Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1996 May;64(5):1879–1883. doi: 10.1128/iai.64.5.1879-1883.1996

Serum antibody responses of cattle following experimental infection with Escherichia coli O157:H7.

R P Johnson 1, W C Cray Jr 1, S T Johnson 1
PMCID: PMC174011  PMID: 8613410

Abstract

Oral inoculation of calves and steers with 10(10) CFU of Escherichia coli O157:H7 induced prompt and sustained increases in serum antibodies to O157 lipopolysaccharide. Neutralizing antibodies to verotoxin 1 also increased rapidly in most steers but more gradually in calves. None of the animals developed neutralizing antibodies to verotoxin 2. These serological responses were not correlated with elimination of infection in calves or steers or protection of calves against reinfection with the same strain.

Full Text

The Full Text of this article is available as a PDF (214.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Borczyk A. A., Karmali M. A., Lior H., Duncan L. M. Bovine reservoir for verotoxin-producing Escherichia coli O157:H7. Lancet. 1987 Jan 10;1(8524):98–98. doi: 10.1016/s0140-6736(87)91928-3. [DOI] [PubMed] [Google Scholar]
  2. Chapman P. A., Wright D. J., Norman P. Verotoxin-producing Escherichia coli infections in Sheffield: cattle as a possible source. Epidemiol Infect. 1989 Jun;102(3):439–445. doi: 10.1017/s0950268800030156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chart H., Scotland S. M., Rowe B. Serum antibodies to Escherichia coli serotype O157:H7 in patients with hemolytic uremic syndrome. J Clin Microbiol. 1989 Feb;27(2):285–290. doi: 10.1128/jcm.27.2.285-290.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark C. G., Johnson S., Johnson R. P. Further characterisation of a monoclonal antibody reactive with Escherichia coli O157:H7. J Med Microbiol. 1995 Oct;43(4):262–269. doi: 10.1099/00222615-43-4-262. [DOI] [PubMed] [Google Scholar]
  5. Corbel M. J., Stuart F. A., Brewer R. A. Observations on serological cross-reactions between smooth Brucella species and organisms of other genera. Dev Biol Stand. 1984;56:341–348. [PubMed] [Google Scholar]
  6. Cray W. C., Jr, Moon H. W. Experimental infection of calves and adult cattle with Escherichia coli O157:H7. Appl Environ Microbiol. 1995 Apr;61(4):1586–1590. doi: 10.1128/aem.61.4.1586-1590.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DEUTSCH H. F., MORTON J. I. Dissociation of human serum macroglobulins. Science. 1957 Mar 29;125(3248):600–601. doi: 10.1126/science.125.3248.600. [DOI] [PubMed] [Google Scholar]
  8. Dodds K. L., Perry M. B., McDonald I. J. Electrophoretic and immunochemical study of the lipopolysaccharides produced by chemostat-grown Escherichia coli O157. J Gen Microbiol. 1987 Sep;133(9):2679–2687. doi: 10.1099/00221287-133-9-2679. [DOI] [PubMed] [Google Scholar]
  9. Gannon V. P., Gyles C. L., Friendship R. W. Characteristics of verotoxigenic Escherichia coli from pigs. Can J Vet Res. 1988 Jul;52(3):331–337. [PMC free article] [PubMed] [Google Scholar]
  10. Greatorex J. S., Thorne G. M. Humoral immune responses to Shiga-like toxins and Escherichia coli O157 lipopolysaccharide in hemolytic-uremic syndrome patients and healthy subjects. J Clin Microbiol. 1994 May;32(5):1172–1178. doi: 10.1128/jcm.32.5.1172-1178.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Griffin P. M., Tauxe R. V. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol Rev. 1991;13:60–98. doi: 10.1093/oxfordjournals.epirev.a036079. [DOI] [PubMed] [Google Scholar]
  12. Hancock D. D., Besser T. E., Kinsel M. L., Tarr P. I., Rice D. H., Paros M. G. The prevalence of Escherichia coli O157.H7 in dairy and beef cattle in Washington State. Epidemiol Infect. 1994 Oct;113(2):199–207. doi: 10.1017/s0950268800051633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jerse A. E., Yu J., Tall B. D., Kaper J. B. A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Natl Acad Sci U S A. 1990 Oct;87(20):7839–7843. doi: 10.1073/pnas.87.20.7839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Johnson R. P., Boag L., Anderson S., Holtslander R., Rahn K., Clarke R. C., Renwick S. A., Alves D., Wilson J. B., Spika J. Brucella abortus serology in cattle naturally infected with Escherichia coli O157.H7. Vet Rec. 1994 Oct 15;135(16):382–383. doi: 10.1136/vr.135.16.382. [DOI] [PubMed] [Google Scholar]
  15. Karmali M. A. Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev. 1989 Jan;2(1):15–38. doi: 10.1128/cmr.2.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Karmali M. A., Petric M., Lim C., Fleming P. C., Arbus G. S., Lior H. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli. J Infect Dis. 1985 May;151(5):775–782. doi: 10.1093/infdis/151.5.775. [DOI] [PubMed] [Google Scholar]
  17. Konowalchuk J., Speirs J. I., Stavric S. Vero response to a cytotoxin of Escherichia coli. Infect Immun. 1977 Dec;18(3):775–779. doi: 10.1128/iai.18.3.775-779.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  19. Lance J. W., Bassil G. T. Familial intractable hiccup relieved by baclofen. Lancet. 1989 Jul 29;2(8657):276–277. doi: 10.1016/s0140-6736(89)90460-1. [DOI] [PubMed] [Google Scholar]
  20. Levine M. M., Xu J. G., Kaper J. B., Lior H., Prado V., Tall B., Nataro J., Karch H., Wachsmuth K. A DNA probe to identify enterohemorrhagic Escherichia coli of O157:H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome. J Infect Dis. 1987 Jul;156(1):175–182. doi: 10.1093/infdis/156.1.175. [DOI] [PubMed] [Google Scholar]
  21. Montenegro M. A., Bülte M., Trumpf T., Aleksić S., Reuter G., Bulling E., Helmuth R. Detection and characterization of fecal verotoxin-producing Escherichia coli from healthy cattle. J Clin Microbiol. 1990 Jun;28(6):1417–1421. doi: 10.1128/jcm.28.6.1417-1421.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Notenboom R. H., Borczyk A., Karmali M. A., Duncan L. M. Clinical relevance of a serological cross-reaction between Escherichia coli O157 and Brucella abortus. Lancet. 1987 Sep 26;2(8561):745–745. doi: 10.1016/s0140-6736(87)91106-8. [DOI] [PubMed] [Google Scholar]
  23. O'Brien A. D., Holmes R. K. Shiga and Shiga-like toxins. Microbiol Rev. 1987 Jun;51(2):206–220. doi: 10.1128/mr.51.2.206-220.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Obrig T. G., Del Vecchio P. J., Brown J. E., Moran T. P., Rowland B. M., Judge T. K., Rothman S. W. Direct cytotoxic action of Shiga toxin on human vascular endothelial cells. Infect Immun. 1988 Sep;56(9):2373–2378. doi: 10.1128/iai.56.9.2373-2378.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Perry M. B., MacLean L., Griffith D. W. Structure of the O-chain polysaccharide of the phenol-phase soluble lipopolysaccharide of Escherichia coli 0:157:H7. Biochem Cell Biol. 1986 Jan;64(1):21–28. doi: 10.1139/o86-004. [DOI] [PubMed] [Google Scholar]
  26. Pirro F., Wieler L. H., Failing K., Bauerfeind R., Baljer G. Neutralizing antibodies against Shiga-like toxins from Escherichia coli in colostra and sera of cattle. Vet Microbiol. 1995 Feb;43(2-3):131–141. doi: 10.1016/0378-1135(94)00089-f. [DOI] [PubMed] [Google Scholar]
  27. Riley L. W., Remis R. S., Helgerson S. D., McGee H. B., Wells J. G., Davis B. R., Hebert R. J., Olcott E. S., Johnson L. M., Hargrett N. T. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N Engl J Med. 1983 Mar 24;308(12):681–685. doi: 10.1056/NEJM198303243081203. [DOI] [PubMed] [Google Scholar]
  28. Siddons C. A., Chapman P. A. Detection of serum and faecal antibodies in haemorrhagic colitis caused by Escherichia coli O157. J Med Microbiol. 1993 Dec;39(6):408–415. doi: 10.1099/00222615-39-6-408. [DOI] [PubMed] [Google Scholar]
  29. Stemshorn B. W. Bovine brucellosis - diagnosis and eradication. Can Vet J. 1985 Jan;26(1):35–39. [PMC free article] [PubMed] [Google Scholar]
  30. Strockbine N. A., Marques L. R., Newland J. W., Smith H. W., Holmes R. K., O'Brien A. D. Two toxin-converting phages from Escherichia coli O157:H7 strain 933 encode antigenically distinct toxins with similar biologic activities. Infect Immun. 1986 Jul;53(1):135–140. doi: 10.1128/iai.53.1.135-140.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Stuart F. A., Corbel M. J. Identification of a serological cross-reaction between Brucella abortus and Escherichia coli 0:157. Vet Rec. 1982 Feb 27;110(9):202–203. doi: 10.1136/vr.110.9.202-a. [DOI] [PubMed] [Google Scholar]
  32. Tarr P. I. Escherichia coli O157:H7: clinical, diagnostic, and epidemiological aspects of human infection. Clin Infect Dis. 1995 Jan;20(1):1–10. doi: 10.1093/clinids/20.1.1. [DOI] [PubMed] [Google Scholar]
  33. Thomas A., Chart H., Cheasty T., Smith H. R., Frost J. A., Rowe B. Vero cytotoxin-producing Escherichia coli, particularly serogroup O 157, associated with human infections in the United Kingdom: 1989-91. Epidemiol Infect. 1993 Jun;110(3):591–600. doi: 10.1017/s0950268800051013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Thomas L. V., Gross R. J., Cheasty T., Shipp C. R., Rowe B. Antigenic relationships among type strains of Yersinia enterocolitica and those of Escherichia coli, Salmonella spp., and Shigella spp. J Clin Microbiol. 1983 Jan;17(1):109–111. doi: 10.1128/jcm.17.1.109-111.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Tokhi A. M., Peiris J. S., Scotland S. M., Willshaw G. A., Smith H. R., Cheasty T. A longitudinal study of Vero cytotoxin producing Escherichia coli in cattle calves in Sri Lanka. Epidemiol Infect. 1993 Apr;110(2):197–208. doi: 10.1017/s0950268800068114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Wells J. G., Davis B. R., Wachsmuth I. K., Riley L. W., Remis R. S., Sokolow R., Morris G. K. Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. J Clin Microbiol. 1983 Sep;18(3):512–520. doi: 10.1128/jcm.18.3.512-520.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Wells J. G., Shipman L. D., Greene K. D., Sowers E. G., Green J. H., Cameron D. N., Downes F. P., Martin M. L., Griffin P. M., Ostroff S. M. Isolation of Escherichia coli serotype O157:H7 and other Shiga-like-toxin-producing E. coli from dairy cattle. J Clin Microbiol. 1991 May;29(5):985–989. doi: 10.1128/jcm.29.5.985-989.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Willshaw G. A., Scotland S. M., Smith H. R., Cheasty T., Thomas A., Rowe B. Hybridization of strains of Escherichia coli O157 with probes derived from the eaeA gene of enteropathogenic E. coli and the eaeA homolog from a Vero cytotoxin-producing strain of E. coli O157. J Clin Microbiol. 1994 Apr;32(4):897–902. doi: 10.1128/jcm.32.4.897-902.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Wilson J. B., McEwen S. A., Clarke R. C., Leslie K. E., Wilson R. A., Waltner-Toews D., Gyles C. L. Distribution and characteristics of verocytotoxigenic Escherichia coli isolated from Ontario dairy cattle. Epidemiol Infect. 1992 Jun;108(3):423–439. doi: 10.1017/s0950268800049931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Zhao T., Doyle M. P., Shere J., Garber L. Prevalence of enterohemorrhagic Escherichia coli O157:H7 in a survey of dairy herds. Appl Environ Microbiol. 1995 Apr;61(4):1290–1293. doi: 10.1128/aem.61.4.1290-1293.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES