Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1996 Nov;64(11):4830–4833. doi: 10.1128/iai.64.11.4830-4833.1996

Role of neutrophils in controlling early stages of a Chlamydia trachomatis infection.

N Barteneva 1, I Theodor 1, E M Peterson 1, L M de la Maza 1
PMCID: PMC174452  PMID: 8890246

Abstract

Inoculation of mice with the granulocyte-depleting monoclonal antibody RB6-8C5 showed that neutrophils are critical protective effector cells during a Chlamydia trachomatis infection. In addition, administration of monoclonal antibody 2E6 demonstrated that extravasation of neutrophils into the peritoneal cavity in response to inoculation with the C. trachomatis MoPn biovar is dependent on the surface beta-2 integrin molecule CD18.

Full Text

The Full Text of this article is available as a PDF (179.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arya O. P., Mallinson H., Andrews B. E., Sillis M. Diagnosis of urethritis: role of polymorphonuclear leukocyte counts in gram-stained urethral smears. Sex Transm Dis. 1984 Jan-Mar;11(1):10–17. [PubMed] [Google Scholar]
  2. Beem M. O., Saxon E. M. Respiratory-tract colonization and a distinctive pneumonia syndrome in infants infected with Chlamydia trachomatis. N Engl J Med. 1977 Feb 10;296(6):306–310. doi: 10.1056/NEJM197702102960604. [DOI] [PubMed] [Google Scholar]
  3. Brunham R. C., Binns B., Guijon F., Danforth D., Kosseim M. L., Rand F., McDowell J., Rayner E. Etiology and outcome of acute pelvic inflammatory disease. J Infect Dis. 1988 Sep;158(3):510–517. doi: 10.1093/infdis/158.3.510. [DOI] [PubMed] [Google Scholar]
  4. Brunham R. C., Paavonen J., Stevens C. E., Kiviat N., Kuo C. C., Critchlow C. W., Holmes K. K. Mucopurulent cervicitis--the ignored counterpart in women of urethritis in men. N Engl J Med. 1984 Jul 5;311(1):1–6. doi: 10.1056/NEJM198407053110101. [DOI] [PubMed] [Google Scholar]
  5. Conlan J. W., North R. J. Listeria monocytogenes, but not Salmonella typhimurium, elicits a CD18-independent mechanism of neutrophil extravasation into the murine peritoneal cavity. Infect Immun. 1994 Jul;62(7):2702–2706. doi: 10.1128/iai.62.7.2702-2706.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Conlan J. W., North R. J. Neutrophil-mediated dissolution of infected host cells as a defense strategy against a facultative intracellular bacterium. J Exp Med. 1991 Sep 1;174(3):741–744. doi: 10.1084/jem.174.3.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Conlan J. W., North R. J. Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody. J Exp Med. 1994 Jan 1;179(1):259–268. doi: 10.1084/jem.179.1.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Czuprynski C. J., Brown J. F., Maroushek N., Wagner R. D., Steinberg H. Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection. J Immunol. 1994 Feb 15;152(4):1836–1846. [PubMed] [Google Scholar]
  9. D'Cruz O. J., Haas G. G., Jr Beta 2-integrin (CD11b/CD18) is the primary adhesive glycoprotein complex involved in neutrophil-mediated immune injury to human sperm. Biol Reprod. 1995 Nov;53(5):1118–1130. doi: 10.1095/biolreprod53.5.1118. [DOI] [PubMed] [Google Scholar]
  10. Doerschuk C. M., Winn R. K., Coxson H. O., Harlan J. M. CD18-dependent and -independent mechanisms of neutrophil emigration in the pulmonary and systemic microcirculation of rabbits. J Immunol. 1990 Mar 15;144(6):2327–2333. [PubMed] [Google Scholar]
  11. Fleming T. J., Fleming M. L., Malek T. R. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993 Sep 1;151(5):2399–2408. [PubMed] [Google Scholar]
  12. La Verda D., Byrne G. I. Interactions between macrophages and chlamydiae. Immunol Ser. 1994;60:381–399. [PubMed] [Google Scholar]
  13. Lloyd A. R., Oppenheim J. J. Poly's lament: the neglected role of the polymorphonuclear neutrophil in the afferent limb of the immune response. Immunol Today. 1992 May;13(5):169–172. doi: 10.1016/0167-5699(92)90121-M. [DOI] [PubMed] [Google Scholar]
