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. 1996 Mar;64(3):1016–1019. doi: 10.1128/iai.64.3.1016-1019.1996

Translocation of Yersinia enterocolitica through an endothelial monolayer by polymorphonuclear leukocytes.

H Rüssmann 1, K Ruckdeschel 1, J Heesemann 1
PMCID: PMC173872  PMID: 8641751

Abstract

An endothelial cell monolayer grown on a microporous membrane coated with basement membrane protein matrix was used to study translocation of yersinia-infected human polymorphonuclear leukocytes (PMNs). PMNs infected with one to eight bacteria were able to translocate living yersiniae from the upper chamber to the chemoattractant-containing lower chamber. This process may contribute to extravasation and dissemination of yersiniae in the infected host.

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Selected References

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  1. Bliska J. B., Clemens J. C., Dixon J. E., Falkow S. The Yersinia tyrosine phosphatase: specificity of a bacterial virulence determinant for phosphoproteins in the J774A.1 macrophage. J Exp Med. 1992 Dec 1;176(6):1625–1630. doi: 10.1084/jem.176.6.1625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brubaker R. R. Factors promoting acute and chronic diseases caused by yersiniae. Clin Microbiol Rev. 1991 Jul;4(3):309–324. doi: 10.1128/cmr.4.3.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. China B., Sory M. P., N'Guyen B. T., De Bruyere M., Cornelis G. R. Role of the YadA protein in prevention of opsonization of Yersinia enterocolitica by C3b molecules. Infect Immun. 1993 Aug;61(8):3129–3136. doi: 10.1128/iai.61.8.3129-3136.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cover T. L., Aber R. C. Yersinia enterocolitica. N Engl J Med. 1989 Jul 6;321(1):16–24. doi: 10.1056/NEJM198907063210104. [DOI] [PubMed] [Google Scholar]
  5. Devenish J. A., Schiemann D. A. HeLa cell infection by Yersinia enterocolitica: evidence for lack of intracellular multiplication and development of a new procedure for quantitative expression of infectivity. Infect Immun. 1981 Apr;32(1):48–55. doi: 10.1128/iai.32.1.48-55.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dunlap N. E., Benjamin W. H., Jr, Berry A. K., Eldridge J. H., Briles D. E. A 'safe-site' for Salmonella typhimurium is within splenic polymorphonuclear cells. Microb Pathog. 1992 Sep;13(3):181–190. doi: 10.1016/0882-4010(92)90019-k. [DOI] [PubMed] [Google Scholar]
  7. Ewald J. H., Heesemann J., Rüdiger H., Autenrieth I. B. Interaction of polymorphonuclear leukocytes with Yersinia enterocolitica: role of the Yersinia virulence plasmid and modulation by the iron-chelator desferrioxamine B. J Infect Dis. 1994 Jul;170(1):140–150. doi: 10.1093/infdis/170.1.140. [DOI] [PubMed] [Google Scholar]
  8. Grützkau A., Hanski C., Hahn H., Riecken E. O. Involvement of M cells in the bacterial invasion of Peyer's patches: a common mechanism shared by Yersinia enterocolitica and other enteroinvasive bacteria. Gut. 1990 Sep;31(9):1011–1015. doi: 10.1136/gut.31.9.1011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hammer M., Zeidler H., Klimsa S., Heesemann J. Yersinia enterocolitica in the synovial membrane of patients with Yersinia-induced arthritis. Arthritis Rheum. 1990 Dec;33(12):1795–1800. doi: 10.1002/art.1780331206. [DOI] [PubMed] [Google Scholar]
  10. Heesemann J., Gaede K., Autenrieth I. B. Experimental Yersinia enterocolitica infection in rodents: a model for human yersiniosis. APMIS. 1993 Jun;101(6):417–429. [PubMed] [Google Scholar]
  11. Heesemann J., Laufs R. Double immunofluorescence microscopic technique for accurate differentiation of extracellularly and intracellularly located bacteria in cell culture. J Clin Microbiol. 1985 Aug;22(2):168–175. doi: 10.1128/jcm.22.2.168-175.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pepe J. C., Miller V. L. Yersinia enterocolitica invasin: a primary role in the initiation of infection. Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6473–6477. doi: 10.1073/pnas.90.14.6473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Riley B. S., Oppenheimer-Marks N., Hansen E. J., Radolf J. D., Norgard M. V. Virulent Treponema pallidum activates human vascular endothelial cells. J Infect Dis. 1992 Mar;165(3):484–493. doi: 10.1093/infdis/165.3.484. [DOI] [PubMed] [Google Scholar]
  14. Rosqvist R., Magnusson K. E., Wolf-Watz H. Target cell contact triggers expression and polarized transfer of Yersinia YopE cytotoxin into mammalian cells. EMBO J. 1994 Feb 15;13(4):964–972. doi: 10.1002/j.1460-2075.1994.tb06341.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ruckdeschel K., Roggenkamp A., Schubert S., Heesemann J. Differential contribution of Yersinia enterocolitica virulence factors to evasion of microbicidal action of neutrophils. Infect Immun. 1996 Mar;64(3):724–733. doi: 10.1128/iai.64.3.724-733.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Simonet M., Richard S., Berche P. Electron microscopic evidence for in vivo extracellular localization of Yersinia pseudotuberculosis harboring the pYV plasmid. Infect Immun. 1990 Mar;58(3):841–845. doi: 10.1128/iai.58.3.841-845.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Straley S. C., Skrzypek E., Plano G. V., Bliska J. B. Yops of Yersinia spp. pathogenic for humans. Infect Immun. 1993 Aug;61(8):3105–3110. doi: 10.1128/iai.61.8.3105-3110.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wells C. L., Maddaus M. A., Simmons R. L. Proposed mechanisms for the translocation of intestinal bacteria. Rev Infect Dis. 1988 Sep-Oct;10(5):958–979. doi: 10.1093/clinids/10.5.958. [DOI] [PubMed] [Google Scholar]

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