Skip to main content
PMC home page
Thorax logoLink to Thorax
. 2004 Jun;59(6):526–528. doi: 10.1136/thx.2003.010801

Prevalence and clonality of Burkholderia cepacia complex genomovars in UK patients with cystic fibrosis referred for lung transplantation

A De Soyza 1, K Morris 1, A McDowell 1, C Doherty 1, L Archer 1, J Perry 1, J Govan 1, P Corris 1, K Gould 1
PMCID: PMC1747049  PMID: 15170040

Abstract

Background: It has previously been reported that patients infected with Burkholderia cenocepacia (genomovar III) before lung transplantation have a poorer outcome than those with other B cepacia complex infections.

Methods: An extensive study was conducted to determine the prevalence and clonality of B cepacia complex genomovars isolated from patients referred for transplant assessment between 1989 to the present and, where appropriate, whether strain type was related to transplant outcome.

Results: Isolates from 29 patients were identified as B cepacia complex organisms by molecular analysis. Thirteen patients (45%) were infected with the highly transmissible ET-12 strain of B cenocepacia recA lineage III-A, while all remaining patients were infected with genetically unique B cenocepacia, B multivorans, and B vietnamiensis strains. All previously reported deaths following transplantation were associated with ET-12 infection.

Conclusions: The ET-12 strain is the predominant cause of B cenocepacia infections in patients with cystic fibrosis referred to our pulmonary transplant centre and is associated with poor transplant outcomes using standard treatment regimens.

Full Text

The Full Text of this article is available as a PDF (113.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agodi A., Barchitta M., Gianninò V., Collura A., Pensabene T., Garlaschi M. L., Pasquarella C., Luzzaro F., Sinatra F., Mahenthiralingam E. Burkholderia cepacia complex in cystic fibrosis and non-cystic fibrosis patients: identification of a cluster of epidemic lineages. J Hosp Infect. 2002 Mar;50(3):188–195. doi: 10.1053/jhin.2001.1160. [DOI] [PubMed] [Google Scholar]
  2. Aris R. M., Routh J. C., LiPuma J. J., Heath D. G., Gilligan P. H. Lung transplantation for cystic fibrosis patients with Burkholderia cepacia complex. Survival linked to genomovar type. Am J Respir Crit Care Med. 2001 Dec 1;164(11):2102–2106. doi: 10.1164/ajrccm.164.11.2107022. [DOI] [PubMed] [Google Scholar]
  3. Chaparro C., Maurer J., Gutierrez C., Krajden M., Chan C., Winton T., Keshavjee S., Scavuzzo M., Tullis E., Hutcheon M. Infection with Burkholderia cepacia in cystic fibrosis: outcome following lung transplantation. Am J Respir Crit Care Med. 2001 Jan;163(1):43–48. doi: 10.1164/ajrccm.163.1.9811076. [DOI] [PubMed] [Google Scholar]
  4. De Soyza A., McDowell A., Archer L., Dark J. H., Elborn S. J., Mahenthiralingam E., Gould K., Corris P. A. Burkholderia cepacia complex genomovars and pulmonary transplantation outcomes in patients with cystic fibrosis. Lancet. 2001 Nov 24;358(9295):1780–1781. doi: 10.1016/S0140-6736(01)06808-8. [DOI] [PubMed] [Google Scholar]
  5. Hasan A., Corris P. A., Healy M., Wrightson N., Gascoigne A. D., Waller D. A., Wilson I., Hilton C. J., Gould F. K., Forty J. Bilateral sequential lung transplantation for end stage septic lung disease. Thorax. 1995 May;50(5):565–566. doi: 10.1136/thx.50.5.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heath David G., Hohneker Kathy, Carriker Charlene, Smith Kelly, Routh Jonathan, LiPuma John J., Aris Robert M., Weber David, Gilligan Peter H. Six-year molecular analysis of Burkholderia cepacia complex isolates among cystic fibrosis patients at a referral center for lung transplantation. J Clin Microbiol. 2002 Apr;40(4):1188–1193. doi: 10.1128/JCM.40.4.1188-1193.2002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Isles A., Maclusky I., Corey M., Gold R., Prober C., Fleming P., Levison H. Pseudomonas cepacia infection in cystic fibrosis: an emerging problem. J Pediatr. 1984 Feb;104(2):206–210. doi: 10.1016/s0022-3476(84)80993-2. [DOI] [PubMed] [Google Scholar]
  8. Mahenthiralingam E., Bischof J., Byrne S. K., Radomski C., Davies J. E., Av-Gay Y., Vandamme P. DNA-Based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. J Clin Microbiol. 2000 Sep;38(9):3165–3173. doi: 10.1128/jcm.38.9.3165-3173.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mahenthiralingam E., Simpson D. A., Speert D. P. Identification and characterization of a novel DNA marker associated with epidemic Burkholderia cepacia strains recovered from patients with cystic fibrosis. J Clin Microbiol. 1997 Apr;35(4):808–816. doi: 10.1128/jcm.35.4.808-816.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mahenthiralingam Eshwar, Baldwin Adam, Vandamme Peter. Burkholderia cepacia complex infection in patients with cystic fibrosis. J Med Microbiol. 2002 Jul;51(7):533–538. doi: 10.1099/0022-1317-51-7-533. [DOI] [PubMed] [Google Scholar]
  11. Perry John D., Riley Gavin, Johnston Susan, Dark John H., Gould F. Kate. Activity of disinfectants against Gram-negative bacilli isolated from patients undergoing lung transplantation for cystic fibrosis. J Heart Lung Transplant. 2002 Nov;21(11):1230–1231. doi: 10.1016/s1053-2498(02)00434-5. [DOI] [PubMed] [Google Scholar]
  12. Sajjan U. S., Sun L., Goldstein R., Forstner J. F. Cable (cbl) type II pili of cystic fibrosis-associated Burkholderia (Pseudomonas) cepacia: nucleotide sequence of the cblA major subunit pilin gene and novel morphology of the assembled appendage fibers. J Bacteriol. 1995 Feb;177(4):1030–1038. doi: 10.1128/jb.177.4.1030-1038.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sajjan U., Wu Y., Kent G., Forstner J. Preferential adherence of cable-piliated burkholderia cepacia to respiratory epithelia of CF knockout mice and human cystic fibrosis lung explants. J Med Microbiol. 2000 Oct;49(10):875–885. doi: 10.1099/0022-1317-49-10-875. [DOI] [PubMed] [Google Scholar]
  14. Steinbach S., Sun L., Jiang R. Z., Flume P., Gilligan P., Egan T. M., Goldstein R. Transmissibility of Pseudomonas cepacia infection in clinic patients and lung-transplant recipients with cystic fibrosis. N Engl J Med. 1994 Oct 13;331(15):981–987. doi: 10.1056/NEJM199410133311504. [DOI] [PubMed] [Google Scholar]
  15. Vandamme Peter, Holmes Barry, Coenye Tom, Goris Johan, Mahenthiralingam Eshwar, LiPuma John J., Govan John R. W. Burkholderia cenocepacia sp. nov.--a new twist to an old story. Res Microbiol. 2003 Mar;154(2):91–96. doi: 10.1016/S0923-2508(03)00026-3. [DOI] [PubMed] [Google Scholar]

Articles from Thorax are provided here courtesy of BMJ Publishing Group

RESOURCES