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Infection and Immunity logoLink to Infection and Immunity
. 1995 Feb;63(2):416–422. doi: 10.1128/iai.63.2.416-422.1995

Binding of type 1-piliated Escherichia coli to vaginal mucus.

M F Venegas 1, E L Navas 1, R A Gaffney 1, J L Duncan 1, B E Anderson 1, A J Schaeffer 1
PMCID: PMC173011  PMID: 7822005

Abstract

To better understand the interactions involved in bacterial adherence and the role of mucus in the pathogenesis of urinary tract infections, we developed a system to study the binding of a recombinant Escherichia coli strain, HB101/pWRS1-17, expressing type 1 pili, to vaginal mucus collected from 28 women. Bacteria bound to differing extents to all specimens examined, and preincubation of bacteria with mannose inhibited binding by 50 to 89%. Additionally, all mucus samples showed reactivity with anti-mannose antibody, and the levels of reactivity correlated with the levels of bacterial binding, suggesting that the mannose-terminal saccharides present on these glycoproteins are the receptors for the binding of type 1-piliated bacteria. Mucus specimens collected over periods of 5 days and 12 weeks exhibited significant variation in bacterial binding, indicating temporal differences in the ability of vaginal mucus to act as a receptor for type 1-piliated E. coli. The results show that vaginal mucus can bind bacteria and may thus influence the initial attachment and subsequent colonization of the vaginal and urinary tract epithelium by E. coli.

