocytes). Human sera were more active and more heat sensitive than fetal bovine sera. The commonly practiced heat inactivation of human serum (56 degrees C, 30 min) resulted in a 70% loss of bioactivity, which caused an apparent decrease in the potency of LPS.

Full Text

The Full Text of this article is available as a PDF (170.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Danner R. L., Elin R. J., Hosseini J. M., Wesley R. A., Reilly J. M., Parillo J. E. Endotoxemia in human septic shock. Chest. 1991 Jan;99(1):169–175. doi: 10.1378/chest.99.1.169. [DOI] [PubMed] [Google Scholar]
  2. Fang I. S., Wisniewski M. A., Huntenburg C. C., Knight L. S., Bubbers J. E., Schneidkraut M. J. Inhibition of lipopolysaccharide-associated endotoxin activities in vitro and in vivo by the human anti-lipid A monoclonal antibody SdJ5-1.17.15. Infect Immun. 1993 Sep;61(9):3873–3878. doi: 10.1128/iai.61.9.3873-3878.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fujihara M., Connolly N., Ito N., Suzuki T. Properties of protein kinase C isoforms (beta II, epsilon, and zeta) in a macrophage cell line (J774) and their roles in LPS-induced nitric oxide production. J Immunol. 1994 Feb 15;152(4):1898–1906. [PubMed] [Google Scholar]
  4. Gallay P., Carrel S., Glauser M. P., Barras C., Ulevitch R. J., Tobias P. S., Baumgartner J. D., Heumann D. Purification and characterization of murine lipopolysaccharide-binding protein. Infect Immun. 1993 Feb;61(2):378–383. doi: 10.1128/iai.61.2.378-383.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gallay P., Heumann D., Le Roy D., Barras C., Glauser M. P. Lipopolysaccharide-binding protein as a major plasma protein responsible for endotoxemic shock. Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):9935–9938. doi: 10.1073/pnas.90.21.9935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hailman E., Lichenstein H. S., Wurfel M. M., Miller D. S., Johnson D. A., Kelley M., Busse L. A., Zukowski M. M., Wright S. D. Lipopolysaccharide (LPS)-binding protein accelerates the binding of LPS to CD14. J Exp Med. 1994 Jan 1;179(1):269–277. doi: 10.1084/jem.179.1.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hamilton J. A., Whitty G. A., Wojta J., Gallichio M., McGrath K., Ianches G. Regulation of plasminogen activator inhibitor-1 levels in human monocytes. Cell Immunol. 1993 Nov;152(1):7–17. doi: 10.1006/cimm.1993.1263. [DOI] [PubMed] [Google Scholar]
  8. Han J., Lee J. D., Tobias P. S., Ulevitch R. J. Endotoxin induces rapid protein tyrosine phosphorylation in 70Z/3 cells expressing CD14. J Biol Chem. 1993 Nov 25;268(33):25009–25014. [PubMed] [Google Scholar]
  9. Heumann D., Gallay P., Barras C., Zaech P., Ulevitch R. J., Tobias P. S., Glauser M. P., Baumgartner J. D. Control of lipopolysaccharide (LPS) binding and LPS-induced tumor necrosis factor secretion in human peripheral blood monocytes. J Immunol. 1992 Jun 1;148(11):3505–3512. [PubMed] [Google Scholar]
  10. Kirkland T. N., Finley F., Leturcq D., Moriarty A., Lee J. D., Ulevitch R. J., Tobias P. S. Analysis of lipopolysaccharide binding by CD14. J Biol Chem. 1993 Nov 25;268(33):24818–24823. [PubMed] [Google Scholar]
  11. Mathison J. C., Tobias P. S., Wolfson E., Ulevitch R. J. Plasma lipopolysaccharide (LPS)-binding protein. A key component in macrophage recognition of gram-negative LPS. J Immunol. 1992 Jul 1;149(1):200–206. [PubMed] [Google Scholar]
  12. Mészáros K., Aberle S., Dedrick R., Machovich R., Horwitz A., Birr C., Theofan G., Parent J. B. Monocyte tissue factor induction by lipopolysaccharide (LPS): dependence on LPS-binding protein and CD14, and inhibition by a recombinant fragment of bactericidal/permeability-increasing protein. Blood. 1994 May 1;83(9):2516–2525. [PubMed] [Google Scholar]
