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. 1995 Apr;63(4):1340–1348. doi: 10.1128/iai.63.4.1340-1348.1995

Pasteurella haemolytica serotype 2 contains the gene for a noncapsular serotype 1-specific antigen.

C T Gonzalez 1, S K Maheswaran 1, M P Murtaugh 1
PMCID: PMC173156  PMID: 7890392

Abstract

An ssa1-homologous genomic fragment cloned from Pasteurella haemolytica serotype 2 (ST2) enabled transformation of Escherichia coli DH5 alpha to a serotype 1 (ST1) phenotype through expression of the ST1-specific antigen (Ssa1). The Ssa1 protein expressed by ssa1-transformed E. coli was susceptible to heat and protease treatment and was distinct from P. haemolytica ST1-specific capsular polysaccharide. Electrophoretic analysis of in vitro-translated proteins, as well as the predicted amino acid sequence, demonstrated that Ssa1 proteins encoded from either ST1- or ST2-derived ssa1 genes were essentially identical. A comparison of the nucleotide sequences of ssa1 genes derived from P. haemolytica ST1 and ST2 revealed greater than 99% homology. Amino acid sequence homology of the predicted products of ST1- and ST2-derived ssa1 genes was greater than 98%. Northern (RNA) blot studies revealed that the presence of an increased level of ssa1 transcript in P. haemolytica ST1 grown as surface-adherent cultures on solid medium was correlated with a serologically detectable Ssa1 protein. Expression of the ssa1 transcript in ST1 was similarly upregulated by a high iron concentration in the growth medium.

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Selected References

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  1. Allan E. M., Gibbs H. A., Wiseman A., Selman I. E. Sequential lesions of experimental bovine pneumonic pasteurellosis. Vet Rec. 1985 Oct 26;117(17):438–442. doi: 10.1136/vr.117.17.438. [DOI] [PubMed] [Google Scholar]
  2. BIBERSTEIN E. L., GILLS M., KNIGHT H. Serological types of Pasteurella hemolytica. Cornell Vet. 1960 Jul;50:283–300. [PubMed] [Google Scholar]
  3. Beyer C. F. A 'dot-immunobinding assay' on nitrocellulose membrane for the determination of the immunoglobulin class of mouse monoclonal antibodies. J Immunol Methods. 1984 Feb 24;67(1):79–87. doi: 10.1016/0022-1759(84)90087-5. [DOI] [PubMed] [Google Scholar]
  4. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  5. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  6. Eisenberg D., Schwarz E., Komaromy M., Wall R. Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J Mol Biol. 1984 Oct 15;179(1):125–142. doi: 10.1016/0022-2836(84)90309-7. [DOI] [PubMed] [Google Scholar]
  7. Eisenberg D., Weiss R. M., Terwilliger T. C. The hydrophobic moment detects periodicity in protein hydrophobicity. Proc Natl Acad Sci U S A. 1984 Jan;81(1):140–144. doi: 10.1073/pnas.81.1.140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fox M. L., Thomson R. G., Magwood S. E. Pasteurella haemolytica of cattle: serotype, production of beta-galactosidase and antibacterial sensitivity. Can J Comp Med. 1971 Oct;35(4):313–317. [PMC free article] [PubMed] [Google Scholar]
  9. Frank G. H., Briggs R. E., Gillette K. G. Pasteurella haemolytica serotype 1 colonization of the nasal passages of virus-infected calves. Am J Vet Res. 1987 Dec;48(12):1674–1677. [PubMed] [Google Scholar]
  10. Frank G. H. Pasteurella haemolytica and respiratory disease in cattle. Proc Annu Meet U S Anim Health Assoc. 1979;(83):153–160. [PubMed] [Google Scholar]
  11. Frank G. H. Serotype-specific resistance to nasal colonization by Pasteurella haemolytica in cattle. Am J Vet Res. 1985 Nov;46(11):2245–2248. [PubMed] [Google Scholar]
  12. Frank G. H., Smith P. C. Prevalence of Pasteurella haemolytica in transported calves. Am J Vet Res. 1983 Jun;44(6):981–985. [PubMed] [Google Scholar]
  13. Frank G. H., Wessman G. E. Rapid plate agglutination procedure for serotyping Pasteurella haemolytica. J Clin Microbiol. 1978 Feb;7(2):142–145. doi: 10.1128/jcm.7.2.142-145.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Garnier J., Osguthorpe D. J., Robson B. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J Mol Biol. 1978 Mar 25;120(1):97–120. doi: 10.1016/0022-2836(78)90297-8. [DOI] [PubMed] [Google Scholar]
  15. Gentry M. J., Confer A. W., Weinberg E. D., Homer J. T. Cytotoxin (leukotoxin) production by Pasteurella haemolytica: requirement for an iron-containing compound. Am J Vet Res. 1986 Sep;47(9):1919–1923. [PubMed] [Google Scholar]
  16. Gonzalez-Rayos C., Lo R. Y., Shewen P. E., Beveridge T. J. Cloning of a serotype-specific antigen from Pasteurella haemolytica A1. Infect Immun. 1986 Sep;53(3):505–510. doi: 10.1128/iai.53.3.505-510.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Gonzalez C., Murtaugh M. P., Maheswaran S. K. Genomic distribution of a serotype 1-specific antigen-coding DNA fragment of Pasteurella haemolytica. Zentralbl Veterinarmed B. 1991 Oct;38(8):599–609. doi: 10.1111/j.1439-0450.1991.tb00917.x. [DOI] [PubMed] [Google Scholar]
  18. Grey C. L., Thomson R. G. Pasteurella haemolytica in the tracheal air of calves. Can J Comp Med. 1971 Apr;35(2):121–128. [PMC free article] [PubMed] [Google Scholar]
  19. Highlander S. K., Engler M. J., Weinstock G. M. Secretion and expression of the Pasteurella haemolytica Leukotoxin. J Bacteriol. 1990 May;172(5):2343–2350. doi: 10.1128/jb.172.5.2343-2350.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Highlander S. K., Wickersham E. A., Garza O., Weinstock G. M. Expression of the Pasteurella haemolytica leukotoxin is inhibited by a locus that encodes an ATP-binding cassette homolog. Infect Immun. 1993 Sep;61(9):3942–3951. doi: 10.1128/iai.61.9.3942-3951.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  22. Lo R. Y., Strathdee C. A., Shewen P. E., Cooney B. J. Molecular studies of Ssa1, a serotype-specific antigen of Pasteurella haemolytica A1. Infect Immun. 1991 Oct;59(10):3398–3406. doi: 10.1128/iai.59.10.3398-3406.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Penaredondo M. V., Maheswaran S. K., Whiteley L. O., Ames T. R., Leininger J. R. Production and partial characterization of monoclonal antibodies to Pasteurella haemolytica A1 capsular polysaccharide and lipopolysaccharide. Am J Vet Res. 1988 Nov;49(11):1962–1968. [PubMed] [Google Scholar]
  24. Rehmtulla A. J., Thomson R. G. A review of the lesions in shipping fever of cattle. Can Vet J. 1981 Jan;22(1):1–8. [PMC free article] [PubMed] [Google Scholar]
  25. Strathdee C. A., Lo R. Y. Regulation of expression of the Pasteurella haemolytica leukotoxin determinant. J Bacteriol. 1989 Nov;171(11):5955–5962. doi: 10.1128/jb.171.11.5955-5962.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wessman G. E., Hilker G. Characterization of Pasteurella hemolytica isolated from the respiratory tract of cattle. Can J Comp Med. 1968 Jul;32(3):498–504. [PMC free article] [PubMed] [Google Scholar]
  27. Yates W. D. A review of infectious bovine rhinotracheitis, shipping fever pneumonia and viral-bacterial synergism in respiratory disease of cattle. Can J Comp Med. 1982 Jul;46(3):225–263. [PMC free article] [PubMed] [Google Scholar]

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