Skip to main content
PMC home page
Thorax logoLink to Thorax
. 1999 Sep;54(9):805–812. doi: 10.1136/thx.54.9.805

Reduction of bleomycin induced lung fibrosis by transforming growth factor β soluble receptor in hamsters

Q Wang 1, Y Wang 1, D Hyde 1, P Gotwals 1, V Koteliansky 1, S Ryan 1, S Giri 1
PMCID: PMC1745567  PMID: 10456973

Full Text

The Full Text of this article is available as a PDF (160.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Border W. A., Noble N. A. Cytokines in kidney disease: the role of transforming growth factor-beta. Am J Kidney Dis. 1993 Jul;22(1):105–113. doi: 10.1016/s0272-6386(12)70175-0. [DOI] [PubMed] [Google Scholar]
  2. Border W. A., Noble N. A. Transforming growth factor beta in tissue fibrosis. N Engl J Med. 1994 Nov 10;331(19):1286–1292. doi: 10.1056/NEJM199411103311907. [DOI] [PubMed] [Google Scholar]
  3. Border W. A., Ruoslahti E. Transforming growth factor-beta in disease: the dark side of tissue repair. J Clin Invest. 1992 Jul;90(1):1–7. doi: 10.1172/JCI115821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bowden D. H. Unraveling pulmonary fibrosis: the bleomycin model. Lab Invest. 1984 May;50(5):487–488. [PubMed] [Google Scholar]
  5. Broekelmann T. J., Limper A. H., Colby T. V., McDonald J. A. Transforming growth factor beta 1 is present at sites of extracellular matrix gene expression in human pulmonary fibrosis. Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6642–6646. doi: 10.1073/pnas.88.15.6642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clark D. A., Coker R. Transforming growth factor-beta (TGF-beta). Int J Biochem Cell Biol. 1998 Mar;30(3):293–298. doi: 10.1016/s1357-2725(97)00128-3. [DOI] [PubMed] [Google Scholar]
  7. Coker R. K., Laurent G. J., Shahzeidi S., Lympany P. A., du Bois R. M., Jeffery P. K., McAnulty R. J. Transforming growth factors-beta 1, -beta 2, and -beta 3 stimulate fibroblast procollagen production in vitro but are differentially expressed during bleomycin-induced lung fibrosis. Am J Pathol. 1997 Mar;150(3):981–991. [PMC free article] [PubMed] [Google Scholar]
  8. Crippen T. L., Klasing K. C., Hyde D. M. Regulation of cytokine-induced neutrophil chemoattractant in rat bone marrow-derived macrophages by inflammatory mediators. Pathobiology. 1998;66(6):293–301. doi: 10.1159/000028036. [DOI] [PubMed] [Google Scholar]
  9. Crippen T. L., Riches D. W., Hyde D. M. Differential regulation of the expression of cytokine-induced neutrophil chemoattractant by mouse macrophages. Pathobiology. 1998;66(1):24–32. doi: 10.1159/000027991. [DOI] [PubMed] [Google Scholar]
  10. Ebner R., Chen R. H., Lawler S., Zioncheck T., Derynck R. Determination of type I receptor specificity by the type II receptors for TGF-beta or activin. Science. 1993 Nov 5;262(5135):900–902. doi: 10.1126/science.8235612. [DOI] [PubMed] [Google Scholar]
  11. Fadok V. A., Bratton D. L., Konowal A., Freed P. W., Westcott J. Y., Henson P. M. Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF. J Clin Invest. 1998 Feb 15;101(4):890–898. doi: 10.1172/JCI1112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ghahary A., Shen Y. J., Scott P. G., Gong Y., Tredget E. E. Enhanced expression of mRNA for transforming growth factor-beta, type I and type III procollagen in human post-burn hypertrophic scar tissues. J Lab Clin Med. 1993 Oct;122(4):465–473. [PubMed] [Google Scholar]
  13. Giri S. N., Hyde D. M., Braun R. K., Gaarde W., Harper J. R., Pierschbacher M. D. Antifibrotic effect of decorin in a bleomycin hamster model of lung fibrosis. Biochem Pharmacol. 1997 Dec 1;54(11):1205–1216. doi: 10.1016/s0006-2952(97)00343-2. [DOI] [PubMed] [Google Scholar]
  14. Giri S. N., Hyde D. M., Hollinger M. A. Effect of antibody to transforming growth factor beta on bleomycin induced accumulation of lung collagen in mice. Thorax. 1993 Oct;48(10):959–966. doi: 10.1136/thx.48.10.959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Giri S. N., Misra H. P., Chandler D. B., Chen Z. L. Increases in lung prolyl hydroxylase and superoxide dismutase activities during bleomycin-induced lung fibrosis in hamsters. Exp Mol Pathol. 1983 Dec;39(3):317–326. doi: 10.1016/0014-4800(83)90060-6. [DOI] [PubMed] [Google Scholar]
  16. Glansbeek H. L., van Beuningen H. M., Vitters E. L., van der Kraan P. M., van den Berg W. B. Expression of recombinant human soluble type II transforming growth factor-beta receptor in Pichia pastoris and Escherichia coli: two powerful systems to express a potent inhibitor of transforming growth factor-beta. Protein Expr Purif. 1998 Mar;12(2):201–207. doi: 10.1006/prep.1997.0819. [DOI] [PubMed] [Google Scholar]
  17. Goetschy J. F., Letourneur O., Cerletti N., Horisberger M. A. The unglycosylated extracellular domain of type-II receptor for transforming growth factor-beta. A novel assay for characterizing ligand affinity and specificity. Eur J Biochem. 1996 Oct 15;241(2):355–362. doi: 10.1111/j.1432-1033.1996.00355.x. [DOI] [PubMed] [Google Scholar]
  18. Gurujeyalakshmi G., Hollinger M. A., Giri S. N. Regulation of transforming growth factor-beta1 mRNA expression by taurine and niacin in the bleomycin hamster model of lung fibrosis. Am J Respir Cell Mol Biol. 1998 Mar;18(3):334–342. doi: 10.1165/ajrcmb.18.3.2867. [DOI] [PubMed] [Google Scholar]
  19. Harris J. A., Hyde D. M., Wang Q. J., Stovall M. Y., Giri S. N. Repeated episodes of C5a-induced neutrophil influx do not result in pulmonary fibrosis. Inflammation. 1991 Jun;15(3):233–250. doi: 10.1007/BF00918649. [DOI] [PubMed] [Google Scholar]
  20. Hoyt D. G., Lazo J. S. Alterations in pulmonary mRNA encoding procollagens, fibronectin and transforming growth factor-beta precede bleomycin-induced pulmonary fibrosis in mice. J Pharmacol Exp Ther. 1988 Aug;246(2):765–771. [PubMed] [Google Scholar]
  21. Isaka Y., Akagi Y., Ando Y., Tsujie M., Sudo T., Ohno N., Border W. A., Noble N. A., Kaneda Y., Hori M. Gene therapy by transforming growth factor-beta receptor-IgG Fc chimera suppressed extracellular matrix accumulation in experimental glomerulonephritis. Kidney Int. 1999 Feb;55(2):465–475. doi: 10.1046/j.1523-1755.1999.00275.x. [DOI] [PubMed] [Google Scholar]
  22. Karlinsky J. B., Goldstein R. H. Fibrotic lung disease--a perspective. J Lab Clin Med. 1980 Dec;96(6):939–942. [PubMed] [Google Scholar]
  23. Kelley J., Newman R. A., Evans J. N. Bleomycin-induced pulmonary fibrosis in the rat. Prevention with an inhibitor of collagen synthesis. J Lab Clin Med. 1980 Dec;96(6):954–964. [PubMed] [Google Scholar]
  24. Khalil N., Bereznay O., Sporn M., Greenberg A. H. Macrophage production of transforming growth factor beta and fibroblast collagen synthesis in chronic pulmonary inflammation. J Exp Med. 1989 Sep 1;170(3):727–737. doi: 10.1084/jem.170.3.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Kingsley D. M. The TGF-beta superfamily: new members, new receptors, and new genetic tests of function in different organisms. Genes Dev. 1994 Jan;8(2):133–146. doi: 10.1101/gad.8.2.133. [DOI] [PubMed] [Google Scholar]
  26. Komesli S., Vivien D., Dutartre P. Chimeric extracellular domain type II transforming growth factor (TGF)-beta receptor fused to the Fc region of human immunoglobulin as a TGF-beta antagonist. Eur J Biochem. 1998 Jun 15;254(3):505–513. doi: 10.1046/j.1432-1327.1998.2540505.x. [DOI] [PubMed] [Google Scholar]
  27. Kopp J. B., Factor V. M., Mozes M., Nagy P., Sanderson N., Böttinger E. P., Klotman P. E., Thorgeirsson S. S. Transgenic mice with increased plasma levels of TGF-beta 1 develop progressive renal disease. Lab Invest. 1996 Jun;74(6):991–1003. [PubMed] [Google Scholar]
