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. 2001 Jan;56(1):25–29. doi: 10.1136/thorax.56.1.25

Ultrastructural examination of bronchial biopsy specimens from children with moderate asthma

H Cokugras 1, N Akcakaya 1, I Seckin 1, Y Camcioglu 1, N Sarimurat 1, F Aksoy 1
PMCID: PMC1745903  PMID: 11120900

Abstract

BACKGROUND—Few studies have evaluated the asthmatic airway in childhood. The aim of this study was to assess the histopathological changes occurring in the bronchi of children with moderate asthma using light and electron microscopy.
METHODS—Bronchial biopsy specimens from 10 children with moderate asthma (seven boys) of mean (SD) age 9.3 (3.8) years (range 5-14) were examined by light and electron microscopy. Patients had not had a respiratory infection for at least one month and they had not been treated with steroids or sodium cromoglycate for four weeks before the study. Bronchoscopy was performed under general anaesthesia using a Karl Storz rigid paediatric bronchoscope. Biopsy materials were stained with uracyl acetate and lead citrate and evaluated under a Zeiss-10 electron microscope and light microscope.
RESULTS—The most important finding was thickening and hyalinisation of the basement membrane in nine patients. The ciliated epithelial cells showed loss of cilia in some cases. Overactive fibroblasts were consistently found. Six patients had degranulating mast cells and lymphocyte infiltration in the submucosa. Eosinophils were seen in only one biopsy sample.
CONCLUSION—Children with moderate asthma develop bronchial inflammation similar to the reaction observed in adults. However, in our study the inflammation was rich in lymphocytes rather than eosinophils.



