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. 1995 Dec;63(12):4959–4963. doi: 10.1128/iai.63.12.4959-4963.1995

Adherence to and invasion of tissue culture cells by Vibrio hollisae.

M D Miliotis 1, B D Tall 1, R T Gray 1
PMCID: PMC173716  PMID: 7591167

Abstract

The adherence to and invasion of cultured epithelial cells by Vibrio hollisae were examined by quantitative studies and by light, fluorescent, and electron microscopy. Condensed actin was observed around clustered adherent and intracellular bacteria. Bacteria multiplied intracellularly. Inhibitor studies indicated that internalization occurred by an integrated pleiotropic process involving eukaryotic and prokaryotic protein syntheses, microfilaments, microtubules, and receptor-mediated endocytosis.

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Selected References

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  1. Clerc P. L., Sansonetti P. J. Evidence for clathrin mobilization during directed phagocytosis of Shigella flexneri by HEp2 cells. Microb Pathog. 1989 Nov;7(5):329–336. doi: 10.1016/0882-4010(89)90036-3. [DOI] [PubMed] [Google Scholar]
  2. Detilleux P. G., Deyoe B. L., Cheville N. F. Effect of endocytic and metabolic inhibitors on the internalization and intracellular growth of Brucella abortus in Vero cells. Am J Vet Res. 1991 Oct;52(10):1658–1664. [PubMed] [Google Scholar]
  3. Donnenberg M. S., Donohue-Rolfe A., Keusch G. T. A comparison of HEp-2 cell invasion by enteropathogenic and enteroinvasive Escherichia coli. FEMS Microbiol Lett. 1990 May;57(1-2):83–86. doi: 10.1016/0378-1097(90)90417-o. [DOI] [PubMed] [Google Scholar]
  4. Elsinghorst E. A., Baron L. S., Kopecko D. J. Penetration of human intestinal epithelial cells by Salmonella: molecular cloning and expression of Salmonella typhi invasion determinants in Escherichia coli. Proc Natl Acad Sci U S A. 1989 Jul;86(13):5173–5177. doi: 10.1073/pnas.86.13.5173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Evans D. G., Evans D. J., Jr, Graham D. Y. Adherence and internalization of Helicobacter pylori by HEp-2 cells. Gastroenterology. 1992 May;102(5):1557–1567. doi: 10.1016/0016-5085(92)91714-f. [DOI] [PubMed] [Google Scholar]
  6. Evans D. G., Evans D. J., Jr, Tjoa W. Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor. Infect Immun. 1977 Nov;18(2):330–337. doi: 10.1128/iai.18.2.330-337.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gander R. M., LaRocco M. T. Detection of piluslike structures on clinical and environmental isolates of Vibrio vulnificus. J Clin Microbiol. 1989 May;27(5):1015–1021. doi: 10.1128/jcm.27.5.1015-1021.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hale T. L., Morris R. E., Bonventre P. F. Shigella infection of henle intestinal epithelial cells: role of the host cell. Infect Immun. 1979 Jun;24(3):887–894. doi: 10.1128/iai.24.3.887-894.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hodinka R. L., Wyrick P. B. Ultrastructural study of mode of entry of Chlamydia psittaci into L-929 cells. Infect Immun. 1986 Dec;54(3):855–863. doi: 10.1128/iai.54.3.855-863.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Knutton S., Baldwin T., Williams P. H., McNeish A. S. Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli. Infect Immun. 1989 Apr;57(4):1290–1298. doi: 10.1128/iai.57.4.1290-1298.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kothary M. H., Richardson S. H. Fluid accumulation in infant mice caused by Vibrio hollisae and its extracellular enterotoxin. Infect Immun. 1987 Mar;55(3):626–630. doi: 10.1128/iai.55.3.626-630.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Larkin J. M., Brown M. S., Goldstein J. L., Anderson R. G. Depletion of intracellular potassium arrests coated pit formation and receptor-mediated endocytosis in fibroblasts. Cell. 1983 May;33(1):273–285. doi: 10.1016/0092-8674(83)90356-2. [DOI] [PubMed] [Google Scholar]
  13. Lowry P. W., McFarland L. M., Threefoot H. K. Vibro hollisae septicemia after consumption of catfish. J Infect Dis. 1986 Oct;154(4):730–731. doi: 10.1093/infdis/154.4.730. [DOI] [PubMed] [Google Scholar]
  14. Miliotis M. D. Acridine orange stain for determining intracellular enteropathogens in HeLa cells. J Clin Microbiol. 1991 Apr;29(4):830–831. doi: 10.1128/jcm.29.4.830-831.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morris J. G., Jr, Miller H. G., Wilson R., Tacket C. O., Hollis D. G., Hickman F. W., Weaver R. E., Blake P. A. Illness caused by Vibrio damsela and Vibrio hollisae. Lancet. 1982 Jun 5;1(8284):1294–1297. doi: 10.1016/s0140-6736(82)92853-7. [DOI] [PubMed] [Google Scholar]
  16. Niesel D. W., Chambers C. E., Stockman S. L. Quantitation of HeLa cell monolayer invasion by Shigella and Salmonella species. J Clin Microbiol. 1985 Dec;22(6):897–902. doi: 10.1128/jcm.22.6.897-902.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nishibuchi M., Doke S., Toizumi S., Umeda T., Yoh M., Miwatani T. Isolation from a coastal fish of Vibrio hollisae capable of producing a hemolysin similar to the thermostable direct hemolysin of Vibrio parahaemolyticus. Appl Environ Microbiol. 1988 Aug;54(8):2144–2146. doi: 10.1128/aem.54.8.2144-2146.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Oelschlaeger T. A., Barrett T. J., Kopecko D. J. Some structures and processes of human epithelial cells involved in uptake of enterohemorrhagic Escherichia coli O157:H7 strains. Infect Immun. 1994 Nov;62(11):5142–5150. doi: 10.1128/iai.62.11.5142-5150.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Oelschlaeger T. A., Guerry P., Kopecko D. J. Unusual microtubule-dependent endocytosis mechanisms triggered by Campylobacter jejuni and Citrobacter freundii. Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6884–6888. doi: 10.1073/pnas.90.14.6884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pearse B. M. Clathrin: a unique protein associated with intracellular transfer of membrane by coated vesicles. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1255–1259. doi: 10.1073/pnas.73.4.1255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Richardson W. P., Sadoff J. C. Induced engulfment of Neisseria gonorrhoeae by tissue culture cells. Infect Immun. 1988 Sep;56(9):2512–2514. doi: 10.1128/iai.56.9.2512-2514.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Small P. L., Isberg R. R., Falkow S. Comparison of the ability of enteroinvasive Escherichia coli, Salmonella typhimurium, Yersinia pseudotuberculosis, and Yersinia enterocolitica to enter and replicate within HEp-2 cells. Infect Immun. 1987 Jul;55(7):1674–1679. doi: 10.1128/iai.55.7.1674-1679.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. St Geme J. W., 3rd, Falkow S. Haemophilus influenzae adheres to and enters cultured human epithelial cells. Infect Immun. 1990 Dec;58(12):4036–4044. doi: 10.1128/iai.58.12.4036-4044.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Söderlund G., Kihlström E. Effect of methylamine and monodansylcadaverine on the susceptibility of McCoy cells to Chlamydia trachomatis infection. Infect Immun. 1983 May;40(2):534–541. doi: 10.1128/iai.40.2.534-541.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vanmaele R. P., Finlayson M. C., Armstrong G. D. Effect of enteropathogenic Escherichia coli on adherent properties of Chinese hamster ovary cells. Infect Immun. 1995 Jan;63(1):191–198. doi: 10.1128/iai.63.1.191-198.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ward M. E., Murray A. Control mechanisms governing the infectivity of Chlamydia trachomatis for HeLa cells: mechanisms of endocytosis. J Gen Microbiol. 1984 Jul;130(7):1765–1780. doi: 10.1099/00221287-130-7-1765. [DOI] [PubMed] [Google Scholar]
  27. Yamamoto T., Kaneko M., Changchawalit S., Serichantalergs O., Ijuin S., Echeverria P. Actin accumulation associated with clustered and localized adherence in Escherichia coli isolated from patients with diarrhea. Infect Immun. 1994 Jul;62(7):2917–2929. doi: 10.1128/iai.62.7.2917-2929.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]

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