  13. Schumann R. R., Leong S. R., Flaggs G. W., Gray P. W., Wright S. D., Mathison J. C., Tobias P. S., Ulevitch R. J. Structure and function of lipopolysaccharide binding protein. Science. 1990 Sep 21;249(4975):1429–1431. doi: 10.1126/science.2402637. [DOI] [PubMed] [Google Scholar]
  14. Theofan G., Horwitz A. H., Williams R. E., Liu P. S., Chan I., Birr C., Carroll S. F., Mészáros K., Parent J. B., Kasler H. An amino-terminal fragment of human lipopolysaccharide-binding protein retains lipid A binding but not CD14-stimulatory activity. J Immunol. 1994 Apr 1;152(7):3623–3629. [PubMed] [Google Scholar]
  15. Tobias P. S., Mathison J., Mintz D., Lee J. D., Kravchenko V., Kato K., Pugin J., Ulevitch R. J. Participation of lipopolysaccharide-binding protein in lipopolysaccharide-dependent macrophage activation. Am J Respir Cell Mol Biol. 1992 Sep;7(3):239–245. doi: 10.1165/ajrcmb/7.3.239. [DOI] [PubMed] [Google Scholar]
  16. Tobias P. S., Soldau K., Ulevitch R. J. Identification of a lipid A binding site in the acute phase reactant lipopolysaccharide binding protein. J Biol Chem. 1989 Jun 25;264(18):10867–10871. [PubMed] [Google Scholar]
  17. Tobias P. S., Ulevitch R. J. Control of lipopolysaccharide-high density lipoprotein binding by acute phase protein(s). J Immunol. 1983 Oct;131(4):1913–1916. [PubMed] [Google Scholar]
  18. Ulevitch R. J., Mathison J. C., Schumann R. R., Tobias P. S. A new model of macrophage stimulation by bacterial lipopolysaccharide. J Trauma. 1990 Dec;30(12 Suppl):S189–S192. [PubMed] [Google Scholar]
  19. Ulevitch R. J. Recognition of bacterial endotoxins by receptor-dependent mechanisms. Adv Immunol. 1993;53:267–289. doi: 10.1016/s0065-2776(08)60502-7. [DOI] [PubMed] [Google Scholar]
  20. Vodovotz Y., Kwon N. S., Pospischil M., Manning J., Paik J., Nathan C. Inactivation of nitric oxide synthase after prolonged incubation of mouse macrophages with IFN-gamma and bacterial lipopolysaccharide. J Immunol. 1994 Apr 15;152(8):4110–4118. [PubMed] [Google Scholar]
  21. White M. L., Ma J. K., Birr C. A., Trown P. W., Carroll S. F. Measurement of bactericidal/permeability-increasing protein in human body fluids by sandwich ELISA. J Immunol Methods. 1994 Jan 3;167(1-2):227–235. doi: 10.1016/0022-1759(94)90091-4. [DOI] [PubMed] [Google Scholar]
  22. Wright S. D., Ramos R. A., Tobias P. S., Ulevitch R. J., Mathison J. C. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 1990 Sep 21;249(4975):1431–1433. doi: 10.1126/science.1698311. [DOI] [PubMed] [Google Scholar]
  23. Wurfel M. M., Kunitake S. T., Lichenstein H., Kane J. P., Wright S. D. Lipopolysaccharide (LPS)-binding protein is carried on lipoproteins and acts as a cofactor in the neutralization of LPS. J Exp Med. 1994 Sep 1;180(3):1025–1035. doi: 10.1084/jem.180.3.1025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yang Z., Khemlani L. S., Dean D. F., Carter C. D., Slauson D. O., Bochsler P. N. Serum components enhance bacterial lipopolysaccharide-induced tissue factor expression and tumor necrosis factor-alpha secretion by bovine alveolar macrophages in vitro. J Leukoc Biol. 1994 Apr;55(4):483–488. doi: 10.1002/jlb.55.4.483. [DOI] [PubMed] [Google Scholar]
  25. van Deventer S. J., Buller H. R., ten Cate J. W., Sturk A., Pauw W. Endotoxaemia: an early predictor of septicaemia in febrile patients. Lancet. 1988 Mar 19;1(8586):605–609. doi: 10.1016/s0140-6736(88)91412-2. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES