Abstract
The virulence properties of Escherichia coli O157 isolates were compared with those of Shiga-like-toxin-producing E. coli of non-O157 serogroups. The growth of all E. coli O157 isolates was stimulated by both heme and hemoglobin, and all produced enterohemolysin. The incidence of these properties was significantly lower in the non-O157 isolates. This may contribute to the greater virulence and higher incidence of human infection caused by E. coli O157.
Full Text
The Full Text of this article is available as a PDF (153.3 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barrett T. J., Kaper J. B., Jerse A. E., Wachsmuth I. K. Virulence factors in Shiga-like toxin-producing Escherichia coli isolated from humans and cattle. J Infect Dis. 1992 May;165(5):979–980. doi: 10.1093/infdis/165.5.979. [DOI] [PubMed] [Google Scholar]
- Beutin L., Montenegro M. A., Orskov I., Orskov F., Prada J., Zimmermann S., Stephan R. Close association of verotoxin (Shiga-like toxin) production with enterohemolysin production in strains of Escherichia coli. J Clin Microbiol. 1989 Nov;27(11):2559–2564. doi: 10.1128/jcm.27.11.2559-2564.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burnens A. P., Boss P., Orskov F., Orskov I., Schaad U. B., Müller F., Heinzle R., Nicolet J. Occurrence and phenotypic properties of verotoxin producing Escherichia coli in sporadic cases of gastroenteritis. Eur J Clin Microbiol Infect Dis. 1992 Jul;11(7):631–634. doi: 10.1007/BF01961673. [DOI] [PubMed] [Google Scholar]
- Donnenberg M. S., Tzipori S., McKee M. L., O'Brien A. D., Alroy J., Kaper J. B. The role of the eae gene of enterohemorrhagic Escherichia coli in intimate attachment in vitro and in a porcine model. J Clin Invest. 1993 Sep;92(3):1418–1424. doi: 10.1172/JCI116718. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Griffin P. M., Tauxe R. V. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol Rev. 1991;13:60–98. doi: 10.1093/oxfordjournals.epirev.a036079. [DOI] [PubMed] [Google Scholar]
- Jerse A. E., Yu J., Tall B. D., Kaper J. B. A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Natl Acad Sci U S A. 1990 Oct;87(20):7839–7843. doi: 10.1073/pnas.87.20.7839. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karmali M. A., Petric M., Lim C., Fleming P. C., Arbus G. S., Lior H. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli. J Infect Dis. 1985 May;151(5):775–782. doi: 10.1093/infdis/151.5.775. [DOI] [PubMed] [Google Scholar]
- Kleanthous H., Smith H. R., Scotland S. M., Gross R. J., Rowe B., Taylor C. M., Milford D. V. Haemolytic uraemic syndromes in the British Isles, 1985-8: association with verocytotoxin producing Escherichia coli. Part 2: Microbiological aspects. Arch Dis Child. 1990 Jul;65(7):722–727. doi: 10.1136/adc.65.7.722. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Law D. Adhesion and its role in the virulence of enteropathogenic Escherichia coli. Clin Microbiol Rev. 1994 Apr;7(2):152–173. doi: 10.1128/cmr.7.2.152. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Law D., Ganguli L. A., Donohue-Rolfe A., Acheson D. W. Detection by ELISA of low numbers of Shiga-like toxin-producing Escherichia coli in mixed cultures after growth in the presence of mitomycin C. J Med Microbiol. 1992 Mar;36(3):198–202. doi: 10.1099/00222615-36-3-198. [DOI] [PubMed] [Google Scholar]
- Lawlor K. M., Daskaleros P. A., Robinson R. E., Payne S. M. Virulence of iron transport mutants of Shigella flexneri and utilization of host iron compounds. Infect Immun. 1987 Mar;55(3):594–599. doi: 10.1128/iai.55.3.594-599.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Brien A. D., Holmes R. K. Shiga and Shiga-like toxins. Microbiol Rev. 1987 Jun;51(2):206–220. doi: 10.1128/mr.51.2.206-220.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pickett C. L., Auffenberg T., Pesci E. C., Sheen V. L., Jusuf S. S. Iron acquisition and hemolysin production by Campylobacter jejuni. Infect Immun. 1992 Sep;60(9):3872–3877. doi: 10.1128/iai.60.9.3872-3877.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riley L. W. The epidemiologic, clinical, and microbiologic features of hemorrhagic colitis. Annu Rev Microbiol. 1987;41:383–407. doi: 10.1146/annurev.mi.41.100187.002123. [DOI] [PubMed] [Google Scholar]
- Ritchie M., Partington S., Jessop J., Kelly M. T. Comparison of a direct fecal Shiga-like toxin assay and sorbitol-MacConkey agar culture for laboratory diagnosis of enterohemorrhagic Escherichia coli infection. J Clin Microbiol. 1992 Feb;30(2):461–464. doi: 10.1128/jcm.30.2.461-464.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scotland S. M., Willshaw G. A., Smith H. R., Rowe B. Properties of strains of Escherichia coli O26:H11 in relation to their enteropathogenic or enterohemorrhagic classification. J Infect Dis. 1990 Nov;162(5):1069–1074. doi: 10.1093/infdis/162.5.1069. [DOI] [PubMed] [Google Scholar]
- Sjogren R., Neill R., Rachmilewitz D., Fritz D., Newland J., Sharpnack D., Colleton C., Fondacaro J., Gemski P., Boedeker E. Role of Shiga-like toxin I in bacterial enteritis: comparison between isogenic Escherichia coli strains induced in rabbits. Gastroenterology. 1994 Feb;106(2):306–317. doi: 10.1016/0016-5085(94)90587-8. [DOI] [PubMed] [Google Scholar]
- Smith H. R., Cheasty T., Roberts D., Thomas A., Rowe B. Examination of retail chickens and sausages in Britain for vero cytotoxin-producing Escherichia coli. Appl Environ Microbiol. 1991 Jul;57(7):2091–2093. doi: 10.1128/aem.57.7.2091-2093.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tesh V. L., Burris J. A., Owens J. W., Gordon V. M., Wadolkowski E. A., O'Brien A. D., Samuel J. E. Comparison of the relative toxicities of Shiga-like toxins type I and type II for mice. Infect Immun. 1993 Aug;61(8):3392–3402. doi: 10.1128/iai.61.8.3392-3402.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tesh V. L., O'Brien A. D. Adherence and colonization mechanisms of enteropathogenic and enterohemorrhagic Escherichia coli. Microb Pathog. 1992 Apr;12(4):245–254. doi: 10.1016/0882-4010(92)90043-n. [DOI] [PubMed] [Google Scholar]
- Tokhi A. M., Peiris J. S., Scotland S. M., Willshaw G. A., Smith H. R., Cheasty T. A longitudinal study of Vero cytotoxin producing Escherichia coli in cattle calves in Sri Lanka. Epidemiol Infect. 1993 Apr;110(2):197–208. doi: 10.1017/s0950268800068114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wadolkowski E. A., Sung L. M., Burris J. A., Samuel J. E., O'Brien A. D. Acute renal tubular necrosis and death of mice orally infected with Escherichia coli strains that produce Shiga-like toxin type II. Infect Immun. 1990 Dec;58(12):3959–3965. doi: 10.1128/iai.58.12.3959-3965.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whittam T. S., Wachsmuth I. K., Wilson R. A. Genetic evidence of clonal descent of Escherichia coli O157:H7 associated with hemorrhagic colitis and hemolytic uremic syndrome. J Infect Dis. 1988 Jun;157(6):1124–1133. doi: 10.1093/infdis/157.6.1124. [DOI] [PubMed] [Google Scholar]
- Willshaw G. A., Scotland S. M., Smith H. R., Rowe B. Properties of Vero cytotoxin-producing Escherichia coli of human origin of O serogroups other than O157. J Infect Dis. 1992 Oct;166(4):797–802. doi: 10.1093/infdis/166.4.797. [DOI] [PubMed] [Google Scholar]