Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1996 Sep;64(9):3946–3949. doi: 10.1128/iai.64.9.3946-3949.1996

Treatment with the antigranulocyte monoclonal antibody RB6-8C5 impairs resistance of mice to gastrointestinal infection with Listeria monocytogenes.

C J Czuprynski 1, C Theisen 1, J F Brown 1
PMCID: PMC174321  PMID: 8751957

Abstract

Treatment with the antigranulocyte monoclonal antibody (MAb) RB6-8C5 increased the severity of infection in mice intragastrically inoculated with Listeria monocytogenes. Most MAb RB6-8C5-treated mice died when inoculated intragastrically with as few as 4 x 10(4) L. monocytogenes bacteria, whereas most control mice survived intragastric inoculation with 4 x 10(8) L. monocytogenes bacteria. The increased severity of infection in MAb RB6-8C5-treated mice appeared to result from listerial multiplication in the spleen and liver rather than from local proliferation in the intestinal tract or mesenteric lymph nodes.

Full Text

The Full Text of this article is available as a PDF (173.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appelberg R., Castro A. G., Silva M. T. Neutrophils as effector cells of T-cell-mediated, acquired immunity in murine listeriosis. Immunology. 1994 Oct;83(2):302–307. [PMC free article] [PubMed] [Google Scholar]
  2. Boury N. M., Czuprynski C. J. Listeria monocytogenes infection increases neutrophil adhesion and damage to a murine hepatocyte cell line in vitro. Immunol Lett. 1995 May;46(1-2):111–116. doi: 10.1016/0165-2478(95)00027-3. [DOI] [PubMed] [Google Scholar]
  3. Czuprynski C. J., Brown J. F., Maroushek N., Wagner R. D., Steinberg H. Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection. J Immunol. 1994 Feb 15;152(4):1836–1846. [PubMed] [Google Scholar]
  4. Czuprynski C. J., Brown J. F., Wagner R. D., Steinberg H. Administration of antigranulocyte monoclonal antibody RB6-8C5 prevents expression of acquired resistance to Listeria monocytogenes infection in previously immunized mice. Infect Immun. 1994 Nov;62(11):5161–5163. doi: 10.1128/iai.62.11.5161-5163.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Farber J. M., Peterkin P. I. Listeria monocytogenes, a food-borne pathogen. Microbiol Rev. 1991 Sep;55(3):476–511. doi: 10.1128/mr.55.3.476-511.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fleming T. J., Fleming M. L., Malek T. R. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993 Sep 1;151(5):2399–2408. [PubMed] [Google Scholar]
  7. Haponsaph R., Czuprynski C. J. Inhibition of the multiplication of Listeria monocytogenes in a murine hepatocyte cell line (ATCC TIB73) by IFN-gamma and TNF-alpha. Microb Pathog. 1996 May;20(5):287–295. doi: 10.1006/mpat.1996.0027. [DOI] [PubMed] [Google Scholar]
  8. Hestdal K., Ruscetti F. W., Ihle J. N., Jacobsen S. E., Dubois C. M., Kopp W. C., Longo D. L., Keller J. R. Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J Immunol. 1991 Jul 1;147(1):22–28. [PubMed] [Google Scholar]
  9. Jensen J., Warner T., Balish E. Resistance of SCID mice to Candida albicans administered intravenously or colonizing the gut: role of polymorphonuclear leukocytes and macrophages. J Infect Dis. 1993 Apr;167(4):912–919. doi: 10.1093/infdis/167.4.912. [DOI] [PubMed] [Google Scholar]
  10. Kaufmann S. H. Immunity to intracellular bacteria. Annu Rev Immunol. 1993;11:129–163. doi: 10.1146/annurev.iy.11.040193.001021. [DOI] [PubMed] [Google Scholar]
  11. Lammerding A. M., Glass K. A., Gendron-Fitzpatrick A., Doyle M. P. Determination of virulence of different strains of Listeria monocytogenes and Listeria innocua by oral inoculation of pregnant mice. Appl Environ Microbiol. 1992 Dec;58(12):3991–4000. doi: 10.1128/aem.58.12.3991-4000.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lloyd A. R., Oppenheim J. J. Poly's lament: the neglected role of the polymorphonuclear neutrophil in the afferent limb of the immune response. Immunol Today. 1992 May;13(5):169–172. doi: 10.1016/0167-5699(92)90121-M. [DOI] [PubMed] [Google Scholar]
  13. MacDonald T. T., Carter P. B. Cell-mediated immunity to intestinal infection. Infect Immun. 1980 May;28(2):516–523. doi: 10.1128/iai.28.2.516-523.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marco A. J., Prats N., Ramos J. A., Briones V., Blanco M., Dominguez L., Domingo M. A microbiological, histopathological and immunohistological study of the intragastric inoculation of Listeria monocytogenes in mice. J Comp Pathol. 1992 Jul;107(1):1–9. doi: 10.1016/0021-9975(92)90090-h. [DOI] [PubMed] [Google Scholar]
  15. Okamoto M., Nakane A., Minagawa T. Host resistance to an intragastric infection with Listeria monocytogenes in mice depends on cellular immunity and intestinal bacterial flora. Infect Immun. 1994 Aug;62(8):3080–3085. doi: 10.1128/iai.62.8.3080-3085.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Portnoy D. A. Innate immunity to a facultative intracellular bacterial pathogen. Curr Opin Immunol. 1992 Feb;4(1):20–24. doi: 10.1016/0952-7915(92)90118-x. [DOI] [PubMed] [Google Scholar]
  17. Rakhmilevich A. L. Neutrophils are essential for resolution of primary and secondary infection with Listeria monocytogenes. J Leukoc Biol. 1995 Jun;57(6):827–831. doi: 10.1002/jlb.57.6.827. [DOI] [PubMed] [Google Scholar]
  18. Rogers H. W., Unanue E. R. Neutrophils are involved in acute, nonspecific resistance to Listeria monocytogenes in mice. Infect Immun. 1993 Dec;61(12):5090–5096. doi: 10.1128/iai.61.12.5090-5096.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Roll J. T., Czuprynski C. J. Hemolysin is required for extraintestinal dissemination of Listeria monocytogenes in intragastrically inoculated mice. Infect Immun. 1990 Sep;58(9):3147–3150. doi: 10.1128/iai.58.9.3147-3150.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schuchat A., Swaminathan B., Broome C. V. Epidemiology of human listeriosis. Clin Microbiol Rev. 1991 Apr;4(2):169–183. doi: 10.1128/cmr.4.2.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Smith J. A. Neutrophils, host defense, and inflammation: a double-edged sword. J Leukoc Biol. 1994 Dec;56(6):672–686. doi: 10.1002/jlb.56.6.672. [DOI] [PubMed] [Google Scholar]
  22. Szalay G., Hess J., Kaufmann S. H. Restricted replication of Listeria monocytogenes in a gamma interferon-activated murine hepatocyte line. Infect Immun. 1995 Aug;63(8):3187–3195. doi: 10.1128/iai.63.8.3187-3195.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Zachar Z., Savage D. C. Microbial interference and colonization of the murine gastrointestinal tract by Listeria monocytogenes. Infect Immun. 1979 Jan;23(1):168–174. doi: 10.1128/iai.23.1.168-174.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES