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. 1996 Jul;64(7):2881–2884. doi: 10.1128/iai.64.7.2881-2884.1996

The Hha protein as a modulator of expression of virulence factors in Escherichia coli.

M Mouriño 1, C Madrid 1, C Balsalobre 1, A Prenafeta 1, F Muñoa 1, J Blanco 1, M Blanco 1, J E Blanco 1, A Juarez 1
PMCID: PMC174161  PMID: 8698530

Abstract

We constructed hha derivatives from both a clinical uropathogenic Escherichia coli isolate (strain FVL4) and a wild E. coli strain causing bovine diarrhea (strain CCB21) and analyzed the effect of the hha allele on the expression of the different virulence factors exhibited by these strains. Expression of hemolysin and of the Vir antigen was altered in hha mutants. Whereas production of hemolysin by strain FVL4 was repressed both at a low temperature and at high osmolarity, the hha allele accounted for a significant increase of hemolysin production under these conditions. Also, the low temperature-sensitive expression of the Vir adhesin was modified in hha mutants, which were able to express this adhesin at a low temperature. Expression of other virulence factors, such as cytotoxic necrotizing factor type 1 and 2 toxins, remained unmodified in hha derivatives of strains FVL4 and CCB21.

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Selected References

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