Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1995 Feb;63(2):696–699. doi: 10.1128/iai.63.2.696-699.1995

The gene encoding protein D (hpd) is highly conserved among Haemophilus influenzae type b and nontypeable strains.

X M Song 1, A Forsgren 1, H Janson 1
PMCID: PMC173053  PMID: 7822043

Abstract

The molecular conservation of a surface-exposed lipoprotein, protein D, of Haemophilus influenzae was studied by cloning and sequencing of the gene encoding protein D from three encapsulated type b strains and three nontypeable strains of H. influenzae. These nucleotide sequences were analyzed with previously reported sequences from one type b strain and one nontypeable strain. The nucleotide sequences and the deduced amino acid sequences for protein D were highly conserved. The deduced amino acid sequence (364 amino acids) of protein D from six strains differed only in two amino acids near the C-terminal end. The remaining two strains, one type b and one nontypeable, differed from the consensus sequence in 7 amino acids each. Protein D is 64 and 36% identical and 77 and 56% similar to the glycerophosphodiester phosphodiesterases (GlpQ) of Escherichia coli and Bacillus subtilis.

Full Text

The Full Text of this article is available as a PDF (207.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams W. G., Deaver K. A., Cochi S. L., Plikaytis B. D., Zell E. R., Broome C. V., Wenger J. D. Decline of childhood Haemophilus influenzae type b (Hib) disease in the Hib vaccine era. JAMA. 1993 Jan 13;269(2):221–226. [PubMed] [Google Scholar]
  2. Akkoyunlu M., Ruan M., Forsgren A. Distribution of protein D, an immunoglobulin D-binding protein, in Haemophilus strains. Infect Immun. 1991 Apr;59(4):1231–1238. doi: 10.1128/iai.59.4.1231-1238.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berk S. L., Holtsclaw S. A., Wiener S. L., Smith J. K. Nontypeable Haemophilus influenzae in the elderly. Arch Intern Med. 1982 Mar;142(3):537–539. [PubMed] [Google Scholar]
  4. Deich R. A., Anilionis A., Fulginiti J., Metcalf B. J., Quataert S., Quinn-Dey T., Zlotnick G. W., Green B. A. Antigenic conservation of the 15,000-dalton outer membrane lipoprotein PCP of Haemophilus influenzae and biologic activity of anti-PCP antisera. Infect Immun. 1990 Oct;58(10):3388–3393. doi: 10.1128/iai.58.10.3388-3393.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Green B. A., Farley J. E., Quinn-Dey T., Deich R. A., Zlotnick G. W. The e (P4) outer membrane protein of Haemophilus influenzae: biologic activity of anti-e serum and cloning and sequencing of the structural gene. Infect Immun. 1991 Sep;59(9):3191–3198. doi: 10.1128/iai.59.9.3191-3198.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Green B. A., Quinn-Dey T., Zlotnick G. W. Biologic activities of antibody to a peptidoglycan-associated lipoprotein of Haemophilus influenzae against multiple clinical isolates of H. influenzae type b. Infect Immun. 1987 Dec;55(12):2878–2883. doi: 10.1128/iai.55.12.2878-2883.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Janson H., Hedén L. O., Forsgren A. Protein D, the immunoglobulin D-binding protein of Haemophilus influenzae, is a lipoprotein. Infect Immun. 1992 Apr;60(4):1336–1342. doi: 10.1128/iai.60.4.1336-1342.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Janson H., Hedén L. O., Grubb A., Ruan M. R., Forsgren A. Protein D, an immunoglobulin D-binding protein of Haemophilus influenzae: cloning, nucleotide sequence, and expression in Escherichia coli. Infect Immun. 1991 Jan;59(1):119–125. doi: 10.1128/iai.59.1.119-125.