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. 2005 Jan;60(1):22–26. doi: 10.1136/thx.2003.017327

Exhaled breath condensate pH and exhaled nitric oxide in allergic asthma and in cystic fibrosis

J Ojoo 1, S Mulrennan 1, J Kastelik 1, A Morice 1, A Redington 1
PMCID: PMC1747154  PMID: 15618578

Abstract

Background: It has been proposed that the pH of airway lining fluid may regulate the fractional exhaled concentration of nitric oxide (FENO) in respiratory disease.

Methods: FENO, exhaled breath condensate (EBC) pH, and EBC concentrations of nitrite plus nitrate (NO2/NO3) were compared in 12 subjects with stable asthma, 18 with stable cystic fibrosis (CF), and 15 healthy control subjects. Eight of the CF patients were studied on a separate occasion at the start of a pulmonary exacerbation.

Results: FENO was significantly greater in asthmatic subjects than in control subjects (mean 35 v 9 ppb, p<0.001). EBC pH, however, was similar in the asthmatic and control groups (median 5.82 v 6.08, p = 0.23). Levels of NO2/NO3 were on average higher in EBC samples from asthmatic subjects, but the difference was not significant. In patients with stable CF both the FENO (mean 4 ppb, p<0.001) and EBC pH (median 5.77, p = 0.003) were lower than in the control group. Levels of EBC NO2/NO3 (median 29.9 µM; p = 0.002) in patients with stable CF, in contrast, were significantly higher than in control subjects. During CF exacerbations, EBC pH was further reduced (median 5.30, p = 0.017) but FENO and NO2/NO3 were unchanged.

Conclusions: These findings demonstrate a dissociation between EBC pH and FENO in inflammatory airways disease.

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Selected References

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