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Selected References

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  1. Beasley R., Burgess C., Crane J., Pearce N., Roche W. Pathology of asthma and its clinical implications. J Allergy Clin Immunol. 1993 Jul;92(1 Pt 2):148–154. doi: 10.1016/0091-6749(93)90097-y. [DOI] [PubMed] [Google Scholar]
  2. Chu H. W., Halliday J. L., Martin R. J., Leung D. Y., Szefler S. J., Wenzel S. E. Collagen deposition in large airways may not differentiate severe asthma from milder forms of the disease. Am J Respir Crit Care Med. 1998 Dec;158(6):1936–1944. doi: 10.1164/ajrccm.158.6.9712073. [DOI] [PubMed] [Google Scholar]
  3. Dukes M. N., Holgate S. T., Pauwels R. A. Report of an international workshop on risk and safety of asthma therapy. Clin Exp Allergy. 1994 Feb;24(2):160–165. doi: 10.1111/j.1365-2222.1994.tb00214.x. [DOI] [PubMed] [Google Scholar]
  4. Elias J. A., Zhu Z., Chupp G., Homer R. J. Airway remodeling in asthma. J Clin Invest. 1999 Oct;104(8):1001–1006. doi: 10.1172/JCI8124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GLYNN A. A., MICHAELS L. Bronchial biopsy in chronic bronchitis and asthma. Tip Fak Mecm. 1960 Jun;23:142–153. doi: 10.1136/thx.15.2.142. [DOI] [PubMed] [Google Scholar]
  6. Gizycki M. J., Adelroth E., Rogers A. V., O'Byrne P. M., Jeffery P. K. Myofibroblast involvement in the allergen-induced late response in mild atopic asthma. Am J Respir Cell Mol Biol. 1997 Jun;16(6):664–673. doi: 10.1165/ajrcmb.16.6.9191468. [DOI] [PubMed] [Google Scholar]
  7. Haczku A., Moqbel R., Elwood W., Sun J., Kay A. B., Barnes P. J., Chung K. F. Effects of prolonged repeated exposure to ovalbumin in sensitized brown Norway rats. Am J Respir Crit Care Med. 1994 Jul;150(1):23–27. doi: 10.1164/ajrccm.150.1.8025754. [DOI] [PubMed] [Google Scholar]
  8. Holgate S. T. The 1992 Cournand Lecture. Asthma: past, present and future. Eur Respir J. 1993 Nov;6(10):1507–1520. [PubMed] [Google Scholar]
  9. Humbert M., Robinson D. S., Assoufi B., Kay A. B., Durham S. R. Safety of fibreoptic bronchoscopy in asthmatic and control subjects and effect on asthma control over two weeks. Thorax. 1996 Jul;51(7):664–669. doi: 10.1136/thx.51.7.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jeffery P. K., Wardlaw A. J., Nelson F. C., Collins J. V., Kay A. B. Bronchial biopsies in asthma. An ultrastructural, quantitative study and correlation with hyperreactivity. Am Rev Respir Dis. 1989 Dec;140(6):1745–1753. doi: 10.1164/ajrccm/140.6.1745. [DOI] [PubMed] [Google Scholar]
  11. Lacoste J. Y., Bousquet J., Chanez P., Van Vyve T., Simony-Lafontaine J., Lequeu N., Vic P., Enander I., Godard P., Michel F. B. Eosinophilic and neutrophilic inflammation in asthma, chronic bronchitis, and chronic obstructive pulmonary disease. J Allergy Clin Immunol. 1993 Oct;92(4):537–548. doi: 10.1016/0091-6749(93)90078-t. [DOI] [PubMed] [Google Scholar]
  12. Laitinen A., Laitinen L. A. Pathology of asthma. Allergy Proc. 1994 Nov-Dec;15(6):323–328. doi: 10.2500/108854194778816436. [DOI] [PubMed] [Google Scholar]
  13. Laitinen L. A., Heino M., Laitinen A., Kava T., Haahtela T. Damage of the airway epithelium and bronchial reactivity in patients with asthma. Am Rev Respir Dis. 1985 Apr;131(4):599–606. doi: 10.1164/arrd.1985.131.4.599. [DOI] [PubMed] [Google Scholar]
  14. Laitinen L. A., Heino M., Laitinen A., Kava T., Haahtela T. Damage of the airway epithelium and bronchial reactivity in patients with asthma. Am Rev Respir Dis. 1985 Apr;131(4):599–606. doi: 10.1164/arrd.1985.131.4.599. [DOI] [PubMed] [Google Scholar]
  15. Laitinen L. A., Laitinen A., Haahtela T. Airway mucosal inflammation even in patients with newly diagnosed asthma. Am Rev Respir Dis. 1993 Mar;147(3):697–704. doi: 10.1164/ajrccm/147.3.697. [DOI] [PubMed] [Google Scholar]
  16. Perez C. R., Wood R. E. Update on pediatric flexible bronchoscopy. Pediatr Clin North Am. 1994 Apr;41(2):385–400. doi: 10.1016/s0031-3955(16)38727-2. [DOI] [PubMed] [Google Scholar]
  17. Raine J., Warner J. O. Fibreoptic bronchoscopy without general anaesthetic. Arch Dis Child. 1991 Apr;66(4):481–484. doi: 10.1136/adc.66.4.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ray A., Cohn L. Th2 cells and GATA-3 in asthma: new insights into the regulation of airway inflammation. J Clin Invest. 1999 Oct;104(8):985–993. doi: 10.1172/JCI8204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Remes S. T., Korppi M., Remes K., Pekkanen J. Prevalence of asthma at school age: a clinical population-based study in eastern Finland. Acta Paediatr. 1996 Jan;85(1):59–63. doi: 10.1111/j.1651-2227.1996.tb13891.x. [DOI] [PubMed] [Google Scholar]
  20. Roche W. R., Beasley R., Williams J. H., Holgate S. T. Subepithelial fibrosis in the bronchi of asthmatics. Lancet. 1989 Mar 11;1(8637):520–524. doi: 10.1016/s0140-6736(89)90067-6. [DOI] [PubMed] [Google Scholar]
  21. Schneider R. C., Zapol W. M., Carvalho A. C. Platelet consumption and sequestration in severe acute respiratory failure. Am Rev Respir Dis. 1980 Sep;122(3):445–451. doi: 10.1164/arrd.1980.122.3.445. [DOI] [PubMed] [Google Scholar]
  22. Söderberg M., Hellström S., Sandström T., Lundgren R., Bergh A. Structural characterization of bronchial mucosal biopsies from healthy volunteers: a light and electron microscopical study. Eur Respir J. 1990 Mar;3(3):261–266. [PubMed] [Google Scholar]
  23. Vignola A. M., Chanez P., Campbell A. M., Souques F., Lebel B., Enander I., Bousquet J. Airway inflammation in mild intermittent and in persistent asthma. Am J Respir Crit Care Med. 1998 Feb;157(2):403–409. doi: 10.1164/ajrccm.157.2.96-08040. [DOI] [PubMed] [Google Scholar]
  24. Warner J. O., Naspitz C. K. Third International Pediatric Consensus statement on the management of childhood asthma. International Pediatric Asthma Consensus Group. Pediatr Pulmonol. 1998 Jan;25(1):1–17. doi: 10.1002/(sici)1099-0496(199801)25:1<1::aid-ppul1>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]

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