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. 2004 Sep;59(9):769–772. doi: 10.1136/thx.2003.012930

Antitussive activity of iodo-resiniferatoxin in guinea pigs

M Trevisani 1, A Milan 1, R Gatti 1, A Zanasi 1, S Harrison 1, G Fontana 1, A Morice 1, P Geppetti 1
PMCID: PMC1747129  PMID: 15333853

Abstract

Background: Iodo-resiniferatoxin (I-RTX) has recently been described as an ultra potent antagonist of the transient receptor potential vanilloid-1 (TRPV1).

Methods: The ability of I-RTX to inhibit cough induced by inhalation of two putative TRPV1 stimulants (capsaicin and citric acid) was tested in non-anaesthetised guinea pigs.

Results: Pretreatment with I-RTX either intraperitoneally (0.03–0.3 µmol/kg) or by aerosol (0.1–3 µM) reduced the number of coughs produced by inhalation of citric acid (0.25 M) and capsaicin (30 µM) in a dose dependent manner. Capsazepine (CPZ) also reduced citric acid and capsaicin induced cough, but the activity of I-RTX was 10–100 times more potent than CPZ in all the experimental conditions tested.

Conclusions: I-RTX is a novel and potent antitussive drug which inhibits cough mediated by agents possibly acting via TRPV1 activation.

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