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. 1995 Oct;63(10):4016–4023. doi: 10.1128/iai.63.10.4016-4023.1995

Association of lysogenic bacteriophage MAV1 with virulence of Mycoplasma arthritidis.

L L Voelker 1, K E Weaver 1, L J Ehle 1, L R Washburn 1
PMCID: PMC173564  PMID: 7558313

Abstract

Mycoplasma arthritidis causes a severe polyarthritis under natural conditions in rats and under experimental conditions in both rats and mice. Although the disease itself has been extensively studied, M. arthritidis virulence factors remain uncharacterized. Comparison of relative arthritogenicity of 20 strains of M. arthritidis revealed that the strains tended to fall into two groups, a highly arthritogenic group, inducing maximum arthritis scores of > or = 11 in rats, and a low-virulence group, inducing maximum scores of < 6. Chromosomal DNA from the more highly arthritogenic strains possessed sequences that hybridized by Southern analysis with a probe prepared from lysogenic M. arthritidis bacteriophage MAV1, while DNA from low-virulence strains did not. One of the low-virulence strains, 158, was experimentally lysogenized with MAV1. Lysogenized 158 showed a significant increase in arthritogenicity over nonlysogenized 158. These data suggest that MAV1 carries a factor that is important in pathogenesis of M. arthritidis-induced arthritis of rats.

