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. 1995 Dec;63(12):4795–4801. doi: 10.1128/iai.63.12.4795-4801.1995

Variation in antigenicity and infectivity of derivatives of Borrelia burgdorferi, strain B31, maintained in the natural, zoonotic cycle compared with maintenance in culture.

W T Golde 1, M C Dolan 1
PMCID: PMC173687  PMID: 7591138

Abstract

The original isolate of Borrelia burgdorferi, strain B31, can be maintained in vitro indefinitely. A number of studies have demonstrated that there are recognizable changes in the genetic composition of the spirochete after more than 60 passages. We have maintained B31 in the natural zoonotic cycle of transmission of infection between laboratory mice and laboratory-reared Ixodes ticks. To determine whether similar changes occur in the natural transmission cycle, we reisolated strain B31 from mouse skin at the fifth zoonotic cycle. This reisolated derivative had the same infectivity as the parent B31 strain, had lost the 8-kb supercoiled plasmid present in B31, and induced a gross serum antibody response indistinguishable from the B31 immune response. Analysis of antigen expression with monoclonal antibodies generated against B31, however, showed differential expression of a subset of antigens between B31 and the isolated derivative.

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Selected References

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  1. Baranton G., Postic D., Saint Girons I., Boerlin P., Piffaretti J. C., Assous M., Grimont P. A. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. Int J Syst Bacteriol. 1992 Jul;42(3):378–383. doi: 10.1099/00207713-42-3-378. [DOI] [PubMed] [Google Scholar]
  2. Barthold S. W. Antigenic stability of Borrelia burgdorferi during chronic infections of immunocompetent mice. Infect Immun. 1993 Dec;61(12):4955–4961. doi: 10.1128/iai.61.12.4955-4961.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Becker Y. Dengue fever virus and Japanese encephalitis virus synthetic peptides, with motifs to fit HLA class I haplotypes prevalent in human populations in endemic regions, can be used for application to skin Langerhans cells to prime antiviral CD8+ cytotoxic T cells (CTLs)--a novel approach to the protection of humans. Virus Genes. 1994 Sep;9(1):33–45. doi: 10.1007/BF01703433. [DOI] [PubMed] [Google Scholar]
  4. Belfaiza J., Postic D., Bellenger E., Baranton G., Girons I. S. Genomic fingerprinting of Borrelia burgdorferi sensu lato by pulsed-field gel electrophoresis. J Clin Microbiol. 1993 Nov;31(11):2873–2877. doi: 10.1128/jcm.31.11.2873-2877.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dunn J. J., Buchstein S. R., Butler L. L., Fisenne S., Polin D. S., Lade B. N., Luft B. J. Complete nucleotide sequence of a circular plasmid from the Lyme disease spirochete, Borrelia burgdorferi. J Bacteriol. 1994 May;176(9):2706–2717. doi: 10.1128/jb.176.9.2706-2717.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Golde W. T., Burkot T. R., Sviat S., Keen M. G., Mayer L. W., Johnson B. J., Piesman J. The major histocompatibility complex-restricted response of recombinant inbred strains of mice to natural tick transmission of Borrelia burgdorferi. J Exp Med. 1993 Jan 1;177(1):9–17. doi: 10.1084/jem.177.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Golde W. T., Kappel K. J., Dequesne G., Feron C., Plainchamp D., Capiau C., Lobet Y. Tick transmission of Borrelia burgdorferi to inbred strains of mice induces an antibody response to P39 but not to outer surface protein A. Infect Immun. 1994 Jun;62(6):2625–2627. doi: 10.1128/iai.62.6.2625-2627.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hinnebusch J., Barbour A. G. Linear plasmids of Borrelia burgdorferi have a telomeric structure and sequence similar to those of a eukaryotic virus. J Bacteriol. 1991 Nov;173(22):7233–7239. doi: 10.1128/jb.173.22.7233-7239.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hinnebusch J., Barbour A. G. Linear- and circular-plasmid copy numbers in Borrelia burgdorferi. J Bacteriol. 1992 Aug;174(16):5251–5257. doi: 10.1128/jb.174.16.5251-5257.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Norris S. J., Carter C. J., Howell J. K., Barbour A. G. Low-passage-associated proteins of Borrelia burgdorferi B31: characterization and molecular cloning of OspD, a surface-exposed, plasmid-encoded lipoprotein. Infect Immun. 1992 Nov;60(11):4662–4672. doi: 10.1128/iai.60.11.4662-4672.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pachner A. R., Delaney E., Ricalton N. S. Murine Lyme borreliosis: route of inoculation determines immune response and infectivity. Reg Immunol. 1992 Nov-Dec;4(6):345–351. [PubMed] [Google Scholar]
  12. Persing D. H., Mathiesen D., Podzorski D., Barthold S. W. Genetic stability of Borrelia burgdorferi recovered from chronically infected immunocompetent mice. Infect Immun. 1994 Aug;62(8):3521–3527. doi: 10.1128/iai.62.8.3521-3527.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Piesman J., Sinsky R. J. Ability to Ixodes scapularis, Dermacentor variabilis, and Amblyomma americanum (Acari: Ixodidae) to acquire, maintain, and transmit Lyme disease spirochetes (Borrelia burgdorferi). J Med Entomol. 1988 Sep;25(5):336–339. doi: 10.1093/jmedent/25.5.336. [DOI] [PubMed] [Google Scholar]
  14. Piesman J. Standard system for infecting ticks (Acari: Ixodidae) with the Lyme disease spirochete, Borrelia burgdorferi. J Med Entomol. 1993 Jan;30(1):199–203. doi: 10.1093/jmedent/30.1.199. [DOI] [PubMed] [Google Scholar]
  15. Schaible U. E., Gern L., Wallich R., Kramer M. D., Prester M., Simon M. M. Distinct patterns of protective antibodies are generated against Borrelia burgdorferi in mice experimentally inoculated with high and low doses of antigen. Immunol Lett. 1993 May;36(2):219–226. doi: 10.1016/0165-2478(93)90056-8. [DOI] [PubMed] [Google Scholar]
  16. Schwan T. G., Burgdorfer W., Garon C. F. Changes in infectivity and plasmid profile of the Lyme disease spirochete, Borrelia burgdorferi, as a result of in vitro cultivation. Infect Immun. 1988 Aug;56(8):1831–1836. doi: 10.1128/iai.56.8.1831-1836.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schwan T. G., Karstens R. H., Schrumpf M. E., Simpson W. J. Changes in antigenic reactivity of Borrelia burgdorferi, the Lyme disease spirochete, during persistent infection in mice. Can J Microbiol. 1991 Jun;37(6):450–454. doi: 10.1139/m91-074. [DOI] [PubMed] [Google Scholar]
  18. Simpson W. J., Garon C. F., Schwan T. G. Analysis of supercoiled circular plasmids in infectious and non-infectious Borrelia burgdorferi. Microb Pathog. 1990 Feb;8(2):109–118. doi: 10.1016/0882-4010(90)90075-2. [DOI] [PubMed] [Google Scholar]
  19. Sinsky R. J., Piesman J. Ear punch biopsy method for detection and isolation of Borrelia burgdorferi from rodents. J Clin Microbiol. 1989 Aug;27(8):1723–1727. doi: 10.1128/jcm.27.8.1723-1727.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. de Souza M. S., Smith A. L., Beck D. S., Terwilliger G. A., Fikrig E., Barthold S. W. Long-term study of cell-mediated responses to Borrelia burgdorferi in the laboratory mouse. Infect Immun. 1993 May;61(5):1814–1822. doi: 10.1128/iai.61.5.1814-1822.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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