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. 1996 Feb;64(2):657–659. doi: 10.1128/iai.64.2.657-659.1996

Structural characteristics of peptidoglycan fragments required to prime mice for induction of anaphylactoid reactions by lipopolysaccharides.

H Takada 1, Y Kawabata 1, S Kawata 1, S Kusumoto 1
PMCID: PMC173816  PMID: 8550222

Abstract

Structural characteristics of peptidoglycan fragments required to prime mice for the induction of anaphylactoid reactions by Salmonella abortusequi lipopolysaccharide were examined in endotoxin-resistant C3H/HeJ mice, with special focus on the disaccharide-pentapeptide [N-acetylglucosaminyl-beta(1-4)-N-acetylmuramyl-L-alanyl-D -isoglutaminyl-meso-2,6-diaminopimelyl (DAP)-D-alanyl-D -alanine] and its smaller partial derivatives. The bacterial and synthetic muramyl tripeptides (DAP- and lysine [Lys]-type, respectively) and synthetic muramyl dipeptide primed mice for induction of anaphylactoid reactions accompanied by death within 1 h. The disaccharide-tripeptide exhibited weaker activity, and the disaccharide-tetrapeptide and muramyl tetrapeptide exhibited marginal activity. In contrast, intact peptidoglycans of various bacteria and the disaccharide-pentapeptide lacked the priming activity, although they showed adjuvant activity similar to that of the above components.

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Selected References

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  1. Galanos C., Freudenberg M. A., Matsuura M. Mechanisms of the lethal action of endotoxin and endotoxin hypersensitivity. Adv Exp Med Biol. 1990;256:603–619. doi: 10.1007/978-1-4757-5140-6_56. [DOI] [PubMed] [Google Scholar]
  2. Galanos C., Lüderitz O., Westphal O. Preparation and properties of a standardized lipopolysaccharide from salmonella abortus equi (Novo-Pyrexal). Zentralbl Bakteriol Orig A. 1979 Apr;243(2-3):226–244. [PubMed] [Google Scholar]
  3. Gotoh T., Nakahara K., Nishiura T., Hashimoto M., Kino T., Kuroda Y., Okuhara M., Kohsaka M., Aoki H., Imanaka H. Studies on a new immunoactive peptide, FK-156. II. Fermentation, extraction and chemical and biological characterization. J Antibiot (Tokyo) 1982 Oct;35(10):1286–1292. doi: 10.7164/antibiotics.35.1286. [DOI] [PubMed] [Google Scholar]
  4. Johannsen L., Wecke J., Obál F., Jr, Krueger J. M. Macrophages produce somnogenic and pyrogenic muramyl peptides during digestion of staphylococci. Am J Physiol. 1991 Jan;260(1 Pt 2):R126–R133. doi: 10.1152/ajpregu.1991.260.1.R126. [DOI] [PubMed] [Google Scholar]
  5. Kawasaki A., Takada H., Kotani S., Inai S., Nagaki K., Matsumoto M., Yokogawa K., Kawata S., Kusumoto S., Shiba T. Activation of the human complement cascade by bacterial cell walls, peptidoglycans, water-soluble peptidoglycan components, and synthetic muramylpeptides--studies on active components and structural requirements. Microbiol Immunol. 1987;31(6):551–569. doi: 10.1111/j.1348-0421.1987.tb03117.x. [DOI] [PubMed] [Google Scholar]
  6. Keglević D., Ladesić B., Tomasić J., Valinger Z., Naumski R. Isolation procedure and properties of monomer unit from lysozyme digest of peptidoglycan complex excreted into the medium by penicillin-treated Brevibacterium divaricatum mutant. Biochim Biophys Acta. 1979 Jun 12;585(2):273–281. doi: 10.1016/0304-4165(79)90027-8. [DOI] [PubMed] [Google Scholar]
  7. Nagao S., Kawabata Y., Kitano M., Suzuki K., Nishikawa T., Takada H. Modification of delayed-type hypersensitivity reactions by muramyldipeptide in guinea pigs. FEMS Immunol Med Microbiol. 1995 Jun;11(3):231–245. doi: 10.1111/j.1574-695X.1995.tb00121.x. [DOI] [PubMed] [Google Scholar]
  8. Nagao S., Takada H., Yagawa K., Kutsukake H., Shiba T., Kusumoto S., Kawata S., Hasegawa A., Kiso M., Azuma I. Structural requirements of muramylpeptides for induction of necrosis at sites primed with Mycobacterium tuberculosis in guinea pigs. Infect Immun. 1987 May;55(5):1279–1288. doi: 10.1128/iai.55.5.1279-1288.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ribi E. E., Cantrell J. L., Von Eschen K. B., Schwartzman S. M. Enhancement of endotoxic shock by N-acetylmuramyl-L-alanyl-(L-seryl)-D-isoglutamine (muramyl dipeptide). Cancer Res. 1979 Nov;39(11):4756–4759. [PubMed] [Google Scholar]
  10. Takada H., Galanos C. Enhancement of endotoxin lethality and generation of anaphylactoid reactions by lipopolysaccharides in muramyl-dipeptide-treated mice. Infect Immun. 1987 Feb;55(2):409–413. doi: 10.1128/iai.55.2.409-413.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Takada H., Hirai H., Fujiwara T., Koga T., Ogawa T., Hamada S. Bacteroides lipopolysaccharides (LPS) induce anaphylactoid and lethal reactions in LPS-responsive and -nonresponsive mice primed with muramyl dipeptide. J Infect Dis. 1990 Aug;162(2):428–434. doi: 10.1093/infdis/162.2.428. [DOI] [PubMed] [Google Scholar]
  12. Takada H., Tsujimoto M., Kotani S., Kusumoto S., Inage M., Shiba T., Nagao S., Yano I., Kawata S., Yokogawa K. Mitogenic effects of bacterial cell walls, their fragments, and related synthetic compounds on thymocytes and splenocytes of guinea pigs. Infect Immun. 1979 Aug;25(2):645–652. doi: 10.1128/iai.25.2.645-652.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vermeulen M. W., Gray G. R. Processing of Bacillus subtilis peptidoglycan by a mouse macrophage cell line. Infect Immun. 1984 Nov;46(2):476–483. doi: 10.1128/iai.46.2.476-483.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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