Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1996 Jul;64(7):2799–2803. doi: 10.1128/iai.64.7.2799-2803.1996

Number of specific antibody-secreting cells in the peripheral blood among children with mycoplasma pneumonia.

M Iseki 1, T Takahashi 1, K Kimura 1, R Yamashita 1, T Sasaki 1
PMCID: PMC174142  PMID: 8698511

Abstract

Mycoplasma pneumoniae-specific antibody-secreting cells (ASCs) in the peripheral blood were enumerated with an enzyme-linked immunospot assay in 12 children with mycoplasma pneumonia. Those cells were detected in the acute phases and declined in number in the convalescent stage. The maximum numbers of M. pneumoniae-specific ASCs ranged from 0 to 478 for immunoglobulin G (IgG), 13 to 1,992 for IgM, and 0 to 53 for IgA per 106 peripheral blood mononuclear cells, whereas the total numbers (i.e., including both specific and nonspecific) of immunoglobulin-secreting cells (IgSCs) were as high as 4,000 for both IgG and IgM and 1,000 for IgA per 106 peripheral blood mononuclear cells. Such a great increase in the numbers of total IgSCs in comparison with that in M. pneumoniae-specific ASCs suggests that the majority of the IgSC increase in the course of mycoplasmal infection was nonspecific to M. pneumoniae. The serum level of M. pneumoniae antibody measured by enzyme-linked immunosorbent assay remained high in the convalescent phase, while the number of specific ASCs decreased. Whereas this observation may be explained by declined degeneration or consumption of the antibody in the convalescent phase, it may be suggestive of the source of M. pneumoniae antibody other than ASCs in the peripheral blood.

Full Text

The Full Text of this article is available as a PDF (272.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison A. C., Nawata Y. Cytokines mediating the proliferation and differentiation of B-1 lymphocytes and their role in ontogeny and phylogeny. Ann N Y Acad Sci. 1992 May 4;651:200–219. doi: 10.1111/j.1749-6632.1992.tb24616.x. [DOI] [PubMed] [Google Scholar]
  2. Biberfeld G., Arneborn P., Forsgren M., von Stedingk L. V., Blomqvist S. Non-specific polyclonal antibody response induced by Mycoplasma pneumoniae. Yale J Biol Med. 1983 Sep-Dec;56(5-6):639–642. [PMC free article] [PubMed] [Google Scholar]
  3. Brieva J. A., Stevens R. H. Human in vivo antigen-induced lymphoblastoid B cells are capable of cyclical antibody production in vitro. J Immunol. 1984 Jul;133(1):147–153. [PubMed] [Google Scholar]
  4. Heilmann C., Pedersen F. K. Quantitation of blood lymphocytes secreting antibodies to pneumococcal polysaccharides after in vivo antigenic stimulation. Scand J Immunol. 1986 Feb;23(2):189–194. doi: 10.1111/j.1365-3083.1986.tb01957.x. [DOI] [PubMed] [Google Scholar]
  5. Iseki M., Ank B. J., Stiehm E. R. Peripheral immunoglobulin secreting cells in immunodeficiencies; effects of intravenous immune globulin. Ann Allergy. 1994 May;72(5):419–424. [PubMed] [Google Scholar]
  6. Jacobs E., Schöpperle K., Bredt W. Adherence inhibition assay: a specific serological test for detection of antibodies to Mycoplasma pneumoniae. Eur J Clin Microbiol. 1985 Apr;4(2):113–118. doi: 10.1007/BF02013574. [DOI] [PubMed] [Google Scholar]
  7. Lind K., Høier-Madsen M., Wiik A. Autoantibodies to the mitotic spindle apparatus in Mycoplasma pneumoniae disease. Infect Immun. 1988 Mar;56(3):714–715. doi: 10.1128/iai.56.3.714-715.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lue C., Prince S. J., Fattom A., Schneerson R., Robbins J. B., Mestecky J. Antibody-secreting peripheral blood lymphocytes induced by immunization with a conjugate consisting of Streptococcus pneumoniae type 12F polysaccharide and diphtheria toxoid. Infect Immun. 1990 Aug;58(8):2547–2554. doi: 10.1128/iai.58.8.2547-2554.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lue C., Tarkowski A., Mestecky J. Systemic immunization with pneumococcal polysaccharide vaccine induces a predominant IgA2 response of peripheral blood lymphocytes and increases of both serum and secretory anti-pneumococcal antibodies. J Immunol. 1988 Jun 1;140(11):3793–3800. [PubMed] [Google Scholar]
  10. Mor G., Klinman D. M., Shapiro S., Hagiwara E., Sedegah M., Norman J. A., Hoffman S. L., Steinberg A. D. Complexity of the cytokine and antibody response elicited by immunizing mice with Plasmodium yoelii circumsporozoite protein plasmid DNA. J Immunol. 1995 Aug 15;155(4):2039–2046. [PubMed] [Google Scholar]
  11. Sasaki T., Bonissol C., Stoiljkovic B., Ito K. Demonstration of cross-reactive antibodies to mycoplasmas in human sera by ELISA and immunoblotting. Microbiol Immunol. 1987;31(7):639–648. doi: 10.1111/j.1348-0421.1987.tb03124.x. [DOI] [PubMed] [Google Scholar]
  12. Sasaki T., Sasaki Y., Kita M., Suzuki K., Watanabe H., Honda M. Evidence that Lo's mycoplasma (Mycoplasma fermentans incognitus) is not a unique strain among Mycoplasma fermentans strains. J Clin Microbiol. 1992 Sep;30(9):2435–2440. doi: 10.1128/jcm.30.9.2435-2440.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Saxon A., Mitsuyasu R., Stevens R., Champlin R. E., Kimata H., Gale R. P. Designed transfer of specific immune responses with bone marrow transplantation. J Clin Invest. 1986 Oct;78(4):959–967. doi: 10.1172/JCI112686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Simecka J. W., Patel P., Davis J. K., Ross S. E., Otwell P., Cassell G. H. Specific and nonspecific antibody responses in different segments of the respiratory tract in rats infected with Mycoplasma pulmonis. Infect Immun. 1991 Oct;59(10):3715–3721. doi: 10.1128/iai.59.10.3715-3721.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Simecka J. W., Ross S. E., Cassell G. H., Davis J. K. Interactions of mycoplasmas with B cells: antibody production and nonspecific effects. Clin Infect Dis. 1993 Aug;17 (Suppl 1):S176–S182. doi: 10.1093/clinids/17.supplement_1.s176. [DOI] [PubMed] [Google Scholar]
  16. Stevens R. H., Macy E., Morrow C., Saxon A. Characterization of a circulating subpopulation of spontaneous antitetanus toxoid antibody producing B cells following in vivo booster immunization. J Immunol. 1979 Jun;122(6):2498–2504. [PubMed] [Google Scholar]
  17. Stoll B. J., Lee F. K., Hale E., Schwartz D., Holmes R., Ashby R., Czerkinsky C., Nahmias A. J. Immunoglobulin secretion by the normal and the infected newborn infant. J Pediatr. 1993 May;122(5 Pt 1):780–786. doi: 10.1016/s0022-3476(06)80026-0. [DOI] [PubMed] [Google Scholar]
  18. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J Hyg (Lond) 1966 Mar;64(1):91–104. doi: 10.1017/s0022172400040377. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES