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. 1996 Mar;64(3):1030–1034. doi: 10.1128/iai.64.3.1030-1034.1996

Antibody responses to Brucella abortus 2308 in cattle vaccinated with B. abortus RB51.

M G Stevens 1, S C Olsen 1
PMCID: PMC173875  PMID: 8641754

Abstract

Cattle vaccinated with Brucella abortus rough strain RB51 (SRB51) produced small amounts of serum immunoglobulin G (IgG) but no IgM antibody to smooth strain 2308 (S2308) bacteria and produced no IgG or IgM antibody to S2308 lipopolysaccharide (LPS). Western immunoblot analysis revealed that antiserum from SRB51-vaccinated cattle contained IgG antibody that reacted with S2308 proteins of 84 to <20 kDa. However, antiserum from the vaccinated cattle did not contain agglutinating B. abortus antibody in the tube agglutination test for brucellosis. These results suggest that SRB51-vaccinated cattle produced no antibody to S2308 LPS, although they did produce nonagglutinating IgG antibody that reacted with S2308 bacteria and bacterial proteins of 84 to <20 kDa.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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