  14. Manor E., Sarov I. Fate of Chlamydia trachomatis in human monocytes and monocyte-derived macrophages. Infect Immun. 1986 Oct;54(1):90–95. doi: 10.1128/iai.54.1.90-95.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Metlay J. P., Witmer-Pack M. D., Agger R., Crowley M. T., Lawless D., Steinman R. M. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J Exp Med. 1990 May 1;171(5):1753–1771. doi: 10.1084/jem.171.5.1753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Morrison R. P., Feilzer K., Tumas D. B. Gene knockout mice establish a primary protective role for major histocompatibility complex class II-restricted responses in Chlamydia trachomatis genital tract infection. Infect Immun. 1995 Dec;63(12):4661–4668. doi: 10.1128/iai.63.12.4661-4668.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moulder J. W. Interaction of chlamydiae and host cells in vitro. Microbiol Rev. 1991 Mar;55(1):143–190. doi: 10.1128/mr.55.1.143-190.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pal S., Fielder T. J., Peterson E. M., de la Maza L. M. Analysis of the immune response in mice following intrauterine infection with the Chlamydia trachomatis mouse pneumonitis biovar. Infect Immun. 1993 Feb;61(2):772–776. doi: 10.1128/iai.61.2.772-776.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pal S., Fielder T. J., Peterson E. M., de la Maza L. M. Protection against infertility in a BALB/c mouse salpingitis model by intranasal immunization with the mouse pneumonitis biovar of Chlamydia trachomatis. Infect Immun. 1994 Aug;62(8):3354–3362. doi: 10.1128/iai.62.8.3354-3362.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rogers H. W., Unanue E. R. Neutrophils are involved in acute, nonspecific resistance to Listeria monocytogenes in mice. Infect Immun. 1993 Dec;61(12):5090–5096. doi: 10.1128/iai.61.12.5090-5096.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sjöstedt A., Conlan J. W., North R. J. Neutrophils are critical for host defense against primary infection with the facultative intracellular bacterium Francisella tularensis in mice and participate in defense against reinfection. Infect Immun. 1994 Jul;62(7):2779–2783. doi: 10.1128/iai.62.7.2779-2783.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tepper R. I., Coffman R. L., Leder P. An eosinophil-dependent mechanism for the antitumor effect of interleukin-4. Science. 1992 Jul 24;257(5069):548–551. doi: 10.1126/science.1636093. [DOI] [PubMed] [Google Scholar]
  23. Thomas D. D., Sharar S. R., Winn R. K., Chi E. Y., Verrier E. D., Allen M. D., Bishop M. J. CD18-independent mechanism of neutrophil emigration in the rabbit lung after ischemia-reperfusion. Ann Thorac Surg. 1995 Nov;60(5):1360–1366. doi: 10.1016/0003-4975(95)00546-W. [DOI] [PubMed] [Google Scholar]
  24. Tumpey T. M., Chen S. H., Oakes J. E., Lausch R. N. Neutrophil-mediated suppression of virus replication after herpes simplex virus type 1 infection of the murine cornea. J Virol. 1996 Feb;70(2):898–904. doi: 10.1128/jvi.70.2.898-904.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Weström L., Joesoef R., Reynolds G., Hagdu A., Thompson S. E. Pelvic inflammatory disease and fertility. A cohort study of 1,844 women with laparoscopically verified disease and 657 control women with normal laparoscopic results. Sex Transm Dis. 1992 Jul-Aug;19(4):185–192. [PubMed] [Google Scholar]
  26. Yoneda C., Dawson C. R., Daghfous T., Hoshiwara I., Jones P., Messadi M., Schachter J. Cytology as a guide to the presence of chlamydial inclusions in Giemsa-stained conjunctival smears in severe endemic trachoma. Br J Ophthalmol. 1975 Mar;59(3):116–124. doi: 10.1136/bjo.59.3.116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Yong E. C., Chi E. Y., Chen W. J., Kuo C. C. Degradation of Chlamydia trachomatis in human polymorphonuclear leukocytes: an ultrastructural study of peroxidase-positive phagolysosomes. Infect Immun. 1986 Aug;53(2):427–431. doi: 10.1128/iai.53.2.427-431.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Yong E. C., Klebanoff S. J., Kuo C. C. Toxic effect of human polymorphonuclear leukocytes on Chlamydia trachomatis. Infect Immun. 1982 Aug;37(2):422–426. doi: 10.1128/iai.37.2.422-426.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Zhong G. M., Peterson E. M., Czarniecki C. W., de la Maza L. M. Recombinant murine gamma interferon inhibits Chlamydia trachomatis serovar L1 in vivo. Infect Immun. 1988 Jan;56(1):283–286. doi: 10.1128/iai.56.1.283-286.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES