Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1996 Mar;64(3):995–1001. doi: 10.1128/iai.64.3.995-1001.1996

Antibodies to Haemophilus influenzae type b polysaccharide affect bacterial adherence and multiplication.

L van Alphen 1, P Eijk 1, H Käyhty 1, J van Marle 1, J Dankert 1
PMCID: PMC173868  PMID: 8641812

Abstract

Since immunization of infants with conjugated Haemophilus influenzae type b (Hib) capsular polysaccharide (PS) vaccines results in a reduction of colonization, we determined the inhibitory effect of anti-Hib PS on two steps in the colonization, i.e., adherence of H. influenzae to nasopharyngeal epithelium and bacterial growth. Monoclonal antibody (MAb) E117-5 specific for Hib PS inhibited at a concentration of at least 80 microg/ml in vitro the adherence of Hib strain 770235f+b+ to oropharyngeal epithelial cells by 50% (P <, 0.02), but this MAb and sera from children immunized with Hib PS conjugate vaccine (n = 10) were not inhibitory in final dilutions containing up to 20 microg of anti-Hib PS per ml. The growth of Hib strain 770235f+b+ did completely stop in the presence of 5 microg of anti-Hib PS MAb E117-5 per ml and human sera with an anti-Hib PS concentration of 2 microg/ml or more, in contrast to the growth of the nonencapsular variant strain 770235f+b0.

Full Text

The Full Text of this article is available as a PDF (271.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banks-Schlegel S. P., Gazdar A. F., Harris C. C. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 1985 Mar;45(3):1187–1197. [PubMed] [Google Scholar]
  2. Barenkamp S. J., Leininger E. Cloning, expression, and DNA sequence analysis of genes encoding nontypeable Haemophilus influenzae high-molecular-weight surface-exposed proteins related to filamentous hemagglutinin of Bordetella pertussis. Infect Immun. 1992 Apr;60(4):1302–1313. doi: 10.1128/iai.60.4.1302-1313.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dirks-Go S. I., Zanen H. C. Latex agglutination, counterimmunoelectrophoresis, and protein A co-agglutination in diagnosis of bacterial meningitis. J Clin Pathol. 1978 Dec;31(12):1167–1171. doi: 10.1136/jcp.31.12.1167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Forsgren J., Samuelson A., Ahlin A., Jonasson J., Rynnel-Dagö B., Lindberg A. Haemophilus influenzae resides and multiplies intracellularly in human adenoid tissue as demonstrated by in situ hybridization and bacterial viability assay. Infect Immun. 1994 Feb;62(2):673–679. doi: 10.1128/iai.62.2.673-679.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harabuchi Y., Faden H., Yamanaka N., Duffy L., Wolf J., Krystofik D. Nasopharyngeal colonization with nontypeable Haemophilus influenzae and recurrent otitis media. Tonawanda/Williamsville Pediatrics. J Infect Dis. 1994 Oct;170(4):862–866. doi: 10.1093/infdis/170.4.862. [DOI] [PubMed] [Google Scholar]
  6. Kauppi M., Saarinen L., Käyhty H. Anti-capsular polysaccharide antibodies reduce nasopharyngeal colonization by Haemophilus influenzae type b in infant rats. J Infect Dis. 1993 Feb;167(2):365–371. doi: 10.1093/infdis/167.2.365. [DOI] [PubMed] [Google Scholar]
  7. Käyhty H., Eskola J., Peltola H., Saarinen L., Mäkelä P. H. High antibody responses to booster doses of either Haemophilus influenzae capsular polysaccharide or conjugate vaccine after primary immunization with conjugate vaccines. J Infect Dis. 1992 Jun;165 (Suppl 1):S165–S166. doi: 10.1093/infdis/165-supplement_1-s165. [DOI] [PubMed] [Google Scholar]
  8. Loeb M. R., Connor E., Penney D. A comparison of the adherence of fimbriated and nonfimbriated Haemophilus influenzae type b to human adenoids in organ culture. Infect Immun. 1988 Feb;56(2):484–489. doi: 10.1128/iai.56.2.484-489.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Murphy T. V., Pastor P., Medley F., Osterholm M. T., Granoff D. M. Decreased Haemophilus colonization in children vaccinated with Haemophilus influenzae type b conjugate vaccine. J Pediatr. 1993 Apr;122(4):517–523. doi: 10.1016/s0022-3476(05)83529-2. [DOI] [PubMed] [Google Scholar]
  10. Noel G. J., Mosser D. M., Edelson P. J. Role of complement in mouse macrophage binding of Haemophilus influenzae type b. J Clin Invest. 1990 Jan;85(1):208–218. doi: 10.1172/JCI114414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Peltola H., Kilpi T., Anttila M. Rapid disappearance of Haemophilus influenzae type b meningitis after routine childhood immunisation with conjugate vaccines. Lancet. 1992 Sep 5;340(8819):592–594. doi: 10.1016/0140-6736(92)92117-x. [DOI] [PubMed] [Google Scholar]
  12. Robbins J. B., Schneerson R., Szu S. C. Perspective: hypothesis: serum IgG antibody is sufficient to confer protection against infectious diseases by inactivating the inoculum. J Infect Dis. 1995 Jun;171(6):1387–1398. doi: 10.1093/infdis/171.6.1387. [DOI] [PubMed] [Google Scholar]
  13. Rutter J. M., Jones G. W. Protection against enteric disease caused by Escherichia coli--a model for vaccination with a virulence determinant? Nature. 1973 Apr 20;242(5399):531–532. doi: 10.1038/242531a0. [DOI] [PubMed] [Google Scholar]
  14. Schneerson R., Barrera O., Sutton A., Robbins J. B. Preparation, characterization, and immunogenicity of Haemophilus influenzae type b polysaccharide-protein conjugates. J Exp Med. 1980 Aug 1;152(2):361–376. doi: 10.1084/jem.152.2.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Takala A. K., Eskola J., Leinonen M., Käyhty H., Nissinen A., Pekkanen E., Mäkelä P. H. Reduction of oropharyngeal carriage of Haemophilus influenzae type b (Hib) in children immunized with an Hib conjugate vaccine. J Infect Dis. 1991 Nov;164(5):982–986. doi: 10.1093/infdis/164.5.982. [DOI] [PubMed] [Google Scholar]
  16. Takala A. K., Santosham M., Almeido-Hill J., Wolff M., Newcomer W., Reid R., Käyhty H., Esko E., Mäkelä P. H. Vaccination with Haemophilus influenzae type b meningococcal protein conjugate vaccine reduces oropharyngeal carriage of Haemophilus influenzae type b among American Indian children. Pediatr Infect Dis J. 1993 Jul;12(7):593–599. doi: 10.1097/00006454-199307000-00010. [DOI] [PubMed] [Google Scholar]
  17. Vogel L., van Alphen L., Geluk F., Troelstra A., Martin E., Bredius R., Eijk P., Jansen H., Dankert J. Quantitative flow cytometric analysis of opsonophagocytosis and killing of nonencapsulated Haemophilus influenzae by human polymorphonuclear leukocytes. Clin Diagn Lab Immunol. 1994 Jul;1(4):394–400. doi: 10.1128/cdli.1.4.394-400.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weber A., Harris K., Lohrke S., Forney L., Smith A. L. Inability to express fimbriae results in impaired ability of Haemophilus influenzae b to colonize the nasopharynx. Infect Immun. 1991 Dec;59(12):4724–4728. doi: 10.1128/iai.59.12.4724-4728.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. van Alphen L., Eijk P., Geelen-van den Broek L., Dankert J. Immunochemical characterization of variable epitopes of outer membrane protein P2 of nontypeable Haemophilus influenzae. Infect Immun. 1991 Jan;59(1):247–252. doi: 10.1128/iai.59.1.247-252.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. van Alphen L., Geelen-van den Broek L., Blaas L., van Ham M., Dankert J. Blocking of fimbria-mediated adherence of Haemophilus influenzae by sialyl gangliosides. Infect Immun. 1991 Dec;59(12):4473–4477. doi: 10.1128/iai.59.12.4473-4477.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. van Alphen L., Geelen-van den Broek L., van Ham M. In vivo and in vitro expression of outer membrane components of Haemophilus influenzae. Microb Pathog. 1990 Apr;8(4):279–288. doi: 10.1016/0882-4010(90)90053-s. [DOI] [PubMed] [Google Scholar]
  22. van Alphen L., van den Berghe N., Geelen-van den Broek L. Interaction of Haemophilus influenzae with human erythrocytes and oropharyngeal epithelial cells is mediated by a common fimbrial epitope. Infect Immun. 1988 Jul;56(7):1800–1806. doi: 10.1128/iai.56.7.1800-1806.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. van Ham S. M., van Alphen L., Mooi F. R., van Putten J. P. Phase variation of H. influenzae fimbriae: transcriptional control of two divergent genes through a variable combined promoter region. Cell. 1993 Jun 18;73(6):1187–1196. doi: 10.1016/0092-8674(93)90647-9. [DOI] [PubMed] [Google Scholar]
  24. van Ham S. M., van Alphen L., Mooi F. R., van Putten J. P. The fimbrial gene cluster of Haemophilus influenzae type b. Mol Microbiol. 1994 Aug;13(4):673–684. doi: 10.1111/j.1365-2958.1994.tb00461.x. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES