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. 1996 Nov;64(11):4612–4617. doi: 10.1128/iai.64.11.4612-4617.1996

Restoration of lipopolysaccharide-mediated B-cell response after expression of a cDNA encoding a GTP-binding protein.

A D Kang 1, P M Wong 1, H Chen 1, R Castagna 1, S W Chung 1, B M Sultzer 1
PMCID: PMC174421  PMID: 8890215

Abstract

Previous analysis of hybrid progeny derived from lipopolysaccharide (LPS) responder and nonresponder inbred mouse strains demonstrated that a single genetic locus controlled responsiveness to LPS. Using a differential functional screening approach, we report the isolation of a cDNA that has sequence homology to a GTP-binding protein. Expression of the cDNA in splenic B cells of C3H/HeJ nonresponder, endotoxin-resistant mice resulted in polyclonal B-cell activation in response to LPS stimulation. Thus a GTP-binding protein may be involved in LPS stimulation in B cells and perhaps other cell types.

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Selected References

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