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. 1996 Oct;64(10):4356–4358. doi: 10.1128/iai.64.10.4356-4358.1996

Induction of heat shock protein 60 expression in human monocytic cell lines infected with Mycobacterium leprae.

K Beimnet 1, K Söderström 1, S Jindal 1, A Grönberg 1, D Frommel 1, R Kiessling 1
PMCID: PMC174379  PMID: 8926111

Abstract

Monocytic cell lines (HL-60 and THP-1) were infected with viable Mycobacterium leprae. Levels of human hsp60 were estimated by Western blot (immunoblot) assay and a sandwich enzyme-linked immunosorbent assay. The results showed that infection of both of the cell lines induced the synthesis of human hsp60, which may be of significance in relation to autoimmune manifestations associated with mycobacterial infections.

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Selected References

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  1. Boog C. J., de Graeff-Meeder E. R., Lucassen M. A., van der Zee R., Voorhorst-Ogink M. M., van Kooten P. J., Geuze H. J., van Eden W. Two monoclonal antibodies generated against human hsp60 show reactivity with synovial membranes of patients with juvenile chronic arthritis. J Exp Med. 1992 Jun 1;175(6):1805–1810. doi: 10.1084/jem.175.6.1805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chirico W. J., Waters M. G., Blobel G. 70K heat shock related proteins stimulate protein translocation into microsomes. Nature. 1988 Apr 28;332(6167):805–810. doi: 10.1038/332805a0. [DOI] [PubMed] [Google Scholar]
  3. Deitch E. A., Beck S. C., Cruz N. C., De Maio A. Induction of heat shock gene expression in colonic epithelial cells after incubation with Escherichia coli or endotoxin. Crit Care Med. 1995 Aug;23(8):1371–1376. doi: 10.1097/00003246-199508000-00010. [DOI] [PubMed] [Google Scholar]
  4. Ferm M. T., Söderström K., Jindal S., Grönberg A., Ivanyi J., Young R., Kiessling R. Induction of human hsp60 expression in monocytic cell lines. Int Immunol. 1992 Mar;4(3):305–311. doi: 10.1093/intimm/4.3.305. [DOI] [PubMed] [Google Scholar]
  5. Franzblau S. G. Oxidation of palmitic acid by Mycobacterium leprae in an axenic medium. J Clin Microbiol. 1988 Jan;26(1):18–21. doi: 10.1128/jcm.26.1.18-21.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Garsia R. J., Hellqvist L., Booth R. J., Radford A. J., Britton W. J., Astbury L., Trent R. J., Basten A. Homology of the 70-kilodalton antigens from Mycobacterium leprae and Mycobacterium bovis with the Mycobacterium tuberculosis 71-kilodalton antigen and with the conserved heat shock protein 70 of eucaryotes. Infect Immun. 1989 Jan;57(1):204–212. doi: 10.1128/iai.57.1.204-212.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ivanyi J., Sinha S., Aston R., Cussell D., Keen M., Sengupta U. Definition of species specific and cross-reactive antigenic determinants of Mycobacterium leprae using monoclonal antibodies. Clin Exp Immunol. 1983 Jun;52(3):528–536. [PMC free article] [PubMed] [Google Scholar]
  8. Jindal S., Burke E., Bettencourt B., Khandekar S., Young R. A., Dwyer D. S. Human Hsp60: bacterial expression, purification, development of monoclonal antibodies and a sandwich ELISA for quantitation. Immunol Lett. 1994 Feb;39(2):127–135. doi: 10.1016/0165-2478(94)90097-3. [DOI] [PubMed] [Google Scholar]
  9. Kantengwa S., Müller I., Louis J., Polla B. S. Infection of human and murine macrophages with Leishmania major is associated with early parasite heat shock protein synthesis but fails to induce a host cell stress response. Immunol Cell Biol. 1995 Feb;73(1):73–80. doi: 10.1038/icb.1995.12. [DOI] [PubMed] [Google Scholar]
  10. Kaplan G., Cohn Z. A. The immunobiology of leprosy. Int Rev Exp Pathol. 1986;28:45–78. [PubMed] [Google Scholar]
  11. Karlsson-Parra A., Söderström K., Ferm M., Ivanyi J., Kiessling R., Klareskog L. Presence of human 65 kD heat shock protein (hsp) in inflamed joints and subcutaneous nodules of RA patients. Scand J Immunol. 1990 Jun;31(6):283–288. doi: 10.1111/j.1365-3083.1990.tb02770.x. [DOI] [PubMed] [Google Scholar]
  12. Kaufmann S. H. Immunology of leprosy: new findings, future perspectives. Microb Pathog. 1986 Apr;1(2):107–114. doi: 10.1016/0882-4010(86)90013-6. [DOI] [PubMed] [Google Scholar]
  13. Koga T., Wand-Württenberger A., DeBruyn J., Munk M. E., Schoel B., Kaufmann S. H. T cells against a bacterial heat shock protein recognize stressed macrophages. Science. 1989 Sep 8;245(4922):1112–1115. doi: 10.1126/science.2788923. [DOI] [PubMed] [Google Scholar]
  14. Köller M., Hensler T., König B., Prévost G., Alouf J., König W. Induction of heat-shock proteins by bacterial toxins, lipid mediators and cytokines in human leukocytes. Zentralbl Bakteriol. 1993 Apr;278(2-3):365–376. doi: 10.1016/s0934-8840(11)80853-4. [DOI] [PubMed] [Google Scholar]
  15. Lamb J. R., Bal V., Mendez-Samperio P., Mehlert A., So A., Rothbard J., Jindal S., Young R. A., Young D. B. Stress proteins may provide a link between the immune response to infection and autoimmunity. Int Immunol. 1989;1(2):191–196. doi: 10.1093/intimm/1.2.191. [DOI] [PubMed] [Google Scholar]
  16. Lindquist S., Craig E. A. The heat-shock proteins. Annu Rev Genet. 1988;22:631–677. doi: 10.1146/annurev.ge.22.120188.003215. [DOI] [PubMed] [Google Scholar]
  17. Lydyard P. M., van Eden W. Heat shock proteins: immunity and immunopathology. Immunol Today. 1990 Jul;11(7):228–229. doi: 10.1016/0167-5699(90)90091-m. [DOI] [PubMed] [Google Scholar]
  18. Mori T., Miyata Y., Yoneda K., Ito T. Collection method for Mycobacterium leprae from infected armadillo liver. Int J Lepr Other Mycobact Dis. 1984 Mar;52(1):41–43. [PubMed] [Google Scholar]
  19. Munk M. E., Schoel B., Modrow S., Karr R. W., Young R. A., Kaufmann S. H. T lymphocytes from healthy individuals with specificity to self-epitopes shared by the mycobacterial and human 65-kilodalton heat shock protein. J Immunol. 1989 Nov 1;143(9):2844–2849. [PubMed] [Google Scholar]
  20. Russell W. C., Newman C., Williamson D. H. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature. 1975 Feb 6;253(5491):461–462. doi: 10.1038/253461a0. [DOI] [PubMed] [Google Scholar]
  21. Sedger L., Ruby J. Heat shock response to vaccinia virus infection. J Virol. 1994 Jul;68(7):4685–4689. doi: 10.1128/jvi.68.7.4685-4689.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]

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