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. 1996 Sep;64(9):3934–3936. doi: 10.1128/iai.64.9.3934-3936.1996

Urease activity does not contribute dramatically to persistence of Mycobacterium bovis bacillus Calmette-Guérin.

J M Reyrat 1, G Lopez-Ramirez 1, C Ofredo 1, B Gicquel 1, N Winter 1
PMCID: PMC174318  PMID: 8751954

Abstract

Multiplication of BCGure-, an isogenic urease-negative mutant of Mycobacterium bovis BCG constructed by allelic exchange (J. M. Reyrat, F. X. Berthet, and B. Gicquel, Proc. Natl. Acad. Sci. USA 92:8768-8772, 1995), was examined in human macrophages and mice. Although ureolytic activity was not essential to BCGure-growth, a slight decrease in the multiplication and persistence of the mutated strain compared with wild-type BCG was observed in lungs of infected mice.

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Selected References

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