Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1996 Dec;64(12):5390–5394. doi: 10.1128/iai.64.12.5390-5394.1996

Influence of cloned tRNA genes from a uropathogenic Escherichia coli strain on adherence to primary human renal tubular epithelial cells and nephropathogenicity in rats.

M Susa 1, B Kreft 1, G Wasenauer 1, A Ritter 1, J Hacker 1, R Marre 1
PMCID: PMC174534  PMID: 8945592

Abstract

The uropathogenic Escherichia coli strain 536 (O6:K15:H31) possesses pathogenicity islands which are incorporated into two tRNA genes, the selC and the leuX gene. The leuX gene influences the expression of different putative virulence factors. We demonstrate an effect of the leuX-specific tRNA on adherence and uropathogenicity.

Full Text

The Full Text of this article is available as a PDF (620.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison C., Coleman N., Jones P. L., Hughes C. Ability of Proteus mirabilis to invade human urothelial cells is coupled to motility and swarming differentiation. Infect Immun. 1992 Nov;60(11):4740–4746. doi: 10.1128/iai.60.11.4740-4746.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berger H., Hacker J., Juarez A., Hughes C., Goebel W. Cloning of the chromosomal determinants encoding hemolysin production and mannose-resistant hemagglutination in Escherichia coli. J Bacteriol. 1982 Dec;152(3):1241–1247. doi: 10.1128/jb.152.3.1241-1247.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blum G., Falbo V., Caprioli A., Hacker J. Gene clusters encoding the cytotoxic necrotizing factor type 1, Prs-fimbriae and alpha-hemolysin form the pathogenicity island II of the uropathogenic Escherichia coli strain J96. FEMS Microbiol Lett. 1995 Feb 15;126(2):189–195. doi: 10.1111/j.1574-6968.1995.tb07415.x. [DOI] [PubMed] [Google Scholar]
  4. Blum G., Ott M., Lischewski A., Ritter A., Imrich H., Tschäpe H., Hacker J. Excision of large DNA regions termed pathogenicity islands from tRNA-specific loci in the chromosome of an Escherichia coli wild-type pathogen. Infect Immun. 1994 Feb;62(2):606–614. doi: 10.1128/iai.62.2.606-614.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Böck A., Forchhammer K., Heider J., Leinfelder W., Sawers G., Veprek B., Zinoni F. Selenocysteine: the 21st amino acid. Mol Microbiol. 1991 Mar;5(3):515–520. doi: 10.1111/j.1365-2958.1991.tb00722.x. [DOI] [PubMed] [Google Scholar]
  6. Crosa J. H. Genetics and molecular biology of siderophore-mediated iron transport in bacteria. Microbiol Rev. 1989 Dec;53(4):517–530. doi: 10.1128/mr.53.4.517-530.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Detrisac C. J., Sens M. A., Garvin A. J., Spicer S. S., Sens D. A. Tissue culture of human kidney epithelial cells of proximal tubule origin. Kidney Int. 1984 Feb;25(2):383–390. doi: 10.1038/ki.1984.28. [DOI] [PubMed] [Google Scholar]
  8. Hacker J., Bender L., Ott M., Wingender J., Lund B., Marre R., Goebel W. Deletions of chromosomal regions coding for fimbriae and hemolysins occur in vitro and in vivo in various extraintestinal Escherichia coli isolates. Microb Pathog. 1990 Mar;8(3):213–225. doi: 10.1016/0882-4010(90)90048-u. [DOI] [PubMed] [Google Scholar]
  9. Hacker J. Genetic determinants coding for fimbriae and adhesins of extraintestinal Escherichia coli. Curr Top Microbiol Immunol. 1990;151:1–27. doi: 10.1007/978-3-642-74703-8_1. [DOI] [PubMed] [Google Scholar]
  10. Hacker J., Knapp S., Goebel W. Spontaneous deletions and flanking regions of the chromosomally inherited hemolysin determinant of an Escherichia coli O6 strain. J Bacteriol. 1983 Jun;154(3):1145–1152. doi: 10.1128/jb.154.3.1145-1152.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Henkel W., Commichau R. Serumresistenz und "Nephropathogenität" antigenetisch definierter Stämme von E. coli. 3. Experimentelle Pyelonephritis mit unterschiedlich serumresistenten Keimen. Z Med Mikrobiol Immunol. 1971;156(3):297–304. [PubMed] [Google Scholar]