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Selected References

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  1. Babu J. P., Abraham S. N., Dabbous M. K., Beachey E. H. Interaction of a 60-kilodalton D-mannose-containing salivary glycoprotein with type 1 fimbriae of Escherichia coli. Infect Immun. 1986 Oct;54(1):104–108. doi: 10.1128/iai.54.1.104-108.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bloch C. A., Stocker B. A., Orndorff P. E. A key role for type 1 pili in enterobacterial communicability. Mol Microbiol. 1992 Mar;6(6):697–701. doi: 10.1111/j.1365-2958.1992.tb01518.x. [DOI] [PubMed] [Google Scholar]
  3. Duguid J. P., Clegg S., Wilson M. I. The fimbrial and non-fimbrial haemagglutinins of Escherichia coli. J Med Microbiol. 1979 May;12(2):213–227. doi: 10.1099/00222615-12-2-213. [DOI] [PubMed] [Google Scholar]
  4. Duncan J. L. Differential effect of Tamm-Horsfall protein on adherence of Escherichia coli to transitional epithelial cells. J Infect Dis. 1988 Dec;158(6):1379–1382. doi: 10.1093/infdis/158.6.1379. [DOI] [PubMed] [Google Scholar]
  5. Falk P., Roth K. A., Borén T., Westblom T. U., Gordon J. I., Normark S. An in vitro adherence assay reveals that Helicobacter pylori exhibits cell lineage-specific tropism in the human gastric epithelium. Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):2035–2039. doi: 10.1073/pnas.90.5.2035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fowler J. E., Jr, Stamey T. A. Studies of introital colonization in women with recurrent urinary infections. VII. The role of bacterial adherence. J Urol. 1977 Apr;117(4):472–476. doi: 10.1016/s0022-5347(17)58501-8. [DOI] [PubMed] [Google Scholar]
  7. Fujita K., Yamamoto T., Yokota T., Kitagawa R. In vitro adherence of type 1-fimbriated uropathogenic Escherichia coli to human ureteral mucosa. Infect Immun. 1989 Aug;57(8):2574–2579. doi: 10.1128/iai.57.8.2574-2579.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Guerina N. G., Kessler T. W., Guerina V. J., Neutra M. R., Clegg H. W., Langermann S., Scannapieco F. A., Goldmann D. A. The role of pili and capsule in the pathogenesis of neonatal infection with Escherichia coli K1. J Infect Dis. 1983 Sep;148(3):395–405. doi: 10.1093/infdis/148.3.395. [DOI] [PubMed] [Google Scholar]
  9. Guerina N. G., Woodson K., Hirshfeld D., Goldmann D. A. Heterologous protection against invasive Escherichia coli K1 disease in newborn rats by maternal immunization with purified mannose-sensitive pili. Infect Immun. 1989 May;57(5):1568–1572. doi: 10.1128/iai.57.5.1568-1572.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johnson J. R. Virulence factors in Escherichia coli urinary tract infection. Clin Microbiol Rev. 1991 Jan;4(1):80–128. doi: 10.1128/cmr.4.1.80. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kisielius P. V., Schwan W. R., Amundsen S. K., Duncan J. L., Schaeffer A. J. In vivo expression and variation of Escherichia coli type 1 and P pili in the urine of adults with acute urinary tract infections. Infect Immun. 1989 Jun;57(6):1656–1662. doi: 10.1128/iai.57.6.1656-1662.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Klemm P., Orskov I., Orskov F. F7 and type 1-like fimbriae from three Escherichia coli strains isolated from urinary tract infections: protein chemical and immunological aspects. Infect Immun. 1982 May;36(2):462–468. doi: 10.1128/iai.36.2.462-468.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Konijn A. M., Levy R., Link G., Hershko C. A rapid and sensitive ELISA for serum ferritin employing a fluorogenic substrate. J Immunol Methods. 1982 Nov 12;54(3):297–307. doi: 10.1016/0022-1759(82)90314-3. [DOI] [PubMed] [Google Scholar]
  14. Kuriyama S. M., Silverblatt F. J. Effect of Tamm-Horsfall urinary glycoprotein on phagocytosis and killing of type I-fimbriated Escherichia coli. Infect Immun. 1986 Jan;51(1):193–198. doi: 10.1128/iai.51.1.193-198.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lomberg H., Cedergren B., Leffler H., Nilsson B., Carlström A. S., Svanborg-Edén C. Influence of blood group on the availability of receptors for attachment of uropathogenic Escherichia coli. Infect Immun. 1986 Mar;51(3):919–926. doi: 10.1128/iai.51.3.919-926.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mantle M., Husar S. D. Adhesion of Yersinia enterocolitica to purified rabbit and human intestinal mucin. Infect Immun. 1993 Jun;61(6):2340–2346. doi: 10.1128/iai.61.6.2340-2346.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moldwin R. M., Shupp-Byrne D., Callahan H. J., Mulholland S. G. The presence of an antibacterial glycoprotein in a spectrum of transitional gel carcinomas. J Urol. 1992 Jul;148(1):154–157. doi: 10.1016/s0022-5347(17)36543-6. [DOI] [PubMed] [Google Scholar]
  18. Murray P. A., Prakobphol A., Lee T., Hoover C. I., Fisher S. J. Adherence of oral streptococci to salivary glycoproteins. Infect Immun. 1992 Jan;60(1):31–38. doi: 10.1128/iai.60.1.31-38.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Navas E. L., Venegas M. F., Duncan J. L., Anderson B. E., Chmiel J. S., Schaeffer A. J. Blood group antigen expression on vaginal and buccal epithelial cells and mucus in secretor and nonsecretor women. J Urol. 1993 Jun;149(6):1492–1498. doi: 10.1016/s0022-5347(17)36425-x. [DOI] [PubMed] [Google Scholar]
