Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1996 Aug;64(8):3394–3396. doi: 10.1128/iai.64.8.3394-3396.1996

Sequence of the toxic shock syndrome toxin gene (tstH) borne by strains of Staphylococcus aureus isolated from patients with Kawasaki syndrome.

R L Deresiewicz 1, J Flaxenburg 1, K Leng 1, D L Kasper 1
PMCID: PMC174235  PMID: 8757881

Abstract

To explore whether a novel staphylococcal clone or structural variant of toxic shock syndrome toxin 1 is associated with Kawasaki syndrome, six toxigenic strains of Staphylococcus aureus from Kawasaki syndrome patients were studied. The strains were divisible into two groups based on phenotypic and genotypic characteristics and are therefore unequivocally not clonal. Portions of the tstH genes of each strain were sequenced. Three were sequenced in their entirety, while the remainder were sequenced from codon 66 to codon 137 of the mature protein only. Two of the former group differed slightly in the sequences of their signal peptides relative to the sequence published for the tstH signal peptide. Those differences did not affect toxin processing or secretion. The sequenced portions of the regions encoding mature toxic shock syndrome toxin 1 were identical in all six strains and corresponded exactly to the published sequence of tstH. No evidence was found for the existence of a structural variant of tstH uniquely associated with Kawasaki syndrome.

Full Text

The Full Text of this article is available as a PDF (202.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe J., Kotzin B. L., Jujo K., Melish M. E., Glode M. P., Kohsaka T., Leung D. Y. Selective expansion of T cells expressing T-cell receptor variable regions V beta 2 and V beta 8 in Kawasaki disease. Proc Natl Acad Sci U S A. 1992 May 1;89(9):4066–4070. doi: 10.1073/pnas.89.9.4066. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blomster-Hautamaa D. A., Kreiswirth B. N., Kornblum J. S., Novick R. P., Schlievert P. M. The nucleotide and partial amino acid sequence of toxic shock syndrome toxin-1. J Biol Chem. 1986 Nov 25;261(33):15783–15786. [PubMed] [Google Scholar]
  3. Chu M. C., Kreiswirth B. N., Pattee P. A., Novick R. P., Melish M. E., James J. F. Association of toxic shock toxin-1 determinant with a heterologous insertion at multiple loci in the Staphylococcus aureus chromosome. Infect Immun. 1988 Oct;56(10):2702–2708. doi: 10.1128/iai.56.10.2702-2708.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Deresiewicz R. L., Woo J., Chan M., Finberg R. W., Kasper D. L. Mutations affecting the activity of toxic shock syndrome toxin-1. Biochemistry. 1994 Nov 1;33(43):12844–12851. doi: 10.1021/bi00209a016. [DOI] [PubMed] [Google Scholar]
  5. Kreiswirth B. N., Novick R. P., Schlievert P. M., Bergdoll M. Genetic studies on Staphylococcal strains from patients with toxic shock syndrome. Ann Intern Med. 1982 Jun;96(6 Pt 2):974–977. doi: 10.7326/0003-4819-96-6-974. [DOI] [PubMed] [Google Scholar]
  6. Kreiswirth B. N., Projan S. J., Schlievert P. M., Novick R. P. Toxic shock syndrome toxin 1 is encoded by a variable genetic element. Rev Infect Dis. 1989 Jan-Feb;11 (Suppl 1):S83–S89. doi: 10.1093/clinids/11.supplement_1.s83. [DOI] [PubMed] [Google Scholar]
  7. Lee P. K., Kreiswirth B. N., Deringer J. R., Projan S. J., Eisner W., Smith B. L., Carlson E., Novick R. P., Schlievert P. M. Nucleotide sequences and biologic properties of toxic shock syndrome toxin 1 from ovine- and bovine-associated Staphylococcus aureus. J Infect Dis. 1992 Jun;165(6):1056–1063. doi: 10.1093/infdis/165.6.1056. [DOI] [PubMed] [Google Scholar]
  8. Leung D. Y., Meissner H. C., Fulton D. R., Murray D. L., Kotzin B. L., Schlievert P. M. Toxic shock syndrome toxin-secreting Staphylococcus aureus in Kawasaki syndrome. Lancet. 1993 Dec 4;342(8884):1385–1388. doi: 10.1016/0140-6736(93)92752-f. [DOI] [PubMed] [Google Scholar]
  9. Pietra B. A., De Inocencio J., Giannini E. H., Hirsch R. TCR V beta family repertoire and T cell activation markers in Kawasaki disease. J Immunol. 1994 Aug 15;153(4):1881–1888. [PubMed] [Google Scholar]
  10. Rauch A. M., Hurwitz E. S. Centers for Disease Control (CDC) case definition for Kawasaki syndrome. Pediatr Infect Dis. 1985 Nov-Dec;4(6):702–703. doi: 10.1097/00006454-198511000-00029. [DOI] [PubMed] [Google Scholar]
  11. Reingold A. L., Hargrett N. T., Shands K. N., Dan B. B., Schmid G. P., Strickland B. Y., Broome C. V. Toxic shock syndrome surveillance in the United States, 1980 to 1981. Ann Intern Med. 1982 Jun;96(6 Pt 2):875–880. doi: 10.7326/0003-4819-96-6-875. [DOI] [PubMed] [Google Scholar]
  12. Rowley A. H., Gonzalez-Crussi F., Shulman S. T. Kawasaki syndrome. Rev Infect Dis. 1988 Jan-Feb;10(1):1–15. doi: 10.1093/clinids/10.1.1. [DOI] [PubMed] [Google Scholar]
  13. Schlievert P. M., Blomster D. A. Production of staphylococcal pyrogenic exotoxin type C: influence of physical and chemical factors. J Infect Dis. 1983 Feb;147(2):236–242. doi: 10.1093/infdis/147.2.236. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES