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. 1996 Feb;64(2):653–656. doi: 10.1128/iai.64.2.653-656.1996

The N-terminal half of membrane CD14 is a functional cellular lipopolysaccharide receptor.

S Viriyakosol 1, T N Kirkland 1
PMCID: PMC173815  PMID: 8550221

Abstract

CD14, a glycosylphosphatidylinositol-anchored protein on the surface of monocytes, macrophages, and polymorphonuclear leukocytes, is a receptor for lipopolysaccharide (LPS). It was recently reported that an N-terminal 152-amino-acid fragment of soluble CD14 was an active soluble lipopolysaccharide receptor (T. S. -C. Juan, M. J. Kelley, D. A. Johnson, L. A. Busse, E. Hailman, S. D. Wright, and H. S. Lichenstein, J. Biol. Chem. 270:1382-1387, 1995). To determine whether the N-terminal half of the membrane CD14 was a functional LPS receptor on the cell membrane, we engineered a chimeric gene coding for amino acids 1 to 151 of CD14 fused to the C-terminal region of decay-accelerating factor and expressed it in Chinese hamster ovary cells and 70Z/3 cells. We found that the chimeric, truncated CD14 is a fully functional LPS receptor in both cell lines.

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Selected References

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  1. Frey E. A., Miller D. S., Jahr T. G., Sundan A., Bazil V., Espevik T., Finlay B. B., Wright S. D. Soluble CD14 participates in the response of cells to lipopolysaccharide. J Exp Med. 1992 Dec 1;176(6):1665–1671. doi: 10.1084/jem.176.6.1665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gegner J. A., Ulevitch R. J., Tobias P. S. Lipopolysaccharide (LPS) signal transduction and clearance. Dual roles for LPS binding protein and membrane CD14. J Biol Chem. 1995 Mar 10;270(10):5320–5325. doi: 10.1074/jbc.270.10.5320. [DOI] [PubMed] [Google Scholar]
  3. Goyert S. M., Ferrero E., Rettig W. J., Yenamandra A. K., Obata F., Le Beau M. M. The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors. Science. 1988 Jan 29;239(4839):497–500. doi: 10.1126/science.2448876. [DOI] [PubMed] [Google Scholar]
  4. Haziot A., Tsuberi B. Z., Goyert S. M. Neutrophil CD14: biochemical properties and role in the secretion of tumor necrosis factor-alpha in response to lipopolysaccharide. J Immunol. 1993 Jun 15;150(12):5556–5565. [PubMed] [Google Scholar]
  5. Ho S. N., Hunt H. D., Horton R. M., Pullen J. K., Pease L. R. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene. 1989 Apr 15;77(1):51–59. doi: 10.1016/0378-1119(89)90358-2. [DOI] [PubMed] [Google Scholar]
  6. Juan T. S., Hailman E., Kelley M. J., Busse L. A., Davy E., Empig C. J., Narhi L. O., Wright S. D., Lichenstein H. S. Identification of a lipopolysaccharide binding domain in CD14 between amino acids 57 and 64. J Biol Chem. 1995 Mar 10;270(10):5219–5224. doi: 10.1074/jbc.270.10.5219. [DOI] [PubMed] [Google Scholar]
  7. Juan T. S., Kelley M. J., Johnson D. A., Busse L. A., Hailman E., Wright S. D., Lichenstein H. S. Soluble CD14 truncated at amino acid 152 binds lipopolysaccharide (LPS) and enables cellular response to LPS. J Biol Chem. 1995 Jan 20;270(3):1382–1387. doi: 10.1074/jbc.270.3.1382. [DOI] [PubMed] [Google Scholar]
  8. Kirkland T. N., Finley F., Leturcq D., Moriarty A., Lee J. D., Ulevitch R. J., Tobias P. S. Analysis of lipopolysaccharide binding by CD14. J Biol Chem. 1993 Nov 25;268(33):24818–24823. [PubMed] [Google Scholar]
  9. Kirkland T. N., Virca G. D., Kuus-Reichel T., Multer F. K., Kim S. Y., Ulevitch R. J., Tobias P. S. Identification of lipopolysaccharide-binding proteins in 70Z/3 cells by photoaffinity cross-linking. J Biol Chem. 1990 Jun 5;265(16):9520–9525. [PubMed] [Google Scholar]
  10. Kitchens R. L., Ulevitch R. J., Munford R. S. Lipopolysaccharide (LPS) partial structures inhibit responses to LPS in a human macrophage cell line without inhibiting LPS uptake by a CD14-mediated pathway. J Exp Med. 1992 Aug 1;176(2):485–494. doi: 10.1084/jem.176.2.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Koch-Weser J. Bioavailability of drugs (first of two parts). N Engl J Med. 1974 Aug 1;291(5):233–237. doi: 10.1056/NEJM197408012910505. [DOI] [PubMed] [Google Scholar]
  12. Lee J. D., Kato K., Tobias P. S., Kirkland T. N., Ulevitch R. J. Transfection of CD14 into 70Z/3 cells dramatically enhances the sensitivity to complexes of lipopolysaccharide (LPS) and LPS binding protein. J Exp Med. 1992 Jun 1;175(6):1697–1705. doi: 10.1084/jem.175.6.1697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lee J. D., Kravchenko V., Kirkland T. N., Han J., Mackman N., Moriarty A., Leturcq D., Tobias P. S., Ulevitch R. J. Glycosyl-phosphatidylinositol-anchored or integral membrane forms of CD14 mediate identical cellular responses to endotoxin. Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):9930–9934. doi: 10.1073/pnas.90.21.9930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Parrillo J. E., Parker M. M., Natanson C., Suffredini A. F., Danner R. L., Cunnion R. E., Ognibene F. P. Septic shock in humans. Advances in the understanding of pathogenesis, cardiovascular dysfunction, and therapy. Ann Intern Med. 1990 Aug 1;113(3):227–242. doi: 10.7326/0003-4819-113-3-227. [DOI] [PubMed] [Google Scholar]
  15. Pugin J., Ulevitch R. J., Tobias P. S. A critical role for monocytes and CD14 in endotoxin-induced endothelial cell activation. J Exp Med. 1993 Dec 1;178(6):2193–2200. doi: 10.1084/jem.178.6.2193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Raetz C. R., Ulevitch R. J., Wright S. D., Sibley C. H., Ding A., Nathan C. F. Gram-negative endotoxin: an extraordinary lipid with profound effects on eukaryotic signal transduction. FASEB J. 1991 Sep;5(12):2652–2660. doi: 10.1096/fasebj.5.12.1916089. [DOI] [PubMed] [Google Scholar]
  17. Read M. A., Cordle S. R., Veach R. A., Carlisle C. D., Hawiger J. Cell-free pool of CD14 mediates activation of transcription factor NF-kappa B by lipopolysaccharide in human endothelial cells. Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):9887–9891. doi: 10.1073/pnas.90.21.9887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Viriyakosol S., Kirkland T. N. A region of human CD14 required for lipopolysaccharide binding. J Biol Chem. 1995 Jan 6;270(1):361–368. doi: 10.1074/jbc.270.1.361. [DOI] [PubMed] [Google Scholar]
  19. Wright S. D., Ramos R. A., Tobias P. S., Ulevitch R. J., Mathison J. C. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 1990 Sep 21;249(4975):1431–1433. doi: 10.1126/science.1698311. [DOI] [PubMed] [Google Scholar]

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