Abstract
Recombinant lipopolysaccharide possessing the mannose homopolymer as the O-specific polysaccharide was manufactured genetically by transforming Escherichia coli K-12 with various rfb genes capable of synthesizing the mannose homopolymer. Recombinant lipopolysaccharide exhibited levels of anticomplement activity, adjuvant activity, and regional lymph node-enlarging activity much higher than those exhibited by the original rough-type lipopolysaccharide from E. coli K-12 or lipopolysaccharide possessing the heteropolysaccharide from E. coli O111. Immunological activities of recombinant lipopolysaccharide were as strong as those of wild-type lipopolysaccharide possessing the mannose homopolymer. Characteristic activities of wild-type lipolysaccharide possessing the mannose homopolymer were exhibited by recombinant lipopolysaccharide. The abilities of lipopolysaccharide to activate B cells polyclonally and to produce cytokines did not seem to be related to the presence of the mannose homopolymer. Therefore, it was suggested that the mannose homopolymer in the O-specific polysaccharide might exclusively enhance anticomplement activity, adjuvant activity, and regional lymph node-enlarging activity among various lipid A activities.
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- Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
- Curvall M., Lindberg B., Lönngren J., Nimmich W. Structural studies on the Klebsiella O group 3 lipopolysaccharide. Acta Chem Scand. 1973;27(7):2645–2649. doi: 10.3891/acta.chem.scand.27-2645. [DOI] [PubMed] [Google Scholar]
- Delarue J., Husson M., Schellenberg F., Tichet J., Vol S., Couet C., Lamisse F. Serum lipoprotein(a) [Lp(a)] in alcoholic men: effect of withdrawal. Alcohol. 1996 May-Jun;13(3):309–314. doi: 10.1016/0741-8329(95)02112-4. [DOI] [PubMed] [Google Scholar]
- Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983 Apr;154(1):269–277. doi: 10.1128/jb.154.1.269-277.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ihara S., Takahashi A., Hatsuse H., Sumitomo K., Doi K., Kawakami M. Major component of Ra-reactive factor, a complement-activating bactericidal protein, in mouse serum. J Immunol. 1991 Mar 15;146(6):1874–1879. [PubMed] [Google Scholar]
- Jansson P. E., Lönngren J., Widmalm G., Leontein K., Slettengren K., Svenson S. B., Wrangsell G., Dell A., Tiller P. R. Structural studies of the O-antigen polysaccharides of Klebsiella O5 and Escherichia coli O8. Carbohydr Res. 1985 Dec 15;145(1):59–66. doi: 10.1016/s0008-6215(00)90412-9. [DOI] [PubMed] [Google Scholar]
- Kato N., Ohta M., Kido N., Naito S., Nakashima I., Nagase F., Yokochi T. Strong adjuvanticity of bacterial lipopolysaccharides possessing the homopolysaccharides consisting of mannose as the O-specific polysaccharide chains. Med Microbiol Immunol. 1985;174(1):1–14. [PubMed] [Google Scholar]
- Kido N., Ohta M., Iida K., Hasegawa T., Ito H., Arakawa Y., Komatsu T., Kato N. Partial deletion of the cloned rfb gene of Escherichia coli O9 results in synthesis of a new O-antigenic lipopolysaccharide. J Bacteriol. 1989 Jul;171(7):3629–3633. doi: 10.1128/jb.171.7.3629-3633.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kido N., Ohta M., Ito H., Naito S., Nagase F., Nakashima I., Kato N. Potent adjuvant action of lipopolysaccharides possessing the O-specific polysaccharide moieties consisting of mannans in antibody response against protein antigen. Cell Immunol. 1985 Mar;91(1):52–59. doi: 10.1016/0008-8749(85)90031-0. [DOI] [PubMed] [Google Scholar]