  12. Hughes C., Hacker J., Düvel H., Goebel W. Chromosomal deletions and rearrangements cause coordinate loss of haemolysis, fimbriation and serum resistance in a uropathogenic strain of Escherichia coli. Microb Pathog. 1987 Mar;2(3):227–230. doi: 10.1016/0882-4010(87)90024-6. [DOI] [PubMed] [Google Scholar]
  13. Knapp S., Hacker J., Jarchau T., Goebel W. Large, unstable inserts in the chromosome affect virulence properties of uropathogenic Escherichia coli O6 strain 536. J Bacteriol. 1986 Oct;168(1):22–30. doi: 10.1128/jb.168.1.22-30.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Komine Y., Adachi T., Inokuchi H., Ozeki H. Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12. J Mol Biol. 1990 Apr 20;212(4):579–598. doi: 10.1016/0022-2836(90)90224-A. [DOI] [PubMed] [Google Scholar]
  15. Kreft B., Placzek M., Doehn C., Hacker J., Schmidt G., Wasenauer G., Daha M. R., van der Woude F. J., Sack K. S fimbriae of uropathogenic Escherichia coli bind to primary human renal proximal tubular epithelial cells but do not induce expression of intercellular adhesion molecule 1. Infect Immun. 1995 Aug;63(8):3235–3238. doi: 10.1128/iai.63.8.3235-3238.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Leying H., Suerbaum S., Geis G., Haas R. Cloning and genetic characterization of a Helicobacter pylori flagellin gene. Mol Microbiol. 1992 Oct;6(19):2863–2874. doi: 10.1111/j.1365-2958.1992.tb01466.x. [DOI] [PubMed] [Google Scholar]
  17. Lähteenmäki K., Virkola R., Pouttu R., Kuusela P., Kukkonen M., Korhonen T. K. Bacterial plasminogen receptors: in vitro evidence for a role in degradation of the mammalian extracellular matrix. Infect Immun. 1995 Sep;63(9):3659–3664. doi: 10.1128/iai.63.9.3659-3664.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Marre R., Hacker J., Henkel W., Goebel W. Contribution of cloned virulence factors from uropathogenic Escherichia coli strains to nephropathogenicity in an experimental rat pyelonephritis model. Infect Immun. 1986 Dec;54(3):761–767. doi: 10.1128/iai.54.3.761-767.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Marre R., Kreft B., Hacker J. Genetically engineered S and F1C fimbriae differ in their contribution to adherence of Escherichia coli to cultured renal tubular cells. Infect Immun. 1990 Oct;58(10):3434–3437. doi: 10.1128/iai.58.10.3434-3437.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. McDaniel T. K., Jarvis K. G., Donnenberg M. S., Kaper J. B. A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1664–1668. doi: 10.1073/pnas.92.5.1664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Morschhäuser J., Vetter V., Emödy L., Hacker J. Adhesin regulatory genes within large, unstable DNA regions of pathogenic Escherichia coli: cross-talk between different adhesin gene clusters. Mol Microbiol. 1994 Feb;11(3):555–566. doi: 10.1111/j.1365-2958.1994.tb00336.x. [DOI] [PubMed] [Google Scholar]
  22. Mühldorfer I., Hacker J. Genetic aspects of Escherichia coli virulence. Microb Pathog. 1994 Mar;16(3):171–181. doi: 10.1006/mpat.1994.1018. [DOI] [PubMed] [Google Scholar]
  23. Newman J. V., Kolter R., Laux D. C., Cohen P. S. Role of leuX in Escherichia coli colonization of the streptomycin-treated mouse large intestine. Microb Pathog. 1994 Nov;17(5):301–311. doi: 10.1006/mpat.1994.1076. [DOI] [PubMed] [Google Scholar]
  24. Ritter A., Blum G., Emödy L., Kerenyi M., Böck A., Neuhierl B., Rabsch W., Scheutz F., Hacker J. tRNA genes and pathogenicity islands: influence on virulence and metabolic properties of uropathogenic Escherichia coli. Mol Microbiol. 1995 Jul;17(1):109–121. doi: 10.1111/j.1365-2958.1995.mmi_17010109.x. [DOI] [PubMed] [Google Scholar]
  25. Sack K., Freiesleben H., Wöhrmann W., Henkel W., Commichau R. Experimenteller Beitrag zum Infektionsweg der coli-Pyelonephritis. Virchows Arch A Pathol Pathol Anat. 1972;355(4):323–332. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES