Abstract
Methods: Three 60 ml samples of bronchoalveolar lavage (BAL) fluid were taken from nine stable lung transplant recipients 3–12 months after transplantation. Detection of AHLs was carried out on dichloromethane extracted supernatants using the bioluminescence based AHL reporter plasmid pSB1075. This responds to the presence of AHLs with long acyl chains (C10–C14), generating light. Synthetic AHLs were included as positive controls.
Results: Five of the nine BAL fluid supernatants exhibited AHL activity, suggesting the presence of AHLs with long N-acyl chains. There was no correlation between the levels of AHLs detected or their absence and BAL fluid microbiology or diagnosis before transplantation.
Conclusions: This is the first evidence for the presence of AHL quorum sensing signals in human lung allograft recipients, even in subjects with no rejection or apparent infection. Further longitudinal follow up of these preliminary findings is required to elucidate potential links with infection, rejection, and allograft deterioration.
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Selected References
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- Favre-Bonté Sabine, Pache Jean-Claude, Robert John, Blanc Dominique, Pechère Jean-Claude, van Delden Christian. Detection of Pseudomonas aeruginosa cell-to-cell signals in lung tissue of cystic fibrosis patients. Microb Pathog. 2002 Mar;32(3):143–147. doi: 10.1006/mpat.2001.0487. [DOI] [PubMed] [Google Scholar]
- Hasan A., Corris P. A., Healy M., Wrightson N., Gascoigne A. D., Waller D. A., Wilson I., Hilton C. J., Gould F. K., Forty J. Bilateral sequential lung transplantation for end stage septic lung disease. Thorax. 1995 May;50(5):565–566. doi: 10.1136/thx.50.5.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Middleton Barry, Rodgers Helen C., Cámara Miguel, Knox Alan J., Williams Paul, Hardman Andrea. Direct detection of N-acylhomoserine lactones in cystic fibrosis sputum. FEMS Microbiol Lett. 2002 Jan 22;207(1):1–7. doi: 10.1111/j.1574-6968.2002.tb11019.x. [DOI] [PubMed] [Google Scholar]
- Sharples Linda D., McNeil Keith, Stewart Susan, Wallwork John. Risk factors for bronchiolitis obliterans: a systematic review of recent publications. J Heart Lung Transplant. 2002 Feb;21(2):271–281. doi: 10.1016/s1053-2498(01)00360-6. [DOI] [PubMed] [Google Scholar]
- Singh P. K., Schaefer A. L., Parsek M. R., Moninger T. O., Welsh M. J., Greenberg E. P. Quorum-sensing signals indicate that cystic fibrosis lungs are infected with bacterial biofilms. Nature. 2000 Oct 12;407(6805):762–764. doi: 10.1038/35037627. [DOI] [PubMed] [Google Scholar]
- Steinman Michael A., Landefeld C. Seth, Gonzales Ralph. Predictors of broad-spectrum antibiotic prescribing for acute respiratory tract infections in adult primary care. JAMA. 2003 Feb 12;289(6):719–725. doi: 10.1001/jama.289.6.719. [DOI] [PubMed] [Google Scholar]
- Telford G., Wheeler D., Williams P., Tomkins P. T., Appleby P., Sewell H., Stewart G. S., Bycroft B. W., Pritchard D. I. The Pseudomonas aeruginosa quorum-sensing signal molecule N-(3-oxododecanoyl)-L-homoserine lactone has immunomodulatory activity. Infect Immun. 1998 Jan;66(1):36–42. doi: 10.1128/iai.66.1.36-42.1998. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ward C., Snell G. I., Orsida B., Zheng L., Williams T. J., Walters E. H. Airway versus transbronchial biopsy and BAL in lung transplant recipients: different but complementary. Eur Respir J. 1997 Dec;10(12):2876–2880. doi: 10.1183/09031936.97.10122876. [DOI] [PubMed] [Google Scholar]
- Ward C., Snell G. I., Zheng L., Orsida B., Whitford H., Williams T. J., Walters E. H. Endobronchial biopsy and bronchoalveolar lavage in stable lung transplant recipients and chronic rejection. Am J Respir Crit Care Med. 1998 Jul;158(1):84–91. doi: 10.1164/ajrccm.158.1.9707117. [DOI] [PubMed] [Google Scholar]
- Ward C., Walters E. H., Zheng L., Whitford H., Williams T. J., Snell G. I. Increased soluble CD14 in bronchoalveolar lavage fluid of stable lung transplant recipients. Eur Respir J. 2002 Mar;19(3):472–478. doi: 10.1183/09031936.02.00225502. [DOI] [PubMed] [Google Scholar]
- Williams P., Camara M., Hardman A., Swift S., Milton D., Hope V. J., Winzer K., Middleton B., Pritchard D. I., Bycroft B. W. Quorum sensing and the population-dependent control of virulence. Philos Trans R Soc Lond B Biol Sci. 2000 May 29;355(1397):667–680. doi: 10.1098/rstb.2000.0607. [DOI] [PMC free article] [PubMed] [Google Scholar]