- Kido N., Torgov V. I., Sugiyama T., Uchiya K., Sugihara H., Komatsu T., Kato N., Jann K. Expression of the O9 polysaccharide of Escherichia coli: sequencing of the E. coli O9 rfb gene cluster, characterization of mannosyl transferases, and evidence for an ATP-binding cassette transport system. J Bacteriol. 1995 Apr;177(8):2178–2187. doi: 10.1128/jb.177.8.2178-2187.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morrison D. C., Ryan J. L. Bacterial endotoxins and host immune responses. Adv Immunol. 1979;28:293–450. doi: 10.1016/s0065-2776(08)60802-0. [DOI] [PubMed] [Google Scholar]
- Morrison D. C., Ulevitch R. J. The effects of bacterial endotoxins on host mediation systems. A review. Am J Pathol. 1978 Nov;93(2):526–618. [PMC free article] [PubMed] [Google Scholar]
- Nakashima I., Nagase F., Kato N. Adjuvant action of capsular polysaccharide of Klebsiella pneumoniae on antibody response. VI. Site of its action. Z Immunitatsforsch Immunobiol. 1977 Sep;153(3):204–216. [PubMed] [Google Scholar]
- Ohta M., Kido N., Hasegawa T., Ito H., Fujii Y., Arakawa Y., Komatsu T., Kato N. Contribution of the mannan O side-chains to the adjuvant action of lipopolysaccharides. Immunology. 1987 Apr;60(4):503–507. [PMC free article] [PubMed] [Google Scholar]
- Prehm P., Jann B., Jann K. The O9 antigen of Escherichia coli. Structure of the polysaccharide chain. Eur J Biochem. 1976 Aug 1;67(1):53–56. doi: 10.1111/j.1432-1033.1976.tb10631.x. [DOI] [PubMed] [Google Scholar]
- Rietschel E. T., Brade L., Brandenburg K., Flad H. D., de Jong-Leuveninck J., Kawahara K., Lindner B., Loppnow H., Lüderitz T., Schade U. Chemical structure and biologic activity of bacterial and synthetic lipid A. Rev Infect Dis. 1987 Sep-Oct;9 (Suppl 5):S527–S536. doi: 10.1093/clinids/9.supplement_5.s527. [DOI] [PubMed] [Google Scholar]
- Yokochi T., Fujii Y., Nakashima I., Asai J., Kiuchi M., Kojima K., Kato N. A murine model of experimental autoimmune lens-induced uveitis using Klebsiella O3 lipopolysaccharide as a potent immunological adjuvant. Int J Exp Pathol. 1993 Dec;74(6):573–582. [PMC free article] [PubMed] [Google Scholar]
- Yokochi T., Fukada M., Kawai M., Zhang Y. H., Jiang G. Z., Takahashi K. Novel adjuvant action of lipopolysaccharides that possess mannose homopolysaccharides as O-specific polysaccharides on immune responses to nonimmunogenic autoantigens in mice. Infect Immun. 1992 Nov;60(11):4953–4956. doi: 10.1128/iai.60.11.4953-4956.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yokochi T., Ikeda H., Inoue Y., Kimura Y., Ito H., Fujii Y., Kato N. Characterization of autoantigens relevant to experimental autoimmune orchitis (EAO) in mice immunized with a mixture of syngeneic testis homogenate and Klebsiella O3 lipopolysaccharide. Am J Reprod Immunol. 1990 Jan-Feb;22(1-2):42–48. doi: 10.1111/j.1600-0897.1990.tb01030.x. [DOI] [PubMed] [Google Scholar]
- Yokochi T., Inoue Y., Kimura Y., Kato N. Strong interaction of lipopolysaccharides possessing the mannose homopolysaccharides with complement and its relation to adjuvant action. J Immunol. 1990 Apr 15;144(8):3106–3110. [PubMed] [Google Scholar]
- Yokochi T., Inoue Y., Yokoo J., Kimura Y., Kato N. Retention of bacterial lipopolysaccharide at the site of subcutaneous injection. Infect Immun. 1989 Jun;57(6):1786–1791. doi: 10.1128/iai.57.6.1786-1791.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yokochi T., Nakashima I., Kato N. Adjuvant action of capsular polysaccharide of Klebsiella pneumoniae on antibody response. VIII. Its effect on the size and the number of cells of regional lymph node and other lymphoid organs. Microbiol Immunol. 1980;24(2):141–154. doi: 10.1111/j.1348-0421.1980.tb00572.x. [DOI] [PubMed] [Google Scholar]
- Yokochi T., Nakashima I., Kato N., Asai J., Iijima S. Adjuvant action of capsular polysaccharide of Klebsiella pneumoniae on antibody response. IX. Its effect on the histology of the regional lymph node and other lymphoid organs. Microbiol Immunol. 1980;24(10):933–944. doi: 10.1111/j.1348-0421.1980.tb02899.x. [DOI] [PubMed] [Google Scholar]