Abstract
We have devised a procedure that permits the cultivation of a gram-positive coccoid species from biopsy material obtained from the antrum of the stomachs of patients with gastric disorders. Antibodies directed against surface proteins obtained from the coccoid isolates were detected in all patients with gastric disorders examined in this study, including both Helicobacter pylori-infected and H. pylori-uninfected patients. Several of these isolates, including a prototype designated strain SL100, have been characterized in some detail. Strain SL100 exhibits urease and exceptionally high catalase activities and assumes a variety of spherical morphologies as detected by electron microscopy. This isolate expresses an adhesin that binds to gastric mucin. The adhesin activity was detected only after the isolate was exposed to an acidic pH, suggesting that in the natural process of infection, the low pH of the stomach unmasks a cell surface component with adhesin activity. Strain SL100 grows best under a microaerophilic conditions (10% CO2, 5% O2, 85% N2), but it also grows quite well under aerobic conditions. Thus, this organism would be expected to proliferate outside of the human host as well as in the gastric mucosa. Oral infection of newborn piglets resulted in colonization of the gastric antrum and growth retardation. Preliminary taxonomic classification indicates similarity to the Staphylococcus DNA homology groups containing S. cohnii and S. xylosus. One of us (C.K.) apparently became infected with this organism as indicated by gastric symptoms and the subsequent presence of strain-specific antisera not present in other workers in the laboratory.
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- Bode G., Malfertheiner P., Ditschuneit H. Pathogenetic implications of ultrastructural findings in Campylobacter pylori related gastroduodenal disease. Scand J Gastroenterol Suppl. 1988;142:25–39. [PubMed] [Google Scholar]
- Crespo I., Murphy J., Wong R. K. Superior mesenteric venous thrombosis masquerading as Crohn's disease. Am J Gastroenterol. 1994 Jan;89(1):116–118. [PubMed] [Google Scholar]
- Eaton K. A., Catrenich C. E., Makin K. M., Krakowka S. Virulence of coccoid and bacillary forms of Helicobacter pylori in gnotobiotic piglets. J Infect Dis. 1995 Feb;171(2):459–462. doi: 10.1093/infdis/171.2.459. [DOI] [PubMed] [Google Scholar]
- Eaton K. A., Krakowka S. Effect of gastric pH on urease-dependent colonization of gnotobiotic piglets by Helicobacter pylori. Infect Immun. 1994 Sep;62(9):3604–3607. doi: 10.1128/iai.62.9.3604-3607.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Engstrand L., Rosberg K., Hübinette R., Berglindh T., Rolfsen W., Gustavsson S. Topographic mapping of Helicobacter pylori colonization in long-term-infected pigs. Infect Immun. 1992 Feb;60(2):653–656. doi: 10.1128/iai.60.2.653-656.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans D. G., Evans D. J., Jr, Graham D. Y. Adherence and internalization of Helicobacter pylori by HEp-2 cells. Gastroenterology. 1992 May;102(5):1557–1567. doi: 10.1016/0016-5085(92)91714-f. [DOI] [PubMed] [Google Scholar]
- Haas A., Goebel W. Microbial strategies to prevent oxygen-dependent killing by phagocytes. Free Radic Res Commun. 1992;16(3):137–157. doi: 10.3109/10715769209049167. [DOI] [PubMed] [Google Scholar]
- Hansing R. L., D'Amico H., Levy M., Guillan R. A. Prediction of Helicobacter pylori in gastric specimens by inflammatory and morphological histological evaluation. Am J Gastroenterol. 1992 Sep;87(9):1125–1131. [PubMed] [Google Scholar]
- Kloos W. E. Natural populations of the genus Staphylococcus. Annu Rev Microbiol. 1980;34:559–592. doi: 10.1146/annurev.mi.34.100180.003015. [DOI] [PubMed] [Google Scholar]
- Morrison M. Lactoperoxidase-catalyzed iodination as a tool for investigation of proteins. Methods Enzymol. 1980;70(A):214–220. doi: 10.1016/s0076-6879(80)70051-4. [DOI] [PubMed] [Google Scholar]
- Nagata K., Mizuta T., Tonokatu Y., Fukuda Y., Okamura H., Hayashi T., Shimoyama T., Tamura T. Monoclonal antibodies against the native urease of Helicobacter pylori: synergistic inhibition of urease activity by monoclonal antibody combinations. Infect Immun. 1992 Nov;60(11):4826–4831. doi: 10.1128/iai.60.11.4826-4831.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nielsen H., Andersen L. P. Activation of human phagocyte oxidative metabolism by Helicobacter pylori. Gastroenterology. 1992 Dec;103(6):1747–1753. doi: 10.1016/0016-5085(92)91430-c. [DOI] [PubMed] [Google Scholar]
- Olivieri R., Bugnoli M., Armellini D., Bianciardi S., Rappuoli R., Bayeli P. F., Abate L., Esposito E., de Gregorio L., Aziz J. Growth of Helicobacter pylori in media containing cyclodextrins. J Clin Microbiol. 1993 Jan;31(1):160–162. doi: 10.1128/jcm.31.1.160-162.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pérez-Pérez G. I., Olivares A. Z., Cover T. L., Blaser M. J. Characteristics of Helicobacter pylori variants selected for urease deficiency. Infect Immun. 1992 Sep;60(9):3658–3663. doi: 10.1128/iai.60.9.3658-3663.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rudmann D. G., Eaton K. A., Krakowka S. Ultrastructural study of Helicobacter pylori adherence properties in gnotobiotic piglets. Infect Immun. 1992 May;60(5):2121–2124. doi: 10.1128/iai.60.5.2121-2124.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Talley N. J., Ormand J. E., Frie C. A., Zinsmeister A. R. Stability of pH gradients in vivo across the stomach in Helicobacter pylori gastritis, dyspepsia, and health. Am J Gastroenterol. 1992 May;87(5):590–594. [PubMed] [Google Scholar]