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. 1996 Jul;64(7):2873–2876. doi: 10.1128/iai.64.7.2873-2876.1996

Pulmonary damage by Vibrio vulnificus cytolysin.

J W Park 1, S N Ma 1, E S Song 1, C H Song 1, M R Chae 1, B H Park 1, R W Rho 1, S D Park 1, H R Kim 1
PMCID: PMC174159  PMID: 8698528

Abstract

Vibrio vulnificus is an estuarine bacterium that causes septicemia and serious wound infection. Cytolysin produced by V. vulnificus has been incriminated as one of the important virulence determinants of bacterial infection. Cytolysin (8 hemolytic units) given intravenously to mice via their tail veins caused severe hemoconcentration and lethality. Cytolysin treatment greatly increased pulmonary wet weight and vascular permeability as measured by (125)I-labeled albumin leakage without affecting those factors of other organs significantly. Blood neutrophils were markedly decreased in number after cytolysin injection, with a concomitant increase in the level of pulmonary myeloperoxidase activity, indicating that cytolysin-induced neutropenia might be due to pulmonary sequestration of neutrophils. By microscopic examination, severe perivascular edema and neutrophil infiltration were evident in lung tissues. These results suggest that increased vascular permeability and neutrophil sequestration in the lungs are important factors in lethal activity by cytolysin.

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Selected References

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  1. Albelda S. M., Karnovsky M. J., Fishman A. P. Perspectives in endothelial cell biology. J Appl Physiol (1985) 1987 Apr;62(4):1345–1348. doi: 10.1152/jappl.1987.62.4.1345. [DOI] [PubMed] [Google Scholar]
  2. Anderson B. O., Brown J. M., Shanley P. F., Bensard D. D., Harken A. H. Marginating neutrophils are reversibly adherent to normal lung endothelium. Surgery. 1991 Jan;109(1):51–61. [PubMed] [Google Scholar]
  3. BERNHEIMER A. W., SCHWARTZ L. L. Isolation and composition of staphylococcal alpha toxin. J Gen Microbiol. 1963 Mar;30:455–468. doi: 10.1099/00221287-30-3-455. [DOI] [PubMed] [Google Scholar]
  4. Bhakdi S., Greulich S., Muhly M., Eberspächer B., Becker H., Thiele A., Hugo F. Potent leukocidal action of Escherichia coli hemolysin mediated by permeabilization of target cell membranes. J Exp Med. 1989 Mar 1;169(3):737–754. doi: 10.1084/jem.169.3.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bhakdi S., Muhly M., Mannhardt U., Hugo F., Klapettek K., Mueller-Eckhardt C., Roka L. Staphylococcal alpha toxin promotes blood coagulation via attack on human platelets. J Exp Med. 1988 Aug 1;168(2):527–542. doi: 10.1084/jem.168.2.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bhakdi S., Tranum-Jensen J. Damage to cell membranes by pore-forming bacterial cytolysins. Prog Allergy. 1988;40:1–43. [PubMed] [Google Scholar]
  7. Blake P. A., Merson M. H., Weaver R. E., Hollis D. G., Heublein P. C. Disease caused by a marine Vibrio. Clinical characteristics and epidemiology. N Engl J Med. 1979 Jan 4;300(1):1–5. doi: 10.1056/NEJM197901043000101. [DOI] [PubMed] [Google Scholar]
  8. Fraker P. J., Speck J. C., Jr Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978 Feb 28;80(4):849–857. doi: 10.1016/0006-291x(78)91322-0. [DOI] [PubMed] [Google Scholar]
  9. Goldblum S. E., Wu K. M., Jay M. Lung myeloperoxidase as a measure of pulmonary leukostasis in rabbits. J Appl Physiol (1985) 1985 Dec;59(6):1978–1985. doi: 10.1152/jappl.1985.59.6.1978. [DOI] [PubMed] [Google Scholar]
  10. Goris R. J., te Boekhorst T. P., Nuytinck J. K., Gimbrère J. S. Multiple-organ failure. Generalized autodestructive inflammation? Arch Surg. 1985 Oct;120(10):1109–1115. doi: 10.1001/archsurg.1985.01390340007001. [DOI] [PubMed] [Google Scholar]
  11. Gray L. D., Kreger A. S. Detection of Vibrio vulnificus cytolysin in V. vulnificus-infected mice. Toxicon. 1989;27(4):439–464. doi: 10.1016/0041-0101(89)90208-0. [DOI] [PubMed] [Google Scholar]
  12. Gray L. D., Kreger A. S. Mouse skin damage caused by cytolysin from Vibrio vulnificus and by V. vulnificus infection. J Infect Dis. 1987 Feb;155(2):236–241. doi: 10.1093/infdis/155.2.236. [DOI] [PubMed] [Google Scholar]
  13. Gray L. D., Kreger A. S. Purification and characterization of an extracellular cytolysin produced by Vibrio vulnificus. Infect Immun. 1985 Apr;48(1):62–72. doi: 10.1128/iai.48.1.62-72.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kim H. R., Rho H. W., Jeong M. H., Park J. W., Kim J. S., Park B. H., Kim U. H., Park S. D. Hemolytic mechanism of cytolysin produced from V. vulnificus. Life Sci. 1993;53(7):571–577. doi: 10.1016/0024-3205(93)90714-e. [DOI] [PubMed] [Google Scholar]
  15. Kinkade J. M., Jr, Pember S. O., Barnes K. C., Shapira R., Spitznagel J. K., Martin L. E. Differential distribution of distinct forms of myeloperoxidase in different azurophilic granule subpopulations from human neutrophils. Biochem Biophys Res Commun. 1983 Jul 18;114(1):296–303. doi: 10.1016/0006-291x(83)91627-3. [DOI] [PubMed] [Google Scholar]
  16. Kreger A. S., Kothary M. H., Gray L. D. Cytolytic toxins of Vibrio vulnificus and Vibrio damsela. Methods Enzymol. 1988;165:176–189. doi: 10.1016/s0076-6879(88)65028-2. [DOI] [PubMed] [Google Scholar]
  17. Kreger A., Lockwood D. Detection of extracellular toxin(s) produced by Vibrio vulnificus. Infect Immun. 1981 Aug;33(2):583–590. doi: 10.1128/iai.33.2.583-590.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McDonald R. J., Berger E. M., Repine J. E. Neutrophil-derived oxygen metabolites stimulate thromboxane release, pulmonary artery pressure increases, and weight gains in isolated perfused rat lungs. Am Rev Respir Dis. 1987 Apr;135(4):957–959. doi: 10.1164/arrd.1987.135.4.957. [DOI] [PubMed] [Google Scholar]
  19. McMenamy R. H., Birkhahn R., Oswald G., Reed R., Rumph C., Vaidyanath N., Yu L., Cerra F. B., Sorkness R., Border J. R. Multiple systems organ failure: I. The basal state. J Trauma. 1981 Feb;21(2):99–114. doi: 10.1097/00005373-198102000-00003. [DOI] [PubMed] [Google Scholar]
  20. Meyrick B., Brigham K. L. Acute effects of Escherichia coli endotoxin on the pulmonary microcirculation of anesthetized sheep structure:function relationships. Lab Invest. 1983 Apr;48(4):458–470. [PubMed] [Google Scholar]
  21. Oliver J. D., Wear J. E., Thomas M. B., Warner M., Linder K. Production of extracellular enzymes and cytotoxicity by Vibrio vulnificus. Diagn Microbiol Infect Dis. 1986 Jul;5(2):99–111. doi: 10.1016/0732-8893(86)90112-4. [DOI] [PubMed] [Google Scholar]
  22. Park J. W., Jahng T. A., Rho H. W., Park B. H., Kim N. H., Kim H. R. Inhibitory mechanism of Ca2+ on the hemolysis caused by Vibrio vulnificus cytolysin. Biochim Biophys Acta. 1994 Aug 24;1194(1):166–170. doi: 10.1016/0005-2736(94)90216-x. [DOI] [PubMed] [Google Scholar]
  23. Park S. D., Shon H. S., Joh N. J. Vibrio vulnificus septicemia in Korea: clinical and epidemiologic findings in seventy patients. J Am Acad Dermatol. 1991 Mar;24(3):397–403. doi: 10.1016/0190-9622(91)70059-b. [DOI] [PubMed] [Google Scholar]
  24. Perlo S., Jalowayski A. A., Durand C. M., West J. B. Distribution of red and white blood cells in alveolar walls. J Appl Physiol. 1975 Jan;38(1):117–124. doi: 10.1152/jappl.1975.38.1.117. [DOI] [PubMed] [Google Scholar]
  25. SCHULTZ J., KAMINKER K. Myeloperoxidase of the leucocyte of normal human blood. I. Content and localization. Arch Biochem Biophys. 1962 Mar;96:465–467. doi: 10.1016/0003-9861(62)90321-1. [DOI] [PubMed] [Google Scholar]
  26. Seekamp A., Mulligan M. S., Till G. O., Smith C. W., Miyasaka M., Tamatani T., Todd R. F., 3rd, Ward P. A. Role of beta 2 integrins and ICAM-1 in lung injury following ischemia-reperfusion of rat hind limbs. Am J Pathol. 1993 Aug;143(2):464–472. [PMC free article] [PubMed] [Google Scholar]
  27. Smedly L. A., Tonnesen M. G., Sandhaus R. A., Haslett C., Guthrie L. A., Johnston R. B., Jr, Henson P. M., Worthen G. S. Neutrophil-mediated injury to endothelial cells. Enhancement by endotoxin and essential role of neutrophil elastase. J Clin Invest. 1986 Apr;77(4):1233–1243. doi: 10.1172/JCI112426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Till G. O., Johnson K. J., Kunkel R., Ward P. A. Intravascular activation of complement and acute lung injury. Dependency on neutrophils and toxic oxygen metabolites. J Clin Invest. 1982 May;69(5):1126–1135. doi: 10.1172/JCI110548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Weiss S. J. Tissue destruction by neutrophils. N Engl J Med. 1989 Feb 9;320(6):365–376. doi: 10.1056/NEJM198902093200606. [DOI] [PubMed] [Google Scholar]
  30. Worthen G. S., Haslett C., Rees A. J., Gumbay R. S., Henson J. E., Henson P. M. Neutrophil-mediated pulmonary vascular injury. Synergistic effect of trace amounts of lipopolysaccharide and neutrophil stimuli on vascular permeability and neutrophil sequestration in the lung. Am Rev Respir Dis. 1987 Jul;136(1):19–28. doi: 10.1164/ajrccm/136.1.19. [DOI] [PubMed] [Google Scholar]
  31. Wright A. C., Morris J. G., Jr The extracellular cytolysin of Vibrio vulnificus: inactivation and relationship to virulence in mice. Infect Immun. 1991 Jan;59(1):192–197. doi: 10.1128/iai.59.1.192-197.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

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