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. 1996 Jul;64(7):2824–2828. doi: 10.1128/iai.64.7.2824-2828.1996

Structural and immunological characterization of Burkholderia pseudomallei O-polysaccharide-flagellin protein conjugates.

P J Brett 1, D E Woods 1
PMCID: PMC174148  PMID: 8698517

Abstract

The O-polysaccharide moiety of Burkholderia pseudomallei 319a lipopolysaccharide was covalently linked to flagellin protein isolated from the same strain. A glycoconjugate incorporating adipic acid dihydrazide as a spacer molecule elicited high-titer immunoglobulin G responses to both the protein and carbohydrate components of the construct. This immunoglobulin G was capable of protecting diabetic rats from challenge with a heterologous B. pseudomallei strain.

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Selected References

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