  28. Kulkarni A. B., Huh C. G., Becker D., Geiser A., Lyght M., Flanders K. C., Roberts A. B., Sporn M. B., Ward J. M., Karlsson S. Transforming growth factor beta 1 null mutation in mice causes excessive inflammatory response and early death. Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):770–774. doi: 10.1073/pnas.90.2.770. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Laiho M., Saksela O., Keski-Oja J. Transforming growth factor-beta induction of type-1 plasminogen activator inhibitor. Pericellular deposition and sensitivity to exogenous urokinase. J Biol Chem. 1987 Dec 25;262(36):17467–17474. [PubMed] [Google Scholar]
  30. Lin H. Y., Moustakas A., Knaus P., Wells R. G., Henis Y. I., Lodish H. F. The soluble exoplasmic domain of the type II transforming growth factor (TGF)-beta receptor. A heterogeneously glycosylated protein with high affinity and selectivity for TGF-beta ligands. J Biol Chem. 1995 Feb 10;270(6):2747–2754. doi: 10.1074/jbc.270.6.2747. [DOI] [PubMed] [Google Scholar]
  31. Massagué J. Receptors for the TGF-beta family. Cell. 1992 Jun 26;69(7):1067–1070. doi: 10.1016/0092-8674(92)90627-o. [DOI] [PubMed] [Google Scholar]
  32. McAnulty R. J., Campa J. S., Cambrey A. D., Laurent G. J. The effect of transforming growth factor beta on rates of procollagen synthesis and degradation in vitro. Biochim Biophys Acta. 1991 Jan 31;1091(2):231–235. doi: 10.1016/0167-4889(91)90066-7. [DOI] [PubMed] [Google Scholar]
  33. Miller G. T., Hochman P. S., Meier W., Tizard R., Bixler S. A., Rosa M. D., Wallner B. P. Specific interaction of lymphocyte function-associated antigen 3 with CD2 can inhibit T cell responses. J Exp Med. 1993 Jul 1;178(1):211–222. doi: 10.1084/jem.178.1.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Miller R. L., Udenfriend S. Hydroxylation of proline residues in collagen nascent chains. Arch Biochem Biophys. 1970 Jul;139(1):104–113. doi: 10.1016/0003-9861(70)90051-2. [DOI] [PubMed] [Google Scholar]
  35. Penttinen R. P., Kobayashi S., Bornstein P. Transforming growth factor beta increases mRNA for matrix proteins both in the presence and in the absence of changes in mRNA stability. Proc Natl Acad Sci U S A. 1988 Feb;85(4):1105–1108. doi: 10.1073/pnas.85.4.1105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Qi Z., Atsuchi N., Ooshima A., Takeshita A., Ueno H. Blockade of type beta transforming growth factor signaling prevents liver fibrosis and dysfunction in the rat. Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2345–2349. doi: 10.1073/pnas.96.5.2345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Raghow R., Lurie S., Seyer J. M., Kang A. H. Profiles of steady state levels of messenger RNAs coding for type I procollagen, elastin, and fibronectin in hamster lungs undergoing bleomycin-induced interstitial pulmonary fibrosis. J Clin Invest. 1985 Nov;76(5):1733–1739. doi: 10.1172/JCI112163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Raghow R., Postlethwaite A. E., Keski-Oja J., Moses H. L., Kang A. H. Transforming growth factor-beta increases steady state levels of type I procollagen and fibronectin messenger RNAs posttranscriptionally in cultured human dermal fibroblasts. J Clin Invest. 1987 Apr;79(4):1285–1288. doi: 10.1172/JCI112950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Raghow R. The role of extracellular matrix in postinflammatory wound healing and fibrosis. FASEB J. 1994 Aug;8(11):823–831. doi: 10.1096/fasebj.8.11.8070631. [DOI] [PubMed] [Google Scholar]
  40. Raghu G., Masta S., Meyers D., Narayanan A. S. Collagen synthesis by normal and fibrotic human lung fibroblasts and the effect of transforming growth factor-beta. Am Rev Respir Dis. 1989 Jul;140(1):95–100. doi: 10.1164/ajrccm/140.1.95. [DOI] [PubMed] [Google Scholar]
  41. Roberts A. B., Sporn M. B., Assoian R. K., Smith J. M., Roche N. S., Wakefield L. M., Heine U. I., Liotta L. A., Falanga V., Kehrl J. H. Transforming growth factor type beta: rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen formation in vitro. Proc Natl Acad Sci U S A. 1986 Jun;83(12):4167–4171. doi: 10.1073/pnas.83.12.4167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Roberts A. B., Sporn M. B. Physiological actions and clinical applications of transforming growth factor-beta (TGF-beta). Growth Factors. 1993;8(1):1–9. doi: 10.3109/08977199309029129. [DOI] [PubMed] [Google Scholar]