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Selected References

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  1. Bergemann A. D., Finch L. R. Isolation and restriction endonuclease analysis of a mycoplasma plasmid. Plasmid. 1988 Jan;19(1):68–70. doi: 10.1016/0147-619x(88)90064-9. [DOI] [PubMed] [Google Scholar]
  2. Bhugra B., Dybvig K. Identification and characterization of IS1138, a transposable element from Mycoplasma pulmonis that belongs to the IS3 family. Mol Microbiol. 1993 Feb;7(4):577–584. doi: 10.1111/j.1365-2958.1993.tb01148.x. [DOI] [PubMed] [Google Scholar]
  3. Cole B. C., Ahmed E., Araneo B. A., Shelby J., Kamerath C., Wei S., McCall S., Atkin C. L. Immunomodulation in vivo by the Mycoplasma arthritidis superantigen, MAM. Clin Infect Dis. 1993 Aug;17 (Suppl 1):S163–S169. doi: 10.1093/clinids/17.supplement_1.s163. [DOI] [PubMed] [Google Scholar]
  4. Cole B. C., Atkin C. L. The Mycoplasma arthritidis T-cell mitogen, MAM: a model superantigen. Immunol Today. 1991 Aug;12(8):271–276. doi: 10.1016/0167-5699(91)90125-D. [DOI] [PubMed] [Google Scholar]
  5. Cole B. C., Cahill J. F., Wiley B. B., Ward J. R. Immunological responses of the rat to Mycoplasma arthritidis. J Bacteriol. 1969 Jun;98(3):930–937. doi: 10.1128/jb.98.3.930-937.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cole B. C., Miller M. L., Ward J. R. A comparative study on the virulence of Mycoplasma arthritidis and "Mycoplasma hominis, type 2" strains in rats. Proc Soc Exp Biol Med. 1967 Jan;124(1):103–107. doi: 10.3181/00379727-124-31676. [DOI] [PubMed] [Google Scholar]
  7. Cole B. C., Ward J. R., Golightly-Rowland L. [Factors influencing the susceptibility of mice to Mycoplasma arthritidis]. Infect Immun. 1973 Feb;7(2):218–225. doi: 10.1128/iai.7.2.218-225.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cole B. C., Ward J. R. Interaction of Mycoplasma arthritidis and other mycoplasmas with murine peritoneal macrophages. Infect Immun. 1973 May;7(5):691–699. doi: 10.1128/iai.7.5.691-699.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cole B. C., Ward J. R., Jones R. S., Cahill J. F. Chronic proliferative arthritis of mice induced by Mycoplasma arthritidis. I. Induction of disease and histopathological characteristics. Infect Immun. 1971 Oct;4(4):344–355. doi: 10.1128/iai.4.4.344-355.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Cox N. R., Davidson M. K., Davis J. K., Lindsey J. R., Cassell G. H. Natural mycoplasmal infections in isolator-maintained LEW/Tru rats. Lab Anim Sci. 1988 Aug;38(4):381–388. [PubMed] [Google Scholar]
  11. Del Giudice R. A., Robillard N. F., Carski T. R. Immunofluorescence identification of Mycoplasma on agar by use of incident illumination. J Bacteriol. 1967 Apr;93(4):1205–1209. doi: 10.1128/jb.93.4.1205-1209.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Dybvig K., Khaled M. Isolation of a second cryptic plasmid from Mycoplasma mycoides subsp. mycoides. Plasmid. 1990 Sep;24(2):153–155. doi: 10.1016/0147-619x(90)90018-8. [DOI] [PubMed] [Google Scholar]
  13. Dybvig K., Liss A., Alderete J., Cole R. M., Cassell G. H. Isolation of a virus from Mycoplasma pulmonis. Isr J Med Sci. 1987 May;23(5):418–422. [PubMed] [Google Scholar]
  14. Ferrell R. V., Heidari M. B., Wise K. S., McIntosh M. A. A Mycoplasma genetic element resembling prokaryotic insertion sequences. Mol Microbiol. 1989 Jul;3(7):957–967. doi: 10.1111/j.1365-2958.1989.tb00245.x. [DOI] [PubMed] [Google Scholar]
  15. Golightly-Rowland L., Cole B. C., Ward J. R., Wiley B. B. Effect of Animal Passage on Arthritogenic and Biological Properties of Mycoplasma arthritidis. Infect Immun. 1970 Jun;1(6):538–545. doi: 10.1128/iai.1.6.538-545.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Gourlay R. N., Wyld S. G., Poulton M. E. Some characteristics of mycoplasma virus Hr 1, isolated from and infecting Mycoplasma hyorhinis. Brief report. Arch Virol. 1983;77(1):81–85. doi: 10.1007/BF01314867. [DOI] [PubMed] [Google Scholar]
  17. Hannan P. C., Hughes B. O. Reproducible polyarthritis in rats caused by Mycoplasma arthritidis. Ann Rheum Dis. 1971 May;30(3):316–321. doi: 10.1136/ard.30.3.316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hill A., Dagnall G. J. Experimental polyarthritis in rats produced by Mycoplasma arthritidis. J Comp Pathol. 1975 Jan;85(1):45–52. doi: 10.1016/0021-9975(75)90083-3. [DOI] [PubMed] [Google Scholar]
  19. Hu W. S., Wang R. Y., Liou R. S., Shih J. W., Lo S. C. Identification of an insertion-sequence-like genetic element in the newly recognized human pathogen Mycoplasma incognitus. Gene. 1990 Sep 1;93(1):67–72. doi: 10.1016/0378-1119(90)90137-g. [DOI] [PubMed] [Google Scholar]
  20. Iandolo J. J. Genetic analysis of extracellular toxins of Staphylococcus aureus. Annu Rev Microbiol. 1989;43:375–402. doi: 10.1146/annurev.mi.43.100189.002111. [DOI] [PubMed] [Google Scholar]
  21. LEMCKE R. M. THE SEROLOGICAL DIFFERENTIATION OF MYCOPLASMA STRAINS (PLEURO-PNEUMONIA-LIKE ORGANISMS) FROM VARIOUS SOURCES. J Hyg (Lond) 1964 Jun;62:199–219. doi: 10.1017/s0022172400039930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. LIEBERMAN P. R., SMITH P. F., MORTON H. E. The susceptibility of pleuropneumonia-like organisms to the in vitro action of antibiotics: aureomycin, chloramphenicol, dihydrostreptomycin, streptomycin, and sodium penicillin G. J Urol. 1950 Jul;64(1):167–173. doi: 10.1016/S0022-5347(17)68616-6. [DOI] [PubMed] [Google Scholar]
  23. Laber G., Walzl H., Schütze E. Klinische und histopathologische Befunde bei der Mykoplasma-Polyarthritis der Ratte. I. Der Ablauf der Infektion in den ersten 8 Tagen. Zentralbl Bakteriol Orig A. 1975;230(3):385–397. [PubMed] [Google Scholar]
  24. NICOL C. S., EDWARD D. G. Role of organisms of the pleuropneumonia group in human genital infections. Br J Vener Dis. 1953 Sep;29(3):141–150. doi: 10.1136/sti.29.3.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. TULLY J. G. BIOCHEMICAL, MORPHOLOGICAL, AND SEROLOGICAL CHARACTERIZATION OF MYCOPLASMA OF MURINE ORIGIN. J Infect Dis. 1965 Apr;115:171–185. doi: 10.1093/infdis/115.2.171. [DOI] [PubMed] [Google Scholar]
  26. Washburn L. R., Cole B. C., Gelman M. I., Ward J. R. Chronic arthritis of rabbits induced by mycoplasmas. I. Clinical microbiologic, and histologic features. Arthritis Rheum. 1980 Jul;23(7):825–836. doi: 10.1002/art.1780230709. [DOI] [PubMed] [Google Scholar]
  27. Washburn L. R., Hirsch S. Comparison of four Mycoplasma arthritidis strains by enzyme immunoassay, metabolism inhibition, one- and two-dimensional electrophoresis, and immunoblotting. J Clin Microbiol. 1990 Sep;28(9):1974–1981. doi: 10.1128/jcm.28.9.1974-1981.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Washburn L. R., Hirsch S., McKenzie M., Voelker L. L. Vaccination of Lewis rats against Mycoplasma arthritidis-induced arthritis. Am J Vet Res. 1992 Jan;53(1):52–58. [PubMed] [Google Scholar]
  29. Washburn L. R., Hirsch S., Voelker L. L. Mechanisms of attachment of Mycoplasma arthritidis to host cells in vitro. Infect Immun. 1993 Jun;61(6):2670–2680. doi: 10.1128/iai.61.6.2670-2680.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Washburn L. R., Ramsay J. R. Experimental induction of arthritis in LEW rats and antibody response to four Mycoplasma arthritidis strains. Vet Microbiol. 1989 Nov;21(1):41–55. doi: 10.1016/0378-1135(89)90017-5. [DOI] [PubMed] [Google Scholar]
  31. Washburn L. R., Voelker L. L., Ehle L. J., Hirsch S., Dutenhofer C., Olson K., Beck B. Comparison of Mycoplasma arthritidis strains by enzyme-linked immunosorbent assay, immunoblotting, and DNA restriction analysis. J Clin Microbiol. 1995 Sep;33(9):2271–2279. doi: 10.1128/jcm.33.9.2271-2279.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]

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