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. 1996 Apr;64(4):1484–1487. doi: 10.1128/iai.64.4.1484-1487.1996

Experimental Yersinia infection of human synovial cells: persistence of live bacteria and generation of bacterial antigen deposits including "ghosts," nucleic acid-free bacterial rods.

H I Huppertz 1, J Heesemann 1
PMCID: PMC173950  PMID: 8606125

Abstract

Yersinia enterocolitica O:3 was maintained in primary cultures of human synovial cells for 6 weeks as cultivable organisms and thereafter for 2 more weeks as antigen aggregates containing specific lipopolysaccharides (LPS). Some seemingly intact bacteria were "ghosts," bacterial rods possessing LPS but not DNA. The prolonged persistence of yersiniae, and consequently of Yersinia antigens, in synovial cells may be the cause of the maintenance of the inflammatory host responses in the joints of patients with reactive arthritis due to Yersinia infection.

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Selected References

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  1. Devenish J. A., Schiemann D. A. HeLa cell infection by Yersinia enterocolitica: evidence for lack of intracellular multiplication and development of a new procedure for quantitative expression of infectivity. Infect Immun. 1981 Apr;32(1):48–55. doi: 10.1128/iai.32.1.48-55.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ewald J. H., Heesemann J., Rüdiger H., Autenrieth I. B. Interaction of polymorphonuclear leukocytes with Yersinia enterocolitica: role of the Yersinia virulence plasmid and modulation by the iron-chelator desferrioxamine B. J Infect Dis. 1994 Jul;170(1):140–150. doi: 10.1093/infdis/170.1.140. [DOI] [PubMed] [Google Scholar]
  3. Gaede K. I., Heesemann J. Arthritogenicity of genetically manipulated Yersinia enterocolitica serotype O8 for Lewis rats. Infect Immun. 1995 Feb;63(2):714–719. doi: 10.1128/iai.63.2.714-719.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Granfors K., Jalkanen S., von Essen R., Lahesmaa-Rantala R., Isomäki O., Pekkola-Heino K., Merilahti-Palo R., Saario R., Isomäki H., Toivanen A. Yersinia antigens in synovial-fluid cells from patients with reactive arthritis. N Engl J Med. 1989 Jan 26;320(4):216–221. doi: 10.1056/NEJM198901263200404. [DOI] [PubMed] [Google Scholar]
  5. Hammer M., Zeidler H., Klimsa S., Heesemann J. Yersinia enterocolitica in the synovial membrane of patients with Yersinia-induced arthritis. Arthritis Rheum. 1990 Dec;33(12):1795–1800. doi: 10.1002/art.1780331206. [DOI] [PubMed] [Google Scholar]
  6. Heesemann J., Gaede K., Autenrieth I. B. Experimental Yersinia enterocolitica infection in rodents: a model for human yersiniosis. APMIS. 1993 Jun;101(6):417–429. [PubMed] [Google Scholar]
  7. Heesemann J., Laufs R. Double immunofluorescence microscopic technique for accurate differentiation of extracellularly and intracellularly located bacteria in cell culture. J Clin Microbiol. 1985 Aug;22(2):168–175. doi: 10.1128/jcm.22.2.168-175.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huppertz H. I., Chantler J. K. Restricted mumps virus infection of cells derived from normal human joint tissue. J Gen Virol. 1991 Feb;72(Pt 2):339–347. doi: 10.1099/0022-1317-72-2-339. [DOI] [PubMed] [Google Scholar]
  9. Huppertz H. I. How could infectious agents hide in synovial cells? Possible mechanisms of persistent viral infection in a model for the etiopathogenesis of chronic arthritis. Rheumatol Int. 1994;14(2):71–75. doi: 10.1007/BF00300250. [DOI] [PubMed] [Google Scholar]
  10. Huppertz H. I., Niki N. P., Chantler J. K. Susceptibility of normal human joint tissue to viruses. J Rheumatol. 1991 May;18(5):699–704. [PubMed] [Google Scholar]
  11. Jernaes M. W., Steen H. B. Staining of Escherichia coli for flow cytometry: influx and efflux of ethidium bromide. Cytometry. 1994 Dec 1;17(4):302–309. doi: 10.1002/cyto.990170405. [DOI] [PubMed] [Google Scholar]
  12. Leirisalo-Repo M., Suoranta H. Ten-year follow-up study of patients with Yersinia arthritis. Arthritis Rheum. 1988 Apr;31(4):533–537. doi: 10.1002/art.1780310410. [DOI] [PubMed] [Google Scholar]
  13. Merilahti-Palo R., Söderström K. O., Lahesmaa-Rantala R., Granfors K., Toivanen A. Bacterial antigens in synovial biopsy specimens in yersinia triggered reactive arthritis. Ann Rheum Dis. 1991 Feb;50(2):87–90. doi: 10.1136/ard.50.2.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Valtonen V. V., Leirisalo M., Pentikäinen P. J., Räsänen T., Seppälä I., Larinkari U., Ranki M., Koskimies S., Malkamäki M., Mäkelä P. H. Triggering infections in reactive arthritis. Ann Rheum Dis. 1985 Jun;44(6):399–405. doi: 10.1136/ard.44.6.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Viitanen A. M., Arstila T. P., Lahesmaa R., Granfors K., Skurnik M., Toivanen P. Application of the polymerase chain reaction and immunofluorescence techniques to the detection of bacteria in Yersinia-triggered reactive arthritis. Arthritis Rheum. 1991 Jan;34(1):89–96. doi: 10.1002/art.1780340114. [DOI] [PubMed] [Google Scholar]
  16. Wuorela M., Jalkanen S., Toivanen P., Granfors K. Yersinia lipopolysaccharide is modified by human monocytes. Infect Immun. 1993 Dec;61(12):5261–5270. doi: 10.1128/iai.61.12.5261-5270.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. de Koning J., Heesemann J., Hoogkamp-Korstanje J. A., Festen J. J., Houtman P. M., van Oijen P. L. Yersinia in intestinal biopsy specimens from patients with seronegative spondyloarthropathy: correlation with specific serum IgA antibodies. J Infect Dis. 1989 Jan;159(1):109–112. doi: 10.1093/infdis/159.1.109. [DOI] [PubMed] [Google Scholar]

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