  43. Santana A., Saxena B., Noble N. A., Gold L. I., Marshall B. C. Increased expression of transforming growth factor beta isoforms (beta 1, beta 2, beta 3) in bleomycin-induced pulmonary fibrosis. Am J Respir Cell Mol Biol. 1995 Jul;13(1):34–44. doi: 10.1165/ajrcmb.13.1.7541221. [DOI] [PubMed] [Google Scholar]
  44. Sanvito F., Nichols A., Herrera P. L., Huarte J., Wohlwend A., Vassalli J. D., Orci L. TGF-beta 1 overexpression in murine pancreas induces chronic pancreatitis and, together with TNF-alpha, triggers insulin-dependent diabetes. Biochem Biophys Res Commun. 1995 Dec 26;217(3):1279–1286. doi: 10.1006/bbrc.1995.2906. [DOI] [PubMed] [Google Scholar]
  45. Sime P. J., Xing Z., Graham F. L., Csaky K. G., Gauldie J. Adenovector-mediated gene transfer of active transforming growth factor-beta1 induces prolonged severe fibrosis in rat lung. J Clin Invest. 1997 Aug 15;100(4):768–776. doi: 10.1172/JCI119590. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Snider G. L., Celli B. R., Goldstein R. H., O'Brien J. J., Lucey E. C. Chronic interstitial pulmonary fibrosis produced in hamsters by endotracheal bleomycin. Lung volumes, volume-pressure relations, carbon monoxide uptake, and arterial blood gas studied. Am Rev Respir Dis. 1978 Feb;117(2):289–297. doi: 10.1164/arrd.1978.117.2.289. [DOI] [PubMed] [Google Scholar]
  47. Tsang M. L., Zhou L., Zheng B. L., Wenker J., Fransen G., Humphrey J., Smith J. M., O'Connor-McCourt M., Lucas R., Weatherbee J. A. Characterization of recombinant soluble human transforming growth factor-beta receptor type II (rhTGF-beta sRII). Cytokine. 1995 Jul;7(5):389–397. doi: 10.1006/cyto.1995.0054. [DOI] [PubMed] [Google Scholar]
  48. Vivien D., Attisano L., Wrana J. L., Massagué J. Signaling activity of homologous and heterologous transforming growth factor-beta receptor kinase complexes. J Biol Chem. 1995 Mar 31;270(13):7134–7141. doi: 10.1074/jbc.270.13.7134. [DOI] [PubMed] [Google Scholar]
  49. WOESSNER J. F., Jr The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch Biochem Biophys. 1961 May;93:440–447. doi: 10.1016/0003-9861(61)90291-0. [DOI] [PubMed] [Google Scholar]
  50. Wang Q. J., Giri S. N., Hyde D. M., Li C. Amelioration of bleomycin-induced pulmonary fibrosis in hamsters by combined treatment with taurine and niacin. Biochem Pharmacol. 1991 Aug 8;42(5):1115–1122. [PubMed] [Google Scholar]
  51. Wang Q., Hyde D. M., Giri S. N. Abatement of bleomycin-induced increases in vascular permeability, inflammatory cell infiltration, and fibrotic lesions in hamster lungs by combined treatment with taurine and niacin. Lab Invest. 1992 Aug;67(2):234–242. [PubMed] [Google Scholar]
  52. Weissler J. C. Idiopathic pulmonary fibrosis: cellular and molecular pathogenesis. Am J Med Sci. 1989 Feb;297(2):91–104. doi: 10.1097/00000441-198902000-00005. [DOI] [PubMed] [Google Scholar]
  53. Wrana J. L., Attisano L., Wieser R., Ventura F., Massagué J. Mechanism of activation of the TGF-beta receptor. Nature. 1994 Aug 4;370(6488):341–347. doi: 10.1038/370341a0. [DOI] [PubMed] [Google Scholar]
  54. Yamamoto T., Noble N. A., Cohen A. H., Nast C. C., Hishida A., Gold L. I., Border W. A. Expression of transforming growth factor-beta isoforms in human glomerular diseases. Kidney Int. 1996 Feb;49(2):461–469. doi: 10.1038/ki.1996.65. [DOI] [PubMed] [Google Scholar]
  55. di Clemente N., Wilson C., Faure E., Boussin L., Carmillo P., Tizard R., Picard J. Y., Vigier B., Josso N., Cate R. Cloning, expression, and alternative splicing of the receptor for anti-Müllerian hormone. Mol Endocrinol. 1994 Aug;8(8):1006–1020. doi: 10.1210/mend.8.8.7997230. [DOI] [PubMed] [Google Scholar]

Articles from Thorax are provided here courtesy of BMJ Publishing Group

RESOURCES