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. 1996 May;64(5):1846–1849. doi: 10.1128/iai.64.5.1846-1849.1996

Streptococcus mitis cell walls and lipopolysaccharide induce lethality in D-galactosamine-sensitized mice by a tumor necrosis factor-dependent pathway.

D Le Roy 1, P Morand 1, S Lengacher 1, M Celio 1, G E Grau 1, M P Glauser 1, D Heumann 1
PMCID: PMC174002  PMID: 8613401

Abstract

Purified cells walls of Streptococcus mitis induced tumor necrosis factor in vitro in whole blood of both lipopolysaccharide (LPS)-sensitive OF1 and LPS-resistant C3H/HeJ mice. They were as effective as heat-killed bacteria in inducing death in both strains of mice sensitized with D-galactosamine. Lethality was suppressed by anti-tumor necrosis factor antibodies. The histopathophysiological findings in mice after challenge with LPS or gram-positive cell walls were indistinguishable.

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Selected References

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  1. Awada A., van der Auwera P., Meunier F., Daneau D., Klastersky J. Streptococcal and enterococcal bacteremia in patients with cancer. Clin Infect Dis. 1992 Jul;15(1):33–48. doi: 10.1093/clinids/15.1.33. [DOI] [PubMed] [Google Scholar]
  2. Bhakdi S., Klonisch T., Nuber P., Fischer W. Stimulation of monokine production by lipoteichoic acids. Infect Immun. 1991 Dec;59(12):4614–4620. doi: 10.1128/iai.59.12.4614-4620.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bochud P. Y., Eggiman P., Calandra T., Van Melle G., Saghafi L., Francioli P. Bacteremia due to viridans streptococcus in neutropenic patients with cancer: clinical spectrum and risk factors. Clin Infect Dis. 1994 Jan;18(1):25–31. doi: 10.1093/clinids/18.1.25. [DOI] [PubMed] [Google Scholar]
  4. Bone R. C. Gram-positive organisms and sepsis. Arch Intern Med. 1994 Jan 10;154(1):26–34. [PubMed] [Google Scholar]
  5. Bonventre P. F., Heeg H., Cullen C., Lian C. J. Toxicity of recombinant toxic shock syndrome toxin 1 and mutant toxins produced by Staphylococcus aureus in a rabbit infection model of toxic shock syndrome. Infect Immun. 1993 Mar;61(3):793–799. doi: 10.1128/iai.61.3.793-799.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cavaillon J. M., Haeffner-Cavaillon N. Polymyxin-B inhibition of LPS-induced interleukin-1 secretion by human monocytes is dependent upon the LPS origin. Mol Immunol. 1986 Sep;23(9):965–969. doi: 10.1016/0161-5890(86)90127-6. [DOI] [PubMed] [Google Scholar]
  7. Cohen J., Donnelly J. P., Worsley A. M., Catovsky D., Goldman J. M., Galton D. A. Septicaemia caused by viridans streptococci in neutropenic patients with leukaemia. Lancet. 1983 Dec 24;2(8365-66):1452–1454. doi: 10.1016/s0140-6736(83)90799-7. [DOI] [PubMed] [Google Scholar]
  8. Elting L. S., Bodey G. P., Keefe B. H. Septicemia and shock syndrome due to viridans streptococci: a case-control study of predisposing factors. Clin Infect Dis. 1992 Jun;14(6):1201–1207. doi: 10.1093/clinids/14.6.1201. [DOI] [PubMed] [Google Scholar]
  9. Freudenberg M. A., Galanos C. Tumor necrosis factor alpha mediates lethal activity of killed gram-negative and gram-positive bacteria in D-galactosamine-treated mice. Infect Immun. 1991 Jun;59(6):2110–2115. doi: 10.1128/iai.59.6.2110-2115.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Galanos C., Freudenberg M. A., Reutter W. Galactosamine-induced sensitization to the lethal effects of endotoxin. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5939–5943. doi: 10.1073/pnas.76.11.5939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Heumann D., Barras C., Severin A., Glauser M. P., Tomasz A. Gram-positive cell walls stimulate synthesis of tumor necrosis factor alpha and interleukin-6 by human monocytes. Infect Immun. 1994 Jul;62(7):2715–2721. doi: 10.1128/iai.62.7.2715-2721.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Keller R., Fischer W., Keist R., Bassetti S. Macrophage response to bacteria: induction of marked secretory and cellular activities by lipoteichoic acids. Infect Immun. 1992 Sep;60(9):3664–3672. doi: 10.1128/iai.60.9.3664-3672.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lehmann V., Freudenberg M. A., Galanos C. Lethal toxicity of lipopolysaccharide and tumor necrosis factor in normal and D-galactosamine-treated mice. J Exp Med. 1987 Mar 1;165(3):657–663. doi: 10.1084/jem.165.3.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Matsushita K., Fujimaki W., Kato H., Uchiyama T., Igarashi H., Ohkuni H., Nagaoka S., Kawagoe M., Kotani S., Takada H. Immunopathological activities of extracellular products of Streptococcus mitis, particularly a superantigenic fraction. Infect Immun. 1995 Mar;63(3):785–793. doi: 10.1128/iai.63.3.785-793.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mattsson E., Rollof J., Verhoef J., Van Dijk H., Fleer A. Serum-induced potentiation of tumor necrosis factor alpha production by human monocytes in response to staphylococcal peptidoglycan: involvement of different serum factors. Infect Immun. 1994 Sep;62(9):3837–3843. doi: 10.1128/iai.62.9.3837-3843.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Miethke T., Wahl C., Heeg K., Echtenacher B., Krammer P. H., Wagner H. T cell-mediated lethal shock triggered in mice by the superantigen staphylococcal enterotoxin B: critical role of tumor necrosis factor. J Exp Med. 1992 Jan 1;175(1):91–98. doi: 10.1084/jem.175.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Morrison D. C., Jacobs D. M. Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. Immunochemistry. 1976 Oct;13(10):813–818. doi: 10.1016/0019-2791(76)90181-6. [DOI] [PubMed] [Google Scholar]
  18. Natanson C., Danner R. L., Elin R. J., Hosseini J. M., Peart K. W., Banks S. M., MacVittie T. J., Walker R. I., Parrillo J. E. Role of endotoxemia in cardiovascular dysfunction and mortality. Escherichia coli and Staphylococcus aureus challenges in a canine model of human septic shock. J Clin Invest. 1989 Jan;83(1):243–251. doi: 10.1172/JCI113866. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pratter M. R., Irwin R. S. Viridans streptococcal pulmonary parenchymal infections. JAMA. 1980 Jun 27;243(24):2515–2517. [PubMed] [Google Scholar]
  20. Pugin J., Heumann I. D., Tomasz A., Kravchenko V. V., Akamatsu Y., Nishijima M., Glauser M. P., Tobias P. S., Ulevitch R. J. CD14 is a pattern recognition receptor. Immunity. 1994 Sep;1(6):509–516. doi: 10.1016/1074-7613(94)90093-0. [DOI] [PubMed] [Google Scholar]
  21. ROBERSON B. S., SCHWAB J. H. Endotoxic properties associated with cell walls of group A streptococci. J Infect Dis. 1961 Jan-Feb;108:25–34. doi: 10.1093/infdis/108.1.25. [DOI] [PubMed] [Google Scholar]
  22. Remick D. G., Strieter R. M., Eskandari M. K., Nguyen D. T., Genord M. A., Raiford C. L., Kunkel S. L. Role of tumor necrosis factor-alpha in lipopolysaccharide-induced pathologic alterations. Am J Pathol. 1990 Jan;136(1):49–60. [PMC free article] [PubMed] [Google Scholar]
  23. Stokes D. C., Shenep J. L., Fishman M., Hildner W. K., Bysani G. K., Rufus K. Polymyxin B prevents lipopolysaccharide-induced release of tumor necrosis factor-alpha from alveolar macrophages. J Infect Dis. 1989 Jul;160(1):52–57. doi: 10.1093/infdis/160.1.52. [DOI] [PubMed] [Google Scholar]
  24. Suzuki K., Ota H., Sasagawa S., Sakatani T., Fujikura T. Assay method for myeloperoxidase in human polymorphonuclear leukocytes. Anal Biochem. 1983 Jul 15;132(2):345–352. doi: 10.1016/0003-2697(83)90019-2. [DOI] [PubMed] [Google Scholar]
  25. Takada H., Kawabata Y., Tamura M., Matsushita K., Igarashi H., Ohkuni H., Todome Y., Uchiyama T., Kotani S. Cytokine induction by extracellular products of oral viridans group streptococci. Infect Immun. 1993 Dec;61(12):5252–5260. doi: 10.1128/iai.61.12.5252-5260.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ulevitch R. J., Tobias P. S. Recognition of endotoxin by cells leading to transmembrane signaling. Curr Opin Immunol. 1994 Feb;6(1):125–130. doi: 10.1016/0952-7915(94)90043-4. [DOI] [PubMed] [Google Scholar]
  27. Wakabayashi G., Gelfand J. A., Jung W. K., Connolly R. J., Burke J. F., Dinarello C. A. Staphylococcus epidermidis induces complement activation, tumor necrosis factor and interleukin-1, a shock-like state and tissue injury in rabbits without endotoxemia. Comparison to Escherichia coli. J Clin Invest. 1991 Jun;87(6):1925–1935. doi: 10.1172/JCI115218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Weidemann B., Brade H., Rietschel E. T., Dziarski R., Bazil V., Kusumoto S., Flad H. D., Ulmer A. J. Soluble peptidoglycan-induced monokine production can be blocked by anti-CD14 monoclonal antibodies and by lipid A partial structures. Infect Immun. 1994 Nov;62(11):4709–4715. doi: 10.1128/iai.62.11.4709-4715.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Weiland J. E., Davis W. B., Holter J. F., Mohammed J. R., Dorinsky P. M., Gadek J. E. Lung neutrophils in the adult respiratory distress syndrome. Clinical and pathophysiologic significance. Am Rev Respir Dis. 1986 Feb;133(2):218–225. doi: 10.1164/arrd.1986.133.2.218. [DOI] [PubMed] [Google Scholar]
  30. Welbourn C. R., Young Y. Endotoxin, septic shock and acute lung injury: neutrophils, macrophages and inflammatory mediators. Br J Surg. 1992 Oct;79(10):998–1003. doi: 10.1002/bjs.1800791006. [DOI] [PubMed] [Google Scholar]
  31. Wiles J. B., Cerra F. B., Siegel J. H., Border J. R. The systemic septic response: does the organism matter? Crit Care Med. 1980 Feb;8(2):55–60. doi: 10.1097/00003246-198002000-00001. [DOI] [PubMed] [Google Scholar]
  32. Woods G. L., Wood R. P., Shaw B. W., Jr Aspergillus endocarditis in patients without prior cardiovascular surgery: report of a case in a liver transplant recipient and review. Rev Infect Dis. 1989 Mar-Apr;11(2):263–272. doi: 10.1093/clinids/11.2.263. [DOI] [PubMed] [Google Scholar]
  33. Zanetti G., Heumann D., Gérain J., Kohler J., Abbet P., Barras C., Lucas R., Glauser M. P., Baumgartner J. D. Cytokine production after intravenous or peritoneal gram-negative bacterial challenge in mice. Comparative protective efficacy of antibodies to tumor necrosis factor-alpha and to lipopolysaccharide. J Immunol. 1992 Mar 15;148(6):1890–1897. [PubMed] [Google Scholar]

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