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. 2002 Apr;57(4):357–360. doi: 10.1136/thorax.57.4.357

γδ T lymphocytes in the peripheral blood of patients with tuberculosis with and without HIV co-infection

A Carvalho, A Matteelli, P Airo, S Tedoldi, C Casalini, L Imberti, G Cadeo, A Beltrame, G Carosi
PMCID: PMC1746296  PMID: 11923557

Abstract

Background: Several recent studies suggest that γδ T lymphocytes play an important role in immunity against Mycobacterium tuberculosis. However, the dynamics of these cells in the peripheral blood of patients with tuberculosis (TB) with and without HIV infection is not fully understood. A study was undertaken to evaluate the profile of the γδ T cell population in patients at the time the diagnosis of TB was established.

Methods: A cross sectional study was performed in consecutive TB patients from the Department of Infectious Diseases, Spedali Civili, Brescia. CD4+, CD8+ and Vδ1 and Vδ2 T cell counts were analysed. Lymphocyte surface membrane expression was evaluated with the FITC-TCRγδ, -Vδ1, -Vδ2 and PE-Vδ1 monoclonal antibodies. Blood donors and HIV seropositive asymptomatic individuals acted as controls.

Results: Seventy four TB patients were evaluated, 20 of whom (27%) were co-infected with HIV. HIV seronegative TB patients (n=54) had total γδ T cells and Vδ1 subsets comparable to those in blood donors (n=39). However, the percentage with the Vδ2 subset was significantly lower in patients with TB than in controls (median 1.5 v 2.1; p=0.05). Responsiveness to PPD was not associated with predominance of a specific γδ T cell subset. HIV seropositive individuals had a decreased percentage of circulating Vδ2 cells at a level similar to that in HIV seronegative TB patients, regardless of the presence of active TB.

Conclusions: HIV seronegative TB patients and HIV infected individuals (with or without active TB) have a reduced number of circulating Vδ2 T cells compared with healthy individuals. Whether TB and HIV infection share a common mechanism causing Vδ2 T cell depletion still needs to be established.

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Selected References

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  1. Autran B., Triebel F., Katlama C., Rozenbaum W., Hercend T., Debre P. T cell receptor gamma/delta+ lymphocyte subsets during HIV infection. Clin Exp Immunol. 1989 Feb;75(2):206–210. [PMC free article] [PubMed] [Google Scholar]
  2. Balbi B., Valle M. T., Oddera S., Giunti D., Manca F., Rossi G. A., Allegra L. T-lymphocytes with gamma delta+ V delta 2+ antigen receptors are present in increased proportions in a fraction of patients with tuberculosis or with sarcoidosis. Am Rev Respir Dis. 1993 Dec;148(6 Pt 1):1685–1690. doi: 10.1164/ajrccm/148.6_Pt_1.1685. [DOI] [PubMed] [Google Scholar]
  3. Barnes P. F., Grisso C. L., Abrams J. S., Band H., Rea T. H., Modlin R. L. Gamma delta T lymphocytes in human tuberculosis. J Infect Dis. 1992 Mar;165(3):506–512. doi: 10.1093/infdis/165.3.506. [DOI] [PubMed] [Google Scholar]
  4. Boullier S., Cochet M., Poccia F., Gougeon M. L. CDR3-independent gamma delta V delta 1+ T cell expansion in the peripheral blood of HIV-infected persons. J Immunol. 1995 Feb 1;154(3):1418–1431. [PubMed] [Google Scholar]
  5. De Maria A., Ferrazin A., Ferrini S., Ciccone E., Terragna A., Moretta L. Selective increase of a subset of T cell receptor gamma delta T lymphocytes in the peripheral blood of patients with human immunodeficiency virus type 1 infection. J Infect Dis. 1992 May;165(5):917–919. doi: 10.1093/infdis/165.5.917. [DOI] [PubMed] [Google Scholar]
  6. Dieli F., Sireci G., Di Sano C., Romano A., Titone L., Di Carlo P., Ivanyi J., Fourniè J. J., Salerno A. Ligand-specific alphabeta and gammadelta T cell responses in childhood tuberculosis. J Infect Dis. 2000 Jan;181(1):294–301. doi: 10.1086/315180. [DOI] [PubMed] [Google Scholar]
  7. Ellner J. J. Suppressor adherent cells in human tuberculosis. J Immunol. 1978 Dec;121(6):2573–2579. [PubMed] [Google Scholar]
  8. Falini B., Flenghi L., Pileri S., Pelicci P., Fagioli M., Martelli M. F., Moretta L., Ciccone E. Distribution of T cells bearing different forms of the T cell receptor gamma/delta in normal and pathological human tissues. J Immunol. 1989 Oct 15;143(8):2480–2488. [PubMed] [Google Scholar]
  9. Ito M., Kojiro N., Ikeda T., Ito T., Funada J., Kokubu T. Increased proportions of peripheral blood gamma delta T cells in patients with pulmonary tuberculosis. Chest. 1992 Jul;102(1):195–197. doi: 10.1378/chest.102.1.195. [DOI] [PubMed] [Google Scholar]
  10. Kabelitz D., Bender A., Prospero T., Wesselborg S., Janssen O., Pechhold K. The primary response of human gamma/delta + T cells to Mycobacterium tuberculosis is restricted to V gamma 9-bearing cells. J Exp Med. 1991 Jun 1;173(6):1331–1338. doi: 10.1084/jem.173.6.1331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Li B., Bassiri H., Rossman M. D., Kramer P., Eyuboglu A. F., Torres M., Sada E., Imir T., Carding S. R. Involvement of the Fas/Fas ligand pathway in activation-induced cell death of mycobacteria-reactive human gamma delta T cells: a mechanism for the loss of gamma delta T cells in patients with pulmonary tuberculosis. J Immunol. 1998 Aug 1;161(3):1558–1567. [PubMed] [Google Scholar]
  12. Li B., Rossman M. D., Imir T., Oner-Eyuboglu A. F., Lee C. W., Biancaniello R., Carding S. R. Disease-specific changes in gammadelta T cell repertoire and function in patients with pulmonary tuberculosis. J Immunol. 1996 Nov 1;157(9):4222–4229. [PubMed] [Google Scholar]
  13. Poccia F., Boullier S., Lecoeur H., Cochet M., Poquet Y., Colizzi V., Fournie J. J., Gougeon M. L. Peripheral V gamma 9/V delta 2 T cell deletion and anergy to nonpeptidic mycobacterial antigens in asymptomatic HIV-1-infected persons. J Immunol. 1996 Jul 1;157(1):449–461. [PubMed] [Google Scholar]
  14. Poquet Y., Halary F., Champagne E., Davodeau F., Gougeon M. L., Bonneville M., Fournié J. J. Human gamma delta T cells in tuberculosis. Res Immunol. 1996 Oct-Dec;147(8-9):542–549. doi: 10.1016/s0923-2494(97)85220-0. [DOI] [PubMed] [Google Scholar]
  15. Tazi A., Bouchonnet F., Valeyre D., Cadranel J., Battesti J. P., Hance A. J. Characterization of gamma/delta T-lymphocytes in the peripheral blood of patients with active tuberculosis. A comparison with normal subjects and patients with sarcoidosis. Am Rev Respir Dis. 1992 Nov;146(5 Pt 1):1216–1221. doi: 10.1164/ajrccm/146.5_Pt_1.1216. [DOI] [PubMed] [Google Scholar]
  16. Ueta C., Tsuyuguchi I., Kawasumi H., Takashima T., Toba H., Kishimoto S. Increase of gamma/delta T cells in hospital workers who are in close contact with tuberculosis patients. Infect Immun. 1994 Dec;62(12):5434–5441. doi: 10.1128/iai.62.12.5434-5441.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wesch D., Kabelitz D., Friese K., Pechhold K. Mycobacteria-reactive gamma delta T cells in HIV-infected individuals: lack of V gamma 9 cell responsiveness is due to deficiency of antigen-specific CD4 T helper type 1 cells. Eur J Immunol. 1996 Mar;26(3):557–562. doi: 10.1002/eji.1830260309. [DOI] [PubMed] [Google Scholar]

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