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. 1996 Apr;64(4):1467–1472. doi: 10.1128/iai.64.4.1467-1472.1996

Construction and preliminary characterization of three hemagglutinin mutants of Porphyromonas gingivalis.

G Lépine 1, R P Ellen 1, A Progulske-Fox 1
PMCID: PMC173946  PMID: 8606121

Abstract

Targeted insertional mutagenesis was used to construct hagA, hagB, and hagC hemagglutinin mutants of Porphyromonas gingivalis. pJRD215-derived plasmids containing tetA(Q)2 and portions of the targeted genes were conjugated into P. gingivalis. Interruption of the three loci was confirmed by Southern hybridization, sequencing, reverse transcription-PCR, and microtiter hemagglutination assays. No significant differences in hydrophobicity or coadherence to Actinomyces viscosus were detected between the mutants and the wild-type strain.

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Selected References

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