  20. O'Hanley P., Lark D., Falkow S., Schoolnik G. Molecular basis of Escherichia coli colonization of the upper urinary tract in BALB/c mice. Gal-Gal pili immunization prevents Escherichia coli pyelonephritis in the BALB/c mouse model of human pyelonephritis. J Clin Invest. 1985 Feb;75(2):347–360. doi: 10.1172/JCI111707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Old D. C. Inhibition of the interaction between fimbrial haemagglutinins and erythrocytes by D-mannose and other carbohydrates. J Gen Microbiol. 1972 Jun;71(1):149–157. doi: 10.1099/00221287-71-1-149. [DOI] [PubMed] [Google Scholar]
  22. Orskov I., Ferencz A., Orskov F. Tamm-Horsfall protein or uromucoid is the normal urinary slime that traps type 1 fimbriated Escherichia coli. Lancet. 1980 Apr 19;1(8173):887–887. doi: 10.1016/s0140-6736(80)91396-3. [DOI] [PubMed] [Google Scholar]
  23. Orskov I., Orskov F., Birch-Andersen A. Comparison of Escherichia coli fimbrial antigen F7 with type 1 fimbriae. Infect Immun. 1980 Feb;27(2):657–666. doi: 10.1128/iai.27.2.657-666.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ruggieri M. R., Balagani R. K., Rajter J. J., Hanno P. M. Characterization of bovine bladder mucin fractions that inhibit Escherichia coli adherence to the mucin deficient rabbit bladder. J Urol. 1992 Jul;148(1):173–178. doi: 10.1016/s0022-5347(17)36547-3. [DOI] [PubMed] [Google Scholar]
  25. Ruggieri M. R., Levin R. M., Hanno P. M., Witkowski B. A., Gill H. S., Steinhardt G. F. Defective antiadherence activity of bladder extracts from patients with recurrent urinary tract infection. J Urol. 1988 Jul;140(1):157–159. doi: 10.1016/s0022-5347(17)41517-5. [DOI] [PubMed] [Google Scholar]
  26. Sajjan S. U., Forstner J. F. Characteristics of binding of Escherichia coli serotype O157:H7 strain CL-49 to purified intestinal mucin. Infect Immun. 1990 Apr;58(4):860–867. doi: 10.1128/iai.58.4.860-867.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sajjan U. S., Corey M., Karmali M. A., Forstner J. F. Binding of Pseudomonas cepacia to normal human intestinal mucin and respiratory mucin from patients with cystic fibrosis. J Clin Invest. 1992 Feb;89(2):648–656. doi: 10.1172/JCI115631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sajjan U., Reisman J., Doig P., Irvin R. T., Forstner G., Forstner J. Binding of nonmucoid Pseudomonas aeruginosa to normal human intestinal mucin and respiratory mucin from patients with cystic fibrosis. J Clin Invest. 1992 Feb;89(2):657–665. doi: 10.1172/JCI115632. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sanford B. A., Thomas V. L., Ramsay M. A. Binding of staphylococci to mucus in vivo and in vitro. Infect Immun. 1989 Dec;57(12):3735–3742. doi: 10.1128/iai.57.12.3735-3742.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Schoolrik G. K. How Escherichia coli infects the urinary tract. N Engl J Med. 1989 Mar 23;320(12):804–805. doi: 10.1056/NEJM198903233201211. [DOI] [PubMed] [Google Scholar]
  31. Schroten H., Hanisch F. G., Plogmann R., Hacker J., Uhlenbruck G., Nobis-Bosch R., Wahn V. Inhibition of adhesion of S-fimbriated Escherichia coli to buccal epithelial cells by human milk fat globule membrane components: a novel aspect of the protective function of mucins in the nonimmunoglobulin fraction. Infect Immun. 1992 Jul;60(7):2893–2899. doi: 10.1128/iai.60.7.2893-2899.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Schwan W. R., Seifert H. S., Duncan J. L. Growth conditions mediate differential transcription of fim genes involved in phase variation of type 1 pili. J Bacteriol. 1992 Apr;174(7):2367–2375. doi: 10.1128/jb.174.7.2367-2375.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sheinfeld J., Schaeffer A. J., Cordon-Cardo C., Rogatko A., Fair W. R. Association of the Lewis blood-group phenotype with recurrent urinary tract infections in women. N Engl J Med. 1989 Mar 23;320(12):773–777. doi: 10.1056/NEJM198903233201205. [DOI] [PubMed] [Google Scholar]
  34. Simpson D. A., Ramphal R., Lory S. Genetic analysis of Pseudomonas aeruginosa adherence: distinct genetic loci control attachment to epithelial cells and mucins. Infect Immun. 1992 Sep;60(9):3771–3779. doi: 10.1128/iai.60.9.3771-3779.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Stamey T. A., Sexton C. C. The role of vaginal colonization with enterobacteriaceae in recurrent urinary infections. J Urol. 1975 Feb;113(2):214–217. doi: 10.1016/s0022-5347(17)59447-1. [DOI] [PubMed] [Google Scholar]
  36. Thomas V. L., Sanford B. A., Ramsay M. A. Calcium- and mucin-binding proteins of staphylococci. J Gen Microbiol. 1993 Mar;139(3):623–629. doi: 10.1099/00221287-139-3-623. [DOI] [PubMed] [Google Scholar]
  37. Venegas M., Liu L., Lovell L., Davis L. E., Anderson B., Wilbanks T., Hass M., Manderino G., Rittenhouse H. Purification and immunochemical characterization of ascitic fluid glycoproteins containing certain tumor-associated and blood group antigen markers. Glycoconj J. 1989;6(4):511–524. doi: 10.1007/BF01053774. [DOI] [PubMed] [Google Scholar]
  38. Vishwanath S., Ramphal R. Adherence of Pseudomonas aeruginosa to human tracheobronchial mucin. Infect Immun. 1984 Jul;45(1):197–202. doi: 10.1128/iai.45.1.197-202.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. WADDELL W. J. A simple ultraviolet spectrophotometric method for the determination of protein. J Lab Clin Med. 1956 Aug;48(2):311–314. [PubMed] [Google Scholar]

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