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Janson H., Ruan M., Forsgren A. Limited diversity of the protein D gene (hpd) among encapsulated and nonencapsulated Haemophilus influenzae strains. Infect Immun. 1993 Nov;61(11):4546–4552. doi: 10.1128/iai.61.11.4546-4552.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kimura A., Gulig P. A., McCracken G. H., Jr, Loftus T. A., Hansen E. J. A minor high-molecular-weight outer membrane protein of Haemophilus influenzae type b is a protective antigen. Infect Immun. 1985 Jan;47(1):253–259. doi: 10.1128/iai.47.1.253-259.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kroll J. S., Moxon E. R. Capsulation in distantly related strains of Haemophilus influenzae type b: genetic drift and gene transfer at the capsulation locus. J Bacteriol. 1990 Mar;172(3):1374–1379. doi: 10.1128/jb.172.3.1374-1379.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Larson T. J., Ehrmann M., Boos W. Periplasmic glycerophosphodiester phosphodiesterase of Escherichia coli, a new enzyme of the glp regulon. J Biol Chem. 1983 May 10;258(9):5428–5432. [PubMed] [Google Scholar]
  14. Loeb M. R. Protection of infant rats from Haemophilus influenzae type b infection by antiserum to purified outer membrane protein a. Infect Immun. 1987 Nov;55(11):2612–2618. doi: 10.1128/iai.55.11.2612-2618.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Munson R. S., Jr, Granoff D. M. Purification and partial characterization of outer membrane proteins P5 and P6 from Haemophilus influenzae type b. Infect Immun. 1985 Sep;49(3):544–549. doi: 10.1128/iai.49.3.544-549.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Munson R. S., Jr, Sasaki K. Protein D, a putative immunoglobulin D-binding protein produced by Haemophilus influenzae, is glycerophosphodiester phosphodiesterase. J Bacteriol. 1993 Jul;175(14):4569–4571. doi: 10.1128/jb.175.14.4569-4571.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Munson R. S., Jr, Shenep J. L., Barenkamp S. J., Granoff D. M. Purification and comparison of outer membrane protein P2 from Haemophilus influenzae type b isolates. J Clin Invest. 1983 Aug;72(2):677–684. doi: 10.1172/JCI111017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Murphy T. F., Apicella M. A. Nontypable Haemophilus influenzae: a review of clinical aspects, surface antigens, and the human immune response to infection. Rev Infect Dis. 1987 Jan-Feb;9(1):1–15. doi: 10.1093/clinids/9.1.1. [DOI] [PubMed] [Google Scholar]
  19. Musser J. M., Kroll J. S., Moxon E. R., Selander R. K. Clonal population structure of encapsulated Haemophilus influenzae. Infect Immun. 1988 Aug;56(8):1837–1845. doi: 10.1128/iai.56.8.1837-1845.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nilsson R. P., Beijer L., Rutberg B. The glpT and glpQ genes of the glycerol regulon in Bacillus subtilis. Microbiology. 1994 Apr;140(Pt 4):723–730. doi: 10.1099/00221287-140-4-723. [DOI] [PubMed] [Google Scholar]
  21. Ruan M. R., Akkoyunlu M., Grubb A., Forsgren A. Protein D of Haemophilus influenzae. A novel bacterial surface protein with affinity for human IgD. J Immunol. 1990 Nov 15;145(10):3379–3384. [PubMed] [Google Scholar]
  22. Tommassen J., Eiglmeier K., Cole S. T., Overduin P., Larson T. J., Boos W. Characterization of two genes, glpQ and ugpQ, encoding glycerophosphoryl diester phosphodiesterases of Escherichia coli. Mol Gen Genet. 1991 Apr;226(1-2):321–327. doi: 10.1007/BF00273621. [DOI] [PubMed] [Google Scholar]
  23. Wu H. C., Tokunaga M. Biogenesis of lipoproteins in bacteria. Curr Top Microbiol Immunol. 1986;125:127–157. doi: 10.1007/978-3-642-71251-7_9. [DOI] [PubMed] [Google Scholar]
  24. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES